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&amp;lt;p&amp;gt;** Need additional details from the PI **&amp;lt;/p&amp;gt;</gco:CharacterString>
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This is a focused program of field research in waters of the Western Tropical North Atlantic influenced by the Amazon River Plume during the high river flow season. The Amazon Plume region supports diverse plankton communities in a dynamic system driven by nutrients supplied by transport from the river proper as well as nutrients entrained from offshore waters by physical mixing and upwelling. This creates strong interactions among physical, chemical, and biological processes across a range of spatial and temporal scales. The field program will link direct measurements of environmental properties with focused experimental studies of nutrient supply and nutrient limitation of phytoplankton, as well as the transfer of phytoplankton nitrogen to the zooplankton food web. The Amazon Plume exhibits a close juxtaposition of distinct communities during the high-flow season, making it an ideal site for evaluating how nutrient availability, nutrient supply, and habitat longevity interact to drive offshore ecosystem dynamics and function. This project will include German collaborators and will seamlessly integrate education and research efforts. The investigators and their institutions have a strong commitment to undergraduate and graduate education and to increasing the diversity of the ocean science community through active recruiting and training efforts. The team has a strong track record of involving both undergraduate and graduate students in their field and lab research. The two research cruises planned will provide opportunities for students and technicians to interact with an interdisciplinary and international research team.&lt;/p&gt;
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&lt;p&gt;This research will study the processes that cause the relative biological availability of nitrogen (N) and phosphorus (P) in the upper ocean to change as water moves from the coast to offshore in the northern Gulf of Mexico. Both N and P are required nutrients. Consequently, the ratio of N to P in the upper ocean has important consequences for plant growth and the marine food web structure. Typically, as water moves offshore bioavailable N declines faster than bioavailable P. While processes that alter either element will affect the N to P ratio, previous research has focused mainly on the N side of the relationship, examining cycling and the selective removal of different forms of N from the water by marine microorganisms. This project will focus instead on the less-studied P side of the N to P ratio in the upper ocean. It will use shipboard experiments to quantify microbiological processes that maintain P availability in the upper ocean, even as N availability declines. Given that low N availability relative to P limits plant growth in most of the ocean's sunlit surface waters, understanding how this chemical ratio develops as water moves offshore is of fundamental importance for the study of marine ecosystems worldwide. Educational impact will include at least seven students' direct participation in the research, providing hands-on and cross-disciplinary training, as well as practical experience at sea. Two middle school teachers will also participate in the oceanographic cruises. They will incorporate field results and personal experiences into lesson plans and teachers' workshops. The project will also develop public outreach activities that focus on the unique value of marine ecosystems of the Gulf of Mexico.&lt;/p&gt;
&lt;p&gt;This project will test the hypothesis that the decline in the ratio of bioavailable N to P in surface water as it moves offshore develops from preferential phosphorus retention as opposed to removal of biologically-available forms of nitrogen. As part of the research associated with this central hypothesis, the project will quantitatively compare the relative importance of different phosphorus-retention mechanisms during two oceanographic cruises in the northern Gulf of Mexico. Previous observations of spatial changes in N and P availability are common. The researchers will track discrete water masses with Lagrangian drifters for time course sampling, and use physical oceanographic measurements to quantify potential N to P ratio changes contributed by vertical and horizontal mixing. Shipboard incubation experiments will quantify and compare rates for the key microbiological processes thought to affect phosphorus retention in the upper ocean. This focus on potential P-retention processes rather than N loss as an explanation of commonly observed declines in surface ocean N to P ratio represents a unique contribution to the complete understanding of the complex feedback mechanisms between nutrient cycles and marine ecosystem function.&lt;/p&gt;</gco:CharacterString>
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	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876274.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876275.rdf
	Name: Cruise
	Units: unitless
	Description: &lt;p&gt;Cruise Name&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876277.rdf
	Name: Date
	Units: unitless
	Description: &lt;p&gt;Date UTC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876278.rdf
	Name: Station_Event
	Units: unitless
	Description: &lt;p&gt;Station Event&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876279.rdf
	Name: Bottle
	Units: unitless
	Description: &lt;p&gt;CTD Bottle (24 bottles total)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876280.rdf
	Name: DepSM
	Units: meters (m)
	Description: &lt;p&gt;Depth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876281.rdf
	Name: Fcom_to_R_B
	Units: units?
	Description: &lt;p&gt;CDOM measured by blue laser normalized to Raman scattering&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876282.rdf
	Name: Chl_G
	Units: microgram per liter (ug/L)
	Description: &lt;p&gt;Chlorophyll a concentration from green spectra&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876283.rdf
	Name: Fchl_to_R_G
	Units: units?
