<div><p>Samples were collected using standard oceanographic techniques. A CTD Rosette with 24 10L Niskin bottles was lowered to the maximum sampling depth and then brought back to the surface. Seawater was sampled directly from Niskin® bottles into acid-washed 1.2 l polycarbonate bottles through 210 µm Nitex® mesh (Wildco, Yulee, FL) to remove large grazers.</p>
<p>Methodology described in depth in Turk-Kubo et al. (2021)</p></div>
Single cell N2 fixation measurements
<div><p>These data were published in Turk-Kubo et al. (2021). Table 1, Figure 6, Table S6, Table S7.</p></div>
SCCS Single Cell N2 Fixation Measurements
<div><p>Subsamples (95 ml) taken from the incubation bottles were fixed with sterile-filtered formaldehyde (MilliporeSigma) at a final concentration of 1.85% (v/v) for >1 h at 4 °C, then concentrated with 0.6 µm pore-size polycarbonate filters (MilliporeSigma) under gentle vacuum, air-dried and stored at −80 °C. UCYN-A1 and UCYN-A2 symbioses were targeted using 5′-horseradish peroxidase-labeled oligonucleotide probes (Biomers, Inc., Ulm/Donau, Germany), using helper and competitor probes for both symbionts and hosts (Biomers). Protocols for CARD-FISH hybridizations followed procedures described in detail by Cabello et al. 2016.</p>
<p>Samples were visualized, transferred, and mapped to facilitate nanoSIMS analyses according to protocols detailed in Mills et al. 2020. Individual symbioses were analyzed on a Cameca nanoSIMS 50 L at the Stanford Nano Shared Facilities (Stanford, CA). Once targets were located using the charged-coupled device camera and the secondary electron image, image fields were rastered with a 16 keV Cesium primary ion beam (~5 pA) focused into ca. 120 nm spot diameter (256 × 256 pixels, dwell time 1 ms per pixel). Images of 12C−, 13C−, 12C14N−and 12C15N− were measured over 30–100 planes with a mass resolving power of ca. 8000. Regions of interest were defined around UCYN-A and host cells using <a href="mailto:Look@nanoSIMS">Look@nanoSIMS</a>. Isotope ratios of UCYN-A and haptophyte cells were calculated as described in Mills et al. 2020.</p>
<p>Methodology described in depth in Turk-Kubo et al. (2021)</p></div>
881078
SCCS Single Cell N2 Fixation Measurements
2022-09-26T15:38:19-04:00
2022-09-26T15:38:19-04:00
2023-12-01T11:03:04-05:00
urn:bcodmo:dataset:881078
Single cell N2 fixation measurements from UCYN-A symbiosis in the Southern California Current System from May 2017 (SP1714) and October (SP1724) SCCS cruises.
Single cell N2 fixation measurements from UCYN-A symbiosis in the Southern California Current System from May 2017 (SP1714) and October (SP1724) SCCS cruises.
false
Zehr, J. P., Arrigo, K. R., Turk-Kubo, K. (2023) Single cell N2 fixation measurements from UCYN-A symbiosis in the Southern California Current System from May 2017 (SP1714) and October (SP1724) SCCS cruises. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-30 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/881078 [access date]
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1
false
2023-11-30
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