http://lod.bco-dmo.org/id/dataset/877466
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-07-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Total dissolved metal concentrations measured during the 2017-2018 CICLOPS expedition
2022-07-26
publication
2022-07-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-10-05
publication
https://doi.org/10.26008/1912/bco-dmo.877466.1
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kellogg, R., Saito, M. A. (2022) Total dissolved metal concentrations measured during the 2017-2018 CICLOPS expedition. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-07-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.877466.1 [access date]
Dissolved metal concentrations measured during the CICLOPS Dataset Description: Methods and Sampling: <p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong><br />
&nbsp;</p>
<p><em>Study area and sample collection</em></p>
<p>Sample collection occurred during the CICLOPS (Cobalamin and Iron Co-Limitation of Phytoplankton Species) expedition (expedition code NBP18-01; chief scientist G.R. DiTullio) aboard the RVIB Nathaniel B. Palmer, December 11, 2017 – March 3, 2018 in the Amundsen Sea and Ross Sea of the Southern Ocean. Water samples were collected using trace metal sampling protocols described previously (Noble et al. 2012). A trace metal clean rosette suspended on a Kevlar line and equipped with twelve 8L X-Niskin bottles (Ocean Test Equipment) was used to collect seawater at depths ranging from 10 – 600 m.</p>
<p>&nbsp;</p>
<p><em>Preparation of plasticware</em></p>
<p>Polyethylene and polycarbonate sampling and incubation bottles were rigorously cleaned to remove trace metal contaminants before use. Bottles with rinsed with Milli-Q water (Millipore), soaked for 72h in &lt;1% Citranox detergent, rotated, soaked for an additional 72h, and then rinsed five times with Milli-Q water. Bottles were then filled with 10% HCl (Baker instra-analyzed) by volume and soaked for a minimum of one week, rotated, and soaked for another week. Bottles were then rinsed five times with dilute acid (HCl, pH 2) and stored double-bagged in plastic zip bags. All cleaning work was conducted in a Class 100 clean room.</p>
<p>&nbsp;</p>
<p><em>Analyses of total dissolved Cd and Zn using isotope dilution</em></p>
<p>Samples for the analysis of total dissolved Zn, Cd, Fe, Mn, Cu and Ni concentrations were collected shipboard by pressure-filtering X-Niskin bottles through an acid-washed 142mm, 0.2µM Supor membrane filter (Pall) within 3 hours of rosette recovery using high purity (99.999%) N<sub>2</sub> gas. Total dissolved water samples were collected into 250mL trace metal clean polyethylene bottles and were stored double-bagged in plastic zip bags. Seawater samples for <sup>110</sup>Cd and <sup>67</sup>Zn stable isotope uptake experiments were collected in the same way but without filtering. All sample collection occurred shipboard within a trace metal clean van containing laminar flow hoods and plastic sheeting. Samples for total dissolved metal analysis were acidified to pH 1.7 with high purity HCl (Optima, Fisher Scientific) within 7 months of sampling and were stored acidified at room temperature for over 1 year prior to analysis.</p>
<p>Quantification of dissolved metals in samples and reference seawater was performed for total dissolved Fe, Ni, Cu, Zn, and Cd using isotope dilution. 15mL of acidified seawater sample was spiked with 50μL of a stable isotope spike solution artificially enriched in <sup>57</sup>Fe, <sup>61</sup>Ni, <sup>65</sup>Cu, <sup>67</sup>Zn, and <sup>110</sup>Cd. All stable isotopes were received in solid form (Oak Ridge National Laboratory). Initial dissolution and all subsequent dilutions were made using concentrated nitric acid (Optima, Fisher Scientific). Concentrations and spike ratios were verified by ICP-MS using a multi-element standard curve (SPEX CertiPrep). The composition of the isotope spike was made such that the 15mL spiked sample contained the following ratios: <sup>57</sup>Fe/<sup>56</sup>Fe = 0.7, <sup>61</sup>Ni/<sup>60</sup>Ni = 0.5, <sup>65</sup>Cu/<sup>63</sup>Cu = 1, <sup>67</sup>Zn/<sup>66</sup>Zn = 0.7, and <sup>110</sup>Cd/<sup>114</sup>Cd = 1 and were verified with ICP-MS. These ratios were chosen to minimize the uncertainty introduced by error propagation through the isotope dilution equation (Wu and Boyle 1998; Rudge et al. 2009; Tan et al. 2020). Because it is monoisotopic, total dissolved Mn was calculated using a modified isotope dilution equation. This equation and its details can be found under the Supplemental Files section of this metadata landing page within the file titled,&nbsp;<em>Modified Isotope Dilution Equation.</em></p>
