http://lod.bco-dmo.org/id/dataset/878635
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-08-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fatty acid measurements for animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
2023-09-19
publication
2023-09-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-01-16
publication
https://doi.org/10.26008/1912/bco-dmo.878635.1
Lee A. Fuiman
University of Texas - Marine Science Institute
principalInvestigator
Parvathi Nair
University of Texas - Marine Science Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuiman, L. A., Nair, P. (2024) Fatty acid measurements for animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.878635.1 [access date]
Dataset Description: <p>These data were published in Nair et al. (2023).</p> Methods and Sampling: Sampling and analytical procedures:
Mnemiopsis leidyi and juvenile Callinectes similis were collected in March – April 2022, and Beroe ovata was collected in November 2022, using a plankton net (50 cm diameter, 500 μm mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W). Palaemonetes pugio was collected using a plankton net from Corpus Christi Bay (27.8035° N, 97.0898° W) in Port Aransas in July 2021. Opisthonema oglinum and Lagodon rhomboides were collected using a seine (6.4 m wide by 1.2 m high with 5 mm square mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W) in August 2021 and Redfish Bay at Aransas Pass (27.8611° N, 97.07632° W) in May 2022, respectively.
The live animals of each species were divided into two treatments (control and experimental). Both treatments were fed a common diet of either live Artemia sp. nauplii (enriched with Alga-Mac 3050; Aquafauna Bio-Marine, Inc.) or commercial fish food, Otohime (EP1, Reed Mariculture, Inc.) during the acclimation period of 10 – 45 days. After acclimation (Day 0), both treatments received a common diet of Artemia or Otohime, and the diet of experimental treatments was supplemented with red drum eggs for a period of 10 – 94 days. Controls did not receive eggs. Three to eight tanks of study species were sampled at the end of acclimation (day 0). Three to eight replicate tanks (N) were sampled from each treatment 24 h and 2 – 5 days after the experimental treatment received eggs.
Mnemiopsis leidyi, B. ovata, and C. similis were held in rectangular tanks (26.7 cm long x 16.5 cm high x 16.5 cm wide), and P. pugio and fishes were held in circular tanks (106.7 cm in diameter, 43.2 cm deep) with recirculating filtered water. Within each rectangular tank, individuals of C. similis were held eparately in round plastic containers (12 cm in diameter, 6.4 cm deep) with perforated lids to prevent aggressive contact. For the same reason, individuals of L. rhomboides were kept in separate perforated cylindrical enclosures (30 cm in diameter, 45 cm high) within each circular tank. Excess food and solid waste were siphoned daily from all tanks, and complete water changes were performed in rectangular tanks every 2 – 4 days. Environmental conditions were measured daily and were constant throughout the experiment (temperature: 21 – 24°C, salinity: 28 – 35 ppt, and photoperiod: 12-h light and 12-h dark).
Invertebrates removed from both treatments on sampling days were kept in clean sea water overnight to evacuate their guts and were sacrificed the following morning. For taxa with low dry weight, i.e., ctenophores, 3 – 4 individuals from each tank were pooled together to make a replicate. A single individual per tank of C. similis, and three individuals of P. pugio (subsamples, n=3) per tank were removed at each sampling day. Invertebrates were analyzed whole, except for C. similis, for which the exoskeleton was excluded. On each sampling day, one fish per tank was removed and immediately euthanized with tricaine methanesulfonate (MS-222). Euthanized fish were placed on ice where the liver and a fillet of dorsal white muscle tissue were collected. Subsamples (n = 5 – 21) of diets provided to both treatments (i.e., red drum eggs, Artemia, and Otohime) were collected throughout the experiments for each taxon. All samples were rinsed twice in distilled water and frozen at -80°C until analysis.
Samples were lyophilized, homogenized, and weighed. Fatty acid profiles for all samples were obtained following established procedures by Faulk and Holt, (2005). Lipids were cold-extracted from each sample by homogenizing in a chloroform-methanol solution (2:1 volume:volume). Fatty acid methyl esters (FAMEs) were prepared by saponification in 0.5 M potassium hydroxide, followed by 14% boron trifluoride in methanol. Fatty acid methyl esters were analyzed using a Shimadzu GC-2014 gas chromatograph (GC) with a flame ionization detector (GC-FID; Shimadzu Scientific Instruments, Columbia, MD, USA). Individual fatty acids were identified by comparison to commercial FAME standards (marine PUFA no. 3, Bacterial and Supelco 37 component FAMEs mix; Sigma-Aldrich, St. Louis, MO, USA). Thirty four FAs were measured on every sample. Measurements were expressed in terms of concentration (mg per g dry weight) and composition (% of total fatty acids).
