http://lod.bco-dmo.org/id/dataset/874597
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-05-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Measurements of respiration, net photosynthesis, and gross photosynthesis of five different genotypes of Symbiodinium microadriaticum in culture at three different temperatures
2022-05-24
publication
2022-05-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-05-31
publication
https://doi.org/10.26008/1912/bco-dmo.874597.1
Casey terHorst
California State University Northridge
principalInvestigator
Jennica Moffat
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: terHorst, C., Moffat, J. (2022) Measurements of respiration, net photosynthesis, and gross photosynthesis of five different genotypes of Symbiodinium microadriaticum in culture at three different temperatures. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-05-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.874597.1 [access date]
Symbiodinium microadriaticum cultures Dataset Description: Methods and Sampling: <p>To investigate the physiological responses of isolated symbiont genotypes to temperature, we grew replicate cultures of each of the five genotypes in growth chambers set to 26°C, 30°C, and 32°C. The mean temperatures (+/- 1 s.d.) in the three chambers were 25.5°C (+/- 0.5), 30.1°C (+/- 0.3), and 31.6°C (+/- 0.2). We initiated 12 replicate cultures of each genotype in sterile flasks with 75 milliliters (mL) of sterile f/2 media with 750,000 cells (10,000 cells/mL) from the appropriate stock culture. Replicate cultures of each genotype were randomly distributed among three identical growth chambers (Percival I-36LLVL) set to each of the three temperatures (n=4 replicate cultures of each genotype at each temperature). We systematically rotated the position of cultures in the growth chamber daily to minimize the effect of any small differences in light and temperature within the chamber. Lights were set on a 12:12 day:night cycle, with an average illumination during the day of 4533 (+/- 456) Lux. We performed this experiment in October 2019.</p>
<p>We measured respiration and photosynthetic rate using a SDR SensorDish Reader (Loligo Systems, Viborg, Denmark). We filled two wells with 2 mL sampled from each culture and filled two wells with DI water as controls. We placed plates in each of the three growth chambers set to 26°, 30°, and 32° C and dark-acclimated plates for five minutes before measuring oxygen concentration every 15 seconds for 15 minutes in the dark. We then turned on the lights in each growth chamber and measured oxygen concentration again in the same way. We also quantified algal density using the average of four replicate hemocytometer counts.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559105 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559105
completed
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
Jennica Moffat
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Temperature
Genotype
Flask
CellsPermL
Respiration
NetPhoto
GrossPhoto
RespPer1000
NetPhotoPer1000
GrossPhotoPer1000
SDR SensorDish Reader (Loligo Systems, Viborg, Denmark)
theme
None, User defined
temperature of sample or a generic measurement (not in-situ water temp)
sample identification
replicate
cell_concentration
respiration rate
photosynthetic rate
featureType
BCO-DMO Standard Parameters
Respirometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://osprey.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
oceans
2019-10-01
2019-10-31
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Measurements of respiration, net photosynthesis, and gross photosynthesis of five different genotypes of Symbiodinium microadriaticum in culture at three different temperatures
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/875114.rdf
Name: Temperature
Units: degrees celsius
Description: <p>Growth temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/875115.rdf
Name: Genotype
Units: unitless
Description: <p>One of five algal genotypes</p>
http://lod.bco-dmo.org/id/dataset-parameter/875116.rdf
Name: Flask
Units: unitless
Description: <p>Flask number</p>
http://lod.bco-dmo.org/id/dataset-parameter/875117.rdf
Name: CellsPermL
Units: cells per mL
Description: <p>Number of cells per milliliter</p>
http://lod.bco-dmo.org/id/dataset-parameter/875118.rdf
Name: Respiration
Units: micromoles of oxygen per minute (umolO2/min)
Description: <p>Total respiration</p>
http://lod.bco-dmo.org/id/dataset-parameter/875119.rdf
Name: NetPhoto
Units: micromoles of oxygen per minute (umolO2/min)
Description: <p>Net photosynthesis</p>
http://lod.bco-dmo.org/id/dataset-parameter/875120.rdf
Name: GrossPhoto
Units: micromoles of oxygen per minute (umolO2/min)
Description: <p>Gross photosynthesis</p>
http://lod.bco-dmo.org/id/dataset-parameter/875121.rdf
Name: RespPer1000
Units: micromoles of oxygen per minute per 1000 cells (umolO2/min/1000cells)
Description: <p>Respiration per 1000 cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/875122.rdf
Name: NetPhotoPer1000
Units: micromoles of oxygen per minute per 1000 cells (umolO2/min/1000cells)
Description: <p>Net photosynthesis per 1000 cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/875123.rdf
Name: GrossPhotoPer1000
Units: micromoles of oxygen per minute per 1000 cells (umolO2/min/1000cells)
Description: <p>Gross photosynthesis per 1000 cells</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4304
https://darchive.mblwhoilibrary.org/bitstream/1912/28944/1/dataset-874597_culture-data__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.874597.1
download
onLine
dataset
<p>To investigate the physiological responses of isolated symbiont genotypes to temperature, we grew replicate cultures of each of the five genotypes in growth chambers set to 26°C, 30°C, and 32°C. The mean temperatures (+/- 1 s.d.) in the three chambers were 25.5°C (+/- 0.5), 30.1°C (+/- 0.3), and 31.6°C (+/- 0.2). We initiated 12 replicate cultures of each genotype in sterile flasks with 75 milliliters (mL) of sterile f/2 media with 750,000 cells (10,000 cells/mL) from the appropriate stock culture. Replicate cultures of each genotype were randomly distributed among three identical growth chambers (Percival I-36LLVL) set to each of the three temperatures (n=4 replicate cultures of each genotype at each temperature). We systematically rotated the position of cultures in the growth chamber daily to minimize the effect of any small differences in light and temperature within the chamber. Lights were set on a 12:12 day:night cycle, with an average illumination during the day of 4533 (+/- 456) Lux. We performed this experiment in October 2019.</p>
<p>We measured respiration and photosynthetic rate using a SDR SensorDish Reader (Loligo Systems, Viborg, Denmark). We filled two wells with 2 mL sampled from each culture and filled two wells with DI water as controls. We placed plates in each of the three growth chambers set to 26°, 30°, and 32° C and dark-acclimated plates for five minutes before measuring oxygen concentration every 15 seconds for 15 minutes in the dark. We then turned on the lights in each growth chamber and measured oxygen concentration again in the same way. We also quantified algal density using the average of four replicate hemocytometer counts.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
We calculated respiration rates for each well as the slope of the best-fit line to the decline in oxygen concentration over time in the dark. We subtracted the slope of the same fit in the control wells to account for any background noise. We averaged the two replicate wells for each culture and standardized respiration by cell number. We determined net photosynthesis in the same manner, using the slope of the best-fit line to the increase in oxygen concentration over time. Finally, we calculated gross photosynthesis by adding the absolute value of respiration to net photosynthesis for each culture. We used multiple general linear models with type III SS to test for the effects of algal genotype, temperature, and their interaction on respiration, gross photosynthesis, and net photosynthesis. All variables were transformed to meet assumptions of normality and homoscedasticity (respiration: cube root; gross and net photosynthesis: fourth root).</p>
<p><strong>BCO-DMO Processing:</strong><br />
- Added a conventional header with dataset name, PI names, version date</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SDR SensorDish Reader (Loligo Systems, Viborg, Denmark)
SDR SensorDish Reader (Loligo Systems, Viborg, Denmark)
PI Supplied Instrument Name: SDR SensorDish Reader (Loligo Systems, Viborg, Denmark) Instrument Name: Respirometer Instrument Short Name: Instrument Description: A device that measures the rate of respiration by a living organism or organic system by measuring its rate of exchange of oxygen and/or carbon dioxide.