http://lod.bco-dmo.org/id/dataset/877284
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-07-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Larvae collected near Mariana Back-Arc hydrothermal vents in 2010
2022-08-05
publication
2022-08-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-08-05
publication
https://doi.org/10.26008/1912/bco-dmo.877284.1
Stace Beaulieu
Woods Hole Oceanographic Institution
principalInvestigator
Susan Mills
Woods Hole Oceanographic Institution
principalInvestigator
Lauren S. Mullineaux
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Beaulieu, S., Mills, S., Mullineaux, L. (2022) Larvae collected near Mariana Back-Arc hydrothermal vents in 2010. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-08-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.877284.1 [access date]
Larvae collected near Mariana Back-Arc hydrothermal vents in 2010 Dataset Description: Methods and Sampling: <p><strong>Larval collection:</strong><br />
These larvae were collected near three hydrothermal vent sites (Snail, Archaean, or Urashima) on the Mariana Back-Arc Spreading Center in the southern Mariana Trough in September 2010 on R/V Yokosuka cruise YK10-11. See the Sampling Events&nbsp;Supplemental File for specific locations and dates.</p>
<p>To collect larvae, we deployed large-volume plankton pumps (McLane WTS-LV50, East Falmouth, MA, USA) to sample at 3 or 4 m above the bottom. Each pump sampled for up to 24 hrs at 30 liters per minute over a 63 micron mesh, yielding a filtered volume of up to 41.5 cubic meters. We provide as a supplemental data file a table of sampling events matched to Station Information in the Marine Geoscience Data System (<a href="https://www.marine-geo.org/tools/search/Events.php?event_set_uid=1185" target="_blank">https://www.marine-geo.org/tools/search/Events.php?event_set_uid=1185</a>).</p>
<p><strong>Shipboard sample processing:</strong><br />
Upon recovery, pump filters were rinsed with filtered seawater into trays on ice and examined live to select some individuals for experiments reported by Beaulieu et al. (2015, DOI:10.1111/maec.12207). Within an hour the trays were poured over a 63-um sieve and washed using 95% non-denatured ethanol into a 250 mL jar. Individuals used in the experiments were saved separately, either frozen or preserved in ethanol.</p>
<p><strong>Laboratory sorting and morphological identification:</strong><br />
Samples were poured over nested 300-um and 63-um sieves and washed into dishes for sorting under a dissecting scope at magnifications up to 50X. Individuals were identified to morphotypes at lowest taxonomic level (original categories for morphospecies). All morphospecies were enumerated except for ostracods and copepods which were only accounted as “p” present, “a” absent, or “n” not accounted for. We used the original category plus genetic evidence when available to match our lowest-level identification to the World Register of Marine Species (WoRMS) taxonomic database. Some morphotypes contain morphologically indistinguishable larvae from multiple species (e.g., complex nectochaetes); in this case, our final categories for morphospecies group those individuals with genetic evidence into the higher taxon rank. The table is organized such that the first row listing a final category morphospecies is at the higher taxon rank containing the subsequent rows for that category (in general, the subsequent rows are for specimens sent out for genetic analyses). Note that the polychaetes classified to Infraclass Scolecida correspond to the “Capitellid” larval morphotype in Beaulieu et al. (2015). We thank Dr. Takenori Sasaki for guidance for identification of gastropods.</p>
<p><strong>Additional genetic evidence to assist with larval identification:</strong><br />
A subset of these specimens with 28S rRNA sequences is described in a separate BCO-DMO dataset (Beaulieu et al., 2021, DOI:10.26008/1912/bco-dmo.839476.1, <a href="https://www.bco-dmo.org/dataset/839476" target="_blank">https://www.bco-dmo.org/dataset/839476</a>). We'd like to thank Dr. Hiroka Hidaka, Dr. Shigeaki Kojima, and Dr. Hiromi Watanabe for providing histone 3 sequences for gastropod specimens and Dr. Florence Pradillon for providing cytochrome oxidase subunit 1 (CO1) sequences for polychaete specimens. We thank Bethany Fleming for NCBI BLAST query.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1028862 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1028862
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1947735 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1947735
completed
Stace Beaulieu
Woods Hole Oceanographic Institution
508-289-3536
266 Woods Hole Road Mail Stop #34
Woods Hole
MA
02543
USA
sbeaulieu@whoi.edu
pointOfContact
Susan Mills
Woods Hole Oceanographic Institution
266 Woods Hole Road MS 34
Woods Hole
MA
0254
USA
smills@whoi.edu
pointOfContact
Lauren S. Mullineaux
Woods Hole Oceanographic Institution
508-289-2898
Redfield 1-20 MS #34
Woods Hole
MA
02543
USA
lmullineaux@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Fleming_etal_Table
scientificName
LSID
taxonRank
final_category_morphospecies
original_category_morphospecies
individuals_with_genetic_sequences
occurrenceID
Pump1_Snail_300
Pump1_Snail_63
Pump1_Snail_ship
Pump2_Snail_300
Pump2_Snail_63
Pump2_Snail_ship
Pump3_Archaean_300
Pump3_Archaean_63
Pump3_Archaean_ship
Pump4_Archaean_300
Pump4_Archaean_63
Pump4_Archaean_ship
Pump5_Urashima_300
Pump5_Urashima_63
Pump5_Urashima_ship
Pump6_Urashima_300
Pump6_Urashima_63
Pump6_Urashima_ship
Kingdom
Phylum
Class
Order
Family
Genus
Species
McLane WTS-LV50
dissecting scope
theme
None, User defined
No BCO-DMO term
taxon
Life Science Identifier (LSID)
sample identification
count
kingdom
phylum
taxonomic class
order
family
genus
species scientific name (binomial)
featureType
BCO-DMO Standard Parameters
McLane Large Volume Pumping System WTS-LV
Microscope - Optical
instrument
BCO-DMO Standard Instruments
YK10-11
service
Deployment Activity
Southern Mariana Trough
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RAPID: Larval Abundance, Behavior and Dispersal at Deep-sea Hydrothermal Vents in the Southern Mariana Trough
https://osprey.bco-dmo.org/project/767733
RAPID: Larval Abundance, Behavior and Dispersal at Deep-sea Hydrothermal Vents in the Southern Mariana Trough
<p><em>NSF Award Abstract:</em><br />
Summary: Since the discovery of deep-sea hydrothermal vents over thirty years ago, scientists have been perplexed by the question: How are these vent sites colonized and, more specifically, How are the faunal populations established and maintained at these very discrete and often ephemeral habitats. For animals that are sessile or have limited mobility as adults, dispersal to these habitats occurs early in the life cycle, as planktonic larvae in the water column. Due to the difficulties in sampling deep-sea larvae, including low abundances (dilute concentrations), we have very few quantitative estimates of larval dispersal between or larval supply to hydrothermal vents. We also have little to no knowledge of the behavior of vent larvae. The PIs will use large-volume plankton pumps to collect larvae near vents in the southern Mariana Trough in a collaborative effort to quantify larval abundance, behavior, and dispersal in this little-studied region. The collaboration combines the PI's strengths in the collection and morphological identification of larvae and quantifying and modeling dispersal between deep-sea vents, and those of Japanese partners in rearing larvae of hydrothermal vent fauna, molecular genetic identification of larvae, and population genetics of vent fauna.</p>
<p>Intellectual merit: The southern Mariana Trough is a very interesting region in which to study dispersal of vent-endemic fauna, due to the proximity of vents in the back-arc spreading center to vents along the Mariana Arc. These two tectonic settings create different habitat conditions and support vent communities with different species composition. Vent sites the PIs will visit, in the axis and just off-axis of the back-arc spreading center are as close as 25 km to vents on the arc, yet 600 km south of the other known vents in the back-arc. In addition to the new information on larval abundance, diversity, behavior, and dispersal that will be gained for this little-studied region of the world's ridge system, this project has direct relevance to the integration and synthesis goals of the U.S. Ridge 2000 Program. The PI's lab group has conducted previous work at the Ridge 2000 East Pacific Rise (EPR) Integrated Studies Site (ISS). They will be making a direct comparison of the larval abundance and diversity at the EPR ISS to this very different setting along the global 'baseball seam' of oceanic spreading centers. No other such comparison has been possible due to the lack of sampling effort for larvae with large-volume pumps. Also, they are proposing the first experiments with live vent larvae (to the best of our knowledge - with the exception of brachyuran megalopae at 1 atm) to estimate swimming and sinking rates that are important for adding behavioral information to models of larval dispersal.</p>
<p>Broader Impacts: The project involves reciprocal training and cultural exchange - the PIs will learn field and laboratory research techniques from the Japanese PIs, and they will learn from the U.S. PIs. The project will also benefit the career development of a junior researcher (Beaulieu). The proposed activity broadens the participation of both U.S. and Japanese women scientists in sea-going, oceanographic research. The PIs will broadcast the cruise activities in a web log posted by the international InterRidge Program Office, and they anticipate at least three scientific publications will emerge. New species will be added to the online photographic identification guide for vent larvae and included in the second edition of the printed guide.</p>
<p>Additional cruise data and information are available from MGDS: <a href="http://www.marine-geo.org/tools/search/entry.php?id=YK10-11" target="_blank">http://www.marine-geo.org/tools/search/entry.php?id=YK10-11</a></p>
Mariana Back-arc Vents
largerWorkCitation
project
RUI: Collaborative: The Predictive Nature of Microbial Biofilms for Cuing Larval Settlement at Deep-Sea Hydrothermal Vents
https://osprey.bco-dmo.org/project/851182
RUI: Collaborative: The Predictive Nature of Microbial Biofilms for Cuing Larval Settlement at Deep-Sea Hydrothermal Vents
<p><em>NSF Award Abstract:</em><br />
Over four decades of research have shown that tiny free-swimming offspring of the unique inhabitants of hydrothermal vents can disperse effectively between their specialized habitats. Yet, we know almost nothing about how these larval animals complete the journey by locating and settling down in suitable locations. This question remains one of the key unresolved puzzles in the ecology of the deep sea and is becoming increasingly important to solve as hydrothermal vents are becoming threatened by human impacts. The investigators suggest that the films of bacteria that first form at vents are good signposts for settlement of larvae because they indicate that the hydrothermal vents are suitable for life. This project uses a combined program of field experiments, cutting-edge molecular biology techniques, and shipboard experiments with hydrothermal-vent larvae and cultured bacterial films. The project also connects undergraduate research interns at a primarily undergraduate institution (Western Washington University) with undergraduate research interns at two research institutions (Rutgers and Woods Hole Oceanographic Institution) while working on the project at sea together. Finally, the team is producing a science-in-action documentary filled with ocean science and exploration intended for television distribution and museum screenings. The investigators are using footage of the deep-sea vents, shipboard and diving operations, and laboratory work to create a documentary that highlights the foundation of scientific research—hypothesis-driven research, the application of the scientific method, and the importance of critical thinking—all in the framework of the study of an exciting, but threatened, ecosystem.</p>
<p>Hydrothermal vents are particularly tractable systems in which to study questions about the roles of biofilms in larval settlement because biofilms at vents are relatively low-complexity; vent animals are strictly dependent on vent microbes, often through symbiotic partnerships acquired after settlement; and environmental variations are present within the range of a common larval pool. Moreover, decades of research on settlement in model organisms give us good insight into biofilm cues; there is solid foundational understanding about colonization patterns at vents; we now have excellent tools to collect, identify, and culture vent larvae and microbes; and modern environmental "-omics" techniques are a good tool to characterize biological cues produced by biofilms. The project provides an unprecedented, quantitative look into the role of microbial biofilms in structuring larval settlement at hydrothermal vents, achieved only through the close collaboration of microbial and larval ecologists. The combined field program of short-term settlement experiments, microbial "-omics" work, and subsequent shipboard settlement experiments allows the investigative team to use field experiments to statistically model the factors that best predict larval settlement in the field, then test those predictions with shipboard experiments that decouple covarying conditions. This extensive characterization of putative larval settlement cues and their relationship to colonization success in heterogeneous vent habitat niches will contribute to a broader understanding of colonization success across diverse marine ecosystems. Understanding the role that the initial settlement of larvae plays in the recovery and resilience of hydrothermal-vent ecosystems is critical to developing informed management plans for deep-sea mining.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Vent Settlement Cues
largerWorkCitation
project
eng; USA
oceans
Southern Mariana Trough
143.6188
143.6485
12.9225
12.9526
2010-09-05
2010-09-11
From projects that focused on the following 2 locations: 1. Southern Mariana Trough 2. East Pacific Rise, 9 North hydrothermal vents
0
BCO-DMO catalogue of parameters from Larvae collected near Mariana Back-Arc hydrothermal vents in 2010
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/877784.rdf
Name: Fleming_etal_Table
Units: unitless
Description: <p>Category with some morphospecies grouped for analysis by Fleming et al. manuscript in prep.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877785.rdf
Name: scientificName
Units: unitless
Description: <p>Scientific name at the lowest level that matches original_category_morphospecies from the World Register of Marine Species that pairs to the LSID Darwin Core term <a href="http://rs.tdwg.org/dwc/terms/scientificName">http://rs.tdwg.org/dwc/terms/scientificName</a></p>
http://lod.bco-dmo.org/id/dataset-parameter/877786.rdf
Name: LSID
Units: unitless
Description: <p>Life Science Identifier for the AphiaID in the World Register of Marine Species that pairs to the scientificName</p>
http://lod.bco-dmo.org/id/dataset-parameter/877787.rdf
Name: taxonRank
Units: unitless
Description: <p>Taxon rank for the scientificName in the World Register of Marine Species Darwin Core term <a href="http://rs.tdwg.org/dwc/terms/taxonRank">http://rs.tdwg.org/dwc/terms/taxonRank</a></p>
http://lod.bco-dmo.org/id/dataset-parameter/877788.rdf
Name: final_category_morphospecies
Units: unitless
Description: <p>Final category for determining abundance from morphological sorting. Some individuals in this morphospecies may be identified to lower taxonomic level but that could not be applied to all individuals.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877789.rdf
Name: original_category_morphospecies
Units: unitless
Description: <p>Original category for morphological sorting to lowest taxonomic level</p>
http://lod.bco-dmo.org/id/dataset-parameter/877790.rdf
Name: individuals_with_genetic_sequences
Units: unitless
Description: <p>Identifiers for individuals with genetic sequence data. Those listed with occurrenceID match to TubeID in DOI:10.26008/1912/bco-dmo.839476.1, otherwise gastropod identifiers for H. Hidaka, polychaete identifiers for F. Pradillon.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877791.rdf
Name: occurrenceID
Units: unitless
Description: <p>A unique identifier for the Occurrence record in DOI:10.26008/1912/bco-dmo.839476.1 provided to OBIS and GBIF that concatenates the eventID with the individual identifier Darwin Core term <a href="http://rs.tdwg.org/dwc/terms/occurrenceID">http://rs.tdwg.org/dwc/terms/occurrenceID</a></p>
http://lod.bco-dmo.org/id/dataset-parameter/877792.rdf
Name: Pump1_Snail_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 1 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877793.rdf
Name: Pump1_Snail_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 1 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877794.rdf
Name: Pump1_Snail_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 1 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877795.rdf
Name: Pump2_Snail_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 2 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877796.rdf
Name: Pump2_Snail_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 2 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877797.rdf
Name: Pump2_Snail_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 2 at locality Snail</p>
http://lod.bco-dmo.org/id/dataset-parameter/877798.rdf
Name: Pump3_Archaean_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 3 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877799.rdf
Name: Pump3_Archaean_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 3 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877800.rdf
Name: Pump3_Archaean_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 3 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877801.rdf
Name: Pump4_Archaean_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 4 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877802.rdf
Name: Pump4_Archaean_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 4 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877803.rdf
Name: Pump4_Archaean_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 4 at locality Archaean</p>
http://lod.bco-dmo.org/id/dataset-parameter/877804.rdf
Name: Pump5_Urashima_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 5 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877805.rdf
Name: Pump5_Urashima_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 5 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877806.rdf
Name: Pump5_Urashima_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 5 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877807.rdf
Name: Pump6_Urashima_300
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals retained on 300 um sieve from pump mooring deployment 6 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877808.rdf
Name: Pump6_Urashima_63
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals that passed through 300 um retained on 63 um sieve from pump mooring deployment 6 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877809.rdf
Name: Pump6_Urashima_ship
Units: Integer count or p,a,n ("present", "absent", "not accounted for")
Description: <p>Count for individuals sorted live onboard ship from pump mooring deployment 6 at locality Urashima</p>
http://lod.bco-dmo.org/id/dataset-parameter/877810.rdf
Name: Kingdom
Units: unitless
Description: <p>Kingdom for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877811.rdf
Name: Phylum
Units: unitless
Description: <p>Phylum for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877812.rdf
Name: Class
Units: unitless
Description: <p>Class for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877813.rdf
Name: Order
Units: unitless
Description: <p>Order for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877814.rdf
Name: Family
Units: unitless
Description: <p>Family for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877815.rdf
Name: Genus
Units: unitless
Description: <p>Genus for the scientificName in the World Register of Marine Species</p>
http://lod.bco-dmo.org/id/dataset-parameter/877816.rdf
Name: Species
Units: unitless
Description: <p>Species for the scientificName in the World Register of Marine Species</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
19070
https://darchive.mblwhoilibrary.org/bitstream/1912/29197/1/dataset-877284_larvae-collected-near-mariana-back-arc-hydrothermal-vents-2010__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.877284.1
download
onLine
dataset
<p><strong>Larval collection:</strong><br />
These larvae were collected near three hydrothermal vent sites (Snail, Archaean, or Urashima) on the Mariana Back-Arc Spreading Center in the southern Mariana Trough in September 2010 on R/V Yokosuka cruise YK10-11. See the Sampling Events&nbsp;Supplemental File for specific locations and dates.</p>
<p>To collect larvae, we deployed large-volume plankton pumps (McLane WTS-LV50, East Falmouth, MA, USA) to sample at 3 or 4 m above the bottom. Each pump sampled for up to 24 hrs at 30 liters per minute over a 63 micron mesh, yielding a filtered volume of up to 41.5 cubic meters. We provide as a supplemental data file a table of sampling events matched to Station Information in the Marine Geoscience Data System (<a href="https://www.marine-geo.org/tools/search/Events.php?event_set_uid=1185" target="_blank">https://www.marine-geo.org/tools/search/Events.php?event_set_uid=1185</a>).</p>
<p><strong>Shipboard sample processing:</strong><br />
Upon recovery, pump filters were rinsed with filtered seawater into trays on ice and examined live to select some individuals for experiments reported by Beaulieu et al. (2015, DOI:10.1111/maec.12207). Within an hour the trays were poured over a 63-um sieve and washed using 95% non-denatured ethanol into a 250 mL jar. Individuals used in the experiments were saved separately, either frozen or preserved in ethanol.</p>
<p><strong>Laboratory sorting and morphological identification:</strong><br />
Samples were poured over nested 300-um and 63-um sieves and washed into dishes for sorting under a dissecting scope at magnifications up to 50X. Individuals were identified to morphotypes at lowest taxonomic level (original categories for morphospecies). All morphospecies were enumerated except for ostracods and copepods which were only accounted as “p” present, “a” absent, or “n” not accounted for. We used the original category plus genetic evidence when available to match our lowest-level identification to the World Register of Marine Species (WoRMS) taxonomic database. Some morphotypes contain morphologically indistinguishable larvae from multiple species (e.g., complex nectochaetes); in this case, our final categories for morphospecies group those individuals with genetic evidence into the higher taxon rank. The table is organized such that the first row listing a final category morphospecies is at the higher taxon rank containing the subsequent rows for that category (in general, the subsequent rows are for specimens sent out for genetic analyses). Note that the polychaetes classified to Infraclass Scolecida correspond to the “Capitellid” larval morphotype in Beaulieu et al. (2015). We thank Dr. Takenori Sasaki for guidance for identification of gastropods.</p>
<p><strong>Additional genetic evidence to assist with larval identification:</strong><br />
A subset of these specimens with 28S rRNA sequences is described in a separate BCO-DMO dataset (Beaulieu et al., 2021, DOI:10.26008/1912/bco-dmo.839476.1, <a href="https://www.bco-dmo.org/dataset/839476" target="_blank">https://www.bco-dmo.org/dataset/839476</a>). We'd like to thank Dr. Hiroka Hidaka, Dr. Shigeaki Kojima, and Dr. Hiromi Watanabe for providing histone 3 sequences for gastropod specimens and Dr. Florence Pradillon for providing cytochrome oxidase subunit 1 (CO1) sequences for polychaete specimens. We thank Bethany Fleming for NCBI BLAST query.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
Counts per morphospecies were entered into an electronic online spreadsheet (Google sheet) that was downloaded to a local .xlsx copy. The scientificName column was used with the WoRMS Taxon Match Tool to obtain a text file with the LSID and taxonomic classification and to confirm taxon status accepted on 2022-02-12. The larval_counts sheet in the .xlsx and the Taxon Match .txt were read into an R programming script to join columns into the larval counts data table <a href="https://github.com/sbeaulieu/EPR-traits/blob/master/Mariana_larval_counts_BCODMO.R" target="_blank">https://github.com/sbeaulieu/EPR-traits/blob/master/Mariana_larval_counts_BCODMO.R</a>. Another sheet in the .xlsx was exported to a csv file as the sampling event table. The data tables are partially aligned to Darwin Core so that they ultimately can be provided to the Ocean Biodiversity Information System (OBIS) and Global Biodiversity Information Facility (GBIF). Note that 25 individuals with occurrenceID have already been contributed to OBIS and GBIF.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- replaced "NA" with "nd" (no data);<br />
- renamed fields to comply with BCO-DMO naming conventions;<br />
- removed commas from values in columns Fleming_etal_Table, final_category_morphospecies, and original_category_morphospecies;<br />
- changed long text string in original_category_morphospecies, row 13 as requested by PI.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
McLane WTS-LV50
McLane WTS-LV50
PI Supplied Instrument Name: McLane WTS-LV50 Instrument Name: McLane Large Volume Pumping System WTS-LV Instrument Short Name:WTS-LV Instrument Description: The WTS-LV is a Water Transfer System (WTS) Large Volume (LV) pumping instrument designed and manufactured by McLane Research Labs (Falmouth, MA, USA). It is a large-volume, single-event sampler that collects suspended and dissolved particulate samples in situ.
Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.
The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.
dissecting scope
dissecting scope
PI Supplied Instrument Name: dissecting scope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Cruise: YK10-11
YK10-11
R/V Yokosuka
Community Standard Description
International Council for the Exploration of the Sea
R/V Yokosuka
vessel
YK10-11
http://www.godac.jamstec.go.jp/catalog/data/doc_catalog/media/YK10-11_all.pdf
Report describing YK10-11
R/V Yokosuka
Community Standard Description
International Council for the Exploration of the Sea
R/V Yokosuka
vessel