http://lod.bco-dmo.org/id/dataset/886196
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-12-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.
2023-01-31
publication
2023-01-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-01-31
publication
https://doi.org/10.26008/1912/bco-dmo.886196.1
Hollie Putnam
University of Rhode Island
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Strand, E., Putnam, H. (2023) Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.886196.1 [access date]
Thermal Stress Experiment: Experimental Tank Conditions Dataset Description: Methods and Sampling: <h3>Cultivation of coral nubbins:From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species&nbsp;<em>M. capitata</em>&nbsp;and&nbsp;<em>P. acuta</em>&nbsp;were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. The 30 glued nubbins of each genotype were then randomly distributed among six tanks (~32 liters; 48.3 cm by 38.1 cm by 17.8 cm;&nbsp;<em>L</em>&nbsp;×&nbsp;<em>W</em>&nbsp;×&nbsp;<em>H</em>), leaving five replicates per genotype in each tank for a total of 40 coral nubbins per tank and 120 coral nubbins per species. Tanks were placed in a flow-through system that had a steady supply of water directly from Kāne‘ohe Bay with an average flow rate of (173.8 ± 73 liters hour−1, mean ± SD). Each tank was fitted with a submersible pump (Hydor 200 gph), a HOBO Water Temp Pro temperature logger (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.), an Apex temperature probe (Neptune Systems), and two heaters (Aqueon 300-W Heater set to 31°C and DaToo 300-W Glass Heater set to 34°C). The temperature in the tanks was controlled by powering off and on the heaters based on set points in the Apex aquarium controller (Neptune Systems). Light was set for a 12-hour light/12-hour dark cycle using Arctic T247 lights (Ocean Revive).</h3>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756623 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756623
completed
Hollie Putnam
University of Rhode Island
808-554-1462
120 Flagg Rd.
Kingston
RI
02881
USA
hputnam@uri.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
PPFD_Time
Tank
Position
PPFD
Avg_flowrate
HOBO_Time
HOBO_Temperature
Calibration_date
Discrete_measurement_time
Treatment
Temperature
pH_MV
Salinity
Conductivity
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
submersible pump (Hydor 200 gph)
Apex temperature probe (Neptune Systems)
theme
None, User defined
date
time of day
tank
sample description
PAR
flow rate
water temperature at measurement depth
treatment
pH
salinity
conductivity
featureType
BCO-DMO Standard Parameters
Biospherical PAR sensor
digital thermometer
Multi Parameter Portable Meter
Onset HOBO Pro v2 temperature logger
Pump
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration
https://sites.rutgers.edu/coralbase/
NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration
<p>NSF Abstract:</p>
<p>The remarkable success of coral reefs is explained by interactions of the coral animal with its symbiotic microbiome that is comprised of photosynthetic algae and bacteria. This total organism, or "holobiont", enables high ecosystem biodiversity and productivity in coral reefs. These ecosystems are, however, under threat from a rapidly changing environment. This project aims to integrate information from the cellular to organismal level to identify key mechanisms of adaptation and acclimatization to environmental stress. Specific areas to be investigated include the role of symbionts and of epigenetics (molecular "marks" on coral DNA that regulate gene expression). These aspects will be studied in Hawaiian corals to determine whether they explain why some individuals are sensitive or resistant to environmental perturbation. Results from the proposed project will also provide significant genomic resources that will contribute to fundamental understanding of how complex biological systems generate emergent (i.e., unexpected) properties when faced with fluctuating environments. Broader impacts will extend beyond scientific advancements to include postdoctoral and student training in Science, Technology, Engineering and Mathematics (STEM). Data generated in the project will be used to train university students and do public outreach through live videos of experimental work, and short stop-action animations for topics such as symbiosis, genomics, epigenetics, inheritance, and adaptation. The research approaches and results will be shared with the public in Hawaii through the Hawaii Institute of Marine Biology education department and presentations at Hawaiian hotels, as well as at Rutgers University through its 4-H Rutgerscience Saturdays and 4-H Rutgers Summer Science Programs.</p>
