http://lod.bco-dmo.org/id/dataset/879380
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-08-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
CTD profiles collected with RV Connecticut on the Continental shelf and slope south of Montauk, NY on 14-15 June 2022.
2022-09-16
publication
2022-09-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-09-16
publication
https://doi.org/10.26008/1912/bco-dmo.879380.1
George McManus
University of Connecticut
principalInvestigator
Laura A. Katz
Smith College
principalInvestigator
Luciana Santoferrara
Hofstra University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: McManus, G., Santoferrara, L., Katz, L. A. (2022) CTD profiles collected with RV Connecticut on the Continental shelf and slope south of Montauk, NY on 14-15 June 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-09-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.879380.1 [access date]
CTD profiles RV Connecticut June 2022 Dataset Description: Methods and Sampling: <p>Seven stations on the shelf and slope south of Montauk, NY on 14-15 June 2022 were sampled.&nbsp; Basic hydrographic properties, including temperature, salinity, dissolved oxygen, and pH were measured and rosette samples were taken for chlorophyll, nutrients, and microplankton.&nbsp; Water was filtered for metagenomics and metatranscriptomics and picked individual microplankters for sequencing.&nbsp; Three to ten depths were sampled at each station.&nbsp; Our shallowest station was on the shelf at 38m and the deepest was at the slope edge at 2400m.&nbsp; Due to limitations of the CTD our deepest sampled depth was 1150m. At the two shallowest stations, we also deployed a Wetstar fluorometer for phytoplankton fluorescence.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924570 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924570
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924527 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924527
onGoing
George McManus
University of Connecticut
860-405-8602
Department of Marine Sciences, University of Connecticut 1080 Shennecossett Rd.
Groton
CT
06340
USA
george.mcmanus@uconn.edu
pointOfContact
Laura A. Katz
Smith College
413-585-3825
Department of Biological Sciences Smith College Burton Hall 201
Northampton
MA
01063
USA
lkatz@smith.edu
pointOfContact
Luciana Santoferrara
Hofstra University
516-463-7718
Biology Dept Room 301 Gittleson Hall
Hempstead
NY
11549-1000
USA
luciana.santoferrara@hofstra.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
latitude
longitude
Date
Time
density
depth
Fluorescence
oxygen
pH
salinity
scan
Temperature
flag
ISO_DateTime_Local
ISO_DateTime_UTC
Wetstar fluorometer
theme
None, User defined
station
latitude
longitude
date
time of day
density
depth
fluorescence
dissolved Oxygen
pH
salinity
event
water temperature at measurement depth
flag
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 9
WET Labs (Sea-Bird WETLabs) WETStar fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Combining single-cell and community 'omics' to test hypotheses about diversity and function of planktonic ciliates
http://microzooplankton.uconn.edu
Collaborative Research: Combining single-cell and community 'omics' to test hypotheses about diversity and function of planktonic ciliates
<p><em>NSF Award Abstract:</em><br />
Planktonic ciliates are key members of marine food webs where they serve diverse roles, including as food chain links between smaller microbes and larger plankton. Due to their small size and difficulties in identifying and cultivating them, we know less about ciliate diversity and distributions in the ocean than we do about larger organisms such as fish and invertebrates. Previous work from this team measured ciliate diversity in coastal waters and found that distinct genetic variants were separated in time and space in a way that could be related to factors such as ocean temperature, salinity, and depth gradients. Many questions remained unanswered, and it is important to understand the environmental factors that control the diversity and distribution of plankton such as ciliates to predict how these organisms may respond to a changing enviroment in the coming decades. This project focuses on: 1) how ciliate species are delineated using single-cell genomics and transcriptomics; 2) DNA-based studies of all ciliates and other planktonic members of the SAR clade (Stramenopila, Alveolata, Rhizaria), which will provide ecological context; 3) in situ gene expression by single-cell and meta- transcriptomics; and 4) laboratory studies of gene expression in cultivated ciliate species. This project involves training of postdoctoral scholars, graduate students, and undergraduates. The researchers are committed to creating diverse and inclusive research labs; recruitment of participants will be done through partnership with appropriate groups on our campuses. The project integrates with summer Research Experiences for Undergraduates (REU) activities at both Smith College and UCONN (including the UCONN/Mystic Aquarium joint REU), which are especially focused on underrepresented students. This project also enhances efforts to broaden understanding of biodiversity in partnership with the UCONN Noyce Scholars Program, which facilitates career-changing STEM professionals to become teachers in underserved secondary schools.</p>
<p>This project will assess distributions of reproductively-isolated species, determined using a new method to characterize regions of the ciliate germline genome. Furthermore, it will use phylogenomic methods to identify clade-specific transcripts (e.g. those of spirotrich ciliates) within metatranscriptomes from the shelf environment and to expand knowledge of ciliate function with single-cell transcriptomics of field-collected cells. These approaches will be a substantial improvement over the culture-based methods that are potentially biased towards "weedy" species in the ocean. The combination of definitive species identification with assessment of function via single-cell and meta- transcriptomics promises to provide significant advances in marine plankton ecology. The research focuses on two broad questions: 1) does the observed high diversity in phylogenetically-informative genes reflect reproductive isolation and functional differentiation in planktonic ciliates? and 2) do different co-occurring species of planktonic ciliates show substantial functional differences that correspond to different niches in the ocean? The project assesses species boundaries (i.e. reproductive isolation) through analyses of patterns in the germline micronuclei of planktonic ciliate morphospecies; characterizes transitions of closely-related ciliates across ecological gradients in the ocean; and examines functional differences within and between species, and in communities, through analyses of transcriptomics.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Ciliate Omics
largerWorkCitation
project
eng; USA
oceans
-71.7138
-71.3587
39.3804
40.994
2022-06-14
2022-06-15
New England continental shelf
0
BCO-DMO catalogue of parameters from CTD profiles collected with RV Connecticut on the Continental shelf and slope south of Montauk, NY on 14-15 June 2022.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/880567.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/880568.rdf
Name: latitude
Units: decimal degrees
Description: <p>Station latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/880569.rdf
Name: longitude
Units: decimal degrees
Description: <p>Station longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/880570.rdf
Name: Date
Units: unitless
Description: <p>Date of CTD sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/880571.rdf
Name: Time
Units: unitless
Description: <p>Start time of CTD sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/880572.rdf
Name: density
Units: kg/m^3
Description: <p>Density</p>
http://lod.bco-dmo.org/id/dataset-parameter/880573.rdf
Name: depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/880574.rdf
Name: Fluorescence
Units: mg/m^3
Description: <p>Fluorescence, arbitrary</p>
http://lod.bco-dmo.org/id/dataset-parameter/880575.rdf
Name: oxygen
Units: mg/L
Description: <p>dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/880576.rdf
Name: pH
Units: unitless
Description: <p>pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/880577.rdf
Name: salinity
Units: unitless
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/880578.rdf
Name: scan
Units: unitless
Description: <p>sampling event number for station</p>
http://lod.bco-dmo.org/id/dataset-parameter/880579.rdf
Name: Temperature
Units: Degrees Celsius (°C)
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/880580.rdf
Name: flag
Units: untless
Description: <p>Quality flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/880581.rdf
Name: ISO_DateTime_Local
Units: units
Description: <p>Sampling date and time (Eastern Daylight Time (GMT-4)) in ISO 8601 format yyyy-mm-ddTHH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/880582.rdf
Name: ISO_DateTime_UTC
Units: units
Description: <p>Sampling date and time (UTC) in ISO 8601 format yyyy-mm-ddTHH:MM:SSZ</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3082456
https://darchive.mblwhoilibrary.org/bitstream/1912/29356/1/CTD_files.zip
download
40201605
https://darchive.mblwhoilibrary.org/bitstream/1912/29356/2/dataset-879380_ctd-profiles-rv-connecticut-june-2022__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.879380.1
download
onLine
dataset
<p>Seven stations on the shelf and slope south of Montauk, NY on 14-15 June 2022 were sampled.&nbsp; Basic hydrographic properties, including temperature, salinity, dissolved oxygen, and pH were measured and rosette samples were taken for chlorophyll, nutrients, and microplankton.&nbsp; Water was filtered for metagenomics and metatranscriptomics and picked individual microplankters for sequencing.&nbsp; Three to ten depths were sampled at each station.&nbsp; Our shallowest station was on the shelf at 38m and the deepest was at the slope edge at 2400m.&nbsp; Due to limitations of the CTD our deepest sampled depth was 1150m. At the two shallowest stations, we also deployed a Wetstar fluorometer for phytoplankton fluorescence.</p>
Specified by the Principal Investigator(s)
<p>The CTD data were processed with SeaBird's Seasoft program and converted to spreadsheet format.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: CTD Sea-Bird 9 Instrument Short Name:CTD SBE 9 Instrument Description: The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Wetstar fluorometer
Wetstar fluorometer
PI Supplied Instrument Name: Wetstar fluorometer PI Supplied Instrument Description:Wetstar fluorometer for phytoplankton fluorescence Instrument Name: WET Labs (Sea-Bird WETLabs) WETStar fluorometer Instrument Short Name:WETStar Instrument Description: Submersible fluorometer designed for through-flow or pumped CTD applications manufactured by WetLabs and which can be configured for various types of fluorescence. The probe has a temperature range of 0-30 degrees C and a depth rating of 600 meters. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0075/