http://lod.bco-dmo.org/id/dataset/876564
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-06-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019
2022-06-29
publication
2022-06-29
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-08-09
publication
https://doi.org/10.26008/1912/bco-dmo.876564.1
Marie Strader
Auburn University
principalInvestigator
Gretchen E. Hofmann
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Strader, M., Hofmann, G. E. (2022) ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-06-29 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.876564.1 [access date]
Methods and Sampling: <p>To assess in hospite symbiont community dynamics in a major reef-building coral, <em>Acropora hyacinthus</em>, during a mass bleaching event we sampled small pieces of tissue from each colony and performed ITS2 amplicon sequencing. Samples were collected at the height of the bleaching event (May 2019), 3 months after (August 2019) and 5 months later (October 2019). Samples were collected at 3 different reef zones, backreef (~1 meter depth), shallow forereef (~5 meter depth), and deeper forereef (~14 meter depth).<br />
<br />
Raw sequence data have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject number PRJNA833391. This dataset also includes relevant metadata for each sample.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1935308 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935308
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1935305 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1935305
completed
Marie Strader
Auburn University
541 490 9827
Department of Biological Sciences 311 Funchess Hall
Auburn
AL
368490001
US
stradermarie@gmail.com
pointOfContact
Gretchen E. Hofmann
University of California-Santa Barbara
805-893-6175
Department of Ecology, Evolution and Marine Biology, UCSB 4310 Marine Science Institute
Santa Barbara
CA
93106-9620
USA
hofmann@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Accession
Sample_Name
BioSample
Details
Host
Location
Date
Depth
Latitude
Longitude
theme
None, User defined
accession number
sample identification
comments
species scientific name (binomial)
site
date
depth
latitude
longitude
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects
https://osprey.bco-dmo.org/project/823206
RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects
<p>NSF Award Abstract: </p>
<p>Coral reefs provide strong economic and ecological benefits, yet they are declining worldwide largely due to extreme heat events that cause bleaching, a disturbance of the essential relationship between the algae that live inside the coral and the coral. There is currently a mass coral bleaching event in Moorea, French Polynesia where up to 90% of corals show some level of bleaching in response to heat stress. This location is ideal to study adaptation and acclimation thanks to the facilities and sampling of the Moorea Coral Reef (MCR) Long Term Ecological Research (LTER) site. This project explores how strong natural disaster events shape genetic differences in populations through time. By using historical environmental data it may be possible to identify modifications of the genome linked to past bleaching events. This knowledge will help establish models to predict reef recovery after disturbance and will be useful for choosing colonies with the best chance of survival in restoration efforts. This project also investigates how the bleaching history of the parents impacts characteristics of the next generations, such as reproductive output, larval, survival and heat tolerance. This project will provide training and involvement in research for three senior PhD students and at least five undergraduates. Coral restoration efforts rely on understanding how corals might adapt to environmental stress.</p>
<p>The mass coral bleaching event currently occurring in French Polynesia (April 2019) offers an opportunity to test hypotheses regarding mechanisms of rapid response to large scale disturbances. This project investigates potential epigenetic and genetic mechanisms involved in either resisting stress or recovering from bleaching. The research leverages the Moorea Coral Reef (MCR) LTER, which integrates the high resolution oceanographic metrics and data on long-term community dynamics into the study of rapid adaptation of Acropora hyacinthus. Genetic and epigenetic signatures of a natural selection event (bleaching) are tracked in the field to test the impact of bleaching history on reproductive and carry-over effects in larval and juvenile corals. Both physiological and molecular methods, such as 2bRAD genotyping and reduced representation bisulfite sequencing, are employed to investigate correlations between phenotypes and genetic and epigenetic differences in the genome. This work explores associations between selection on genetic variation and epigenetic variation as well as the potential role of DNA methylation in phenotypic change across a generation in association with coral bleaching. In this era of global change, there is mounting evidence that rapid evolutionary processes are occurring at time scales relevant to ecological processes. Therefore, capitalizing on a system with rich long-term ecological data, such as that associated with the MCR LTER, is ideal to investigate mechanisms of rapid adaptation.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Moorea coral bleaching
largerWorkCitation
project
eng; USA
oceans
-149.818
-149.817
-17.4731
-14.4751
2019-05-19
2019-10-09
Moorea, French Polynesia; Auburn University; University of California, Santa Barbara
0
BCO-DMO catalogue of parameters from ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/876580.rdf
Name: Accession
Units: unitless
Description: <p>NCBI accession number</p>
http://lod.bco-dmo.org/id/dataset-parameter/876581.rdf
Name: Sample_Name
Units: unitless
Description: <p>name of individual sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/876582.rdf
Name: BioSample
Units: unitless
Description: <p>NCBI BioSample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/876583.rdf
Name: Details
Units: unitless
Description: <p>description of colony and location of coral reef</p>
http://lod.bco-dmo.org/id/dataset-parameter/876584.rdf
Name: Host
Units: unitless
Description: <p>species identification of coral host</p>
http://lod.bco-dmo.org/id/dataset-parameter/876585.rdf
Name: Location
Units: unitless
Description: <p>location of sample site</p>
http://lod.bco-dmo.org/id/dataset-parameter/876586.rdf
Name: Date
Units: unitless
Description: <p>date of sample collection in format YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/876587.rdf
Name: Depth
Units: meters (m)
Description: <p>water depth of sample site</p>
http://lod.bco-dmo.org/id/dataset-parameter/876588.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude North of sample site</p>
http://lod.bco-dmo.org/id/dataset-parameter/876589.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude East of sample site</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
28350
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/756a9b40-b2e1-5989-8a99-26ec297c74ed/content
download
https://doi.org/10.26008/1912/bco-dmo.876564.1
download
onLine
dataset
<p>To assess in hospite symbiont community dynamics in a major reef-building coral, <em>Acropora hyacinthus</em>, during a mass bleaching event we sampled small pieces of tissue from each colony and performed ITS2 amplicon sequencing. Samples were collected at the height of the bleaching event (May 2019), 3 months after (August 2019) and 5 months later (October 2019). Samples were collected at 3 different reef zones, backreef (~1 meter depth), shallow forereef (~5 meter depth), and deeper forereef (~14 meter depth).<br />
<br />
Raw sequence data have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject number PRJNA833391. This dataset also includes relevant metadata for each sample.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Description:</strong><br />
- Converted date columns to format YYYY-MM-DD<br />
- Adjusted field/parameter names to comply with BCO-DMO naming conventions<br />
- Converted latitude and longitude from South and West to North and East and split columns<br />
- Added a conventional header with dataset name, PI names, version date</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact