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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/872652.rdf" xlink:actuate="onRequest">Initial Methane Concentration Alteration Experiment Data of the Deep Santa Barbara Basin Water Column from October 2019 (BASIN project)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Qin, Q., Valentine, D. L., Treude, T., Kinnaman, F. S., Gosselin, K. M., Liu, N. (2022) Initial Methane Concentration Alteration Experiment Data of the Deep Santa Barbara Basin Water Column from October 2019 (BASIN project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-04-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.872652.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Methodology:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sampling and analytical procedures:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Methane concentrations were measured by headspace equilibration method, modified from Kinnaman et al., 2007.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Fractional methane turnover rates were analyzed based on the 3H-labeled methane incubation protocol by Bussmann et al., 2015.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Methane oxidation rates were calculated assuming adherence to the first-order rate law, by multiplying fractional methane turnover rate and ambient methane concentration (Valentine et al., 2001). &amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples were collected from R/V Connell.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/764255.rdf" xlink:title="OCE-1756947" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756947 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756947</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/764261.rdf" xlink:title="OCE-1756667" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756667 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756667</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/764265.rdf" xlink:title="OCE-1756242" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756242 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756242</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/810830.rdf" xlink:title="OCE-1829981" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829981 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829981</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/810835.rdf" xlink:title="OCE-1830033" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1830033 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1830033</gmx:Anchor>
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&lt;p&gt;The dynamics of oxygen minimum zones along continental margins, and their potential for future expansion, are important because of their intersection with global biogeochemical cycles and because of their far-reaching impacts on ocean ecosystems. However, the impacts of transient deoxygenation on biogeochemical cycles of carbon, nitrogen and sulfur at the sea floor are not well established and are the focus of this study. This study will test the overarching hypothesis that deoxygenation triggers a positive feedback loop between bacterial mats at the sea floor that consume hydrogen sulfide, a sulfur species that can be toxic to higher organisms, and an underlying community of bacteria that produce hydrogen sulfide. By this hypothesis, the establishment of sea floor mats, which depend on inorganic nitrogen sources to run their sulfur metabolism, accelerates nitrogen cycling in the uppermost sediment horizon following deoxygenation. The accelerated nitrogen cycling allows for upward expansion of the sulfide-producing bacteria, which in-turn provide a shallow source of sulfide as substrate to further support nitrogen cycling in the sea floor mat. The results of this study will enable understanding of the relationship between oxygen dynamics in the water column and the biogeochemical processes at the sea floor that link the transformations of carbon, nitrogen and sulfur. The results of this study promise to define the environmental conditions under which the sulfur and nitrogen cycles are coupled and subject to strong positive feedbacks at the seafloor, as well as the conditions under which they are decoupled. This study provides training in research and innovative analytical and experimental techniques to four graduate students and several undergraduates. Undergraduates will be engaged in research at two institutions, one of which has recently been designated as a Hispanic serving institution. Approximately 10 undergraduate students (20 in total) will participate in each of the two proposed oceanographic expeditions, through an established course entitled: Field Studies in Marine Biogeochemistry. This course provides an opportunity for students to develop an independent research project in advance of the expedition, to participate on the expedition, and to conduct research projects while at sea.&lt;/p&gt;
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&amp;lt;p&amp;gt;Methane oxidation rates were calculated assuming adherence to the first-order rate law, by multiplying fractional methane turnover rate and ambient methane concentration (Valentine et al., 2001). &amp;lt;/p&amp;gt;

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