http://lod.bco-dmo.org/id/dataset/883037
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-10-27
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Temperature from seagrass wasting disease mesocosm experiments at Bodega Marine Laboratory in June-July 2015
2022-10-27
publication
2022-10-27
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-11-22
publication
https://doi.org/10.26008/1912/bco-dmo.883037.1
A. Randall Hughes
Northeastern University
principalInvestigator
Katherine DuBois
University of California-Davis
principalInvestigator
Melissa Kardish
University of California-Davis
principalInvestigator
Forest Schenck
Northeastern University
principalInvestigator
John J. Stachowicz
University of California-Davis
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Schenck, F., DuBois, K., Kardish, M., Stachowicz, J. J., Hughes, A. R. (2022) Temperature from seagrass wasting disease mesocosm experiments at Bodega Marine Laboratory in June-July 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.883037.1 [access date]
Methods and Sampling: <p><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">We used a substitutive design to test the effects of eelgrass (</span><span style="font-size:13px">Zostera marina)&nbsp;</span><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">genotypic identity (eight genotypes), diversity (monocultures of 1 genotype vs. polycultures of 4 genotypes), and temperature (ambient or + 3.2° C) on the prevalence and intensity of&nbsp;</span><em>Labyrinthula</em><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">&nbsp;over eight weeks in an array of flow-through 120-L mesocosms at the Bodega Marine Laboratory in Bodega Bay, CA. We assigned ten pots - two unique polyculture combinations and each of the eight monocultures - to each of ten mesocosms, with five mesocosms per temperature treatment (see DuBois et al. 2020 for a diagram of the experimental set up). All mesocosms received sand-filtered flow-through seawater at a rate of approximately 0.8-1.0 L min</span><sup>-1</sup><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">. We allowed the plants to acclimate for one month prior to initiating the temperature treatments. We maintained an ambient temperature treatment by cooling flow-through seawater in a head tank by approximately 1˚C using an Aqua Logic Delta Star in-line titanium chiller. Seawater in the elevated temperature treatment was raised approximately 3˚C above the ambient treatment in a separate header tank using Process Technologies titanium immersion heaters. This level of warming mimicked the 2014-15 extreme warming events in the Northern Pacific called “The Blob”, which raised summer ocean temperatures three standard deviations above the long-term average (Sanford et al. 2019).<br />
<br />
<strong>Life Sciences Identifiers (LSID) for taxonomic names:</strong></span><br />
Zostera marina (urn:lsid:marinespecies.org:taxname:145795)<br />
Labyrinthula zosterae (urn:lsid:marinespecies.org:taxname:395093)<br />
Labyrinthula (urn:lsid:marinespecies.org:taxname:119090)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1652320 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1652320
completed
A. Randall Hughes
Northeastern University
781-581-7370
430 Nahant Rd.
Nahant
MA
01908
USA
ann.hughes@northeastern.edu
pointOfContact
Katherine DuBois
University of California-Davis
Bowdoin College Schiller Coastal Studies Center 6775 College Station
Brunswick
ME
04011-8479
USA
khoering@gmail.com
pointOfContact
Melissa Kardish
University of California-Davis
707-875-1968
University of California Davis Bodega Marine Lab P.O.Box 247, 2099 Westshore Road
Bodega Bay
CA
94923
USA
mrkardish@ucdavis.edu
pointOfContact
Forest Schenck
Northeastern University
30 Emerson Avenue
Gloucester
MA
01930
USA
forest.schenck@mass.gov
pointOfContact
John J. Stachowicz
University of California-Davis
530-752-1113
Department of Evolution and Ecology 1 Shields Ave
Davis
CA
95616
USA
jjstachowicz@ucdavis.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
temperature_treatment
bin
date_time
temperature
ISO_DateTime_UTC
flow through tanks
Aqua Logic Delta Star in-line titanium chiller
Process Technologies titanium immersion heater
Onset Hobo Pendant Temperature Data Loggers
theme
None, User defined
treatment
sample description
Date and time
water temperature at measurement depth
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Aquarium
Aquarium chiller
Immersion heater
Onset HOBO Pendant Temperature/Light Data Logger
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Linking genetic diversity, population density, and disease prevalence in seagrass and oyster ecosystems
https://osprey.bco-dmo.org/project/709942
CAREER: Linking genetic diversity, population density, and disease prevalence in seagrass and oyster ecosystems
<p><em>NSF Award Abstract:</em><br />
