http://lod.bco-dmo.org/id/dataset/885679
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-12-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Symbiont psbA and ORF host sequences generated as part of a study of pCO2 variability on the reef-building coral Pocillopora damicornis conducted at Heron Island Research Station, Heron Island, southern Great Barrier Reef in 2021
2022-12-20
publication
2022-12-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-01-18
publication
https://doi.org/10.26008/1912/bco-dmo.885679.1
Katie Barott
University of Pennsylvania
principalInvestigator
Kristen Brown
University of Pennsylvania
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Barott, K., Brown, K. T. (2023) Symbiont psbA and ORF host sequences generated as part of a study of pCO2 variability on the reef-building coral Pocillopora damicornis conducted at Heron Island Research Station, Heron Island, southern Great Barrier Reef in 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-12-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.885679.1 [access date]
Dataset Description: <p>The truncates sequences were submitted to GenBank under the following accession numbers (accessible from the below "popsets"):</p>
<p>"Cladocopium latusorum photosystem II D1/D2 subunit (psbA) gene, partial cds; chloroplast."<br />
* popset: https://www.ncbi.nlm.nih.gov/popset/2310683771<br />
* accessions OP279755 – OP279774&nbsp;</p>
<p>"Pocillopora damicornis ATP synthase subunit 6 (ATP6) gene, complete cds; and control region, complete sequence; mitochondrial."<br />
* popset: https://www.ncbi.nlm.nih.gov/popset/2310683883<br />
* accessions OP296521 - OP296503<br />
<br />
This dataset contains two fasta files aggregating sequences from the above popsets at NCBI.</p> Methods and Sampling: <p><strong>Genetic analyses</strong><br />
<br />
A small chip (2‒3mm) of each fragment (preserved in 100% ethanol and stored at -80°C) was used to extract gDNA, cf. methods described in (LaJeunesse et al., 2003) and the DNA eluted in ddH2O. The coral species field-based identification using morphological characteristics (sensu Pocillopora damicornis) was confirmed with a genetic assay (Schmidt-Roach et al., 2013) using the mitochondrial ORF region. The DNA was diluted 1:20 and PCR was done using the FATP/RORF primers cf. (Flot and Tillier, 2007) and NEB TAQ Polymerase (M0320) following the manufacturers routine PCR protocol under cycling conditions: 95°C 2 min initial denaturation, 35 cycles of 94°C 30s, 53°C 30s, 68°C 1 min, and a final extension of 68°C for 10 min. All but two specimens (one from each habitat) were successfully amplified; these were cleaned using ExoSap-IT and Sanger sequenced in a single direction using the forward FATP primer at the Australian Genome Research Facility (Brisbane, Australia). In addition, the species of resident coral endosymbionts (Symbiodiniaceae) were identified using PCR amplification of the ITS2 rDNA region followed by denaturing gradient electrophoresis alongside local ‘marker’ specimens from Pocillopora species with previously confirmed symbiont identifications (cf. Sampayo et al., 2009). To cross-confirm, dominant bands of characteristic profiles were excised, re-amplified and sequenced using the forward ITS2intfor and reverse ITS2reverse primers (cf. LaJeunesse et al., 2003;&nbsp;Sampayo et al., 2009). In addition, the chloroplast minicircle psbA non-coding and partial coding region were amplified using two different primer sets and conditions. Initially, the 7.4-Forw/7.8-Rev primers were used cf. (Moore et al. 2003) but some samples failed to amplify. Some of these yielded successful PCR amplicons with the psbAFor-1/psbARev-1 primers (LaJeunesse and Thornhill, 2011).&nbsp;Successful reactions were cleaned using ExoSap-IT and bi-directionally Sanger sequenced with their respective primers at the Australian Genome Research Facility. Sequence chromatograms for all regions (host mtORF; symbiont ITS2 rDNA and chloroplast psbA) were visually inspected and compared to holotype sequences from described Pocillopora species (Schmidt-Roach et al., 2013) and recently described symbiont species derived from these coral species (Turnham et al., 2021).&nbsp;The mtORF sequences from the study specimens all had 100% match with the P. damicornis (GenBank Accession numbers OP296503 – OP296521; 100% match to Pocillopora type alpha cf. (Schmidt-Roach et al., 2013), GenBank accession numbers JX985598 and JX985606).&nbsp;The ITS2 rDNA profiles as well as sequences matched previously described Cladocopium type ‘C1b-c, 42a’ from Heron Reef (Sampayo et al. 2007). For the psbA, 4 out of 24 samples returned poor sequence reads (2 from each environment). The remaining 20 psbA sequences were aligned with inclusion of reference sequences for recently described Cladocopium latusorum and C. pacificum (GenBank Accession numbers MW819755 - MW819779 and MW861711 - MW861727, respectively) as well as the sequence for C. goreaii (Turnham et al., 2021). Sequences were aligned using Geneious (MAFFT), checked and adjusted by eye, which was particularly important given the large number of indels present in the psbA sequence dataset. All sequences were truncated to the 7.4-Forw and 7.8-Rev primer binding sites, given that no differences were seen in the sequence regions extending outward to the psbA coding region psbAFor1/psbARev1 binding sites. The truncates sequences were submitted to GenBank under accession numbers OP279755 – OP279774 (https://www.ncbi.nlm.nih.gov/popset/2310683771).&nbsp; &nbsp;All indels were adjusted to represent a single base change, using gaps as a 5th character state, in subsequent phylogenetic analyses. A psbA (partial coding, and entire non-coding region) maximum parsimony majority-rule phylogeny was constructed using a heuristic search with bootstrap values calculated (TBR branch swapping, random addition, 1000 replicates) in PAUP vs. 4.0a (build 169; Turnham et al., 2021).</p>