	Description: &lt;p&gt;Chlorophyll fluorescence measured by the green laser normalized to Raman scattering&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876284.rdf
	Name: Fpe1_to_R
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (565 nm) normalized to Raman&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876285.rdf
	Name: Fpe2_to_R
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (575 nm) normalized to Raman&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876286.rdf
	Name: Fpe3_to_R
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (589 nm) normalized to Raman&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876287.rdf
	Name: Fpe1_to_Fchl
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (565 nm) normalized to max chlorophyll fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876288.rdf
	Name: Fpe2_to_Fchl
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (575 nm) normalized to max chlorophyll fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876289.rdf
	Name: Fpe3_to_Fchl
	Units: units?
	Description: &lt;p&gt;Phycoerythrin fluorescence (589 nm) normalized to max chlorophyll fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876290.rdf
	Name: Fpe12_to_R
	Units: units?
	Description: &lt;p&gt;Sum of the max of Phycoerythrin flourescence measurements 1 and 2 (565 nm and 575 nm) normalized to Raman&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876291.rdf
	Name: Fpe12_to_Fchl
	Units: units?
	Description: &lt;p&gt;Sum of the max of Phycoerythrin flourescence measurements 1 and 2 (565 nm and 575 nm) normalized to max chlorophyll fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876292.rdf
	Name: FvFm_G
	Units: units?
	Description: &lt;p&gt;Variable Fluorescence Green Laser&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876293.rdf
	Name: FvFm_Gc
	Units: units?
	Description: &lt;p&gt;Variable Fluorescence Green Laser corrected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876294.rdf
	Name: Sal00
	Units: practical salinity unit (PSU)
	Description: &lt;p&gt;Practical Salinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876295.rdf
	Name: Sigma_t00
	Units: kilogram per cubic meter (kg/m^3)
	Description: &lt;p&gt;Density, Sigma-theta&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876296.rdf
	Name: OxsatMm_Kg
	Units: micromole per kilogram (umol/kg)
	Description: &lt;p&gt;Oxygen Saturation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876297.rdf
	Name: Sbeox0MmL
	Units: micromole per liter (umol/L)
	Description: &lt;p&gt;Oxygen from SBE 43 primary sensor, WS = 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876298.rdf
	Name: Sbeox1MmL
	Units: micromole per liter (umol/L)
	Description: &lt;p&gt;Oxygen from SBE 43 secondary sensor, WS = 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876299.rdf
	Name: Potemp090C
	Units: degrees Celsius
	Description: &lt;p&gt;Potential Temperature (ITS-90)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876300.rdf
	Name: SvCM
	Units: meters per second (m/s)
	Description: &lt;p&gt;Sound Velocity after Chen-Millero&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876301.rdf
	Name: Scan
	Units: unitless
	Description: &lt;p&gt;Scan Count&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876302.rdf
	Name: TimeJ
	Units: unitless
	Description: &lt;p&gt;Julian Days&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876303.rdf
	Name: TimeS
	Units: seconds
	Description: &lt;p&gt;Elapsed Time&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876304.rdf
	Name: PrDM
	Units: decibars (db)
	Description: &lt;p&gt;Pressure from Digiquartz sensor&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876305.rdf
	Name: T090C
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature (ITS-90)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876306.rdf
	Name: C0Sm
	Units: Siemens per meter (S/m)
	Description: &lt;p&gt;Conductivity (c0S/m)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876307.rdf
	Name: CStarAt0
	Units: per meter (1/m)
	Description: &lt;p&gt;Beam Attenuation from WET Labs C-Star&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876308.rdf
	Name: CStarTr0
	Units: percent (%)
	Description: &lt;p&gt;Beam Transmission from WET Labs C-Star&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876309.rdf
	Name: FlECO_AFL
	Units: milligram per cubic meter (mg/m^3)
	Description: &lt;p&gt;Fluorescence from WET Labs ECO-AFL/FL&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876310.rdf
	Name: AltM
	Units: meters
	Description: &lt;p&gt;Altimeter (altM) reading&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876311.rdf
	Name: PAR
	Units: micromole photos per square meters per second (umol photons/m^2/sec)
	Description: &lt;p&gt;Irradiance (PAR) from Biospherical/Licor&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876312.rdf
	Name: Sbeox0V
	Units: volts (V)
	Description: &lt;p&gt;Oxygen raw from SBE 43 first voltage sensor&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876313.rdf
	Name: Sbeox1V
	Units: volts (V)
	Description: &lt;p&gt;Oxygen raw from SBE 43 second voltage sensor&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/876314.rdf
	Name: SPAR
	Units: micromole photos per square meters per second (umol photons/m^2/sec)
	Description: &lt;p&gt;Surface Irradiance (SPAR) from Biospherical/Licor&lt;/p&gt; 
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