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            <gco:CharacterString>Cite this dataset as: Dorgan, K., Clemo, W. C. (2025) Sediment surface elevation, erodibility, and acoustic properties of sediments collected from the Northern Gulf of Mexico following laboratory resuspension at the Dauphin Island Sea Lab in 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-08-25 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.875373.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Sediment resuspension summary Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Sediment cores were collected from 10 meters (m) depth in the northern Gulf of Mexico offshore of Dauphin Island, Alabama, in cohesive muddy sediment on January 26, 2020 (30° 13.333' N, 88° 8.348' W). Cores were collected from a Dauphin Island Sea Lab vessel, the R/V Alabama Discovery. Polycarbonate sediment cores (9.6 centimeter (cm) inner diameter x 60 cm height) were collected using an MC-400 multicorer (Ocean Instruments, Fall City, WA). We resuspended the surface 5 cm of the cores in the lab using a modified baster with holes that directed water jets laterally below the sediment surface. We compared temporal changes in sediment compaction to changes in surface and subsurface cohesion over 30 days post-resuspension.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sediment-water interface (SWI) height and acoustic sound speed through sediment, which depends on bulk density, provided continuous and nondestructive metrics of compaction, and sediment porosity and grain size were measured destructively to characterize sediment physical structure. We determined surface cohesion by measuring both eroded mass and turbidity resulting from increasing shear stress. Subsurface cohesion was determined from the force required for sediments to fail in tension. We compared surface and subsurface exopolymeric substance (EPS) concentrations to surface and subsurface cohesion measurements. We differentiated between water-soluble (colloidal) and sediment-bound EPS as we expected bound EPS to contribute more to sediment-organic matrix development and thus cohesion because they are directly bound to sediment grains rather than dissolved in porewater.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;These data include the summary of data collected on cores processed over time points 0 days (no resuspension), then 1, 2, 3, 7, 14, and 30 days post resuspension. There were 5 replicate cores per time point, but not all data were collected on each replicate core. Detailed data on erosion measurements, as well as repeated non-destructive measurements of sediment-water interface height and sound speed on cores processed on day 30 are provided in separate datasets.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Cores were extruded and sliced at 1 cm depth intervals. We calculated water content from sediment mass differences before and after drying at 65 degrees Celsius (°C) for 24 hours as mass of water divided by mass of dry sediment (Eq. 4.7 from Jackson and Richardson 2007). We measured grain size every cm in the top 5 cm and at 8 and 10 cm for undisturbed cores and cores 3 and 30 days after resuspension. We determined grain size distribution with a Malvern Mastersizer 3000 particle analyzer (Malvern Panalytical, Malvern, UK) and data were analyzed with Gradistat (Kenneth Pye Associates, Ltd., Berkshire, UK) and classified according to Folk and Ward (1957).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We performed acoustic measurements following methods from Dorgan et al. (2020). Within a seawater tank, a 400 kHz three-cycle sinusoidal tone burst was transmitted horizontally through sediment cores to a receiver at 3 depths below the sediment surface (2.5, 5, 10 cm) (see Fig. 1 in Dorgan et al., 2020). To account for sound speed differences due to temporal variability in temperature and salinity, sound speed through sediment was normalized by the sound speed in seawater to obtain sound speed ratio (SSR). Each day, we also performed acoustic measurements on cores filled with seawater and with no core present. Sound speed in seawater and the lag time between the transmitted and received signals (time of flight) through sediment and seawater cores were used to calculate sound speed in sediment (νp):&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;ν_p=c_w/(1-(c_w * ∆t/d_s )&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;where cw is sound speed in water, Δt is the difference in time of flight between seawater core (tw) and sediment core (ts), and ds is the inner diameter of the core (Jackson and Richardson, 2007; Dorgan et al., 2020). SSR was then calculated by dividing νp by cw, where a higher SSR indicates more compact sediment.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To determine if differences in erodibility and tensile strength were driven by variability in surface and subsurface EPS, we analyzed the subcore used for water content and grain size measurements for EPS carbohydrate concentrations. Following methods of de Brouwer and Stal (2001), we lyophilized frozen sediment and extracted colloidal carbohydrates with purified water (E-Pure) for 1 hour at 30 °C. We then extracted bound carbohydrates with 0.1 M Na2EDTA for 16 hours at room temperature. We measured both carbohydrate fractions with the sulfuric acid-UV assay (Albalasmeh et al., 2013), which is based on the phenol-sulfuric acid assay (DuBois et al., 1956). 900 µL 96 % sulfuric acid was added to 300 microliters (µL) carbohydrate solution to dehydrate dissolved carbohydrates into furfural derivatives, which absorb UV light. This solution was vortexed for 30 seconds, allowed to return to room temperature for approximately 5 minutes, then UV absorbance at 315 nanometers (nm) was measured using a SpectraMax M5 microplate reader (Molecular Devices). We determined carbohydrate concentration from UV absorbance of a glucose reference.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To determine subsurface cohesion changes over time, we measured tensile force (N) using a custom probe modified from a fracture toughness probe developed by Johnson et al. (2012). A helical probe is rotated and translated into the sediment like a corkscrew, then pulled upward, breaking off a plug of sediment. Force, measured with an in-line force sensor (Futek LS-200 2-lb), increases to a peak force, then drops when the plug breaks free of the sediment below. Forces from friction with the surrounding sediment and the weight of the sediment plug are removed by repeating the corkscrew motion and subtracting the force profile from the second upward pull. The peak force in the plot of net force as a function of upward distance corresponds to the tensile strength of the sediment, a metric of cohesion. Fracture toughness can be calculated from this peak force (Johnson et al. 2012), but due to some concerns about the effect of sediment depth on these calculations (Dorgan, unpublished data), only force is presented here. These force measurements are comparable across the same depth in different cores, with higher force indicating greater cohesion.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Instruments:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Grain size analysis was done on a Malvern Mastersizer 3000 particle analyzer. Most instruments are custom built. Acoustics measurements were done following Dorgan et al. 2020, JASA. Other measurements are described in Clemo et al., 2022.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/811715.rdf" xlink:title="OCE-1844910" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1844910 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1844910</gmx:Anchor>
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Marine sediments are important habitats for abundant and diverse communities of organisms that are important as food sources for higher trophic levels, including commercially important species. Through burrowing, constructing tubes, and feeding on sediments, these animals modify their physical and chemical environments to such an extent that they are considered ecosystem engineers. Bioturbation, the mixing of sediments by animals, is important in regenerating nutrients and transporting pollutants and carbon bound to mineral grains. Despite its importance, our ability to predict bioturbation rates and patterns from the community structure is poor, largely due to a lack of understanding of the mechanisms by which animals mix sediments. This project builds on earlier work showing that animals extend burrows through muddy sediments by fracture to test the hypothesis that the mechanical properties of sediments that affect burrowing mechanics also affect sediment mixing. More broadly, this project examines the relative contributions of (i) the functional roles of the organisms in the community, (ii) the mechanical properties of sediments, and (iii) factors that might increase or decrease animal activity such as temperature and food availability to bioturbation rates. Burrowing animals modify the physical properties of sediments, and this project quantifies these changes and tests the hypothesis that these changes are ecologically important and affect community succession following a disturbance. In addition to this scientific broader impact, this project involves development of instrumentation to measure sediment properties and includes a substantial education plan to introduce graduate, undergraduate, and middle school students to the important role that technology plays in marine science.&lt;/p&gt;
&lt;p&gt;Through burrowing and feeding activities, benthic infauna mix sediments and modify their physical environments. Bioturbation gates the burial of organic matter, enhances nutrient regeneration, and smears the paleontological and stratigraphic record. However, current understanding of the mechanisms by which infaunal activities mix sediments is insufficient to predict the impacts of changes in infaunal community structure on important sediment ecosystem functions driven by bioturbation. This project tests specific hypotheses relating infaunal communities, bioturbation, and geotechnical properties with the ultimate goal of understanding the dynamic changes and potential feedbacks between infauna and their physical environments. This project integrates field and lab experiments to assess the relative importance of infaunal community structure and activities to bioturbation rates. Additionally, this project builds on recent work showing that muddy sediments are elastic gels through which worms extend burrows by fracture to propose that geotechnical properties of sediments mediate bioturbation by governing the release of particles from the sediment matrix during burrow extension. Finite element modeling determines how the release of particles by fracture during burrowing depends on the fracture toughness (cohesion) and stiffness (compaction) of sediments and complements laboratory experiments characterizing the impact of geotechnical properties on burrowing behaviors. The proposed research also aims to determine whether impacts of infauna on geotechnical properties are ecologically important. Changes in infaunal communities and geotechnical properties following an experimental physical disturbance address the hypothesis that ecosystem engineering of bulk sediment properties facilitates succession.&lt;/p&gt;
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            http://lod.bco-dmo.org/id/dataset-parameter/983316.rdf
	Name: coreID
	Units: unitless
	Description: &lt;p&gt;core ID named as &amp;quot;D_samplingday(max30)_replicate(A-E)&amp;quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983317.rdf
	Name: latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of sample collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983318.rdf
	Name: longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude of sample collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983319.rdf
	Name: water_depth_m
	Units: meters (m)
	Description: &lt;p&gt;Water depth in meters&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983320.rdf
	Name: date
	Units: unitless
	Description: &lt;p&gt;Date of sample collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983321.rdf
	Name: time_hour
	Units: hours
	Description: &lt;p&gt;time in hours since sediment disturbance was performed&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983322.rdf
	Name: time_day
	Units: days
	Description: &lt;p&gt;time in days since sediment disturbance was performed&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983323.rdf
	Name: timepoint
	Units: unitless
	Description: &lt;p&gt;time point of experiment (max 30)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983324.rdf
	Name: replicate
	Units: unitless
	Description: &lt;p&gt;replicate identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983325.rdf
	Name: depth_cm
	Units: centimeters (cm)
	Description: &lt;p&gt;depth within core&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983326.rdf
	Name: water_content_fraction
	Units: unitless
	Description: &lt;p&gt;(wet mass - dry mass)/dry mass of sediment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983327.rdf
	Name: density_seawater
	Units: kilograms per cubic meter (kg m-3)
	Description: &lt;p&gt;density of seawater&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983328.rdf
	Name: density_sediment
	Units: kilograms per cubic meter (kg m-3)
	Description: &lt;p&gt;density of sediment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983329.rdf
	Name: soundspeed_ratio_mean
	Units: unitless
	Description: &lt;p&gt;ratio of sound speed in mud to sound speed in water&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983330.rdf
	Name: CumErodedMass
	Units: kilograms per square meter (kg m-2)
	Description: &lt;p&gt;mass of eroded material from gust chamber&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983331.rdf
	Name: time_turbiditysensor_maxedout
	Units: minutes
	Description: &lt;p&gt;amount of time the reading from the turbidity sensor was maxed out&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983332.rdf
	Name: timefraction_turbiditysensor_maxedout
	Units: unitless
	Description: &lt;p&gt;fraction of time the turbidity sensor was maxed out&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983333.rdf
	Name: EPScolloidal
	Units: micrograms per gram (ug g-1)
	Description: &lt;p&gt;amount of colloidal exopolymeric substances in sediment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983334.rdf
	Name: EPSsedimentbound
	Units: micrograms per gram (ug g-1)
	Description: &lt;p&gt;amount of sediment bound exopolymeric substances in sediment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983335.rdf
	Name: MaxTensileF_N
	Units: newtons (N)
	Description: &lt;p&gt;max tensile force measured with custom probe (2 lb force sensor)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983336.rdf
	Name: GrainSizeAvgd10
	Units: micrometers (um)
	Description: &lt;p&gt;10th percentile of grain size&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983337.rdf
	Name: GrainSizeAvgd50
	Units: micrometers (um)
	Description: &lt;p&gt;50th percentile of grain size&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983338.rdf
	Name: GrainSizeAvgd90
	Units: micrometers (um)
	Description: &lt;p&gt;90th percentile of grain size&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983339.rdf
	Name: GrainSizeMEAN
	Units: micrometers (um)
	Description: &lt;p&gt;mean grain size&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983340.rdf
	Name: GrainSizeSORTING
	Units: unitless
	Description: &lt;p&gt;grain size sorting, which is the standard deviation of the grain size distribution&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983341.rdf
	Name: GrainSizeSKEWNESS
	Units: unitless
	Description: &lt;p&gt;grain size skewness&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983342.rdf
	Name: GrainSizeKURTOSIS
	Units: unitless
	Description: &lt;p&gt;grain size kurtosis&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983343.rdf
	Name: percent_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983344.rdf
	Name: percent_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983345.rdf
	Name: percent_MUD
	Units: percent (%)
	Description: &lt;p&gt;Percent mud&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983346.rdf
	Name: percent_V_COARSE_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent very coarse gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983347.rdf
	Name: percent_COARSE_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent coarse gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983348.rdf
	Name: percent_MEDIUM_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent medium gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983349.rdf
	Name: percent_FINE_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent fine gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983350.rdf
	Name: percent_V_FINE_GRAVEL
	Units: percent (%)
	Description: &lt;p&gt;Percent very fine gravel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983351.rdf
	Name: percent_V_COARSE_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent very coarse sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983352.rdf
	Name: percent_COARSE_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent coarse sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983353.rdf
	Name: percent_MEDIUM_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent medium sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983354.rdf
	Name: percent_FINE_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent fine sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983355.rdf
	Name: percent_V_FINE_SAND
	Units: percent (%)
	Description: &lt;p&gt;Percent very fine sand&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983356.rdf
	Name: percent_V_COARSE_SILT
	Units: percent (%)
	Description: &lt;p&gt;Percent very coarse silt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983357.rdf
	Name: percent_COARSE_SILT
	Units: percent (%)
	Description: &lt;p&gt;Percent coarse silt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983358.rdf
	Name: percent_MEDIUM_SILT
	Units: percent (%)
	Description: &lt;p&gt;Percent medium silt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983359.rdf
	Name: percent_FINE_SILT
	Units: percent (%)
	Description: &lt;p&gt;Percent fine silt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983360.rdf
	Name: percent_V_FINE_SILT
	Units: percent (%)
	Description: &lt;p&gt;Percent very fine silt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983361.rdf
	Name: percent_CLAY
	Units: percent (%)
	Description: &lt;p&gt;Percent clay&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983362.rdf
	Name: SAMPLE_TYPE
	Units: unitless
	Description: &lt;p&gt;grain size sorting&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983363.rdf
	Name: TEXTURAL_GROUP
	Units: unitless
	Description: &lt;p&gt;sediment texture&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/983364.rdf
	Name: SEDIMENT_NAME
	Units: unitless
	Description: &lt;p&gt;type of sediment&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;Sediment cores were collected from 10 meters (m) depth in the northern Gulf of Mexico offshore of Dauphin Island, Alabama, in cohesive muddy sediment on January 26, 2020 (30° 13.333' N, 88° 8.348' W). Cores were collected from a Dauphin Island Sea Lab vessel, the R/V Alabama Discovery. Polycarbonate sediment cores (9.6 centimeter (cm) inner diameter x 60 cm height) were collected using an MC-400 multicorer (Ocean Instruments, Fall City, WA). We resuspended the surface 5 cm of the cores in the lab using a modified baster with holes that directed water jets laterally below the sediment surface. We compared temporal changes in sediment compaction to changes in surface and subsurface cohesion over 30 days post-resuspension.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sediment-water interface (SWI) height and acoustic sound speed through sediment, which depends on bulk density, provided continuous and nondestructive metrics of compaction, and sediment porosity and grain size were measured destructively to characterize sediment physical structure. We determined surface cohesion by measuring both eroded mass and turbidity resulting from increasing shear stress. Subsurface cohesion was determined from the force required for sediments to fail in tension. We compared surface and subsurface exopolymeric substance (EPS) concentrations to surface and subsurface cohesion measurements. We differentiated between water-soluble (colloidal) and sediment-bound EPS as we expected bound EPS to contribute more to sediment-organic matrix development and thus cohesion because they are directly bound to sediment grains rather than dissolved in porewater.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;These data include the summary of data collected on cores processed over time points 0 days (no resuspension), then 1, 2, 3, 7, 14, and 30 days post resuspension. There were 5 replicate cores per time point, but not all data were collected on each replicate core. Detailed data on erosion measurements, as well as repeated non-destructive measurements of sediment-water interface height and sound speed on cores processed on day 30 are provided in separate datasets.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Cores were extruded and sliced at 1 cm depth intervals. We calculated water content from sediment mass differences before and after drying at 65 degrees Celsius (°C) for 24 hours as mass of water divided by mass of dry sediment (Eq. 4.7 from Jackson and Richardson 2007). We measured grain size every cm in the top 5 cm and at 8 and 10 cm for undisturbed cores and cores 3 and 30 days after resuspension. We determined grain size distribution with a Malvern Mastersizer 3000 particle analyzer (Malvern Panalytical, Malvern, UK) and data were analyzed with Gradistat (Kenneth Pye Associates, Ltd., Berkshire, UK) and classified according to Folk and Ward (1957).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We performed acoustic measurements following methods from Dorgan et al. (2020). Within a seawater tank, a 400 kHz three-cycle sinusoidal tone burst was transmitted horizontally through sediment cores to a receiver at 3 depths below the sediment surface (2.5, 5, 10 cm) (see Fig. 1 in Dorgan et al., 2020). To account for sound speed differences due to temporal variability in temperature and salinity, sound speed through sediment was normalized by the sound speed in seawater to obtain sound speed ratio (SSR). Each day, we also performed acoustic measurements on cores filled with seawater and with no core present. Sound speed in seawater and the lag time between the transmitted and received signals (time of flight) through sediment and seawater cores were used to calculate sound speed in sediment (νp):&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;ν_p=c_w/(1-(c_w * ∆t/d_s )&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;where cw is sound speed in water, Δt is the difference in time of flight between seawater core (tw) and sediment core (ts), and ds is the inner diameter of the core (Jackson and Richardson, 2007; Dorgan et al., 2020). SSR was then calculated by dividing νp by cw, where a higher SSR indicates more compact sediment.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To determine if differences in erodibility and tensile strength were driven by variability in surface and subsurface EPS, we analyzed the subcore used for water content and grain size measurements for EPS carbohydrate concentrations. Following methods of de Brouwer and Stal (2001), we lyophilized frozen sediment and extracted colloidal carbohydrates with purified water (E-Pure) for 1 hour at 30 °C. We then extracted bound carbohydrates with 0.1 M Na2EDTA for 16 hours at room temperature. We measured both carbohydrate fractions with the sulfuric acid-UV assay (Albalasmeh et al., 2013), which is based on the phenol-sulfuric acid assay (DuBois et al., 1956). 900 µL 96 % sulfuric acid was added to 300 microliters (µL) carbohydrate solution to dehydrate dissolved carbohydrates into furfural derivatives, which absorb UV light. This solution was vortexed for 30 seconds, allowed to return to room temperature for approximately 5 minutes, then UV absorbance at 315 nanometers (nm) was measured using a SpectraMax M5 microplate reader (Molecular Devices). We determined carbohydrate concentration from UV absorbance of a glucose reference.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To determine subsurface cohesion changes over time, we measured tensile force (N) using a custom probe modified from a fracture toughness probe developed by Johnson et al. (2012). A helical probe is rotated and translated into the sediment like a corkscrew, then pulled upward, breaking off a plug of sediment. Force, measured with an in-line force sensor (Futek LS-200 2-lb), increases to a peak force, then drops when the plug breaks free of the sediment below. Forces from friction with the surrounding sediment and the weight of the sediment plug are removed by repeating the corkscrew motion and subtracting the force profile from the second upward pull. The peak force in the plot of net force as a function of upward distance corresponds to the tensile strength of the sediment, a metric of cohesion. Fracture toughness can be calculated from this peak force (Johnson et al. 2012), but due to some concerns about the effect of sediment depth on these calculations (Dorgan, unpublished data), only force is presented here. These force measurements are comparable across the same depth in different cores, with higher force indicating greater cohesion.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Instruments:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Grain size analysis was done on a Malvern Mastersizer 3000 particle analyzer. Most instruments are custom built. Acoustics measurements were done following Dorgan et al. 2020, JASA. Other measurements are described in Clemo et al., 2022.&amp;lt;/p&amp;gt;</gco:CharacterString>
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Sound speed was calculated from the lag between sent and received 3-pulse sine waves at 400 kHz using a custom Matlab script&amp;amp;nbsp;(see Dorgan et al. 2020 for details). Grain size analysis was done with Gradistat.&amp;lt;/p&amp;gt;</gco:CharacterString>
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