Laboratory experiments took place at the Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute from July 2021 to November 2022.
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023618
completed
Lee A. Fuiman
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
lee.fuiman@utexas.edu
pointOfContact
Parvathi Nair
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
parvathinair@utexas.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Taxon
Tissue_sampled
Length
Tank_number
Acclimation_days
Days_after_acclimation
Treatment
Diet_fed
Notes
Primary_check
C14_0_mg
C15_0_mg
C16_0_mg
C16_1n7_mg
C16_2n4_mg
C17_0_mg
C16_3n4_mg
C17_1_mg
C18_0_mg
C18_1n9_mg
C18_1n7_mg
C18_2n6_mg
C18_3n6_mg
C18_3n4_mg
C18_3n3_mg
C18_4n3_mg
C20_1n9_mg
C20_2n6_mg
C20_3n6_mg
C20_4n6_mg
C20_3n3_mg
C20_4n3_mg
C20_5n3_mg
C22_1n11_mg
C22_4n6_mg
C22_5n6_mg
C22_5n3_mg
C22_6n3_mg
C20_0_mg
Ci_15_0_mg
C14_1_mg
Ci_17_0_mg
Ca_15_0_mg
Ci_16_0_mg
C14_0_pct
C15_0_pct
C16_0_pct
C16_1n7_pct
C16_2n4_pct
C17_0_pct
C16_3n4_pct
C17_1_pct
C18_0_pct
C18_1n9_pct
C18_1n7_pct
C18_2n6_pct
C18_3n6_pct
C18_3n4_pct
C18_3n3_pct
C18_4n3_pct
C20_1n9_pct
C20_2n6_pct
C20_3n6_pct
C20_4n6_pct
C20_3n3_pct
C20_4n3_pct
C20_5n3_pct
C22_1n11_pct
C22_4n6_pct
C22_5n6_pct
C22_5n3_pct
C22_6n3_pct
C20_0_pct
Ci_15_0_pct
C14_1_pct
Ci_17_0_pct
Ca_15_0_pct
Ci_16_0_pct
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
theme
None, User defined
species scientific name (binomial)
sample description
length
time_elapsed
treatment
comments
quality flag
lipids in biota
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
https://osprey.bco-dmo.org/project/817943
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
<p><strong>NSF Award Abstract:</strong><br />
<span style="font-size:14px"><span style="background-color:rgb(255, 255, 255)">Marine animals release extremely large numbers of eggs when they spawn. Most of these eggs are eaten by animals ranging from microscopic plankton to fish. Many egg consumers are smaller than the animals that released the eggs, representing a reversal of the usual food web. The consumption of eggs provides animals with highly nutritious molecules called essential fatty acids which are very concentrated in eggs. These essential fatty acids are important for the health of animals and the health of the whole ecosystem. When marine fishes form spawning aggregations to coordinate the timing and location of spawning, they release trillions of eggs. This results in an "egg boon" an immense but temporary concentration of highly nutritious fatty acids. This project combines field-based sampling with laboratory experiments to assess how fatty acids in the egg boons affect food webs. The project is determining whether consumption of eggs is beneficial to the condition of the egg consumers. New findings from this project are advancing the understanding of aquatic food webs and contributing to improved management of marine resources. For example, commercial harvest of fish can remove tons of fatty acids from an ecosystem by reducing egg boons and leading to cascading and unforeseen effects on those biological communities. The project is fostering the participation of women in science by substantially advancing the professional training of a female postdoctoral fellow. The project is supporting K-12 STEM education through inquiry-based and place-based programs for teachers and youth. Findings are being communicated to the public locally and nationally through participation in public lectures and contributions to the Science and the SeaTM radio program, podcast, and website.</span></span></p>
<p><span style="background-color:rgb(255, 255, 255)">Super-abundances of eggs released in temporally and spatially discrete patches create pulsed nutritional resources for egg consumers, called "egg boons", which are potentially important components of marine food webs. While various marine animals have been shown to consume eggs, the role of egg boons in energy transfer through food webs has received little attention. Three hypotheses are being tested: 1) egg boons provide a pathway through which essential fatty acids (EFAs) are redistributed counter to the main direction of trophic flow; 2) stores of EFAs in egg consumers increase during egg boons and remain elevated after the spawning season; and 3) egg boons are beneficial to the condition of egg consumers. The proposed research takes advantage of an annual egg boon produced by a spawning aggregation of the marine fish, red drum (Sciaenops ocellatus) near Port Aransas, Texas. In a combination of field sampling and laboratory experiments, fatty acid profiles, lipid content, and bulk stable isotope ratios are measures used to define trophic links between the egg boon and a selection of lower-trophic-level taxa. Egg boons are simulated in laboratory feeding experiments that are designed to enhance interpretation of data collected from field based sampling by comparing taxa that consume fish eggs with those that do not. A nucleic acid biomarker (RNA/DNA ratios) is being used to assess changes in condition that can be attributed to egg consumption in target taxa. In the environment, the importance and persistence of counter-gradient flow of fatty acids in the food web is being gauged through comparisons of samples taken inside and outside the spatial and temporal extent of the egg boon. The effects of egg consumption on consumers is being quantified in controlled experiments to identify dietary biomarkers of egg consumption in consumer tissues that can be applied to field samples. The proposed research examines how egg consumption redistributes EFAs within food webs and provides a context for considering potential controls and trophic bottlenecks that cannot be explained from the traditional element-limitation models. The integration of fatty acid and stable isotope approaches is expected to provide greater resolution for tracking organic matter through food webs and to advance the application of multi-tracer techniques in trophic investigations. Further, if egg boons are indeed an important nutritional subsidy to select groups of consumers, then subsequent studies investigating the energetic contribution of egg boons to secondary production in marine food webs are warranted. An analysis of how reduction or removal of egg resources through the harvest of fishes in spawning aggregations changes nutrient flow in food webs could have implications for ecosystem-based fisheries management.</span></p>
Egg Boon Food Webs
largerWorkCitation
project
eng; USA
oceans
-97.0898
-97.0726
27.8035
27.8611
2020-07-01
2022-11-01
Gulf of Mexico estuary at Port Aransas, Texas
0
BCO-DMO catalogue of parameters from Fatty acid measurements for animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/916296.rdf
Name: Taxon
Units: unitless
Description: <p>Taxonomic grouping of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/916297.rdf
Name: Tissue_sampled
Units: unitless
Description: <p>Animal tissue sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/916298.rdf
Name: Length
Units: cm
Description: <p>Total length of fish; carapace length of crabs</p>
http://lod.bco-dmo.org/id/dataset-parameter/916299.rdf
Name: Tank_number
Units: unitless
Description: <p>Tank that animal was assigned to</p>
http://lod.bco-dmo.org/id/dataset-parameter/916300.rdf
Name: Acclimation_days
Units: days
Description: <p>Acclimation period began soon after animals were collected from the wild. During acclimation animals were fed Artemia or Otohime</p>
http://lod.bco-dmo.org/id/dataset-parameter/916301.rdf
Name: Days_after_acclimation
Units: days
Description: <p>Days in control or experimental treatment after acclimation. End of Acclimation marked by Day 0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916302.rdf
Name: Treatment
Units: unitless
Description: <p>Control or Experimental</p>
http://lod.bco-dmo.org/id/dataset-parameter/916303.rdf
Name: Diet_fed
Units: unitless
Description: <p>Diets provided to control and experimental treatment. Controls were fed Artemia/Otohime only. Experimentals were fed Artemia/Otohime supplemented with red drum eggs</p>
http://lod.bco-dmo.org/id/dataset-parameter/916304.rdf
Name: Notes
Units: unitless
Description: <p>Notes about sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/916305.rdf
Name: Primary_check
Units: unitless
Description: <p>Primary QC check (1=perfectly fine)</p>
http://lod.bco-dmo.org/id/dataset-parameter/916306.rdf
Name: C14_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 14:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916307.rdf
Name: C15_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916308.rdf
Name: C16_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916309.rdf
Name: C16_1n7_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:1n-7</p>
http://lod.bco-dmo.org/id/dataset-parameter/916310.rdf
Name: C16_2n4_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:2n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916311.rdf
Name: C17_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 17:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916312.rdf
Name: C16_3n4_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:3n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916313.rdf
Name: C17_1_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 17:1</p>
http://lod.bco-dmo.org/id/dataset-parameter/916314.rdf
Name: C18_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916315.rdf
Name: C18_1n9_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:1n-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/916316.rdf
Name: C18_1n7_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:1n-7</p>
http://lod.bco-dmo.org/id/dataset-parameter/916317.rdf
Name: C18_2n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:2n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916318.rdf
Name: C18_3n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916319.rdf
Name: C18_3n4_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916320.rdf
Name: C18_3n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916321.rdf
Name: C18_4n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:4n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916322.rdf
Name: C20_1n9_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:1n-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/916323.rdf
Name: C20_2n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:2n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916324.rdf
Name: C20_3n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:3n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916325.rdf
Name: C20_4n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:4n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916326.rdf
Name: C20_3n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:3n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916327.rdf
Name: C20_4n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:4n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916328.rdf
Name: C20_5n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:5n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916329.rdf
Name: C22_1n11_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:1n-11</p>
http://lod.bco-dmo.org/id/dataset-parameter/916330.rdf
Name: C22_4n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:4n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916331.rdf
Name: C22_5n6_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:5n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916332.rdf
Name: C22_5n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:5n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916333.rdf
Name: C22_6n3_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:6n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916334.rdf
Name: C20_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916335.rdf
Name: Ci_15_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso- 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916336.rdf
Name: C14_1_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 14:1</p>
http://lod.bco-dmo.org/id/dataset-parameter/916337.rdf
Name: Ci_17_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso- 17:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916338.rdf
Name: Ca_15_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid anteiso- 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916339.rdf
Name: Ci_16_0_mg
Units: milligrams per gram of dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso- 16:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916340.rdf
Name: C14_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 14:0.</p>
http://lod.bco-dmo.org/id/dataset-parameter/916341.rdf
Name: C15_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916342.rdf
Name: C16_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 16:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916343.rdf
Name: C16_1n7_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 16:1n-7</p>
http://lod.bco-dmo.org/id/dataset-parameter/916344.rdf
Name: C16_2n4_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 16:2n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916345.rdf
Name: C17_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 17:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916346.rdf
Name: C16_3n4_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 16:3n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916347.rdf
Name: C17_1_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 17:1</p>
http://lod.bco-dmo.org/id/dataset-parameter/916348.rdf
Name: C18_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916349.rdf
Name: C18_1n9_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:1n-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/916350.rdf
Name: C18_1n7_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:1n-7</p>
http://lod.bco-dmo.org/id/dataset-parameter/916351.rdf
Name: C18_2n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:2n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916352.rdf
Name: C18_3n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:3n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916353.rdf
Name: C18_3n4_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:3n-4</p>
http://lod.bco-dmo.org/id/dataset-parameter/916354.rdf
Name: C18_3n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:3n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916355.rdf
Name: C18_4n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 18:4n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916356.rdf
Name: C20_1n9_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:1n-9</p>
http://lod.bco-dmo.org/id/dataset-parameter/916357.rdf
Name: C20_2n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:2n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916358.rdf
Name: C20_3n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:3n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916359.rdf
Name: C20_4n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:4n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916360.rdf
Name: C20_3n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:3n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916361.rdf
Name: C20_4n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:4n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916362.rdf
Name: C20_5n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:5n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916363.rdf
Name: C22_1n11_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 22:1n-11</p>
http://lod.bco-dmo.org/id/dataset-parameter/916364.rdf
Name: C22_4n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 22:4n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916365.rdf
Name: C22_5n6_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 22:5n-6</p>
http://lod.bco-dmo.org/id/dataset-parameter/916366.rdf
Name: C22_5n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 22:5n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916367.rdf
Name: C22_6n3_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 22:6n-3</p>
http://lod.bco-dmo.org/id/dataset-parameter/916368.rdf
Name: C20_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 20:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916369.rdf
Name: Ci_15_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid iso- 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916370.rdf
Name: C14_1_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid 14:1</p>
http://lod.bco-dmo.org/id/dataset-parameter/916371.rdf
Name: Ci_17_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid iso- 17:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916372.rdf
Name: Ca_15_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid anteiso- 15:0</p>
http://lod.bco-dmo.org/id/dataset-parameter/916373.rdf
Name: Ci_16_0_pct
Units: percent total fatty acids (%)
Description: <p>measured value of fatty acid iso- 16:0</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
170563
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/e97b9b9a-abc1-4ca2-9021-3c2574f2091a/content
download
https://doi.org/10.26008/1912/bco-dmo.878635.1
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onLine
dataset
Sampling and analytical procedures:
Mnemiopsis leidyi and juvenile Callinectes similis were collected in March – April 2022, and Beroe ovata was collected in November 2022, using a plankton net (50 cm diameter, 500 μm mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W). Palaemonetes pugio was collected using a plankton net from Corpus Christi Bay (27.8035° N, 97.0898° W) in Port Aransas in July 2021. Opisthonema oglinum and Lagodon rhomboides were collected using a seine (6.4 m wide by 1.2 m high with 5 mm square mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W) in August 2021 and Redfish Bay at Aransas Pass (27.8611° N, 97.07632° W) in May 2022, respectively.
The live animals of each species were divided into two treatments (control and experimental). Both treatments were fed a common diet of either live Artemia sp. nauplii (enriched with Alga-Mac 3050; Aquafauna Bio-Marine, Inc.) or commercial fish food, Otohime (EP1, Reed Mariculture, Inc.) during the acclimation period of 10 – 45 days. After acclimation (Day 0), both treatments received a common diet of Artemia or Otohime, and the diet of experimental treatments was supplemented with red drum eggs for a period of 10 – 94 days. Controls did not receive eggs. Three to eight tanks of study species were sampled at the end of acclimation (day 0). Three to eight replicate tanks (N) were sampled from each treatment 24 h and 2 – 5 days after the experimental treatment received eggs.
Mnemiopsis leidyi, B. ovata, and C. similis were held in rectangular tanks (26.7 cm long x 16.5 cm high x 16.5 cm wide), and P. pugio and fishes were held in circular tanks (106.7 cm in diameter, 43.2 cm deep) with recirculating filtered water. Within each rectangular tank, individuals of C. similis were held eparately in round plastic containers (12 cm in diameter, 6.4 cm deep) with perforated lids to prevent aggressive contact. For the same reason, individuals of L. rhomboides were kept in separate perforated cylindrical enclosures (30 cm in diameter, 45 cm high) within each circular tank. Excess food and solid waste were siphoned daily from all tanks, and complete water changes were performed in rectangular tanks every 2 – 4 days. Environmental conditions were measured daily and were constant throughout the experiment (temperature: 21 – 24°C, salinity: 28 – 35 ppt, and photoperiod: 12-h light and 12-h dark).
Invertebrates removed from both treatments on sampling days were kept in clean sea water overnight to evacuate their guts and were sacrificed the following morning. For taxa with low dry weight, i.e., ctenophores, 3 – 4 individuals from each tank were pooled together to make a replicate. A single individual per tank of C. similis, and three individuals of P. pugio (subsamples, n=3) per tank were removed at each sampling day. Invertebrates were analyzed whole, except for C. similis, for which the exoskeleton was excluded. On each sampling day, one fish per tank was removed and immediately euthanized with tricaine methanesulfonate (MS-222). Euthanized fish were placed on ice where the liver and a fillet of dorsal white muscle tissue were collected. Subsamples (n = 5 – 21) of diets provided to both treatments (i.e., red drum eggs, Artemia, and Otohime) were collected throughout the experiments for each taxon. All samples were rinsed twice in distilled water and frozen at -80°C until analysis.
Samples were lyophilized, homogenized, and weighed. Fatty acid profiles for all samples were obtained following established procedures by Faulk and Holt, (2005). Lipids were cold-extracted from each sample by homogenizing in a chloroform-methanol solution (2:1 volume:volume). Fatty acid methyl esters (FAMEs) were prepared by saponification in 0.5 M potassium hydroxide, followed by 14% boron trifluoride in methanol. Fatty acid methyl esters were analyzed using a Shimadzu GC-2014 gas chromatograph (GC) with a flame ionization detector (GC-FID; Shimadzu Scientific Instruments, Columbia, MD, USA). Individual fatty acids were identified by comparison to commercial FAME standards (marine PUFA no. 3, Bacterial and Supelco 37 component FAMEs mix; Sigma-Aldrich, St. Louis, MO, USA). Thirty four FAs were measured on every sample. Measurements were expressed in terms of concentration (mg per g dry weight) and composition (% of total fatty acids).
Laboratory experiments took place at the Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute from July 2021 to November 2022.
Specified by the Principal Investigator(s)
Identified peaks from the gas chromatograms were quantified using commercial FAME standards, such as Supelco 37 Component FAME mix, polyunsaturated fatty acid mix no. 3 (PUFA-3), and bacterial acid methyl ester mix (BAME).
Quality control procedure:
Principal components analysis for each taxon was performed on the mg g-1 dw and percent total fatty acids data, separately. Individual samples for which the score on the first or second principal component axis were greater than 4 standard deviations from the taxon mean were removed from the data set.
A primary check value was assigned in the data column "Primary_check" as follows:
1 = Perfectly fine
The quantification limit for individual fatty acids is 0.00044 mg g-1 dry weight
Measured values of any fatty acid less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for the percentage of total fatty acids was changed to 0.0%
Specified by the Principal Investigator(s)
BCO-DMO Data Manager Processing Notes:
* Sheet 1 of file "FA profile of experimentals.xlsx" (submitted in our online submission system 2023-06-23) was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Taxon name and associated LSID for names in fatty acid measurement dataset 878635 version 1 as of 2023-06-23 (source: World Register of Marine Species). Added this list to Methods and Sampling section.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
PI Supplied Instrument Name: Shimadzu GC-2014 gas chromatograph with a flame ionization detector Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/