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Viral infections of marine microbes can transform the fate of microbial populations that fuel global ocean biogeochemical cycles. For example, viral infections of microbes lead to the release of carbon and nutrients back into the environment. This regeneration of carbon and nutrients stimulates the activity of other microbes and diverts carbon and nutrients from larger organisms in marine food webs. Because virus-microbe infections are relatively specific, it is critical to identify those pairs of viruses and microbes that may disproportionally contribute to the turnover of carbon and nutrients in the ocean. This project will develop quantitative approaches and tools to quantify which viruses infect which microbes and to use these data to quantify how viral infections of microbes collectively shape nutrient and carbon cycles in the North Atlantic Ocean. The project will analyze virus-microbe interactions in mesocosms at the Bigelow Laboratory for Ocean Sciences in mid-coast Maine and during open ocean expeditions to the Bermuda Atlantic Time-Series Study (BATS) site. An interdisciplinary team will leverage recent advances in molecular biology, computational biology, and mathematical modeling to identify virus-host partners and their impact on the movement of elements through marine systems. This project will support three graduate students, six undergraduate students and one postdoctoral researcher in an interdisciplinary context. Research advances will be translated into reproducible software methods to be disseminated via the community cyberinfrastructure platform iVirus, with additional training materials presented as part of a viral methods and informatics workshop held at The Ohio State University. The translation of discoveries to the public will be furthered by the involvement of journalism undergraduate students at the University of Tennessee-Knoxville.&lt;/p&gt;
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http://lod.bco-dmo.org/id/dataset-parameter/877127.rdf
	Name: corrected_depth
	Units: meters (m)
	Description: &lt;p&gt;Height relative to sea surface&lt;/p&gt; 
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	Name: par_value
	Units: microEinsteins per squre centimeter per second (uE/cm^2s)
	Description: &lt;p&gt;PAR&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/877129.rdf
	Name: par_relative
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	Name: temp
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	Units: grams per kilogram (g/kg)
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http://lod.bco-dmo.org/id/dataset-parameter/877132.rdf
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	Units: kilograms per cubic meter (kg/m^3)
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http://lod.bco-dmo.org/id/dataset-parameter/877133.rdf
	Name: fluor
	Units: milligrams per cubic meter (mg/m^3)
	Description: &lt;p&gt;Wet Labs ECO Chlorophyll Fluorescence&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/877134.rdf
	Name: beam
	Units: reciprocal meters (1/m)
	Description: &lt;p&gt;Wet Labs C-Star Beam Attenuation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/877135.rdf
	Name: oxygen
	Units: micromoles per kilogram (umol/kg)
	Description: &lt;p&gt;Oxygen concentration&lt;/p&gt; 
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	Units: micromoles per kilogram (umol/kg)
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http://lod.bco-dmo.org/id/dataset-parameter/877137.rdf
	Name: oxy_sat
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http://lod.bco-dmo.org/id/dataset-parameter/877138.rdf
	Name: mld
	Units: meters (m)
	Description: &lt;p&gt;Mixed layer depth using a change in sigma-theta from 10dbar of 0.125&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/877139.rdf
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                <gco:CharacterString>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Data Processing:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Data were converted from .hex files to .cnv files using SeaSave on default parameters. Then, each file was read using the seabird python library (&amp;lt;a href=&amp;quot;https://github.com/castelao/seabird&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;https://github.com/castelao/seabird&amp;lt;/a&amp;gt;), and cast latitude, longitude, and deployment time were retrieved. For further calculations, pressure, temperature (primary sensor), conductivity (primary sensor), oxygen (primary sensor), beam attenuation, ECO chlorophyll fluorescence, PAR, corrected PAR, and instrument time were retained. Due to a choppy sea state, data from the upper 5 db were trimmed as the measurements were noisy. Then, all scans from the first 4.5 minutes of the cast were removed as these data were associated with the initializing soak at 15m before profiling. Finally, the upcast was removed to retain the downcast.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Using the python implementation of the Gibbs SeaWater oceanographic toolbox from TEOS-10 (&amp;lt;a href=&amp;quot;https://www.teos-10.org/pubs/gsw/html/gsw_front_page.html&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;https://www.teos-10.org/pubs/gsw/html/gsw_front_page.html&amp;lt;/a&amp;gt;), the following thermodynamic calculations were conducted. A corrected depth (height relative to sea surface, meaning a negative number) was calculated from the pressure and latitude. Then, potential salinity was calculated using conductivity, temperature, and pressure. Absolute salinity was calculated from potential salinity, and conservative temperature was calculated from absolute salinity, temperature, and pressure. Density in terms of sigma-theta was calculated using absolute salinity and conservative temperature. Then, oxygen solubility was calculated using the GSW oxygen solubility function with absolute salinity, conservative temperature, pressure, latitude, and longitude. Oxygen saturation was estimated for each scan using the primary oxygen sensor and the derived oxygen solubility. The outputs for the transformed data for each retained scan are contained in the file [first file]. Then, to remove additional instrument noise and standardize profiles across casts, data were smoothed and interpolated to every 0.5 dbar of pressure with a Nadaraya-Watson kernel estimator using a bandwidth of 5 meters. Using the smoothed and binned outputs, the mixed layer depth was calculated using the criterion of an increase in sigma-theta above 0.125 from 10dbar. Then, the DCM depth was calculated by identifying the depth corresponding to the highest chlorophyll fluorescence below the mixed layer depth. The derived mixed layer and dcm depths, as well as the smoothed and 0.5 dbar binned values for the above mentioned variables are included in the file [file 2].&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Processing:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
- replaced &amp;quot;NA&amp;quot; with &amp;quot;nd&amp;quot; to indicate &amp;quot;no data&amp;quot;;&amp;lt;br /&amp;gt;
- converted date/time field to ISO8601 format;&amp;lt;br /&amp;gt;
- removed the &amp;quot;id&amp;quot; field (identical to &amp;quot;cast&amp;quot;).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/867869.rdf" xlink:title="Biospherical PAR sensor" xlink:actuate="onRequest">Licor PAR sensor (Biospherical Instruments) QSP-2300</gmx:Anchor>
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            <gco:CharacterString>Licor PAR sensor (Biospherical Instruments) QSP-2300</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Licor PAR sensor (Biospherical Instruments) QSP-2300 Instrument Name: Biospherical PAR sensor Instrument Short Name:   Instrument Description: An irradiance sensor designed to measure Photosynthetically Active Radiation (PAR), manufactured by Biospherical Instruments Inc.</gco:CharacterString>
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      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/447.rdf" xlink:title="CTD Sea-Bird" xlink:actuate="onRequest">CTD unit (Seabird) SBE 9+</gmx:Anchor>
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            </gmd:MD_Identifier>
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            <gco:CharacterString>CTD unit (Seabird) SBE 9+</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: CTD unit (Seabird) SBE 9+ Instrument Name: CTD Sea-Bird Instrument Short Name:CTD Sea-Bird   Instrument Description: A Conductivity, Temperature, Depth (CTD) sensor package from SeaBird Electronics. This instrument designation is used when specific make and model are not known or when a more specific term is not available in the BCO-DMO vocabulary. Refer to the dataset-specific metadata for more information about the specific CTD used. More information from: http://www.seabird.com/ Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/580.rdf" xlink:title="CTD-fluorometer" xlink:actuate="onRequest">Fluorometer (Wet Labs) ECO-FLRTD</gmx:Anchor>
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            <gco:CharacterString>Fluorometer (Wet Labs) ECO-FLRTD</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Fluorometer (Wet Labs) ECO-FLRTD Instrument Name: CTD-fluorometer Instrument Short Name:CTD-fluorometer   Instrument Description: A CTD-fluorometer is an instrument package designed to measure hydrographic information (pressure, temperature and conductivity) and chlorophyll fluorescence. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/580.rdf" xlink:title="CTD-fluorometer" xlink:actuate="onRequest">Fluorometer (Chelsea) Aquatracka</gmx:Anchor>
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            </gmd:MD_Identifier>
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            <gco:CharacterString>Fluorometer (Chelsea) Aquatracka</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Fluorometer (Chelsea) Aquatracka Instrument Name: CTD-fluorometer Instrument Short Name:CTD-fluorometer   Instrument Description: A CTD-fluorometer is an instrument package designed to measure hydrographic information (pressure, temperature and conductivity) and chlorophyll fluorescence. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/</gco:CharacterString>
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      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/552.rdf" xlink:title="Sea-Bird SBE 43 Dissolved Oxygen Sensor" xlink:actuate="onRequest">Oxygen sensors (Seabird) SBE 43</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Oxygen sensors (Seabird) SBE 43</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Oxygen sensors (Seabird) SBE 43 Instrument Name: Sea-Bird SBE 43 Dissolved Oxygen Sensor Instrument Short Name:SBE-43 DO   Instrument Description: The Sea-Bird SBE 43 dissolved oxygen sensor is a redesign of the Clark polarographic membrane type of dissolved oxygen sensors. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0036/</gco:CharacterString>
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      <gmi:instrument>
        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/474.rdf" xlink:title="Sea-Bird SBE-4 Conductivity Sensor" xlink:actuate="onRequest">Conductivity sensor (Seabird) SBE 4C</gmx:Anchor>
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            </gmd:MD_Identifier>
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            <gco:CharacterString>Conductivity sensor (Seabird) SBE 4C</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Conductivity sensor (Seabird) SBE 4C Instrument Name: Sea-Bird SBE-4 Conductivity Sensor Instrument Short Name:SBE-4 Conductivity   Instrument Description: The Sea-Bird SBE-4 conductivity sensor is a modular, self-contained instrument that measures conductivity from 0 to 7 Siemens/meter.  The sensors (Version 2; S/N 2000 and higher) have electrically isolated power circuits and optically coupled outputs to eliminate any possibility of noise and corrosion caused by ground loops. The sensing element is a cylindrical, flow-through, borosilicate glass cell with three internal platinum electrodes. Because the outer electrodes are connected together, electric fields are confined inside the cell, making the measured resistance (and instrument calibration) independent of calibration bath size or proximity to protective cages or other objects. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0417/</gco:CharacterString>
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      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/491.rdf" xlink:title="Transmissometer" xlink:actuate="onRequest">Transmissometer (Wet Labs) C-Star</gmx:Anchor>
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            <gco:CharacterString>Transmissometer (Wet Labs) C-Star</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Transmissometer (Wet Labs) C-Star Instrument Name: Transmissometer Instrument Short Name:Transmissometer   Instrument Description: A transmissometer measures the beam attenuation coefficient of the lightsource over the instrument's path-length. This instrument designation is used when specific manufacturer, make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/124/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/647.rdf" xlink:title="Water Temperature Sensor" xlink:actuate="onRequest">Temperature profiler (Seabird) SBE 3F (and 3+ secondary unit)</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
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            <gco:CharacterString>Temperature profiler (Seabird) SBE 3F (and 3+ secondary unit)</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Temperature profiler (Seabird) SBE 3F (and 3+ secondary unit) Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor   Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/647.rdf" xlink:title="Water Temperature Sensor" xlink:actuate="onRequest">Reversing Thermometer (Seabird) SBE 35RT</gmx:Anchor>
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            <gco:CharacterString>Reversing Thermometer (Seabird) SBE 35RT</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Reversing Thermometer (Seabird) SBE 35RT Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor   Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
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            <gmi:description>
              <gco:CharacterString>Cruise: AE2207</gco:CharacterString>
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              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/873946.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AE2207</gmx:Anchor>
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              </gmd:MD_Identifier>
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              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
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              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
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    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="https://bios.asu.edu/research/facilities/atlantic-explorer" xlink:actuate="onRequest">R/V Atlantic Explorer</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33H4" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
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      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
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        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54122.rdf"
           xlink:title="33H4" xlink:actuate="onRequest">R/V Atlantic Explorer</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54122.rdf" xlink:title="R/V Atlantic Explorer" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
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              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/873946.rdf" xlink:title="Cruise" xlink:actuate="onRequest">AE2207</gmx:Anchor>
                    </gmd:title>
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                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51049.rdf" xlink:actuate="onRequest">Steven W. Wilhelm</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/226.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">University of Tennessee Knoxville</gmx:Anchor>
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                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
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     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="https://bios.asu.edu/research/facilities/atlantic-explorer" xlink:actuate="onRequest">R/V Atlantic Explorer</gmx:Anchor>
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    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33H4" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
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        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54122.rdf"
           xlink:title="33H4" xlink:actuate="onRequest">R/V Atlantic Explorer</gmx:Anchor>
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      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54122.rdf" xlink:title="R/V Atlantic Explorer" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
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  </gmi:acquisitionInformation>
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