<p>For more detailed information, please see: Brown et al. (2022).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1923743 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1923743
completed
Katie Barott
University of Pennsylvania
215-746-6911
433 South University Avenue Leidy Laboratories 326
Philadelphia
PA
19104
USA
kbarott@sas.upenn.edu
pointOfContact
Kristen Brown
University of Pennsylvania
433 S University Ave
Philadelphia
PA
19104-4544
USA
ktbrown@sas.upenn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress
https://osprey.bco-dmo.org/project/843347
Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress
<p><strong>NSF Award Abstract:</strong><br />
Coral reefs are incredibly diverse ecosystems that provide food, tourism revenue, and shoreline protection for coastal communities. The ability of coral reefs to continue providing these services to society is currently threatened by climate change, which has led to increasing ocean temperatures and acidity that can lead to the death of corals, the animals that build the reef framework upon which so many species depend. This project examines how temperature and acidification stress work together to influence the future health and survival of corals. The scientists are carrying out the project in Hawaii where they have found individual corals with different sensitivities to temperature stress that are living on reefs with different environmental pH conditions. This project improves understanding of how an individual coral's history influences its response to multiple stressors and helps identify the conditions that are most likely to support resilient coral communities. The project will generate extensive biological and physicochemical data that will be made freely available. Furthermore, this project supports the education and training of undergraduate and high school students and one postdoctoral researcher in marine science and coral reef ecology. Hands-on activities for high school students are being developed into a free online educational resource.</p>
<p>This project compares coral responses to acidification stress in populations experiencing distinct pH dynamics (high diel variability vs. low diel variability) and with distinct thermal tolerances (historically bleaching sensitive vs. tolerant) to learn about how coral responses to these two factors differ between coral species and within populations. Experiments focus on the two dominant reef builders found at these stable and variable pH reefs: Montipora capitata and Porites compressa. Individuals of each species exhibiting different thermal sensitivities (i.e., bleached vs. pigmented) were tagged during the 2015 global coral bleaching event. This system tests the hypotheses that 1) corals living on reefs with larger diel pH fluctuations have greater resilience to acidification stress, 2) coral resilience to acidification is a plastic trait that can be promoted via acclimatization, and 3) thermally sensitive corals have reduced capacity to cope with pH stress, which is exacerbated at elevated temperatures. Coral cells isolated from colonies from each environmental and bleaching history are exposed to acute pH stress and examined for their ability to recover intracellular pH in vivo using confocal microscopy, and the expression level of proteins predicted to be involved in this recovery (e.g., proton transporters) is examined via Western blot and immunolocalization. Corals from each pH history are exposed to stable and variable seawater pH in a controlled aquarium setting to determine the level of plasticity of acidification resilience and to test for pH acclimatization in this system. Finally, corals with different levels of thermal sensitivity are exposed to thermal stress and recovery, and their ability to regulate pH is examined over time. The results of these experiments help identify reef conditions that promote coral resilience to ocean acidification against the background of increasingly common thermal stress events, while advancing mechanistic understanding of coral physiology and symbiosis.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Coral Resilience
largerWorkCitation
project
eng; USA
oceans
151.55
151.55
-23.27
-23.27
2021-01-06
2021-04-06
Kaneohe Bay, Oahu, HI; Heron Island, Queensland, Australia
0
BCO-DMO catalogue of parameters from Symbiont psbA and ORF host sequences generated as part of a study of pCO2 variability on the reef-building coral Pocillopora damicornis conducted at Heron Island Research Station, Heron Island, southern Great Barrier Reef in 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
22074
https://darchive.mblwhoilibrary.org/bitstream/1912/29615/1/KB_psbaseqs_Genbank.fasta
download
21718
https://darchive.mblwhoilibrary.org/bitstream/1912/29615/2/KB_ORFseqs_GenBank.fasta
download
https://doi.org/10.26008/1912/bco-dmo.885679.1
download
onLine
dataset
<p><strong>Genetic analyses</strong><br />
<br />
A small chip (2‒3mm) of each fragment (preserved in 100% ethanol and stored at -80°C) was used to extract gDNA, cf. methods described in (LaJeunesse et al., 2003) and the DNA eluted in ddH2O. The coral species field-based identification using morphological characteristics (sensu Pocillopora damicornis) was confirmed with a genetic assay (Schmidt-Roach et al., 2013) using the mitochondrial ORF region. The DNA was diluted 1:20 and PCR was done using the FATP/RORF primers cf. (Flot and Tillier, 2007) and NEB TAQ Polymerase (M0320) following the manufacturers routine PCR protocol under cycling conditions: 95°C 2 min initial denaturation, 35 cycles of 94°C 30s, 53°C 30s, 68°C 1 min, and a final extension of 68°C for 10 min. All but two specimens (one from each habitat) were successfully amplified; these were cleaned using ExoSap-IT and Sanger sequenced in a single direction using the forward FATP primer at the Australian Genome Research Facility (Brisbane, Australia). In addition, the species of resident coral endosymbionts (Symbiodiniaceae) were identified using PCR amplification of the ITS2 rDNA region followed by denaturing gradient electrophoresis alongside local ‘marker’ specimens from Pocillopora species with previously confirmed symbiont identifications (cf. Sampayo et al., 2009). To cross-confirm, dominant bands of characteristic profiles were excised, re-amplified and sequenced using the forward ITS2intfor and reverse ITS2reverse primers (cf. LaJeunesse et al., 2003;&nbsp;Sampayo et al., 2009). In addition, the chloroplast minicircle psbA non-coding and partial coding region were amplified using two different primer sets and conditions. Initially, the 7.4-Forw/7.8-Rev primers were used cf. (Moore et al. 2003) but some samples failed to amplify. Some of these yielded successful PCR amplicons with the psbAFor-1/psbARev-1 primers (LaJeunesse and Thornhill, 2011).&nbsp;Successful reactions were cleaned using ExoSap-IT and bi-directionally Sanger sequenced with their respective primers at the Australian Genome Research Facility. Sequence chromatograms for all regions (host mtORF; symbiont ITS2 rDNA and chloroplast psbA) were visually inspected and compared to holotype sequences from described Pocillopora species (Schmidt-Roach et al., 2013) and recently described symbiont species derived from these coral species (Turnham et al., 2021).&nbsp;The mtORF sequences from the study specimens all had 100% match with the P. damicornis (GenBank Accession numbers OP296503 – OP296521; 100% match to Pocillopora type alpha cf. (Schmidt-Roach et al., 2013), GenBank accession numbers JX985598 and JX985606).&nbsp;The ITS2 rDNA profiles as well as sequences matched previously described Cladocopium type ‘C1b-c, 42a’ from Heron Reef (Sampayo et al. 2007). For the psbA, 4 out of 24 samples returned poor sequence reads (2 from each environment). The remaining 20 psbA sequences were aligned with inclusion of reference sequences for recently described Cladocopium latusorum and C. pacificum (GenBank Accession numbers MW819755 - MW819779 and MW861711 - MW861727, respectively) as well as the sequence for C. goreaii (Turnham et al., 2021). Sequences were aligned using Geneious (MAFFT), checked and adjusted by eye, which was particularly important given the large number of indels present in the psbA sequence dataset. All sequences were truncated to the 7.4-Forw and 7.8-Rev primer binding sites, given that no differences were seen in the sequence regions extending outward to the psbA coding region psbAFor1/psbARev1 binding sites. The truncates sequences were submitted to GenBank under accession numbers OP279755 – OP279774 (https://www.ncbi.nlm.nih.gov/popset/2310683771).&nbsp; &nbsp;All indels were adjusted to represent a single base change, using gaps as a 5th character state, in subsequent phylogenetic analyses. A psbA (partial coding, and entire non-coding region) maximum parsimony majority-rule phylogeny was constructed using a heuristic search with bootstrap values calculated (TBR branch swapping, random addition, 1000 replicates) in PAUP vs. 4.0a (build 169; Turnham et al., 2021).</p>
<p>For more detailed information, please see: Brown et al. (2022).</p>
Specified by the Principal Investigator(s)
<p>See results publication&nbsp;Brown et al., 2022 for details of statistical analyses performed using these data.&nbsp;</p>
<p>The analysis code package&nbsp;for 'Environmental memory gained from exposure to extreme pCO2 variability promotes coral cellular acid-base homeostasis" published as&nbsp;Brown (2022, doi: 10.5281/zenodo.7373705) which is a publication of github repository&nbsp;<a href="https://github.com/imkristenbrown/pCO2-variability-promotes-coral-cellular-acid-base-homeostasis">https://github.com/imkristenbrown/pCO2-variability-promotes-coral-cellular-acid-base-homeostasis</a>.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact