http://lod.bco-dmo.org/id/dataset/884220 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2022-11-23 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Physiology color score extracted from pictures taken during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. 2023-01-31 publication 2023-01-31 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-02-07 publication https://doi.org/10.26008/1912/bco-dmo.884220.1 Hollie Putnam University of Rhode Island principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Strand, E., Putnam, H. (2023) Physiology color score extracted from pictures taken during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.884220.1 [access date] Thermal Stress Experiment: Color Score Physiology Dataset Description: Methods and Sampling: &lt;p&gt;From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species M. capitata and P. acuta were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. (21N 157 W ; depth 1m).&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756623 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756623 completed Hollie Putnam University of Rhode Island 808-554-1462 120 Flagg Rd. Kingston RI 02881 USA hputnam@uri.edu pointOfContact asNeeded Dataset Version: 1 Unknown Date Timepoint PLUG_ID Genotype Species Photo_Number Tank Treatment Red_Standard Green_Standard Blue_Standard Red_Coral Green_Coral Blue_Coral Notes Digital Camera Ruler with three red, blue, and green colored bands theme None, User defined date sample time sample identification species scientific name (binomial) file_name tank treatment No BCO-DMO term comments featureType BCO-DMO Standard Parameters Camera ruler instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration https://sites.rutgers.edu/coralbase/ NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration <p>NSF Abstract:</p> <p>The remarkable success of coral reefs is explained by interactions of the coral animal with its symbiotic microbiome that is comprised of photosynthetic algae and bacteria. This total organism, or "holobiont", enables high ecosystem biodiversity and productivity in coral reefs. These ecosystems are, however, under threat from a rapidly changing environment. This project aims to integrate information from the cellular to organismal level to identify key mechanisms of adaptation and acclimatization to environmental stress. Specific areas to be investigated include the role of symbionts and of epigenetics (molecular "marks" on coral DNA that regulate gene expression). These aspects will be studied in Hawaiian corals to determine whether they explain why some individuals are sensitive or resistant to environmental perturbation. Results from the proposed project will also provide significant genomic resources that will contribute to fundamental understanding of how complex biological systems generate emergent (i.e., unexpected) properties when faced with fluctuating environments. Broader impacts will extend beyond scientific advancements to include postdoctoral and student training in Science, Technology, Engineering and Mathematics (STEM). Data generated in the project will be used to train university students and do public outreach through live videos of experimental work, and short stop-action animations for topics such as symbiosis, genomics, epigenetics, inheritance, and adaptation. The research approaches and results will be shared with the public in Hawaii through the Hawaii Institute of Marine Biology education department and presentations at Hawaiian hotels, as well as at Rutgers University through its 4-H Rutgerscience Saturdays and 4-H Rutgers Summer Science Programs.</p> <p>Symbiosis is a complex and ecologically integrated interaction between organisms that provides emergent properties key to their survival. Such is the case for the relationship between reef-building corals and their microbiome, a meta-organism, where nutritional and biogeochemical recycling provide the necessary benefits that fuel high reef productivity and calcification. The rapid warming and acidification of our oceans threatens this symbiosis. This project addresses how relatively stress resistant and stress sensitive corals react to the environmental perturbations of increased temperature and reduced pH. It utilizes transcriptomic, epigenetic, and microbial profiling approaches, to elucidate how corals respond to environmental challenges. In addition to this profiling, work by the BSF Israeli partner will implement powerful analytical techniques such as network theory to detect key transcriptional hubs in meta-organisms and quantify biological integration. This work will generate a stress gene inventory for two ecologically important coral species and a (epi)genome and microbiome level of understanding of how they respond to the physical environment. Acknowledgment of a role for epigenetic mechanisms in corals overturns the paradigm of hardwired genetic control and highlights the interplay of genetic and epigenetic variation that may result in emergent evolutionary and ecologically relevant properties with implications for the future of reefs. Furthermore, clarifying the joint contribution of the microbiome and host in response to abiotic change will provide an important model in metazoan host-microbiome biotic interactions.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Holobiont Integration largerWorkCitation project eng; USA oceans 2023-01-31 Hawaii, Rhode Island, New Jersey, Israel 0 BCO-DMO catalogue of parameters from Physiology color score extracted from pictures taken during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/886602.rdf Name: Date Units: unitless Description: <p>Date of measurement (Month-Day-Year)</p> http://lod.bco-dmo.org/id/dataset-parameter/886603.rdf Name: Timepoint Units: unitless Description: <p>Categorical timepoint of experiment</p> http://lod.bco-dmo.org/id/dataset-parameter/886604.rdf Name: PLUG_ID Units: unitless Description: <p>Plug ID indicating coral ID</p> http://lod.bco-dmo.org/id/dataset-parameter/886605.rdf Name: Genotype Units: unitless Description: <p>Genotype ID</p> http://lod.bco-dmo.org/id/dataset-parameter/886606.rdf Name: Species Units: unitless Description: <p>Coral species </p> http://lod.bco-dmo.org/id/dataset-parameter/886607.rdf Name: Photo_Number Units: unitless Description: <p>ID of photograph taken</p> http://lod.bco-dmo.org/id/dataset-parameter/886608.rdf Name: Tank Units: unitless Description: <p>Tank ID</p> http://lod.bco-dmo.org/id/dataset-parameter/886609.rdf Name: Treatment Units: unitless Description: <p>Temperature treatment</p> http://lod.bco-dmo.org/id/dataset-parameter/886610.rdf Name: Red_Standard Units: pixels Description: <p>Color value of red standard, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886611.rdf Name: Green_Standard Units: pixels Description: <p>Color value of green standard, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886612.rdf Name: Blue_Standard Units: pixels Description: <p>Color value of blue standard, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886613.rdf Name: Red_Coral Units: pixels Description: <p>Red color in coral measurement, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886614.rdf Name: Green_Coral Units: pixels Description: <p>Green color in coral measurement, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886615.rdf Name: Blue_Coral Units: pixels Description: <p>Blue color in coral measurement, raw RGB values extracted from ImageJ. </p> http://lod.bco-dmo.org/id/dataset-parameter/886616.rdf Name: Notes Units: units Description: <p>Notes</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 182887 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/309eb222-9952-50ea-9718-2d2fe5742aa3/content download https://doi.org/10.26008/1912/bco-dmo.884220.1 download onLine dataset &lt;p&gt;From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species M. capitata and P. acuta were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. (21N 157 W ; depth 1m).&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Each sample was photographed using a digital camera with a red/blue/green color standard.&amp;nbsp;Red/blue/green values that were extracted in ImageJ,&amp;nbsp;version 1.51 (Schneider et al. 2012) from the coral were standardized to the color standards by dividing the experimental value observed in the coral against the corresponding actual recorded value from the color standards (Edmunds et al. 2003). Using the normalized intensity values from each color channel, a bleaching score was quantified as PC1 from principle components analysis of these data. As stress is prolonged and bleaching becomes more pronounced, the red/blue/green color readings from the coral will equalize around the same number because white is an equal expression of all colors. All nubbins available at each time points were used for color assessment.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Digital Camera Digital Camera PI Supplied Instrument Name: Digital Camera Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/ Ruler with three red, blue, and green colored bands Ruler with three red, blue, and green colored bands PI Supplied Instrument Name: Ruler with three red, blue, and green colored bands Instrument Name: ruler Instrument Short Name: Instrument Description: A device used for measuring or for drawing straight lines, consisting of an elongated piece of rigid or semi-rigid material marked with units for measurement.  Device that allows one or more physical dimensions of a sample or specimen to be determined by visible comparison against marked graduations in units of measurement of dimension length.