http://lod.bco-dmo.org/id/dataset/878631
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-08-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Identity of sciaenid eggs collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
2023-09-19
publication
2023-09-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-01-17
publication
https://doi.org/10.26008/1912/bco-dmo.878631.2
Lee A. Fuiman
University of Texas - Marine Science Institute
principalInvestigator
Parvathi Nair
University of Texas - Marine Science Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuiman, L. A., Nair, P. (2024) Identity of sciaenid eggs collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2023-09-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.878631.2 [access date]
Methods and Sampling: <p><strong>Sampling and analytical procedures:</strong><br />
Egg samples were collected from 20 August, 2020 through 8 March, 2022 from the research pier at the University of Texas Marine Science Institute (UTMSI), located in the Aransas Pass inlet by deploying a 500-micron mesh plankton net. Egg samples were also collected by deploying a plankton net from a small boat from a location outside the extent of the Red drum egg boon in the Aransas Bay (Mud Island) from 20 August, 2020- December 2021. Egg samples were collected bi-weekly during the Red Drum spawning season (August – December) and monthly outside the Red drum spawning season from both locations. Eggs were sorted by morphology to isolate sciaenid eggs and molecular PCR techniques were applied to subsamples of eggs to confirm the species identification.</p>
<p>DNA was extracted from a single egg using a commercial kit (Thermo scientific Gene JET genomic DNA purification kit K0721). The following cycle conditions were used to run PCRs: an initial denaturing step of 94°C for 2 minutes followed by 30 cycles of 30 seconds at 94°C; 30 s at 60°C and 1 minute at 72°C, with a final extension of 5 min at 72°C. A species-specific primer set (NADH dehydrogenase 4 mtDNA gene) was used to identify wild caught sciaenid eggs (Carreon-Martinez et al., 2010). A set of universal primers (18S ribosomal RNA gene) was used as a positive control for the DNA extraction and PCR reaction. PCR products were size separated by gel electrophoresis (1.5% agarose) and visualized by 1% ethidium bromide solution (Fisher-Scientific). The species-specific primers identified southern king fish (550 bp), gulf kingfish(400 bp), black drum (350 bp), red drum (651 bp), Atlantic croaker (850 bp), and spotted seatrout (1201 bp) using DNA ladder.</p>
<p>** Note: Eggs were not tested for black drum if egg collection date was outside the spawning season of black drum and egg size was smaller than 1.00 millimeters</p>
<p>FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0727222);&nbsp;</p>
<p>MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023618
completed
Lee A. Fuiman
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
lee.fuiman@utexas.edu
pointOfContact
Parvathi Nair
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
parvathinair@utexas.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Egg_size
Collection_date
UIN
Site
Latitude
Longitude
DNA_extraction_date
Date_analyzed
Red_Drum
Gulf_Kingfish
Southern_Kingfish
Spotted_Seatrout
Atlantic_Croaker
Black_Drum
Aria Mx Real-Time PCR System, Agilent Technologies
theme
None, User defined
length
date
sample identification
site
latitude
longitude
sample description
featureType
BCO-DMO Standard Parameters
Thermal Cycler
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
https://osprey.bco-dmo.org/project/817943
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
<p><strong>NSF Award Abstract:</strong><br />
<span style="font-size:14px"><span style="background-color:rgb(255, 255, 255)">Marine animals release extremely large numbers of eggs when they spawn. Most of these eggs are eaten by animals ranging from microscopic plankton to fish. Many egg consumers are smaller than the animals that released the eggs, representing a reversal of the usual food web. The consumption of eggs provides animals with highly nutritious molecules called essential fatty acids which are very concentrated in eggs. These essential fatty acids are important for the health of animals and the health of the whole ecosystem. When marine fishes form spawning aggregations to coordinate the timing and location of spawning, they release trillions of eggs. This results in an "egg boon" an immense but temporary concentration of highly nutritious fatty acids. This project combines field-based sampling with laboratory experiments to assess how fatty acids in the egg boons affect food webs. The project is determining whether consumption of eggs is beneficial to the condition of the egg consumers. New findings from this project are advancing the understanding of aquatic food webs and contributing to improved management of marine resources. For example, commercial harvest of fish can remove tons of fatty acids from an ecosystem by reducing egg boons and leading to cascading and unforeseen effects on those biological communities. The project is fostering the participation of women in science by substantially advancing the professional training of a female postdoctoral fellow. The project is supporting K-12 STEM education through inquiry-based and place-based programs for teachers and youth. Findings are being communicated to the public locally and nationally through participation in public lectures and contributions to the Science and the SeaTM radio program, podcast, and website.