http://lod.bco-dmo.org/id/dataset/876590
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-07-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Biological, physical, and chemical data from surface Transect 5 on MV1405 (IRN-BRU)
2022-07-06
publication
2022-07-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-07-06
publication
https://doi.org/10.26008/1912/bco-dmo.876590.1
Phoebe Dreux Chappell
Old Dominion University
principalInvestigator
Kenneth W. Bruland
University of California-Santa Cruz
principalInvestigator
Tyler Coale
J. Craig Venter Institute
principalInvestigator
Claire P. Till
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Chappell, P. D., Till, C. P., Coale, T., Bruland, K. W. (2022) Biological, physical, and chemical data from surface Transect 5 on MV1405 (IRN-BRU). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-07-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.876590.1 [access date]
Dataset Description: <p>The complete dataset for the ships underway system includes the following parameters: BOTTOM_DEPTH, TRUE_WIND_SPEED, BAROM_PRES, PAR, CORR_SST, CORR_SALINITY, FLUORESCENCE. This dataset is available via the Rolling Deck to&nbsp; Repository: rvdata.us/search/cruise/MV1405</p>
<p>Diatom 18S Amplicon Sequencing from California Current System Mesoscale Eddies. Available from: NCBI:BioProject: PRJNA743307</p>
<p>OCE-1259776 is credited since it generated the metal and nutrient data that are part of this dataset.</p> Methods and Sampling: <p>Sampling location: California Current System 38 N -126 E depth of sampling ~2m</p>
<p>Nitrate-nitrite, Phosphate, Silicate: Samples were collected from a trace-metal clean towed-fish system (Bruland et al 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak filter capsule (Pall 500) and analyzed shortly after collection at sea using standard spectrophotometric methods (Parsons 1984) on a Lachat QuickChem 8000 Flow Injection Analysis System.</p>
<p>Dissolved iron and manganese samples were collected from a trace-metal clean towed-fish&nbsp;system (Bruland et al., 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak Supor 200 filter capsule (Pall), acidified at sea to pH 1.7-1.8 with quartz-distilled HCl and stored at room temperature until analysis. Once in the lab, samples were buffered to pH 6.0 +/- 0.2 with ammonium acetate and immediately pre-concentrated using Nobias-chelate PA1 chelating resin (concentration factor ~24). Extracts were analyzed on an Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer (HR ICP-MS)&nbsp;using techniques described initially in Biller and Bruland (2012) with adaptations listed in Parker et al. (2016).</p>
<p>Diatom Amplicon Sequencing: Whole seawater was collected from a trace-metal clean towed-fish system (Bruland et al 2005) and filtered onto 3 um polyester filters using a peristaltic pump system. Filters were preserved in RLTPlus buffer and immediately frozen in liquid nitrogen.&nbsp; For long term storage samples were maintained at -80 degrees celsius until extraction.&nbsp; DNA extraction, PCR amplification, and analysis followed that of Chappell et al. 2016 with modification listed in Oliver et al. 2021. Briefly, DNA and RNA were co-extracted using a Qiagen Allprep RNA/DNA mini kit. Diatom 18S was amplified from the DNA using diatom-specific primers modified for Illumina indexing.&nbsp; PCR products were purified, multiplexed using Illumina indices, and sequenced on an Illumina MiSeq desktop sequencer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1524482 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1524482
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1259776 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1259776
completed
Phoebe Dreux Chappell
Old Dominion University
USF St. Petersburg campus 140 7th Ave. South
St. Petersburg
FL
33701
USA
dreux@usf.edu
pointOfContact
Kenneth W. Bruland
University of California-Santa Cruz
(831) 459-4587
Ocean Sciences Department University of California 1156 High Street
Santa Cruz
CA
95064
USA
bruland@ucsc.edu
pointOfContact
Tyler Coale
J. Craig Venter Institute
Tcoale@jcvi.org
pointOfContact
Claire P. Till
University of California-Santa Cruz
707-826-3266
Chemistry Department 1 Harpst Street
Arcata
CA
95521
USA
claire.till@humboldt.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
DATE_TIME
LATITUDE
LONGITUDE
T5_STN_NO
BOTTOM_DEPTH
TRUE_WIND_SPEED
BAROM_PRES
PAR
CORR_SST
CORR_SALINITY
