http://lod.bco-dmo.org/id/dataset/874609
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-05-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Responses of polyps (survival, inoculation time, time to strobilation, time to ephyra production, bud production, ephyra production) to exposure to five different algal symbiont genotypes
2022-05-31
publication
2022-05-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-05-31
publication
https://doi.org/10.26008/1912/bco-dmo.874609.1
Casey terHorst
California State University Northridge
principalInvestigator
Jennica Moffat
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: terHorst, C., Moffat, J. (2022) Responses of polyps (survival, inoculation time, time to strobilation, time to ephyra production, bud production, ephyra production) to exposure to five different algal symbiont genotypes. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-05-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.874609.1 [access date]
Polyp Fitness Dataset Description: Methods and Sampling: <p>We inoculated aposymbiotic Cassiopea xamachana clones with one of the five genotypes of <em>S. microadriaticum</em> and maintained them at 26°, 30°, and 32°C for 28 days. We also maintained replicates of aposymbiotic polyps at each temperature as a control. For 26 days, we measured the survival of each polyp, as well as asexual reproduction and developmental timing.</p>
<p>After four days of acclimation to temperature (i.e., Day 1), we supplied polyps with access to one of the five genotypes of <em>S. microadriaticum</em>, while also maintaining control polyps with no symbionts. Each well was inoculated with 24,000 cells (2,000 cells/mL) from the appropriate stock algal culture. We fed polyps with Artemia nauplii immediately prior to symbiont inoculation because symbiont uptake occurs more readily when polyps are feeding. We inoculated four six-well plates with each of the five algal genotypes, plus a no algae control, at each temperature, resulting in 24 replicate wells for each genotype by temperature combination (N=432 polyps). We inoculated wells on days 1, 4, 11, 17, 20, and 23 using the same density of cells from the same stock cultures each time. We visually inspected polyps daily under a dissecting microscope to determine survival. When a polyp died, the polyp was removed and the well was emptied. We quantified two strategies of asexual reproduction as the total number of buds and total number of ephyra produced. Buds that were produced during the experiment remained in the well; most buds settled and metamorphosed into polyps, but the experiment was not long enough to allow any newly produced buds to become inoculated and strobilate. We removed all ephyrae that were produced during the experiment the day they detached from the parent polyp. We also measured three developmental timing events: time to inoculation, time to strobilation, and time to ephyra release. Aposymbiotic polyps are white and appear brown once inoculated with algae, so polyps were considered inoculated when a brown tint was observable under the dissecting microscope. Polyps were considered to have begun strobilating when they became disc-shaped rather than cone-shaped. The time to ephyra release was marked as the day that the ephyra detached from the parent bud.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559105 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559105
completed
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
Jennica Moffat
pointOfContact
asNeeded
Dataset Version: 1
Unknown
WellNum
Genotype
Temp
Plate
DayDead
Survive_to_End
Days_to_Inoculation
Days_to_Strobilation
Days_to_Ephyra
TotalBuds
TotalEphyra
Leica compound microscope
theme
None, User defined
sample identification
temperature of sample or a generic measurement (not in-situ water temp)
day of month
comments
days
number
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://osprey.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
oceans
2019-12-01
2019-12-31
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Responses of polyps (survival, inoculation time, time to strobilation, time to ephyra production, bud production, ephyra production) to exposure to five different algal symbiont genotypes
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/875097.rdf
Name: WellNum
Units: unitless
Description: <p>Plate well number</p>
http://lod.bco-dmo.org/id/dataset-parameter/875098.rdf
Name: Genotype
Units: unitless
Description: <p>One of five algal genotypes</p>
http://lod.bco-dmo.org/id/dataset-parameter/875099.rdf
Name: Temp
Units: degrees celisius
Description: <p>Growth temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/875100.rdf
Name: Plate
Units: unitless
Description: <p>Plate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/875101.rdf
Name: DayDead
Units: unitless
Description: <p>Day on which a polyp died</p>
http://lod.bco-dmo.org/id/dataset-parameter/875102.rdf
Name: Survive_to_End
Units: unitless
Description: <p>Binary survival (yes or no)</p>
http://lod.bco-dmo.org/id/dataset-parameter/875103.rdf
Name: Days_to_Inoculation
Units: unitless
Description: <p>Number of days until inoculation</p>
http://lod.bco-dmo.org/id/dataset-parameter/875104.rdf
Name: Days_to_Strobilation
Units: unitless
Description: <p>Number of days until strobilation</p>
http://lod.bco-dmo.org/id/dataset-parameter/875105.rdf
Name: Days_to_Ephyra
Units: unitless
Description: <p>Number of days until first ephyra</p>
http://lod.bco-dmo.org/id/dataset-parameter/875106.rdf
Name: TotalBuds
Units: unitless
Description: <p>Number of buds produced</p>
http://lod.bco-dmo.org/id/dataset-parameter/875107.rdf
Name: TotalEphyra
Units: unitless
Description: <p>Number of ephyrase produced</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15736
https://darchive.mblwhoilibrary.org/bitstream/1912/28945/1/dataset-874609_polyp-fitness__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.874609.1
download
onLine
dataset
<p>We inoculated aposymbiotic Cassiopea xamachana clones with one of the five genotypes of <em>S. microadriaticum</em> and maintained them at 26°, 30°, and 32°C for 28 days. We also maintained replicates of aposymbiotic polyps at each temperature as a control. For 26 days, we measured the survival of each polyp, as well as asexual reproduction and developmental timing.</p>
<p>After four days of acclimation to temperature (i.e., Day 1), we supplied polyps with access to one of the five genotypes of <em>S. microadriaticum</em>, while also maintaining control polyps with no symbionts. Each well was inoculated with 24,000 cells (2,000 cells/mL) from the appropriate stock algal culture. We fed polyps with Artemia nauplii immediately prior to symbiont inoculation because symbiont uptake occurs more readily when polyps are feeding. We inoculated four six-well plates with each of the five algal genotypes, plus a no algae control, at each temperature, resulting in 24 replicate wells for each genotype by temperature combination (N=432 polyps). We inoculated wells on days 1, 4, 11, 17, 20, and 23 using the same density of cells from the same stock cultures each time. We visually inspected polyps daily under a dissecting microscope to determine survival. When a polyp died, the polyp was removed and the well was emptied. We quantified two strategies of asexual reproduction as the total number of buds and total number of ephyra produced. Buds that were produced during the experiment remained in the well; most buds settled and metamorphosed into polyps, but the experiment was not long enough to allow any newly produced buds to become inoculated and strobilate. We removed all ephyrae that were produced during the experiment the day they detached from the parent polyp. We also measured three developmental timing events: time to inoculation, time to strobilation, and time to ephyra release. Aposymbiotic polyps are white and appear brown once inoculated with algae, so polyps were considered inoculated when a brown tint was observable under the dissecting microscope. Polyps were considered to have begun strobilating when they became disc-shaped rather than cone-shaped. The time to ephyra release was marked as the day that the ephyra detached from the parent bud.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
We used multiple general linear mixed effects models to test for the effects of algal genotype, temperature, and their interaction on total bud production and total ephyra production. Bud production data met assumptions of normality and homoscedasticity and ephyra production was square-root transformed to meet assumptions. Because Cassiopea will not produce ephyra without symbionts, we removed the aposymbiotic group for analyses of ephyra production and the developmental timing events below. Because not all polyps reached each developmental stage, we used a hurdle model approach to examine whether algal genotypes, temperature, and their interaction affected (a) development to each development stage (survival, successful inoculation, strobilation, and ephyra production) and (b) the time for successful individuals to reach that stage. To determine the effects of each factor on reaching each developmental stage, we used generalized linear models with a binomial error distribution, removing individuals who did not reach a previous stage when analyzing progress to the next stage. Then we used additional general linear mixed effects models to test the effects of the same factors on the time to reach each stage, again removing individuals that did not reach a particular stage. Data were transformed to meet model assumptions (inoculation and strobilation: log; ephyra: Box-Cox transformation). To account for any variation among replicate plates, in all models we included plate as a random effect, but removed the random effect when it did not increase model fit (determined by AIC).&nbsp; All models were fit using lm or lmer (or glm and glmer for binomial error distributions) in the ‘lme4’ package in R (v. 4.0.3). For mixed models, we tested the significance of fixed effects with Likelihood Ratio Tests; in the absence of random effects, we tested fixed effects using Anova in the ‘car’ package.</p>
<p><strong>BCO-DMO Processing: </strong><br />
- Missing data identifier ‘NA’ replaced with 'nd' (BCO-DMO's default missing data identifier);<br />
- Added a conventional header with dataset name, PI names, version date<br />
- Rounded column "r" to three decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Leica compound microscope
Leica compound microscope
PI Supplied Instrument Name: Leica compound microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/