http://lod.bco-dmo.org/id/dataset/876072 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2022-06-21 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Reproductive histology and energetics in Acropora hyacinthus in response to the 2019 Moorea bleaching event. 2022-07-06 publication 2022-07-06 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2022-07-06 publication https://doi.org/10.26008/1912/bco-dmo.876072.1 Marie Strader Auburn University principalInvestigator Gretchen E. Hofmann University of California-Santa Barbara principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Strader, M., Hofmann, G. E. (2022) Reproductive histology and energetics in Acropora hyacinthus in response to the 2019 Moorea bleaching event. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-07-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.876072.1 [access date] Acropora hyacinthus energetics and reproduction Dataset Description: Methods and Sampling: &lt;p&gt;Methodology and analytical procedures documented in Leinbach et al., 2021, but are included here as well:&lt;br /&gt; In tagged coral colonies that were resistant to or recovered from bleaching, the energetic condition of the host was assessed (Nresistant = 12, Nrecovered = 20). One small branch (~ 2–4 centimeters in length) was sampled from each colony and airbrushed in filtered seawater to remove coral tissue and algal cells (blastate) from the skeleton. The blastate was homogenized and 200 μL was collected and preserved in Z-fix (10% zinc formalin) for algal symbiont counts. The remaining blastate was centrifuged at 2000×g for 2 minutes to separate the host tissue from endosymbiont cells. Host tissue slurry was preserved at − 20 °C until further processing. Microalgal endosymbiont density was quantified using a hemocytometer (Hausser Scientific, Horsham, PA) under an Olympus BH-2 microscope. Total host protein content was quantified using a Bradford assay with bovine-serum albumin (BSA) as a standard (Pierce Coomassie Plus Assay Kit, Thermo Fisher Scientific). Total host carbohydrate content was quantified using a modified phenol–sulfuric acid method.&amp;nbsp; All physiological metrics were standardized to coral skeleton surface area following the paraffin wax-dipping technique.&lt;br /&gt; &lt;br /&gt; Small fragments from tagged colonies were sampled by hand via SCUBA (Nresistant = 26, Nrecovered = 21) in October 2019. For each colony, the selected fragment was sampled 5–10 centimeters (cm) from the colony edge, and branch tips and colony edges were avoided. Samples were immediately preserved in Z-fix for 24 hours and then stored in 100% ethanol until histological processing. Samples were decalcified with a 1% EDTA decalcifier solution for 48–72 h and stored in 70% ethanol until processing on a Leica ASP6025 tissue processer. Paraffinized tissue was embedded in wax blocks (Leica EG1150H embedding machine) and then allowed to cool in a freezer 24 hours prior to sectioning. Blocks were serially sectioned at 5 micrometer (μm) thickness on a Leica RM2125RTS microtome every 300 micrometer (μm), which corresponds to the average oocyte diameter. Sections were arranged on microscope slides and stained using a modified Heidenhain’s aniline blue stain on a Leica ST5020 multistainer.&lt;/p&gt; &lt;p&gt;Histological sections were analyzed for measurements of reproductive effort: (1) presence/absence of male and female gametes, (2) diameter of oocytes, and (3) relative fecundity, detailed below. Gametes (oocytes and spermatocytes) were staged from I-V following the classification of Szmant-Froelich et al. Slides were examined using an Olympus BX41 microscope with an Olympus SC180 camera attachment. Measurements were made using ImageJ. Oocyte diameter was determined by averaging the longest and shortest axis of each oocyte. A total of 25 oocytes were measured from each colony. In fragments containing fewer than 25 oocytes, the maximum number of oocytes observed was used (Supplementary Table S1, Leinbach et al., 2021). Only oocytes with a visible nucleus were measured to ensure no oocytes were counted more than once and that the maximum diameter was measured.