FLUORESCENCE
CORR_NO3
CORR_PO4
CORR_SiO4
DFe
DMn
BIOPROJECT_ACCESSION
BIOSAMPLE_ACCESSION
ISO_DateTime_UTC
WindmasterPro Anemometer (Gill Instruments)
Illumina MiSeq desktop sequencer
Lachat QuickChem 8000 Flow Injection Analysis System
ThermoFisher Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer
Sea-Bird SBE-45
Life Technologies SimpliAmp Thermal Cycler
Turner 10AU fluorometer
theme
None, User defined
Date and time
latitude
longitude
station
depth
wind_speed
barometric pressure
PAR
sea surface temperature
No BCO-DMO term
fluorescence
nitrate plus nitrite
reactive phosphorus (PO4)
Silicate (reactive silica)
Iron
Manganese
NCBI BioProject accession
NCBI BioSample accession
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Anemometer
Automated DNA Sequencer
Flow Injection Analyzer
Inductively Coupled Plasma Mass Spectrometer
Sea-Bird SBE 45 MicroTSG Thermosalinograph
Thermal Cycler
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
MV1405
service
Deployment Activity
California Coastline
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
OCE-RIG: Developing Molecular Bioassays for Evaluating Iron Status of Environmentally Relevant Diatoms
https://osprey.bco-dmo.org/project/713844
OCE-RIG: Developing Molecular Bioassays for Evaluating Iron Status of Environmentally Relevant Diatoms
<p>This project focuses on an important group of photosynthetic algae, the diatoms, and how iron availability modulates their growth. Diatoms are important organisms at the base of the marine food web. Iron is required for the structure and function of proteins essential to diatom growth. Iron concentrations can be low in many areas of the ocean and exists in different forms classified by size and chemical characteristics. Recent efforts have increased understanding of the distribution of these different iron forms, but which forms individual phytoplankton can access remains unclear. The investigator will conduct experiments with important diatom species to develop and use assays that can assess whether iron provided in different forms or from different sources can be accessed by those species to alleviate iron stress. These data will help us understand the relationship between iron and an important group of organisms at the base of the food web, which will prove valuable to climate and food web modelers. This project will further the NSF goals of training new generations of scientists and making scientific discoveries available to the general public. The project supports the research of an early-career scientist, the training of undergraduate and high school students, including inner city middle and high school girls.</p>
<p>The project combines trace metal biogeochemistry, phytoplankton cultivation, and molecular biology to address questions about the bioavailability of various iron forms to environmentally relevant diatoms. Iron is an essential micronutrient for marine phytoplankton. While knowledge of the distribution of iron has increased significantly as a result of the ongoing GEOTRACES program, a mechanistic and quantitative understanding of the bioavailability of various forms of iron remains illusive. Previously, the investigator developed a calibrated molecular bioassay capable of indicating when a specific oceanic diatom was experiencing iron stress. It has been used to evaluate iron stress in oceanic field samples and in incubation samples in response to additions of different iron fractions. The goal of this proposal is to develop additional calibrated molecular assays for iron stress in other environmentally relevant diatoms from both coastal and oceanic habitats and use them to asses the biological availability of iron from varying sources. The experimental plan includes culture experiments monitoring growth, photosynthetic physiology, and gene expression of iron stress response genes. The molecular assays will then be used to test whether iron from a variety of sources can alleviate iron stress in the different species. Results from this project will provide tools to answer fundamental questions about the bioavailability of various forms of iron to important phytoplankton. As such, this research will provide valuable information for biogeochemical modelers, increasing their ability to use the data generated from the GEOTRACES program to accurately predict future changes in ocean productivity.</p>
DiatomFeMolBio
largerWorkCitation
project
Accomplishment Based Renewal: An iron limitation mosaic within the central California Current System
https://osprey.bco-dmo.org/project/876635
Accomplishment Based Renewal: An iron limitation mosaic within the central California Current System
<p><em>NSF Award Abstract:</em><br />
Eastern boundary upwelling systems have long been recognized for their high phytoplankton productivity. Carr and Kearns (2003), in a detailed comparison of eastern boundary current systems, reported that biomass sustained by a given macronutrient concentration in Atlantic eastern boundary current systems was twice as large as those systems in the Pacific. The authors concluded "It is not clear whether the apparent difference in biomass supported by available nutrients is due to differences in the efficiency of the phytoplankton community, perhaps related to the availability of iron, or to grazing pressure." They suggested that the width of the shelf might be considered a proxy for the benthic availability of iron. The lowest biomass for a given macronutrient concentration was in the Peru-Humboldt Current and in the northern California region of the California Current System, both areas with low dust inputs and a relatively narrow shelf.</p>
<p>In this Accomplishment Based Renewal project, a marine trace metal geochemist at the University of California - Santa Cruz and his students and colleagues will continue a decades-old quest to understand the role of iron in the central California Current System (cCCS). Field efforts will combine continuous underway iron and nutrient data in surface waters and a series of vertical profiles. The focus will include three regions within the cCCS: a variety of active Fe-replete and Fe-deplete coastal upwelling regimes, the eddy-rich California Current transition zone that is Fe-limited and has elevated nitrate but relatively low and uniform chlorophyll concentrations, and the offshore, oligotrophic California Current. They will map surface and depth distributions of Fe and other micro- and macronutrients. There are four specific goals dealing with characterizing the organic Fe(III)-binding organic ligands, determining Fe(II) and Fe(III) concentrations in hypoxic waters over the shelf, examining the exchange between particulate and dissolved forms of Fe, and studying the roles of eddies in the eddy-rich transition waters of the cCCS.</p>
<p>Broader Impacts</p>
<p>Direct Benefits to Science: There is a great deal of interest in the CCS because of its importance in terms of phytoplankton productivity and the support of higher trophic levels. Until now, the emphasis in studies of the CCS has been on relationships between physics and biology. This study will insert the important role of micronutrient chemistry into the picture. It will also serve an important role in securing ship time in advance and providing logistical support for other collaborative studies. This is extremely valuable and cost effective for collaborating scientists since with the hydrography, nutrient and trace metal data provided, they can focus on their complimentary research efforts.</p>
<p>Outreach and Education: The project will provide funding for two current graduate students at UCSC, where they will also receive course training in a curriculum that includes i) scientific communication, ii) careers in marine science, and iii) grant writing. A broader impact goal of this project is to facilitate teaching and learning on marine science-related topics through translating research objectives into widely distributed educational materials for classroom use. To accomplish this, the team will partner with the Seymour Discovery Center at the Long Marine Lab, UCSC. The Discovery Center receives 14,000 visitors each year, and the project will provide funds to develop an interactive display on limiting nutrients and phytoplankton bloom development in the CCS.</p>
iron limitation mosaic
largerWorkCitation
project
eng; USA
oceans
California Coastline
-127.317
-124.412
37.5083
39.4836
2014-07-13
2014-07-14
0
BCO-DMO catalogue of parameters from Biological, physical, and chemical data from surface Transect 5 on MV1405 (IRN-BRU)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/876614.rdf
Name: DATE_TIME
Units: unitless
Description: <p>GMT date and time where samples were collected from underway system in MM/DD/YY HH:MM format</p>
http://lod.bco-dmo.org/id/dataset-parameter/876615.rdf
Name: LATITUDE
Units: decimal degrees