<p>In this equation,&nbsp;<sup>55</sup>Mn<sub>spl</sub> and <sup>57</sup>Fe<sub>spl</sub> refer to the blank corrected counts per second (cps) of <sup>55</sup>Mn and <sup>57</sup>Fe in the spiked sample, <sup>57</sup>Fe spike is the concentration of <sup>57</sup>Fe spike, <sup>57</sup>Fe<sub>slope</sub> is the slope of the external standard calibration curve (SPEX curve) relating <sup>57</sup>Fe cps to ppb, and <sup>55</sup>Mn<sub>slope</sub> is the slope of the external calibration curve (SPEX curve) relating <sup>55</sup>Mn cps to ppb. Due to the acidification of seawater prior to ICP-MS analysis, Mn ICP-MS measurements do not include contributions from humic-type Mn(III)-ligand complexes (Oldham et al. 2021). Until the inclusion of Mn(III) is resolved and intercalibrated, we report these Mn values as Mn(II) and note that they are consistent with prior studies employing the same acidification technique (Sedwick et al. 2000; Noble et al. 2013; Gerringa et al. 2020).</p>
<p>Preconcentration of spiked seawater samples for total dissolved metal analysis was performed using the automated solid phase extraction system seaFAST-pico (Elemental Scientific) in offline concentration mode with an initial volume of 15mL and elution volume of 500µL (Bown et al. 2017; Rapp et al. 2017; Jackson et al. 2018; Wuttig et al. 2019). The seaFAST contains a Nobias-chelate PA1 resin column (ethylenediaminetriacete and iminodiacetate) suitable for the simultaneous preconcentration of several trace metals (Fe, Mn, Zn, Cu, Co, Cd, Ni) with high sensitivity and quantitative recovery (Sohrin et al. 2008; Biller and Bruland 2012). Adjusted seaFAST software settings were a 17 second load loop time and a single 10mL load cycle. Process blanks consisted of pH 2 HCl (Optima, Fisher Scientific) and were processed as samples were to account for any contamination introduced by instrument processing.</p>
<p>Reagents consisted of a 1.5M ammonium acetate pH 6.0 buffer made using glacial acetic acid and ammonium hydroxide (20-22%) of the highest purity (Optima, Fisher Chemical), a 1% nitric acid rinse solution (Optima grade, Fisher Chemical), and a 10% nitric acid elution buffer (Optima grade, Fisher Chemical) with 10 ppb indium (<sup>115</sup>In, SPEX CertiPrep) added as an internal standard. Solutions were prepared with 18.2 Ω Milli-Q water (Millipore). Polypropylene 15mL centrifuge tubes used in sample processing were made trace metal clean by soaking in 10% HCl for 5 days and rinsing with pH 2 HCl prior to use.</p>
<p>Following offline seaFAST preconcentration, multielemental quantitative analysis was performed using an iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific). Oxide interference on metal isotopes was minimized through the use of a cooled spray chamber and helium collision gas. Analytes were measured in single quadruple mode (kinetic energy discrimination [KED]). Concentrations of Mn, Fe, Ni, Cu, Zn and Cd were determined using a six-point external standard curve of a multi-element standard (SPEX CertiPrep), diluted to range from 1-10 ppb in 5% nitric acid. An indium standard (SPEX CertiPrep) was similarly added to these standard stocks, diluted to range 1-10 ppb. Instrument injection blanks consisted of 5% nitric acid in Milli-Q. Standard curve R<sup>2</sup> values were ≥0.98 for all metals monitored. Method accuracy and precision were assessed using the 2009 GEOTRACES coastal surface seawater (GSC) standard which produced values consistent with consensus results.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1644073 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1644073
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643684 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643684
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643845 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643845
completed
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Lat_deg_N
Lon_deg_E
Collection_datetime
Bottle
Depth_m
Fe_D_CONC_BOTTLE
Fe_D_CONC_BOTTLE_FLAG
Mn_D_CONC_BOTTLE
Mn_D_CONC_BOTTLE_FLAG
Ni_D_CONC_BOTTLE
Ni_D_CONC_BOTTLE_FLAG
Cu_D_CONC_BOTTLE
Cu_D_CONC_BOTTLE_FLAG
Zn_D_CONC_BOTTLE
Zn_D_CONC_BOTTLE_FLAG
Cd_D_CONC_BOTTLE
Cd_D_CONC_BOTTLE_FLAG
iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific)
Twelve 8L X-Niskin bottles (Ocean Test Equipment)
Automated solid phase extraction system seaFAST-pico
theme
None, User defined
station
latitude
longitude
date
bottle
depth
trace metal concentration
quality flag
featureType
BCO-DMO Standard Parameters
Inductively Coupled Plasma Mass Spectrometer
Niskin bottle
SeaFAST Automated Preconcentration System
instrument
BCO-DMO Standard Instruments
NBP1801
service
Deployment Activity
Western Ross Sea, Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
https://osprey.bco-dmo.org/project/774945
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
<p>NSF abstract:<br />
Phytoplankton blooms in the coastal waters of the Ross Sea, Antarctica are typically dominated by either diatoms or Phaeocystis Antarctica (a flagellated algae that often can form large colonies in a gelatinous matrix). The project seeks to determine if an association of bacterial populations with Phaeocystis antarctica colonies can directly supply Phaeocystis with Vitamin B12, which can be an important co-limiting micronutrient in the Ross Sea. The supply of an essential vitamin coupled with the ability to grow at lower iron concentrations may put Phaeocystis at a competitive advantage over diatoms. Because Phaeocystis cells can fix more carbon than diatoms and Phaeocystis are not grazed as efficiently as diatoms, the project will help in refining understanding of carbon dynamics in the region as well as the basis of the food web webs. Such understanding also has the potential to help refine predictive ecological models for the region. The project will conduct public outreach activities and will contribute to undergraduate and graduate research. Engagement of underrepresented students will occur during summer student internships. A collaboration with Italian Antarctic researchers, who have been studying the Terra Nova Bay ecosystem since the 1980s, aims to enhance the project and promote international scientific collaborations.</p>
<p>The study will test whether a mutualistic symbioses between attached bacteria and Phaeocystis provides colonial cells a mechanism for alleviating chronic Vitamin B12 co-limitation effects thereby conferring them with a competitive advantage over diatom communities. The use of drifters in a time series study will provide the opportunity to track in both space and time a developing algal bloom in Terra Nova Bay and to determine community structure and the physiological nutrient status of microbial populations. A combination of flow cytometry, proteomics, metatranscriptomics, radioisotopic and stable isotopic labeling experiments will determine carbon and nutrient uptake rates and the role of bacteria in mitigating potential vitamin B12 and iron limitation. Membrane inlet and proton transfer reaction mass spectrometry will also be used to estimate net community production and release of volatile organic carbon compounds that are climatically active. Understanding how environmental parameters can influence microbial community dynamics in Antarctic coastal waters will advance an understanding of how changes in ocean stratification and chemistry could impact the biogeochemistry and food web dynamics of Southern Ocean ecosystems.</p>
CICLOPS
largerWorkCitation
project
eng; USA
oceans
Western Ross Sea, Southern Ocean
160
-115
-80
-70
2017-12-30
2018-02-18
Amundsen Sea, Ross Sea, Terra Nova Bay
0
BCO-DMO catalogue of parameters from Total dissolved metal concentrations measured during the 2017-2018 CICLOPS expedition
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/877514.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/877515.rdf
Name: Lat_deg_N
Units: decimal degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/877516.rdf
Name: Lon_deg_E
Units: decimal degrees East
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/877517.rdf
Name: Collection_datetime
Units: unitless; %Y-%m-%d %H:%M
Description: <p>Datetime of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/877518.rdf
Name: Bottle
Units: unitless
Description: <p>Bottle ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/877519.rdf
Name: Depth_m
Units: meters
Description: <p>Depth of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/877520.rdf
Name: Fe_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved iron concentration. This parameter follows GEOTRACES vocabulary for concentrations of dissolved metal</p>
http://lod.bco-dmo.org/id/dataset-parameter/877521.rdf
Name: Fe_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877522.rdf
Name: Mn_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved manganese concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/877523.rdf
Name: Mn_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877524.rdf
Name: Ni_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved nickel concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/877525.rdf
Name: Ni_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877526.rdf
Name: Cu_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved copper concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/877527.rdf
Name: Cu_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877528.rdf
Name: Zn_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved zinc concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/877529.rdf
Name: Zn_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
http://lod.bco-dmo.org/id/dataset-parameter/877530.rdf
Name: Cd_D_CONC_BOTTLE
Units: nanomoles per liter (nM)
Description: <p>Dissolved cadmium concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/877531.rdf
Name: Cd_D_CONC_BOTTLE_FLAG
Units: unitless
Description: <p>Quality flag for dissolved iron concentration. 1= good value, 2= probably good value, 3= probably bad value, 4=bad value, 6=value below detection and changed to 0. Flags are based on GEOTRACES quality flag policy <a href="https://www.geotraces.org/geotraces-quality-flag-policy/">https://www.geotraces.org/geotraces-quality-flag-policy/</a>.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
27147
https://darchive.mblwhoilibrary.org/bitstream/1912/29388/1/dataset-877466_ciclops-dissolved-metals__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.877466.1
download
onLine
dataset
<p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong><br />
&nbsp;</p>
<p><em>Study area and sample collection</em></p>
<p>Sample collection occurred during the CICLOPS (Cobalamin and Iron Co-Limitation of Phytoplankton Species) expedition (expedition code NBP18-01; chief scientist G.R. DiTullio) aboard the RVIB Nathaniel B. Palmer, December 11, 2017 – March 3, 2018 in the Amundsen Sea and Ross Sea of the Southern Ocean. Water samples were collected using trace metal sampling protocols described previously (Noble et al. 2012). A trace metal clean rosette suspended on a Kevlar line and equipped with twelve 8L X-Niskin bottles (Ocean Test Equipment) was used to collect seawater at depths ranging from 10 – 600 m.</p>
<p>&nbsp;</p>
<p><em>Preparation of plasticware</em></p>
<p>Polyethylene and polycarbonate sampling and incubation bottles were rigorously cleaned to remove trace metal contaminants before use. Bottles with rinsed with Milli-Q water (Millipore), soaked for 72h in &lt;1% Citranox detergent, rotated, soaked for an additional 72h, and then rinsed five times with Milli-Q water. Bottles were then filled with 10% HCl (Baker instra-analyzed) by volume and soaked for a minimum of one week, rotated, and soaked for another week. Bottles were then rinsed five times with dilute acid (HCl, pH 2) and stored double-bagged in plastic zip bags. All cleaning work was conducted in a Class 100 clean room.</p>
<p>&nbsp;</p>
<p><em>Analyses of total dissolved Cd and Zn using isotope dilution</em></p>
<p>Samples for the analysis of total dissolved Zn, Cd, Fe, Mn, Cu and Ni concentrations were collected shipboard by pressure-filtering X-Niskin bottles through an acid-washed 142mm, 0.2µM Supor membrane filter (Pall) within 3 hours of rosette recovery using high purity (99.999%) N<sub>2</sub> gas. Total dissolved water samples were collected into 250mL trace metal clean polyethylene bottles and were stored double-bagged in plastic zip bags. Seawater samples for <sup>110</sup>Cd and <sup>67</sup>Zn stable isotope uptake experiments were collected in the same way but without filtering. All sample collection occurred shipboard within a trace metal clean van containing laminar flow hoods and plastic sheeting. Samples for total dissolved metal analysis were acidified to pH 1.7 with high purity HCl (Optima, Fisher Scientific) within 7 months of sampling and were stored acidified at room temperature for over 1 year prior to analysis.</p>
<p>Quantification of dissolved metals in samples and reference seawater was performed for total dissolved Fe, Ni, Cu, Zn, and Cd using isotope dilution. 15mL of acidified seawater sample was spiked with 50μL of a stable isotope spike solution artificially enriched in <sup>57</sup>Fe, <sup>61</sup>Ni, <sup>65</sup>Cu, <sup>67</sup>Zn, and <sup>110</sup>Cd. All stable isotopes were received in solid form (Oak Ridge National Laboratory). Initial dissolution and all subsequent dilutions were made using concentrated nitric acid (Optima, Fisher Scientific). Concentrations and spike ratios were verified by ICP-MS using a multi-element standard curve (SPEX CertiPrep). The composition of the isotope spike was made such that the 15mL spiked sample contained the following ratios: <sup>57</sup>Fe/<sup>56</sup>Fe = 0.7, <sup>61</sup>Ni/<sup>60</sup>Ni = 0.5, <sup>65</sup>Cu/<sup>63</sup>Cu = 1, <sup>67</sup>Zn/<sup>66</sup>Zn = 0.7, and <sup>110</sup>Cd/<sup>114</sup>Cd = 1 and were verified with ICP-MS. These ratios were chosen to minimize the uncertainty introduced by error propagation through the isotope dilution equation (Wu and Boyle 1998; Rudge et al. 2009; Tan et al. 2020). Because it is monoisotopic, total dissolved Mn was calculated using a modified isotope dilution equation. This equation and its details can be found under the Supplemental Files section of this metadata landing page within the file titled,&nbsp;<em>Modified Isotope Dilution Equation.</em></p>
<p>In this equation,&nbsp;<sup>55</sup>Mn<sub>spl</sub> and <sup>57</sup>Fe<sub>spl</sub> refer to the blank corrected counts per second (cps) of <sup>55</sup>Mn and <sup>57</sup>Fe in the spiked sample, <sup>57</sup>Fe spike is the concentration of <sup>57</sup>Fe spike, <sup>57</sup>Fe<sub>slope</sub> is the slope of the external standard calibration curve (SPEX curve) relating <sup>57</sup>Fe cps to ppb, and <sup>55</sup>Mn<sub>slope</sub> is the slope of the external calibration curve (SPEX curve) relating <sup>55</sup>Mn cps to ppb. Due to the acidification of seawater prior to ICP-MS analysis, Mn ICP-MS measurements do not include contributions from humic-type Mn(III)-ligand complexes (Oldham et al. 2021). Until the inclusion of Mn(III) is resolved and intercalibrated, we report these Mn values as Mn(II) and note that they are consistent with prior studies employing the same acidification technique (Sedwick et al. 2000; Noble et al. 2013; Gerringa et al. 2020).</p>
<p>Preconcentration of spiked seawater samples for total dissolved metal analysis was performed using the automated solid phase extraction system seaFAST-pico (Elemental Scientific) in offline concentration mode with an initial volume of 15mL and elution volume of 500µL (Bown et al. 2017; Rapp et al. 2017; Jackson et al. 2018; Wuttig et al. 2019). The seaFAST contains a Nobias-chelate PA1 resin column (ethylenediaminetriacete and iminodiacetate) suitable for the simultaneous preconcentration of several trace metals (Fe, Mn, Zn, Cu, Co, Cd, Ni) with high sensitivity and quantitative recovery (Sohrin et al. 2008; Biller and Bruland 2012). Adjusted seaFAST software settings were a 17 second load loop time and a single 10mL load cycle. Process blanks consisted of pH 2 HCl (Optima, Fisher Scientific) and were processed as samples were to account for any contamination introduced by instrument processing.</p>
<p>Reagents consisted of a 1.5M ammonium acetate pH 6.0 buffer made using glacial acetic acid and ammonium hydroxide (20-22%) of the highest purity (Optima, Fisher Chemical), a 1% nitric acid rinse solution (Optima grade, Fisher Chemical), and a 10% nitric acid elution buffer (Optima grade, Fisher Chemical) with 10 ppb indium (<sup>115</sup>In, SPEX CertiPrep) added as an internal standard. Solutions were prepared with 18.2 Ω Milli-Q water (Millipore). Polypropylene 15mL centrifuge tubes used in sample processing were made trace metal clean by soaking in 10% HCl for 5 days and rinsing with pH 2 HCl prior to use.</p>
<p>Following offline seaFAST preconcentration, multielemental quantitative analysis was performed using an iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific). Oxide interference on metal isotopes was minimized through the use of a cooled spray chamber and helium collision gas. Analytes were measured in single quadruple mode (kinetic energy discrimination [KED]). Concentrations of Mn, Fe, Ni, Cu, Zn and Cd were determined using a six-point external standard curve of a multi-element standard (SPEX CertiPrep), diluted to range from 1-10 ppb in 5% nitric acid. An indium standard (SPEX CertiPrep) was similarly added to these standard stocks, diluted to range 1-10 ppb. Instrument injection blanks consisted of 5% nitric acid in Milli-Q. Standard curve R<sup>2</sup> values were ≥0.98 for all metals monitored. Method accuracy and precision were assessed using the 2009 GEOTRACES coastal surface seawater (GSC) standard which produced values consistent with consensus results.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing Notes from Researcher:&nbsp;</strong></p>
<p>For analysis of dissolved metal data, method accuracy and precision were assessed using the 2009 GEOTRACES coastal surface seawater (GSC) standard which produced values consistent with consensus results.</p>
<p><strong>BCO-DMO Processing Notes:&nbsp;</strong></p>
<ul>
<li>Renamed column titled yyyy-mm-dd hh:mm to Collection_date</li>
<li>Rounded latitude and longitude&nbsp;fields to 6 positions after the decimal point&nbsp;&nbsp;</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific)
iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific)
PI Supplied Instrument Name: iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific) PI Supplied Instrument Description:Following offline seaFAST preconcentration, multielemental quantitative analysis was performed using an iCAP-Q inductively coupled plasma-mass spectrometer (ICP-MS) (Thermo Scientific). Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Twelve 8L X-Niskin bottles (Ocean Test Equipment)
Twelve 8L X-Niskin bottles (Ocean Test Equipment)
PI Supplied Instrument Name: Twelve 8L X-Niskin bottles (Ocean Test Equipment) PI Supplied Instrument Description:Water samples were collected using trace metal sampling protocols described previously (Noble et al. 2012). A trace metal clean rosette suspended on a Kevlar line and equipped with twelve 8L X-Niskin bottles (Ocean Test Equipment) was used to collect seawater at depths ranging from 10 – 600 m. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Automated solid phase extraction system seaFAST-pico
Automated solid phase extraction system seaFAST-pico
PI Supplied Instrument Name: Automated solid phase extraction system seaFAST-pico PI Supplied Instrument Description:Preconcentration of spiked seawater samples for total dissolved metal analysis was performed using the automated solid phase extraction system seaFAST-pico (Elemental Scientific) in offline concentration mode with an initial volume of 15mL and elution volume of 500µL (Bown et al. 2017; Rapp et al. 2017; Jackson et al. 2018; Wuttig et al. 2019). The seaFAST contains a Nobias-chelate PA1 resin column (ethylenediaminetriacete and iminodiacetate) suitable for the simultaneous preconcentration of several trace metals (Fe, Mn, Zn, Cu, Co, Cd, Ni) with high sensitivity and quantitative recovery (Sohrin et al. 2008; Biller and Bruland 2012). Adjusted seaFAST software settings were a 17 second load loop time and a single 10mL load cycle. Process blanks consisted of pH 2 HCl (Optima, Fisher Scientific) and were processed as samples were to account for any contamination introduced by instrument processing. Instrument Name: SeaFAST Automated Preconcentration System Instrument Short Name: Instrument Description: The seaFAST is an automated sample introduction system for analysis of seawater and other high matrix samples for analyses by ICPMS (Inductively Coupled Plasma Mass Spectrometry).
Cruise: NBP1801
NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1801
Giacomo DiTullio
College of Charleston
https://service.rvdata.us/data/cruise/NBP1801/doc/NBP1801DATA.pdf
Report describing NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel