http://lod.bco-dmo.org/id/dataset/879076
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-08-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line I07N RB1803 in the Western Indian Ocean from April to June 2018 (Ocean Stoichiometry Project)
2022-08-24
publication
2022-08-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-09-28
publication
https://doi.org/10.26008/1912/bco-dmo.879076.1
Adam Martiny
University of California-Irvine
principalInvestigator
Catherine Garcia
University of California-Irvine
principalInvestigator
Allison R. Moreno
University of California-Irvine
principalInvestigator
Tatsuro Tanioka
University of California-Irvine
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Martiny, A., Garcia, C., Moreno, A. R., Tanioka, T. (2022) POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line I07N RB1803 in the Western Indian Ocean from April to June 2018 (Ocean Stoichiometry Project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-08-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.879076.1 [access date]
POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line I07N in 2018 Dataset Description: <p>The operating area was in the western Indian Ocean. The I07N section runs across the Madagascar and Mascarene Basins in the south, crosses the Amirante Trench, and after the Seychelles Bank it crosses the Somalia Basin, Carlsberg Ridge, and the Arabian Sea in the north.&nbsp;</p> Methods and Sampling: <p><strong>POM sample collection:</strong><br />
General equipment preparation in the lab before shipment included an HCl bath (1.0 M HCl overnight) and milli-Q rinse for the 8 L carboys, tubing, and filter holders. Additionally, the carboys were autoclaved. The 25 mm, 0.7 μm GF/F filters and aluminum foil used to wrap the filters were combusted (500 °C for 5 hours) in aluminum foil packets to remove any traces of carbon present. The forceps that came in contact with the samples were wiped with 70% ethanol before and between uses. Seawater for the POM samples was collected from the onboard flow through the underway system.&nbsp;&nbsp;Before sampling, the carboys used were rinsed twice with the pre-filtered underway seawater.&nbsp;</p>
<p>POM samples smaller&nbsp;than &lt; 30µmwere collected hourly and at noon samples were collected in triplicate.&nbsp;&nbsp;30 μm nylon mesh pre-filter was attached to the underway outlet for all standard samples to separate and remove&nbsp;POM samples larger than &gt; 30µm (e.g.,&nbsp;large plankton and particulates).&nbsp;After filtration, all POP triplicates were rinsed with approximately 2-5 mL of a 0.17 M Na2SO4 solution to remove dissolved phosphorus.&nbsp;</p>
<p>POM samples larger than &gt; 30µm were collected on nitex mesh for ~1 hour. The flow&nbsp;rate was measured by measuring volume in an 8L carboy over a duration of several minutes. Mesh was rinsed into a container with filter seawater and then split onto duplicate GF/F samples for POC/PON.&nbsp;</p>
<p>All POM samples were folded in half with the top sides toward each other, sealed inside pieces of precombusted aluminum foil, and stored in a -20&nbsp;°C&nbsp;freezer until analysis.</p>
<p><strong>POP assay:</strong></p>
<p>The POP data were obtained using an ash/hydrolysis method and comparing the samples to a set of standard phosphorus concentrations (Lomas et al., 2010,&nbsp;<em>Biogeosciences</em>,&nbsp;<strong>7</strong>(2), 695-710, doi:10.5194/bg-7-695-2010). The sample filters were unfolded and placed face up into acid-bathed and combusted scintillation vials. Along with each set of samples, 10 different volumes (ranging 0 - 0.5 mL) of 0.1 M KH2PO4 solution were added to scintillation vials. 2 mL of a 0.017 M MgSO4 drying solution was added to each scintillation vial and then all vials were placed into an 80-90 °C oven overnight to dry. After drying, the vials were heated at 500 °C for 2 hours, then left to cool before adding 5 mL of 0.2 M HCl to each vial and being returned to the 80-90&nbsp;°C&nbsp;oven for 30 minutes after being brought up to temperature. The solutions were then transferred to 15 mL glass centrifuge tubes (prepared in the same way as the scintillation vials). The sample vials were rinsed with 5 mL of milli-Q each, which was also added to the centrifuge tubes. 1 mL of a mixed reagent containing 2:5:1:2 parts Ammonium Molybdate ((NH4)6Mo7O24), 5.0 N H2SO4, Potassium Antimonyl Tartrate (C8H4K2O12Sb2), and Ascorbic Acid (C6H8O6) respectively was added to each centrifuge tube in 30-second intervals. Each of the sample tubes was centrifuged to isolate any glass fibers that could interfere with the absorbance reading. Lastly, after allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements.</p>
<p><strong>POC/PON assay:</strong><br />
POC and PON measurements were analyzed both in-lab at UCI. The preparation for all samples was the same; the samples were each removed from their foil packets and placed into acid-bathed and combusted scintillation vials and dried in a 55 °C oven overnight. The scintillation vials were then placed in a desiccator containing a beaker of 12 M HCl overnight before being dried at 55 °C at least overnight once more. Samples sent to UCSB were then capped and shipped to the lab. Samples analyzed at UCI were packed into tin packets alongside Atropine (C17H23NO3) standards (ranging from 0.2-1.5 mg) and measured using a Flash EA elemental analyzer.</p>
<p>Associated GO-SHIP I07N underway and bottle datasets can be found on the CLIVAR and Carbon Hydrographic Data Office Section I07N homepage:&nbsp;https://cchdo.ucsd.edu/cruise/33RO20180423.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1848576 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1848576
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Catherine Garcia
University of California-Irvine
cathy.garcia@hawaii.edu
pointOfContact
Allison R. Moreno
University of California-Irvine
UCLA Atmospheric & Oceanic Sciences
Los Angeles
CA
USA
allimoreno@atmos.ucla.edu
pointOfContact
Tatsuro Tanioka
University of California-Irvine
Irvine
CA
USA
tatsurt@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Station
Latitude
Longitude
ISO_DateTime_UTC
Datenum
Replicate
Vol_CN_L
Vol_POP_L
Vol_largeCN_L
POC_uM
PON_uM
POP_uM
POC_large_umol
PON_large_umol
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
theme
None, User defined
sample identification
station id
latitude
longitude
ISO_DateTime_UTC
days
replicate
volume
particulate organic Carbon (POC)
particulate organic nitrogen
particulate organic phosphorus
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
RB1803
service
Deployment Activity
Western Indian Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
https://osprey.bco-dmo.org/project/764270
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
<p><em>NSF Award Abstract:</em><br />
Due to their sheer abundance and high activity, microorganisms have the potential to greatly influence how ecosystems are affected by changes in their environment. However, descriptions of microbial physiology and diversity are local and highly complex and thus rarely considered in Earth System Models. Thus, the researchers focus on a convergence research framework that can qualitatively and quantitatively integrate eco-evolutionary changes in microorganisms with global biogeochemistry. Here, the investigators will develop an approach that integrates the knowledge and tools of biologists, mathematicians, engineers, and geoscientists to understand the link between the ocean nutrient and carbon cycles. The integration of data and knowledge from diverse fields will provide a robust, biologically rich, and computationally efficient prediction for the variation in plankton resource requirements and the biogeochemical implications, addressing a fundamental challenge in ocean science. In addition, the project can serve as a road map for many other research groups facing a similar lack of convergence between biology and geoscience.</p>
<p>Traditionally, the cellular elemental ratios of Carbon, Nitrogen, and Phosphorus (C:N:P) of marine communities have been considered static at Redfield proportions but recent studies have demonstrated strong latitudinal variation. Such regional variation may have large - but poorly constrained - implications for marine biodiversity, biogeochemical functioning, and atmospheric carbon dioxide levels. As such, variations in ocean community C:N:P may represent an important biological feedback. Here, the investigators propose a convergence research framework integrating cellular and ecological processes controlling microbial resource allocations with an Earth System model. The approach combines culture experiments and omics measurements to provide a molecular understanding of cellular resource allocations. Using a mathematical framework of increasing complexity describing communicating, moving demes, the team will quantify the extent to which local mixing, environmental heterogeneity and evolution lead to systematic deviations in plankton resource allocations and C:N:P. Optimization tools from engineering science will be used to facilitate the quantitative integration of models and observations across a range of scales and complexity levels. Finally, global ocean modeling will enable understanding of how plankton resource use impacts Earth System processes. By integrating data and knowledge across fields, scales and complexity, the investigators will develop a robust link between variation in plankton C:N:P and global biogeochemical cycles.</p>
Ocean Stoichiometry
largerWorkCitation
project
eng; USA
oceans
Western Indian Ocean
40.0075
69.4832
-30.0118
17.9987
2018-04-25
2018-06-04
0
BCO-DMO catalogue of parameters from POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line I07N RB1803 in the Western Indian Ocean from April to June 2018 (Ocean Stoichiometry Project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/880799.rdf
Name: Sample
Units: unitless
Description: <p>sample numbers 0-790</p>
http://lod.bco-dmo.org/id/dataset-parameter/880800.rdf
Name: Station
Units: unitless
Description: <p>stations for cruise I07_###</p>
http://lod.bco-dmo.org/id/dataset-parameter/880801.rdf
Name: Latitude
Units: decimal degrees
Description: <p>negative values indicate West</p>
http://lod.bco-dmo.org/id/dataset-parameter/880802.rdf
Name: Longitude
Units: decimal degrees
Description: <p>positive values indicate North</p>
http://lod.bco-dmo.org/id/dataset-parameter/880803.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>datetime in UTC; format: %Y-%m-%dT%H:%MZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/880804.rdf
Name: Datenum
Units: days
Description: <p>days since Jan-0-0000</p>
http://lod.bco-dmo.org/id/dataset-parameter/880805.rdf
Name: Replicate
Units: replicates
Description: <p>replicates collected at noon</p>
http://lod.bco-dmo.org/id/dataset-parameter/880806.rdf
Name: Vol_CN_L
Units: liters
Description: <p>seawater volume in liters for POC and PON sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/880807.rdf
Name: Vol_POP_L
Units: liters
Description: <p>seawater volume in liters for POP sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/880808.rdf
Name: Vol_largeCN_L
Units: liters
Description: <p>seawater volume for large POC and PON sample, calculated from Flowrate (L/min) and time</p>
http://lod.bco-dmo.org/id/dataset-parameter/880809.rdf
Name: POC_uM
Units: micromoles per liter (µM)
Description: <p>particulate organic carbon, underway seawater collected on 25mm GF/F with 30 µm nitex mesh prefilter</p>
http://lod.bco-dmo.org/id/dataset-parameter/880810.rdf
Name: PON_uM
Units: micromoles per liter (µM)
Description: <p>particulate organic nitrogen, underway seawater collected on 25mm GF/F with 30 µm nitex mesh prefilter</p>
http://lod.bco-dmo.org/id/dataset-parameter/880811.rdf
Name: POP_uM
Units: micromoles per liter (µM)
Description: <p>particulate organic phosphorus, underway seawater collected on 25mm GF/F with 30 µm nitex mesh prefilter</p>
http://lod.bco-dmo.org/id/dataset-parameter/880812.rdf
Name: POC_large_umol
Units: micromoles per liter (µM)
Description: <p>particulate organic carbon, underway seawater size-fractionated above 30 µm nitex mesh for ~1 hour and then filtered onto GF/F</p>
http://lod.bco-dmo.org/id/dataset-parameter/880813.rdf
Name: PON_large_umol
Units: micromoles per liter (µM)
Description: <p>particulate organic nitrogen, underway seawater size-fractionated above 30 µm nitex mesh for ~1 hour and then filtered onto GF/F</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
81663
https://darchive.mblwhoilibrary.org/bitstream/1912/29374/1/dataset-879076_go-ship-line-i07n-pom-concentrations__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.879076.1
download
onLine
dataset
<p><strong>POM sample collection:</strong><br />
General equipment preparation in the lab before shipment included an HCl bath (1.0 M HCl overnight) and milli-Q rinse for the 8 L carboys, tubing, and filter holders. Additionally, the carboys were autoclaved. The 25 mm, 0.7 μm GF/F filters and aluminum foil used to wrap the filters were combusted (500 °C for 5 hours) in aluminum foil packets to remove any traces of carbon present. The forceps that came in contact with the samples were wiped with 70% ethanol before and between uses. Seawater for the POM samples was collected from the onboard flow through the underway system.&nbsp;&nbsp;Before sampling, the carboys used were rinsed twice with the pre-filtered underway seawater.&nbsp;</p>
<p>POM samples smaller&nbsp;than &lt; 30µmwere collected hourly and at noon samples were collected in triplicate.&nbsp;&nbsp;30 μm nylon mesh pre-filter was attached to the underway outlet for all standard samples to separate and remove&nbsp;POM samples larger than &gt; 30µm (e.g.,&nbsp;large plankton and particulates).&nbsp;After filtration, all POP triplicates were rinsed with approximately 2-5 mL of a 0.17 M Na2SO4 solution to remove dissolved phosphorus.&nbsp;</p>
<p>POM samples larger than &gt; 30µm were collected on nitex mesh for ~1 hour. The flow&nbsp;rate was measured by measuring volume in an 8L carboy over a duration of several minutes. Mesh was rinsed into a container with filter seawater and then split onto duplicate GF/F samples for POC/PON.&nbsp;</p>
<p>All POM samples were folded in half with the top sides toward each other, sealed inside pieces of precombusted aluminum foil, and stored in a -20&nbsp;°C&nbsp;freezer until analysis.</p>
<p><strong>POP assay:</strong></p>
<p>The POP data were obtained using an ash/hydrolysis method and comparing the samples to a set of standard phosphorus concentrations (Lomas et al., 2010,&nbsp;<em>Biogeosciences</em>,&nbsp;<strong>7</strong>(2), 695-710, doi:10.5194/bg-7-695-2010). The sample filters were unfolded and placed face up into acid-bathed and combusted scintillation vials. Along with each set of samples, 10 different volumes (ranging 0 - 0.5 mL) of 0.1 M KH2PO4 solution were added to scintillation vials. 2 mL of a 0.017 M MgSO4 drying solution was added to each scintillation vial and then all vials were placed into an 80-90 °C oven overnight to dry. After drying, the vials were heated at 500 °C for 2 hours, then left to cool before adding 5 mL of 0.2 M HCl to each vial and being returned to the 80-90&nbsp;°C&nbsp;oven for 30 minutes after being brought up to temperature. The solutions were then transferred to 15 mL glass centrifuge tubes (prepared in the same way as the scintillation vials). The sample vials were rinsed with 5 mL of milli-Q each, which was also added to the centrifuge tubes. 1 mL of a mixed reagent containing 2:5:1:2 parts Ammonium Molybdate ((NH4)6Mo7O24), 5.0 N H2SO4, Potassium Antimonyl Tartrate (C8H4K2O12Sb2), and Ascorbic Acid (C6H8O6) respectively was added to each centrifuge tube in 30-second intervals. Each of the sample tubes was centrifuged to isolate any glass fibers that could interfere with the absorbance reading. Lastly, after allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements.</p>
<p><strong>POC/PON assay:</strong><br />
POC and PON measurements were analyzed both in-lab at UCI. The preparation for all samples was the same; the samples were each removed from their foil packets and placed into acid-bathed and combusted scintillation vials and dried in a 55 °C oven overnight. The scintillation vials were then placed in a desiccator containing a beaker of 12 M HCl overnight before being dried at 55 °C at least overnight once more. Samples sent to UCSB were then capped and shipped to the lab. Samples analyzed at UCI were packed into tin packets alongside Atropine (C17H23NO3) standards (ranging from 0.2-1.5 mg) and measured using a Flash EA elemental analyzer.</p>
<p>Associated GO-SHIP I07N underway and bottle datasets can be found on the CLIVAR and Carbon Hydrographic Data Office Section I07N homepage:&nbsp;https://cchdo.ucsd.edu/cruise/33RO20180423.</p>
Specified by the Principal Investigator(s)
<p><strong>Researcher processing notes:</strong></p>
<ul>
<li>Matlab v2021a and R4.1.0 were used for data processing and wrangling.</li>
<li>Blank values in this dataset are displayed as "nd" for "no data."</li>
</ul>
<p><strong>BCO-DMO processing notes:</strong></p>
<ul>
<li>Rounded Latitude and Longitude values to 5th decimal place</li>
<li>In&nbsp;columns&nbsp;POC_µM,&nbsp;PON_µM,&nbsp;POP_µM,&nbsp;POC_large_µmol,&nbsp;PON_large_µmol, the character "µ" was changed to "u"</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
PI Supplied Instrument Name: CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts) PI Supplied Instrument Description:Samples analyzed at UCI were packed into tin packets alongside Atropine (C17H23NO3) standards (ranging from 0.2-1.5 mg) and measured using a Flash EA elemental analyzer. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
PI Supplied Instrument Name: Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts) PI Supplied Instrument Description:After allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: RB1803
RB1803
Community Standard Description
International Council for the Exploration of the Sea
NOAA Ship Ronald H. Brown
vessel
RB1803
Denis Volkov
National Oceanic and Atmospheric Administration
http://dx.doi.org/10.7942/C25H2B
Report describing RB1803
Community Standard Description
International Council for the Exploration of the Sea
NOAA Ship Ronald H. Brown
vessel