&lt;/p&gt; &lt;p&gt;Due to the small size of the fragments and polyps, as a proxy for fecundity, three polyps were randomly selected on the middle slide from each individual. When there was an even number of slides, the first of the two middle slides were used. Because only one slide from each individual was examined, there was no risk of double-counting oocytes, so the number of both nucleated and non-nucleated oocytes was counted in each of the randomly selected polyps. These counts were averaged to produce the average number of oocytes per polyp for each individual as a measure of relative fecundity. It should be noted that this relative estimate is lower than true fecundity.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1935308 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935308 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1935305 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935305 completed Marie Strader Auburn University 541 490 9827 Department of Biological Sciences 311 Funchess Hall Auburn AL 368490001 US stradermarie@gmail.com pointOfContact Gretchen E. Hofmann University of California-Santa Barbara 805-893-6175 Department of Ecology, Evolution and Marine Biology, UCSB 4310 Marine Science Institute Santa Barbara CA 93106-9620 USA hofmann@ucsb.edu pointOfContact asNeeded Dataset Version: 1 Unknown coral_ID depth response tagged_month May_status August_status October_status histology_sampled_date energetics_sampled_date oocytes_measured average_relative_fecundity surface_area symbiont_normSA protein_normSA carb_normSA colony_area Olympus SC180 Olympus BH-2 Olympus BX41 Eica ASP6025 theme None, User defined sample identification depth comments month of year date number mean surface_area density protein concentration carbohydrates featureType BCO-DMO Standard Parameters Camera Hemocytometer Microscope - Optical Microscope - Optical Tissue processor instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects https://osprey.bco-dmo.org/project/823206 RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects <p>NSF Award Abstract: </p> <p>Coral reefs provide strong economic and ecological benefits, yet they are declining worldwide largely due to extreme heat events that cause bleaching, a disturbance of the essential relationship between the algae that live inside the coral and the coral. There is currently a mass coral bleaching event in Moorea, French Polynesia where up to 90% of corals show some level of bleaching in response to heat stress. This location is ideal to study adaptation and acclimation thanks to the facilities and sampling of the Moorea Coral Reef (MCR) Long Term Ecological Research (LTER) site. This project explores how strong natural disaster events shape genetic differences in populations through time. By using historical environmental data it may be possible to identify modifications of the genome linked to past bleaching events. This knowledge will help establish models to predict reef recovery after disturbance and will be useful for choosing colonies with the best chance of survival in restoration efforts. This project also investigates how the bleaching history of the parents impacts characteristics of the next generations, such as reproductive output, larval, survival and heat tolerance. This project will provide training and involvement in research for three senior PhD students and at least five undergraduates. Coral restoration efforts rely on understanding how corals might adapt to environmental stress.</p> <p>The mass coral bleaching event currently occurring in French Polynesia (April 2019) offers an opportunity to test hypotheses regarding mechanisms of rapid response to large scale disturbances. This project investigates potential epigenetic and genetic mechanisms involved in either resisting stress or recovering from bleaching. The research leverages the Moorea Coral Reef (MCR) LTER, which integrates the high resolution oceanographic metrics and data on long-term community dynamics into the study of rapid adaptation of Acropora hyacinthus. Genetic and epigenetic signatures of a natural selection event (bleaching) are tracked in the field to test the impact of bleaching history on reproductive and carry-over effects in larval and juvenile corals. Both physiological and molecular methods, such as 2bRAD genotyping and reduced representation bisulfite sequencing, are employed to investigate correlations between phenotypes and genetic and epigenetic differences in the genome. This work explores associations between selection on genetic variation and epigenetic variation as well as the potential role of DNA methylation in phenotypic change across a generation in association with coral bleaching. In this era of global change, there is mounting evidence that rapid evolutionary processes are occurring at time scales relevant to ecological processes. Therefore, capitalizing on a system with rich long-term ecological data, such as that associated with the MCR LTER, is ideal to investigate mechanisms of rapid adaptation.