Description: <p>Position of ship when samples were collected from underway system in decimal degrees N</p>
http://lod.bco-dmo.org/id/dataset-parameter/876616.rdf
Name: LONGITUDE
Units: decimal degrees
Description: <p>Position of ship when samples were collected from underway system in decimal degrees E</p>
http://lod.bco-dmo.org/id/dataset-parameter/876617.rdf
Name: T5_STN_NO
Units: unitless
Description: <p>Station number given to the sample collection along the T5 transect</p>
http://lod.bco-dmo.org/id/dataset-parameter/876618.rdf
Name: BOTTOM_DEPTH
Units: meters (m)
Description: <p>Depth of the water column</p>
http://lod.bco-dmo.org/id/dataset-parameter/876619.rdf
Name: TRUE_WIND_SPEED
Units: meters (m) per second (s)
Description: <p>Wind speed</p>
http://lod.bco-dmo.org/id/dataset-parameter/876620.rdf
Name: BAROM_PRES
Units: millibars (mb)
Description: <p>Barometric Pressure</p>
http://lod.bco-dmo.org/id/dataset-parameter/876621.rdf
Name: PAR
Units: uE/second/meter^2
Description: <p>surface photoactive radiation</p>
http://lod.bco-dmo.org/id/dataset-parameter/876622.rdf
Name: CORR_SST
Units: degrees celcius
Description: <p>Sea surface temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/876623.rdf
Name: CORR_SALINITY
Units: unitless
Description: <p>salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/876624.rdf
Name: FLUORESCENCE
Units: ug/l
Description: <p>chlorphyll fluroescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/876625.rdf
Name: CORR_NO3
Units: micromoles per liter (uM)
Description: <p>concentration of dissolved nitrate+nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/876626.rdf
Name: CORR_PO4
Units: micromoles per liter (uM)
Description: <p>concentration of dissolved phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/876627.rdf
Name: CORR_SiO4
Units: micromoles per liter (uM)
Description: <p>concentration of dissolved silicic acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/876628.rdf
Name: DFe
Units: nanomoles per kg seawater (nmol/kg)
Description: <p>concentration of dissolved iron. Noted as na when no data available for this sample, no sample analyzed or obviously erroneous data value</p>
http://lod.bco-dmo.org/id/dataset-parameter/876629.rdf
Name: DMn
Units: nanomoles per kg seawater (nmol/kg)
Description: <p>concentration of dissolved manganese. Noted as na when no data available for this sample, no sample analyzed or obviously erroneous data value</p>
http://lod.bco-dmo.org/id/dataset-parameter/876630.rdf
Name: BIOPROJECT_ACCESSION
Units: unitless
Description: <p>NCBI SRA Bioproject accession number for diatom 18S amplicon sequencing.</p>
http://lod.bco-dmo.org/id/dataset-parameter/876631.rdf
Name: BIOSAMPLE_ACCESSION
Units: unitless
Description: <p>NCBI SRA Biosample accession number for diatom 18S amplicon sequencing.</p>
http://lod.bco-dmo.org/id/dataset-parameter/876632.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time (UTC) where samples were collected from underway system in ISO format (yyyy-mm-ddThh:mmZ)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3297
https://darchive.mblwhoilibrary.org/bitstream/1912/29061/1/dataset-876590_irn-bru-mv1405-transect-5-biogeochem__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.876590.1
download
onLine
dataset
<p>Sampling location: California Current System 38 N -126 E depth of sampling ~2m</p>
<p>Nitrate-nitrite, Phosphate, Silicate: Samples were collected from a trace-metal clean towed-fish system (Bruland et al 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak filter capsule (Pall 500) and analyzed shortly after collection at sea using standard spectrophotometric methods (Parsons 1984) on a Lachat QuickChem 8000 Flow Injection Analysis System.</p>
<p>Dissolved iron and manganese samples were collected from a trace-metal clean towed-fish&nbsp;system (Bruland et al., 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak Supor 200 filter capsule (Pall), acidified at sea to pH 1.7-1.8 with quartz-distilled HCl and stored at room temperature until analysis. Once in the lab, samples were buffered to pH 6.0 +/- 0.2 with ammonium acetate and immediately pre-concentrated using Nobias-chelate PA1 chelating resin (concentration factor ~24). Extracts were analyzed on an Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer (HR ICP-MS)&nbsp;using techniques described initially in Biller and Bruland (2012) with adaptations listed in Parker et al. (2016).</p>
<p>Diatom Amplicon Sequencing: Whole seawater was collected from a trace-metal clean towed-fish system (Bruland et al 2005) and filtered onto 3 um polyester filters using a peristaltic pump system. Filters were preserved in RLTPlus buffer and immediately frozen in liquid nitrogen.&nbsp; For long term storage samples were maintained at -80 degrees celsius until extraction.&nbsp; DNA extraction, PCR amplification, and analysis followed that of Chappell et al. 2016 with modification listed in Oliver et al. 2021. Briefly, DNA and RNA were co-extracted using a Qiagen Allprep RNA/DNA mini kit. Diatom 18S was amplified from the DNA using diatom-specific primers modified for Illumina indexing.&nbsp; PCR products were purified, multiplexed using Illumina indices, and sequenced on an Illumina MiSeq desktop sequencer.</p>
Specified by the Principal Investigator(s)
<p>Processing of original SBE-45 data included interpolation between data points and identification of data where flow rate for sensors dropped below 1L/min (indicative of a filter clog or shipboard maintenance).</p>
<p>BCO-DMO Processing Notes:<br />
* Added ISO formatted DateTime column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
WindmasterPro Anemometer (Gill Instruments)
WindmasterPro Anemometer (Gill Instruments)
PI Supplied Instrument Name: WindmasterPro Anemometer (Gill Instruments) PI Supplied Instrument Description:WindmasterPro Anemometer (Gill Instruments) Instrument Name: Anemometer Instrument Short Name:Anemometer Instrument Description: An anemometer is a device for measuring the velocity or the pressure of the wind. It is commonly used to measure wind speed. Aboard research vessels, it is often mounted with other meteorological instruments and sensors. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/101/
Illumina MiSeq desktop sequencer
Illumina MiSeq desktop sequencer
PI Supplied Instrument Name: Illumina MiSeq desktop sequencer PI Supplied Instrument Description:Amplicon sequencing: PCRs were performed on a Life Technologies SimpliAmp Thermal Cycler and sequencing was performed using an Illumina MiSeq desktop sequencer. Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.
Lachat QuickChem 8000 Flow Injection Analysis System
Lachat QuickChem 8000 Flow Injection Analysis System
PI Supplied Instrument Name: Lachat QuickChem 8000 Flow Injection Analysis System PI Supplied Instrument Description:Nitrate-nitrite, Phosphate, Silicate: Lachat QuickChem 8000 Flow Injection Analysis System Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
ThermoFisher Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer
ThermoFisher Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer
PI Supplied Instrument Name: ThermoFisher Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer PI Supplied Instrument Description:Dissolved iron and manganese: ThermoFisher Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Sea-Bird SBE-45
Sea-Bird SBE-45
PI Supplied Instrument Name: Sea-Bird SBE-45 PI Supplied Instrument Description:SST and Salinity: Sea-Bird SBE-45 Instrument Name: Sea-Bird SBE 45 MicroTSG Thermosalinograph Instrument Short Name:SBE 45 MicroTSG Instrument Description: A small externally powered, high-accuracy instrument, designed for shipboard determination of sea surface (pumped-water) conductivity and temperature. It is constructed of plastic and titanium to ensure long life with minimum maintenance. It may optionally be interfaced to an external SBE 38 hull temperature sensor.
Sea Bird SBE 45 MicroTSG (Thermosalinograph) Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0190/
Life Technologies SimpliAmp Thermal Cycler
Life Technologies SimpliAmp Thermal Cycler
PI Supplied Instrument Name: Life Technologies SimpliAmp Thermal Cycler PI Supplied Instrument Description:Amplicon sequencing: PCRs were performed on a Life Technologies SimpliAmp Thermal Cycler and sequencing was performed using an Illumina MiSeq desktop sequencer. Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Turner 10AU fluorometer
Turner 10AU fluorometer
PI Supplied Instrument Name: Turner 10AU fluorometer PI Supplied Instrument Description:Fluorescence: Turner 10AU fluorometer (calibrated on ship using discrete chlorophyll analysis) Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: MV1405
MV1405
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
MV1405
Kenneth W. Bruland
University of California-Santa Cruz
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel