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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/873459.rdf" xlink:actuate="onRequest">Aquaria water quality total alkalinity, DIC, and CO2 measurements from full factorial study of Acropora cervicornis at Mote Marine Laboratory in Nov-Dec 2019</gmx:Anchor>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Water quality was monitored as part of a full&amp;amp;nbsp;factorial experiment to determine the survival probability and photochemical efficiency of 25 unique genotypes of&amp;amp;nbsp;&amp;lt;em&amp;gt;Acropora&amp;amp;nbsp;cervicornis&amp;amp;nbsp;&amp;lt;/em&amp;gt;in November and December 2019.&amp;amp;nbsp; Coral fragments of&amp;amp;nbsp;&amp;lt;em&amp;gt;A.&amp;amp;nbsp;cervicornis&amp;amp;nbsp;&amp;lt;/em&amp;gt;coral fragments were exposed to&amp;amp;nbsp;high temperatures and/or high&amp;amp;nbsp;&amp;lt;em&amp;gt;p&amp;lt;/em&amp;gt;CO2&amp;amp;nbsp;treatments&amp;amp;nbsp;in flow-through tanks at&amp;amp;nbsp;Mote Marine Laboratory.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;During the exposure period, water quality for each tank was measured daily and color index of the coral fragments was assessed.&amp;amp;nbsp; Water samples were collected in acid-washed amber bottles (125 mL) for carbonate chemistry analysis bi-weekly and fixed with mercuric chloride (60 µL). Water samples were filtered (0.2 microns) prior to analysis on the dissolved inorganic carbon (DIC) machine (Apollo SciTech Analyzer).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Treatment tank water quality was monitored using a YSI Professional Plus (Pro Plus) Multi-parameter handheld with a quarto containing a Pro Series Galvanic Dissolved Oxygen Sensor, a Pro Series pH Sensor (calibrated using 4, 7, and 10 buffers), and a Pro Series temperature and conductivity sensor.&amp;amp;nbsp;&amp;amp;nbsp;Dissolved inorganic carbon (DIC) was analyzed using the Apollo SciTech DIC analyzer model AS-C151. Total Alkalinity (TA) was analyzed using the Metrohm 905 Titrando analyzer. Both DIC and TA were standardized each day with certified reference material (CRM) provided by A.G. Dickson. The coral health chart/ color index card was provided by CoralWatch.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Details on additional&amp;amp;nbsp;water quality measurements can be found in these datasets:&amp;lt;/p&amp;gt;

&amp;lt;ul&amp;gt;
&amp;lt;li&amp;gt;Acer Aquaria water quality pH and DO,&amp;amp;nbsp;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/873433&amp;quot; style=&amp;quot;margin: 0px; padding: 0px; border: 0px; font: inherit; vertical-align: baseline; text-decoration-line: none; color: rgb(0, 102, 153);&amp;quot;&amp;gt;https://www.bco-dmo.org/dataset/873433&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;Acer Aquaria water quality PAR,&amp;amp;nbsp;https://www.bco-dmo.org/dataset/873446&amp;lt;/li&amp;gt;
&amp;lt;/ul&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/642851.rdf" xlink:title="OCE-1452538" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1452538</gmx:Anchor>
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Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.&lt;/p&gt;
&lt;p&gt;&lt;em&gt;Acropora cervicornis&lt;/em&gt; dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify &lt;em&gt;A. cervicornis&lt;/em&gt; genotypes resistant to disease, 2) identify &lt;em&gt;A. cervicornis&lt;/em&gt; genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on &lt;em&gt;A. cervicornis&lt;/em&gt;; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.&lt;/p&gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;During the exposure period, water quality for each tank was measured daily and color index of the coral fragments was assessed.&amp;amp;nbsp; Water samples were collected in acid-washed amber bottles (125 mL) for carbonate chemistry analysis bi-weekly and fixed with mercuric chloride (60 µL). Water samples were filtered (0.2 microns) prior to analysis on the dissolved inorganic carbon (DIC) machine (Apollo SciTech Analyzer).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Treatment tank water quality was monitored using a YSI Professional Plus (Pro Plus) Multi-parameter handheld with a quarto containing a Pro Series Galvanic Dissolved Oxygen Sensor, a Pro Series pH Sensor (calibrated using 4, 7, and 10 buffers), and a Pro Series temperature and conductivity sensor.&amp;amp;nbsp;&amp;amp;nbsp;Dissolved inorganic carbon (DIC) was analyzed using the Apollo SciTech DIC analyzer model AS-C151. Total Alkalinity (TA) was analyzed using the Metrohm 905 Titrando analyzer. Both DIC and TA were standardized each day with certified reference material (CRM) provided by A.G. Dickson. The coral health chart/ color index card was provided by CoralWatch.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Details on additional&amp;amp;nbsp;water quality measurements can be found in these datasets:&amp;lt;/p&amp;gt;

&amp;lt;ul&amp;gt;
&amp;lt;li&amp;gt;Acer Aquaria water quality pH and DO,&amp;amp;nbsp;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/873433&amp;quot; style=&amp;quot;margin: 0px; padding: 0px; border: 0px; font: inherit; vertical-align: baseline; text-decoration-line: none; color: rgb(0, 102, 153);&amp;quot;&amp;gt;https://www.bco-dmo.org/dataset/873433&amp;lt;/a&amp;gt;&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;Acer Aquaria water quality PAR,&amp;amp;nbsp;https://www.bco-dmo.org/dataset/873446&amp;lt;/li&amp;gt;
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              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>treatment tank</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: treatment tank PI Supplied Instrument Description:Aquaria water quality was measured in tanks used for coral treatment experiments Instrument Name: Aquarium Instrument Short Name:Aquarium   Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/743387.rdf" xlink:title="Inorganic Carbon Analyzer" xlink:actuate="onRequest">Apollo SciTech DIC analyzer model AS-C151</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Apollo SciTech DIC analyzer model AS-C151</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Apollo SciTech DIC analyzer model AS-C151 PI Supplied Instrument Description:Dissolved inorganic carbon (DIC) was analyzed using the Apollo SciTech DIC analyzer model AS-C151.  Instrument Name: Inorganic Carbon Analyzer Instrument Short Name:   Instrument Description: Instruments measuring carbonate in sediments and inorganic carbon (including DIC) in the water column.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/682.rdf" xlink:title="Titrator" xlink:actuate="onRequest">Metrohm 905 Titrando analyzer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Metrohm 905 Titrando analyzer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Metrohm 905 Titrando analyzer PI Supplied Instrument Description:Total Alkalinity (TA) was analyzed using the Metrohm 905 Titrando analyzer.  Instrument Name: Titrator Instrument Short Name:Titrator   Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/666.rdf" xlink:title="YSI Professional Plus Multi-Parameter Probe" xlink:actuate="onRequest">YSI Professional Plus (Pro Plus) Multi-parameter handheld</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>YSI Professional Plus (Pro Plus) Multi-parameter handheld</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: YSI Professional Plus (Pro Plus) Multi-parameter handheld PI Supplied Instrument Description:Treatment tank water quality was monitored using a YSI Professional Plus (Pro Plus) Multi-parameter handheld with a quarto containing a Pro Series Galvanic Dissolved Oxygen Sensor, a Pro Series pH Sensor, and a Pro Series temperature and conductivity sensor Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus   Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
