http://lod.bco-dmo.org/id/dataset/876937
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-07-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Smallmouth grunt condition/otolith morphology data and SEM images
2022-07-13
publication
2022-07-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-09-02
publication
https://doi.org/10.26008/1912/bco-dmo.876937.1
Robert J. Holmberg
University of Massachusetts Boston
principalInvestigator
Bradford Bourque
Roger Williams University
principalInvestigator
Eugene D. Gallagher
University of Massachusetts Boston
principalInvestigator
Robyn E. Hannigan
University of Massachusetts Boston
principalInvestigator
Andrew L. Rhyne
Roger Williams University
principalInvestigator
Michael F. Tlusty
University of Massachusetts Boston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Holmberg, R. J., Bourque, B., Gallagher, E. D., Hannigan, R. E., Rhyne, A. L., Tlusty, M. F. (2022) Smallmouth grunt condition/otolith morphology data and SEM images. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-07-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.876937.1 [access date]
Smallmouth Grunt Otolith Morphology Dataset Description: Methods and Sampling: <p>Several clutches of <em>Haemulon chrysargyreum</em> eggs were purchased and shipped from a supplier in Florida, USA, and inspected for quality and development. A clutch was selected, eggs hatched, and larvae distributed among the 20 experimental aquaria at a stocking density of 130 individuals per aquarium. Larvae in each aquarium were subjected to one of 4 seawater pH treatments (8.10, 7.80, 7.60, 7.30) randomly assigned and replicated 5x. Seawater temperature in all aquaria was held constant at 28 C. Aquaria were filled with sterilized natural seawater, and 25% water changes were completed every 48 hrs. Larvae were fed ad libitum with wild copepods from monoculture (<em>Pseudodiaptomus spp.</em>) in a background of live microalgae (<em>Isochrysis spp.</em>). Larvae were reared under experimental conditions for 30 days until the majority achieved settlement competency.</p>
<p>Upon completion of the experimental trial, all surviving fish were removed from each aquarium, euthanized with a lethal dose of tricaine mesylate (MS-222) in seawater, and counted. Survival counts were subtracted from the initial stocking density to calculate mortality counts (see Related Dataset).</p>
<p>Smallmouth grunt otoliths were sampled and quantified for morphological variables according to the methodology reported in Holmberg et al. 2019 (earlier project). Briefly, otoliths were extracted from each experimental fish using microsurgical dissection techniques and photographed using a polarizing dissection microscope for morphometric analysis. Then, otoliths were mounted to aluminum stubs, coated with gold, and imaged using a scanning electron microscope for mineralogical analysis. The attached file "<span style="font-size:13px">images.tar.gz" contains the SEM images.</span></p>
<p>The experimental trial took place between November and December 2013. The otolith morphology, fish length, and fish mortality data were collected over a 5-year span between January 2014 and August 2019.</p>
Funding provided by NSF Emerging Frontiers Division (NSF EF) Award Number: EF-1220480 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220480
completed
Robert J. Holmberg
University of Massachusetts Boston
207-259-5086
PO Box 83
Beals
ME
04611
USA
rholmberg@downeastinstitute.org
pointOfContact
Bradford Bourque
Roger Williams University
1 Old Ferry Rd
Bristol
RI
02809
USA
bbourque@rwu.edu
pointOfContact
Eugene D. Gallagher
University of Massachusetts Boston
617-287-7453
100 William T Morrissey Blvd.
Boston
MA
02125
USA
Eugene.Gallagher@umb.edu
pointOfContact
Robyn E. Hannigan
University of Massachusetts Boston
315-268-6544
Box 5505 8 Clarkson Ave
Potsdam
NY
13699
USA
rhanniga@clarkson.edu
pointOfContact
Andrew L. Rhyne
Roger Williams University
401-254-5750
Dept. of Biology 1 Old Ferry Rd.
Bristol
RI
02809
USA
arhyne@rwu.edu
pointOfContact
Michael F. Tlusty
University of Massachusetts Boston
617-287-7444
100 William T Morrissey Blvd.
Boston
MA
02125
USA
michael.tlusty@umb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ID
FISH
TANK
OBS
OTIE
OSIDE
OTYPE
SETPOINT
pCO2
AREA
PERIM
MINAX
MAJAX
CIRC
SL
LATD
VISC
VATR
FING
LOBES
BAD_IMAGE
Olympus Polarizing Dissection Microscope
JEOL JSM-6010LA IntouchScope Scanning Electron Microscope
theme
None, User defined
sample identification
tank
sample description
pH
Partial pressure of CO2
surface_area
length
count
quality flag
featureType
BCO-DMO Standard Parameters
Microscope - Optical
Scanning Electron Microscope
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Effects on Morphology and Mineralogy in Otoliths of Larval Reef Fish
https://osprey.bco-dmo.org/project/2240
Ocean Acidification: Effects on Morphology and Mineralogy in Otoliths of Larval Reef Fish
<p>If a larval fish cannot avoid predators and cannot orient itself in three-dimensional space, the consequences to the individual and the population are dramatic. Otoliths (ear stones), formed precipitation of calcium carbonate from a bicarbonate-rich and alkaline pH fluid, are critical to fish movement and orientation. Although marine fish compensate for carbon dioxide levels in the surrounding waters little is known about how increased dissolved carbon dioxide and changes in bicarbonate concentrations will impact the formation of otoliths. Increasing atmospheric carbon dioxide concentrations, leading to decreased ocean pH (ocean acidification) may have profound impact on the deposition, growth and function of these critical structures, particularly in larval fish. Focusing on pre-settlement age larval reef fish (<em>Amphiprion clarkii</em> and <em>Chrysiptera parasema</em>), this research integrates expertise in carbonate mineralogy, otolith development, and reef fish biology and leverages this unique combination of expertise to answer fundamental questions regarding the impact of ocean acidification on the structure and function of otoliths.</p>
<p><strong>Specifically, the research will answer two fundamental questions: What are the natural morphological and mineralogical variations within growing otoliths? How do these change when larvae are exposed to high dissolved carbon dioxide concentrations?</strong> Larvae will be hatched and reared under high carbon dioxide-induced low pH and three types of otoliths (sagittae, lapilli, asterisci) will be extracted over the duration of the experiments. Changes in calcium carbonate mineralogy from aragonite (most common) to vaterite (less common, less dense) as well as changes in crystal habit (well formed to poorly formed) will be evaluated using a combination of microscopic and morphometric techniques. The gap in understanding of otolith morphology and mineralogy precludes our ability to accurately evaluate the impact of ocean acidification on larval fish survival. Given that we know very little about the morphology and mineralogy of all three otolith types in larval marine fish, this research will provide fundamental data regarding natural variability. Data from unexposed and exposed larvae will inform our understanding of the development of otoliths and structure-function relationships. Additionally, otoliths provide long-term records of environmental life histories that could be better exploited if we understood the relation between environmental conditions and otolith morphology and mineralogy.</p>
<p>This research represents a unique interdisciplinary collaboration between faculty and students at the University of Massachusetts Boston (a minority-serving institution), New England Aquarium (NEAq; not-for-profit research aquarium), and, through a formal partnership with NEAq, Roger Williams University (primarily undergraduate institution).</p>
OA-OTO MIN
largerWorkCitation
project
eng; USA
oceans
2013-11-01
2019-08-01
Laboratory-based
0
BCO-DMO catalogue of parameters from Smallmouth grunt condition/otolith morphology data and SEM images
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/876965.rdf
Name: ID
Units: unitless
Description: <p>Aquaria/Fish/Otolith ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/876966.rdf
Name: FISH
Units: unitless
Description: <p>Aquaria/Fish ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/876967.rdf
Name: TANK
Units: unitless
Description: <p>Aquaria ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/876968.rdf
Name: OBS
Units: unitless
Description: <p>Fish ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/876969.rdf
Name: OTIE
Units: unitless
Description: <p>Otolith Side/Type</p>
http://lod.bco-dmo.org/id/dataset-parameter/876970.rdf
Name: OSIDE
Units: unitless
Description: <p>Otolith Side</p>
http://lod.bco-dmo.org/id/dataset-parameter/876971.rdf
Name: OTYPE
Units: unitless
Description: <p>Otolith Type</p>
http://lod.bco-dmo.org/id/dataset-parameter/876972.rdf
Name: SETPOINT
Units: pH units
Description: <p>Seawater pH Setpoint</p>
http://lod.bco-dmo.org/id/dataset-parameter/876973.rdf
Name: pCO2
Units: microatmospheres (uatm)
Description: <p>Calculated Seawater pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/876974.rdf
Name: AREA
Units: square micrometers (um^2)
Description: <p>Otolith Surface Area</p>
http://lod.bco-dmo.org/id/dataset-parameter/876975.rdf
Name: PERIM
Units: micrometers (um)
Description: <p>Otolith Perimeter</p>
http://lod.bco-dmo.org/id/dataset-parameter/876976.rdf
Name: MINAX
Units: micrometers (um)
Description: <p>Otolith Minor Axis</p>
http://lod.bco-dmo.org/id/dataset-parameter/876977.rdf
Name: MAJAX
Units: micrometers (um)
Description: <p>Otolith Major Axis</p>
http://lod.bco-dmo.org/id/dataset-parameter/876978.rdf
Name: CIRC
Units: unitless
Description: <p>Otolith Circularity</p>
http://lod.bco-dmo.org/id/dataset-parameter/876979.rdf
Name: SL
Units: millimeters (mm)
Description: <p>Fish Standard Length</p>
http://lod.bco-dmo.org/id/dataset-parameter/876980.rdf
Name: LATD
Units: convexity score
Description: <p>Otolith Lateral Development</p>
http://lod.bco-dmo.org/id/dataset-parameter/876981.rdf
Name: VISC
Units: percent (%) crystals
Description: <p>Otolith Visible Crystals</p>
http://lod.bco-dmo.org/id/dataset-parameter/876982.rdf
Name: VATR
Units: percent (%) vaterite
Description: <p>Otolith Vaterite Replacement</p>
http://lod.bco-dmo.org/id/dataset-parameter/876983.rdf
Name: FING
Units: fingers
Description: <p>Otolith Finger Count</p>
http://lod.bco-dmo.org/id/dataset-parameter/876984.rdf
Name: LOBES
Units: lobes
Description: <p>Otolith Lobe Count</p>
http://lod.bco-dmo.org/id/dataset-parameter/876985.rdf
Name: BAD_IMAGE
Units: unitless
Description: <p>Values of GOOD or BAD to indicate if the SEM Micrograph is usable</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
33806778917
https://darchive.mblwhoilibrary.org/bitstream/1912/29314/1/images.tar.gz
download
267355
https://darchive.mblwhoilibrary.org/bitstream/1912/29314/2/dataset-876937_smallmouth-grunt-otolith-morphology__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.876937.1
download
onLine
dataset
<p>Several clutches of <em>Haemulon chrysargyreum</em> eggs were purchased and shipped from a supplier in Florida, USA, and inspected for quality and development. A clutch was selected, eggs hatched, and larvae distributed among the 20 experimental aquaria at a stocking density of 130 individuals per aquarium. Larvae in each aquarium were subjected to one of 4 seawater pH treatments (8.10, 7.80, 7.60, 7.30) randomly assigned and replicated 5x. Seawater temperature in all aquaria was held constant at 28 C. Aquaria were filled with sterilized natural seawater, and 25% water changes were completed every 48 hrs. Larvae were fed ad libitum with wild copepods from monoculture (<em>Pseudodiaptomus spp.</em>) in a background of live microalgae (<em>Isochrysis spp.</em>). Larvae were reared under experimental conditions for 30 days until the majority achieved settlement competency.</p>
<p>Upon completion of the experimental trial, all surviving fish were removed from each aquarium, euthanized with a lethal dose of tricaine mesylate (MS-222) in seawater, and counted. Survival counts were subtracted from the initial stocking density to calculate mortality counts (see Related Dataset).</p>
<p>Smallmouth grunt otoliths were sampled and quantified for morphological variables according to the methodology reported in Holmberg et al. 2019 (earlier project). Briefly, otoliths were extracted from each experimental fish using microsurgical dissection techniques and photographed using a polarizing dissection microscope for morphometric analysis. Then, otoliths were mounted to aluminum stubs, coated with gold, and imaged using a scanning electron microscope for mineralogical analysis. The attached file "<span style="font-size:13px">images.tar.gz" contains the SEM images.</span></p>
<p>The experimental trial took place between November and December 2013. The otolith morphology, fish length, and fish mortality data were collected over a 5-year span between January 2014 and August 2019.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
Otolith morphology data were&nbsp;processed according to Holmberg et al. 2019. Briefly, otolith morphometrics including area, perimeter, and major/minor axes were quantified using ImageJ v1.51n. Circularity was calculated from major/minor axes. Otolith mineralogical variables including lateral development, visible crystals, vaterite replacement, finger counts, and lobe counts were scored from SEM micrographs according to a rubric designed for this project.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- modified column names to comply with BCO-DMO naming conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus Polarizing Dissection Microscope
Olympus Polarizing Dissection Microscope
PI Supplied Instrument Name: Olympus Polarizing Dissection Microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
JEOL JSM-6010LA IntouchScope Scanning Electron Microscope
JEOL JSM-6010LA IntouchScope Scanning Electron Microscope
PI Supplied Instrument Name: JEOL JSM-6010LA IntouchScope Scanning Electron Microscope Instrument Name: Scanning Electron Microscope Instrument Short Name:SEM Instrument Description: A scanning electron microscope (SEM) scans a focused electron beam over a surface to create an image. The electrons in the beam interact with the sample, producing various signals that can be used to obtain information about the surface topography and composition.