Disease outbreaks in the ocean are increasing, causing losses of ecologically important marine species, but the factors contributing to these outbreaks are not well understood. This 5-year CAREER project will study disease prevalence and intensity in two marine foundation species - the seagrass Zostera marina and the Eastern oyster Crassostrea virginica. More specifically, host-disease relationships will be explored to understand how genetic diversity and population density of the host species impacts disease transmission and risk. This work will pair large-scale experimental restorations and smaller-scale field experiments to examine disease-host relationships across multiple spatial scales. Comparisons of patterns and mechanisms across the two coastal systems will provide an important first step towards identifying generalities in the diversity-density-disease relationship. To enhance the broader impacts and utility of this work, the experiments will be conducted in collaboration with restoration practitioners and guided by knowledge ascertained from key stakeholder groups. The project will support the development of an early career female researcher and multiple graduate and undergraduate students. Students will be trained in state-of-the-art molecular techniques to quantify oyster and seagrass parasites. Key findings from the surveys and experimental work will be incorporated into undergraduate courses focused on Conservation Biology, Marine Biology, and Disease Ecology. Finally, students in these courses will help develop social-ecological surveys and mutual learning games to stimulate knowledge transfer with stakeholders through a series of workshops.</p>
<p>The relationship between host genetic diversity and disease dynamics is complex. In some cases, known as a dilution effect, diversity reduces disease transmission and risk. However, the opposite relationship, known as the amplification effect, can also occur when diversity increases the risk of infection. Even if diversity directly reduces disease risk, simultaneous positive effects of diversity on host density could lead to amplification by increasing disease transmission between infected and uninfected individuals. Large-scale field restorations of seagrasses (Zostera marina) and oysters (Crassostrea virginica) will be utilized to test the effects of host genetic diversity on host population density and disease prevalence/intensity. Additional field experiments independently manipulating host genetic diversity and density will examine the mechanisms leading to dilution or amplification. Conducting similar manipulations in two marine foundation species - one a clonal plant and the other a non-clonal animal - will help identify commonalities in the diversity-density-disease relationship. Further, collaborations among project scientists, students, and stakeholders will enhance interdisciplinary training and help facilitate the exchange of information to improve management and restoration efforts. As part of these efforts, targeted surveys will be used to document the perceptions and attitudes of managers and restoration practitioners regarding genetic diversity and its role in ecological resilience and restoration.</p>
Seagrass and Oyster Ecosystems
largerWorkCitation
project
eng; USA
oceans
-123.06572
-123.06572
38.31753
38.31753
2015-07-01
2015-09-14
Coastal New England
0
BCO-DMO catalogue of parameters from Temperature from seagrass wasting disease mesocosm experiments at Bodega Marine Laboratory in June-July 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/883483.rdf
Name: temperature_treatment
Units: unitless
Description: <p>Descriptor of the temperature treatment applied to seawater: ambient (cooled flow-through seawater by approximately 1oC); elevated (heated flow-through seawater by approximately 3oC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/883484.rdf
Name: bin
Units: unitless
Description: <p>Unique identifier number assigned to each of the ten 120 L mesocosms at the Bodega Marine Laboratory supplied with flow-through seawater</p>
http://lod.bco-dmo.org/id/dataset-parameter/883485.rdf
Name: date_time
Units: unitless
Description: <p>Date and time of temperature measurement (Time zone: PST/PDT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/883486.rdf
Name: temperature
Units: Degrees Celsius
Description: <p>Seawater temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/884151.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of temperature measurement (Time zone: UTC) in ISO8601 format.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6731125
https://darchive.mblwhoilibrary.org/bitstream/1912/29521/1/dataset-883037_mesocosm-warming-experiment-temperature__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.883037.1
download
onLine
dataset
<p><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">We used a substitutive design to test the effects of eelgrass (</span><span style="font-size:13px">Zostera marina)&nbsp;</span><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">genotypic identity (eight genotypes), diversity (monocultures of 1 genotype vs. polycultures of 4 genotypes), and temperature (ambient or + 3.2° C) on the prevalence and intensity of&nbsp;</span><em>Labyrinthula</em><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">&nbsp;over eight weeks in an array of flow-through 120-L mesocosms at the Bodega Marine Laboratory in Bodega Bay, CA. We assigned ten pots - two unique polyculture combinations and each of the eight monocultures - to each of ten mesocosms, with five mesocosms per temperature treatment (see DuBois et al. 2020 for a diagram of the experimental set up). All mesocosms received sand-filtered flow-through seawater at a rate of approximately 0.8-1.0 L min</span><sup>-1</sup><span style="color:rgb(0, 0, 0); font-family:times new roman,serif; font-size:16px">. We allowed the plants to acclimate for one month prior to initiating the temperature treatments. We maintained an ambient temperature treatment by cooling flow-through seawater in a head tank by approximately 1˚C using an Aqua Logic Delta Star in-line titanium chiller. Seawater in the elevated temperature treatment was raised approximately 3˚C above the ambient treatment in a separate header tank using Process Technologies titanium immersion heaters. This level of warming mimicked the 2014-15 extreme warming events in the Northern Pacific called “The Blob”, which raised summer ocean temperatures three standard deviations above the long-term average (Sanford et al. 2019).<br />
<br />
<strong>Life Sciences Identifiers (LSID) for taxonomic names:</strong></span><br />
Zostera marina (urn:lsid:marinespecies.org:taxname:145795)<br />
Labyrinthula zosterae (urn:lsid:marinespecies.org:taxname:395093)<br />
Labyrinthula (urn:lsid:marinespecies.org:taxname:119090)</p>
Specified by the Principal Investigator(s)
<p>Code that includes temperature analysis associated with this experiment: All code was written and run in R (version 3.6.1, www.R-project.org). Github repository link: https://github.com/schenckf/BWE_Experiment and Zenodo DOI: https://doi.org/10.5281/zenodo.7129500. A general description of the code is included in the repository release.<br />
<br />
<span style="font-size:13px">BCO-DMO Processing:</span></p>
<div><span style="font-size:13px">* Imported data from source file "mesocosm_warming_temperature_data.csv" into the BCO-DMO data system.<br />
Data file imported using missing data identifier "NA".</span><br />
* Modified parameter (column) names to conform with BCO-DMO naming conventions.<br />
* ISO_DateTime_UTC column added from date_time (local timezone). converted to ISO 8601 format</div>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
flow through tanks
flow through tanks
PI Supplied Instrument Name: flow through tanks Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
Aqua Logic Delta Star in-line titanium chiller
Aqua Logic Delta Star in-line titanium chiller
PI Supplied Instrument Name: Aqua Logic Delta Star in-line titanium chiller PI Supplied Instrument Description:We maintained an ambient temperature treatment by cooling flow-through seawater in a head tank by approximately 1˚C using an Aqua Logic Delta Star in-line titanium chiller. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
Process Technologies titanium immersion heater
Process Technologies titanium immersion heater
PI Supplied Instrument Name: Process Technologies titanium immersion heater PI Supplied Instrument Description:Seawater in the elevated temperature treatment was raised approximately 3˚C above the ambient treatment in a separate header tank using Process Technologies titanium immersion heaters Instrument Name: Immersion heater Instrument Short Name:Immersion heater Instrument Description: Submersible heating element for water tanks and aquaria.
Onset Hobo Pendant Temperature Data Loggers
Onset Hobo Pendant Temperature Data Loggers
PI Supplied Instrument Name: Onset Hobo Pendant Temperature Data Loggers Instrument Name: Onset HOBO Pendant Temperature/Light Data Logger Instrument Short Name:Onset HOBO Pendant Temp/Light Instrument Description: The Onset HOBO (model numbers UA-002-64 or UA-001-64) is an in-situ instrument for wet or underwater applications. It supports light intensity, soil temperature, temperature, and water temperature. A two-channel logger with 10-bit resolution can record up to approximately 28,000 combined temperature and light measurements with 64K bytes memory. It has a polypropylene housing case. Uses an optical USB to transmit data. A solar radiation shield is used for measurement in sunlight. Temperature measurement range: -20 deg C to 70 deg C (temperature). Light measurement range: 0 to 320,000 lux. Temperature accuracy: +/- 0.53 deg C from 0 deg C to 50 deg C. Light accuracy: Designed for measurement of relative light levels. Water depth rating: 30 m.