</span></span></p>
<p><span style="background-color:rgb(255, 255, 255)">Super-abundances of eggs released in temporally and spatially discrete patches create pulsed nutritional resources for egg consumers, called "egg boons", which are potentially important components of marine food webs. While various marine animals have been shown to consume eggs, the role of egg boons in energy transfer through food webs has received little attention. Three hypotheses are being tested: 1) egg boons provide a pathway through which essential fatty acids (EFAs) are redistributed counter to the main direction of trophic flow; 2) stores of EFAs in egg consumers increase during egg boons and remain elevated after the spawning season; and 3) egg boons are beneficial to the condition of egg consumers. The proposed research takes advantage of an annual egg boon produced by a spawning aggregation of the marine fish, red drum (Sciaenops ocellatus) near Port Aransas, Texas. In a combination of field sampling and laboratory experiments, fatty acid profiles, lipid content, and bulk stable isotope ratios are measures used to define trophic links between the egg boon and a selection of lower-trophic-level taxa. Egg boons are simulated in laboratory feeding experiments that are designed to enhance interpretation of data collected from field based sampling by comparing taxa that consume fish eggs with those that do not. A nucleic acid biomarker (RNA/DNA ratios) is being used to assess changes in condition that can be attributed to egg consumption in target taxa. In the environment, the importance and persistence of counter-gradient flow of fatty acids in the food web is being gauged through comparisons of samples taken inside and outside the spatial and temporal extent of the egg boon. The effects of egg consumption on consumers is being quantified in controlled experiments to identify dietary biomarkers of egg consumption in consumer tissues that can be applied to field samples. The proposed research examines how egg consumption redistributes EFAs within food webs and provides a context for considering potential controls and trophic bottlenecks that cannot be explained from the traditional element-limitation models. The integration of fatty acid and stable isotope approaches is expected to provide greater resolution for tracking organic matter through food webs and to advance the application of multi-tracer techniques in trophic investigations. Further, if egg boons are indeed an important nutritional subsidy to select groups of consumers, then subsequent studies investigating the energetic contribution of egg boons to secondary production in marine food webs are warranted. An analysis of how reduction or removal of egg resources through the harvest of fishes in spawning aggregations changes nutrient flow in food webs could have implications for ecosystem-based fisheries management.</span></p>
Egg Boon Food Webs
largerWorkCitation
project
eng; USA
oceans
-97.0727222
-97.0218
27.8396
27.9362
2020-08-21
2022-03-08
Gulf of Mexico estuary at Port Aransas, Texas
0
BCO-DMO catalogue of parameters from Identity of sciaenid eggs collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/878872.rdf
Name: Egg_size
Units: millimeters
Description: <p>Size of the egg collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/878873.rdf
Name: Collection_date
Units: unitless
Description: <p>date of egg collection in format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/878874.rdf
Name: UIN
Units: unitless
Description: <p>Unique identifiction number</p>
http://lod.bco-dmo.org/id/dataset-parameter/878875.rdf
Name: Site
Units: unitless
Description: <p>location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777))</p>
http://lod.bco-dmo.org/id/dataset-parameter/878876.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude North of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/878877.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude East (West is negative) of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/878878.rdf
Name: DNA_extraction_date
Units: unitless
Description: <p>Date DNA was extracted from egg in format YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/878879.rdf
Name: Date_analyzed
Units: unitless
Description: <p>Date PCR was run in format YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/878880.rdf
Name: Red_Drum
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as red drum based on the presence of species specific NADH band on gel electrophoresis</p>
http://lod.bco-dmo.org/id/dataset-parameter/878881.rdf
Name: Gulf_Kingfish
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as Gulf kingfish based on the presence of species specific NADH band on gel electrophoresis</p>
http://lod.bco-dmo.org/id/dataset-parameter/878882.rdf
Name: Southern_Kingfish
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as southern kingfish based on the presence of species specific NADH band on gel electrophoresis</p>
http://lod.bco-dmo.org/id/dataset-parameter/878883.rdf
Name: Spotted_Seatrout
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as spotted seatrout based on the presence of species specific NADH band on gel electrophoresis</p>
http://lod.bco-dmo.org/id/dataset-parameter/878884.rdf
Name: Atlantic_Croaker
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as atlantic croaker based on the presence of species specific NADH band on gel electrophoresis</p>
http://lod.bco-dmo.org/id/dataset-parameter/878885.rdf
Name: Black_Drum
Units: unitless
Description: <p>Eggs positively (POS) or negatively (NEG) identified as black drum based on the presence of species specific NADH band on gel electrophoresis</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
25594
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/68abc013-500d-4b8a-a868-0cb37db0fc38/content
download
https://doi.org/10.26008/1912/bco-dmo.878631.2
download
onLine
dataset
<p><strong>Sampling and analytical procedures:</strong><br />
Egg samples were collected from 20 August, 2020 through 8 March, 2022 from the research pier at the University of Texas Marine Science Institute (UTMSI), located in the Aransas Pass inlet by deploying a 500-micron mesh plankton net. Egg samples were also collected by deploying a plankton net from a small boat from a location outside the extent of the Red drum egg boon in the Aransas Bay (Mud Island) from 20 August, 2020- December 2021. Egg samples were collected bi-weekly during the Red Drum spawning season (August – December) and monthly outside the Red drum spawning season from both locations. Eggs were sorted by morphology to isolate sciaenid eggs and molecular PCR techniques were applied to subsamples of eggs to confirm the species identification.</p>
<p>DNA was extracted from a single egg using a commercial kit (Thermo scientific Gene JET genomic DNA purification kit K0721). The following cycle conditions were used to run PCRs: an initial denaturing step of 94°C for 2 minutes followed by 30 cycles of 30 seconds at 94°C; 30 s at 60°C and 1 minute at 72°C, with a final extension of 5 min at 72°C. A species-specific primer set (NADH dehydrogenase 4 mtDNA gene) was used to identify wild caught sciaenid eggs (Carreon-Martinez et al., 2010). A set of universal primers (18S ribosomal RNA gene) was used as a positive control for the DNA extraction and PCR reaction. PCR products were size separated by gel electrophoresis (1.5% agarose) and visualized by 1% ethidium bromide solution (Fisher-Scientific). The species-specific primers identified southern king fish (550 bp), gulf kingfish(400 bp), black drum (350 bp), red drum (651 bp), Atlantic croaker (850 bp), and spotted seatrout (1201 bp) using DNA ladder.</p>
<p>** Note: Eggs were not tested for black drum if egg collection date was outside the spawning season of black drum and egg size was smaller than 1.00 millimeters</p>
<p>FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0727222);&nbsp;</p>
<p>MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).</p>
Specified by the Principal Investigator(s)
<p><strong>Dataset version 1 (2022-12-01):</strong><br />
- File&nbsp;"Egg PCR Final Data 2020-2022.xlsx" (submitted 2022-07-08 by email &nbsp;)&nbsp; was imported into the bco-dmo data system.<br />
- Converted dates to format (YYYY-MM-DD)<br />
- Adjusted field/parameter names to comply with BCO-DMO naming conventions<br />
- Added columns for Latitude and Longitude<br />
- Added a conventional header with dataset name, PI names, version date<br />
<br />
<strong>Dataset version 2 (2023-09-19):</strong><br />
* Sheet 1 of file "Egg PCR Final Data&nbsp; 2020-2022.xlsx" (submitted by email Jun 22, 2023)&nbsp; was imported into the BCO-DMO data system.&nbsp;&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Aria Mx Real-Time PCR System, Agilent Technologies
Aria Mx Real-Time PCR System, Agilent Technologies
PI Supplied Instrument Name: Aria Mx Real-Time PCR System, Agilent Technologies Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)