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Moorea coral bleaching largerWorkCitation project eng; USA oceans -149.817 -149.817 -14.4751 -14.4751 2019-10-06 2019-10-15 Moorea, French Polynesia; Auburn University; University of California, Santa Barbara 0 BCO-DMO catalogue of parameters from Reproductive histology and energetics in Acropora hyacinthus in response to the 2019 Moorea bleaching event. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/876652.rdf Name: coral_ID Units: unitless Description: <p>coral identification number</p> http://lod.bco-dmo.org/id/dataset-parameter/876653.rdf Name: depth Units: meters (m) Description: <p>depth of coral sample</p> http://lod.bco-dmo.org/id/dataset-parameter/876654.rdf Name: response Units: unitless Description: <p>response (resistant, bleached, recovered)</p> http://lod.bco-dmo.org/id/dataset-parameter/876655.rdf Name: tagged_month Units: month Description: <p>month of sample tagging</p> http://lod.bco-dmo.org/id/dataset-parameter/876656.rdf Name: May_status Units: unitless Description: <p>status in May</p> http://lod.bco-dmo.org/id/dataset-parameter/876657.rdf Name: August_status Units: unitless Description: <p>status in August</p> http://lod.bco-dmo.org/id/dataset-parameter/876658.rdf Name: October_status Units: unitless Description: <p>status in October</p> http://lod.bco-dmo.org/id/dataset-parameter/876659.rdf Name: histology_sampled_date Units: unitless Description: <p>histology sample date in format YYYY-MM-DD</p> http://lod.bco-dmo.org/id/dataset-parameter/876660.rdf Name: energetics_sampled_date Units: unitless Description: <p>energetics sample date in format YYYY-MM-DD</p> http://lod.bco-dmo.org/id/dataset-parameter/876661.rdf Name: oocytes_measured Units: unitless Description: <p>number of oocytes measured</p> http://lod.bco-dmo.org/id/dataset-parameter/876662.rdf Name: average_relative_fecundity Units: units Description: <p>average relative fecundity</p> http://lod.bco-dmo.org/id/dataset-parameter/876663.rdf Name: surface_area Units: centimeters squared Description: <p>surface area</p> http://lod.bco-dmo.org/id/dataset-parameter/876664.rdf Name: symbiont_normSA Units: symbionts per centimeters squared Description: <p>microalgal endosymbiont density</p> http://lod.bco-dmo.org/id/dataset-parameter/876665.rdf Name: protein_normSA Units: micrograms per centimeters squared Description: <p>total host protein content</p> http://lod.bco-dmo.org/id/dataset-parameter/876666.rdf Name: carb_normSA Units: micrograms per centimeters squared Description: <p>total host carbohydrate content</p> http://lod.bco-dmo.org/id/dataset-parameter/876667.rdf Name: colony_area Units: centimeters squared Description: <p>area of coral colony</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 5913 https://darchive.mblwhoilibrary.org/bitstream/1912/29062/1/dataset-876072_acropora-hyacinthus-energetics-and-reproduction__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.876072.1 download onLine dataset &lt;p&gt;Methodology and analytical procedures documented in Leinbach et al., 2021, but are included here as well:&lt;br /&gt; In tagged coral colonies that were resistant to or recovered from bleaching, the energetic condition of the host was assessed (Nresistant = 12, Nrecovered = 20). One small branch (~ 2–4 centimeters in length) was sampled from each colony and airbrushed in filtered seawater to remove coral tissue and algal cells (blastate) from the skeleton. The blastate was homogenized and 200 μL was collected and preserved in Z-fix (10% zinc formalin) for algal symbiont counts. The remaining blastate was centrifuged at 2000×g for 2 minutes to separate the host tissue from endosymbiont cells. Host tissue slurry was preserved at − 20 °C until further processing. Microalgal endosymbiont density was quantified using a hemocytometer (Hausser Scientific, Horsham, PA) under an Olympus BH-2 microscope. Total host protein content was quantified using a Bradford assay with bovine-serum albumin (BSA) as a standard (Pierce Coomassie Plus Assay Kit, Thermo Fisher Scientific). Total host carbohydrate content was quantified using a modified phenol–sulfuric acid method.&amp;nbsp; All physiological metrics were standardized to coral skeleton surface area following the paraffin wax-dipping technique.&lt;br /&gt; &lt;br /&gt; Small fragments from tagged colonies were sampled by hand via SCUBA (Nresistant = 26, Nrecovered = 21) in October 2019. For each colony, the selected fragment was sampled 5–10 centimeters (cm) from the colony edge, and branch tips and colony edges were avoided. Samples were immediately preserved in Z-fix for 24 hours and then stored in 100% ethanol until histological processing. Samples were decalcified with a 1% EDTA decalcifier solution for 48–72 h and stored in 70% ethanol until processing on a Leica ASP6025 tissue processer. Paraffinized tissue was embedded in wax blocks (Leica EG1150H embedding machine) and then allowed to cool in a freezer 24 hours prior to sectioning. Blocks were serially sectioned at 5 micrometer (μm) thickness on a Leica RM2125RTS microtome every 300 micrometer (μm), which corresponds to the average oocyte diameter. Sections were arranged on microscope slides and stained using a modified Heidenhain’s aniline blue stain on a Leica ST5020 multistainer.&lt;/p&gt; &lt;p&gt;Histological sections were analyzed for measurements of reproductive effort: (1) presence/absence of male and female gametes, (2) diameter of oocytes, and (3) relative fecundity, detailed below. Gametes (oocytes and spermatocytes) were staged from I-V following the classification of Szmant-Froelich et al. Slides were examined using an Olympus BX41 microscope with an Olympus SC180 camera attachment. Measurements were made using ImageJ. Oocyte diameter was determined by averaging the longest and shortest axis of each oocyte. A total of 25 oocytes were measured from each colony. In fragments containing fewer than 25 oocytes, the maximum number of oocytes observed was used (Supplementary Table S1, Leinbach et al., 2021). Only oocytes with a visible nucleus were measured to ensure no oocytes were counted more than once and that the maximum diameter was measured.&lt;/p&gt; &lt;p&gt;Due to the small size of the fragments and polyps, as a proxy for fecundity, three polyps were randomly selected on the middle slide from each individual. When there was an even number of slides, the first of the two middle slides were used. Because only one slide from each individual was examined, there was no risk of double-counting oocytes, so the number of both nucleated and non-nucleated oocytes was counted in each of the randomly selected polyps. These counts were averaged to produce the average number of oocytes per polyp for each individual as a measure of relative fecundity. It should be noted that this relative estimate is lower than true fecundity.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;BCO-DMO Processing description:&lt;/strong&gt;&lt;br /&gt; - Adjusted field/parameter names to comply with BCO-DMO naming conventions&lt;br /&gt; - Missing data identifier ‘NA’ replaced with 'nd' (BCO-DMO's default missing data identifier)&amp;nbsp;&lt;br /&gt; - Added a conventional header with dataset name, PI names, version date&lt;br /&gt; - Rounded columns to 3 decimal places&lt;br /&gt; &lt;br /&gt; &amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Olympus SC180 Olympus SC180 PI Supplied Instrument Name: Olympus SC180 Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/ PI Supplied Instrument Name: Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html. Olympus BH-2 Olympus BH-2 PI Supplied Instrument Name: Olympus BH-2 Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/ Olympus BX41 Olympus BX41 PI Supplied Instrument Name: Olympus BX41 PI Supplied Instrument Description:used with Olympus SC180 camera attachment Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/ Eica ASP6025 Eica ASP6025 PI Supplied Instrument Name: Eica ASP6025 Instrument Name: Tissue processor Instrument Short Name: Instrument Description: An automated system used to process tissue specimens for examination through fixation, dehydration, and infiltration.