<p>Symbiosis is a complex and ecologically integrated interaction between organisms that provides emergent properties key to their survival. Such is the case for the relationship between reef-building corals and their microbiome, a meta-organism, where nutritional and biogeochemical recycling provide the necessary benefits that fuel high reef productivity and calcification. The rapid warming and acidification of our oceans threatens this symbiosis. This project addresses how relatively stress resistant and stress sensitive corals react to the environmental perturbations of increased temperature and reduced pH. It utilizes transcriptomic, epigenetic, and microbial profiling approaches, to elucidate how corals respond to environmental challenges. In addition to this profiling, work by the BSF Israeli partner will implement powerful analytical techniques such as network theory to detect key transcriptional hubs in meta-organisms and quantify biological integration. This work will generate a stress gene inventory for two ecologically important coral species and a (epi)genome and microbiome level of understanding of how they respond to the physical environment. Acknowledgment of a role for epigenetic mechanisms in corals overturns the paradigm of hardwired genetic control and highlights the interplay of genetic and epigenetic variation that may result in emergent evolutionary and ecologically relevant properties with implications for the future of reefs. Furthermore, clarifying the joint contribution of the microbiome and host in response to abiotic change will provide an important model in metazoan host-microbiome biotic interactions.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Holobiont Integration
largerWorkCitation
project
eng; USA
oceans
2023-01-31
Hawaii, Rhode Island, New Jersey, Israel
0
BCO-DMO catalogue of parameters from Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/886637.rdf
Name: Date
Units: unitless
Description: <p>Date of measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/886638.rdf
Name: PPFD_Time
Units: unitless
Description: <p>Time of light measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/886639.rdf
Name: Tank
Units: unitless
Description: <p>Tank ID </p>
http://lod.bco-dmo.org/id/dataset-parameter/886640.rdf
Name: Position
Units: unitless
Description: <p>Position in the tank of light measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/886641.rdf
Name: PPFD
Units: nm (nanometers)
Description: <p>PAR light value measured </p>
http://lod.bco-dmo.org/id/dataset-parameter/886642.rdf
Name: Avg_flowrate
Units: mL/seconds
Description: <p>Average flow rate measured (n=2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/886643.rdf
Name: HOBO_Time
Units: unitless
Description: <p>Time of temperature measurement from HOBO logger</p>
http://lod.bco-dmo.org/id/dataset-parameter/886644.rdf
Name: HOBO_Temperature
Units: degrees Celsius (°C)
Description: <p>Temperature measurement from HOBO logger</p>
http://lod.bco-dmo.org/id/dataset-parameter/886645.rdf
Name: Calibration_date
Units: unitless
Description: <p>Date of calibration to Tris measurement </p>
http://lod.bco-dmo.org/id/dataset-parameter/886646.rdf
Name: Discrete_measurement_time
Units: unitless
Description: <p>Time of the discrete measurement (Temperature, pH.MV, Salinity, Conductivity)</p>
http://lod.bco-dmo.org/id/dataset-parameter/886647.rdf
Name: Treatment
Units: unitless
Description: <p>High or ambient temperature treatment </p>
http://lod.bco-dmo.org/id/dataset-parameter/886648.rdf
Name: Temperature
Units: degrees Celsius (°C)
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/886649.rdf
Name: pH_MV
Units: millivolts
Description: <p>pH value</p>
http://lod.bco-dmo.org/id/dataset-parameter/886650.rdf
Name: Salinity
Units: parts per thousand (ppt)
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/886651.rdf
Name: Conductivity
Units: millisemens
Description: <p>Conductivity</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6171974
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/8a467436-ffe8-5741-85ac-ca3c7157f744/content
download
https://doi.org/10.26008/1912/bco-dmo.886196.1
download
onLine
dataset
<h3>Cultivation of coral nubbins:From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species&nbsp;<em>M. capitata</em>&nbsp;and&nbsp;<em>P. acuta</em>&nbsp;were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. The 30 glued nubbins of each genotype were then randomly distributed among six tanks (~32 liters; 48.3 cm by 38.1 cm by 17.8 cm;&nbsp;<em>L</em>&nbsp;×&nbsp;<em>W</em>&nbsp;×&nbsp;<em>H</em>), leaving five replicates per genotype in each tank for a total of 40 coral nubbins per tank and 120 coral nubbins per species. Tanks were placed in a flow-through system that had a steady supply of water directly from Kāne‘ohe Bay with an average flow rate of (173.8 ± 73 liters hour−1, mean ± SD). Each tank was fitted with a submersible pump (Hydor 200 gph), a HOBO Water Temp Pro temperature logger (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.), an Apex temperature probe (Neptune Systems), and two heaters (Aqueon 300-W Heater set to 31°C and DaToo 300-W Glass Heater set to 34°C). The temperature in the tanks was controlled by powering off and on the heaters based on set points in the Apex aquarium controller (Neptune Systems). Light was set for a 12-hour light/12-hour dark cycle using Arctic T247 lights (Ocean Revive).</h3>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
PI Supplied Instrument Name: MQ-510 Full Spectrum Underwater Quantum Meter, Apogee PI Supplied Instrument Description:underwater cosine light sensor and meter (MQ-510 Full Spectrum Underwater Quantum Meter, Apogee) Instrument Name: Biospherical PAR sensor Instrument Short Name: Instrument Description: An irradiance sensor designed to measure Photosynthetically Active Radiation (PAR), manufactured by Biospherical Instruments Inc.
PI Supplied Instrument Name: PI Supplied Instrument Description:handheld digital certified thermometer (Control Company accuracy, ± 0.05°C; resolution, 0.001°C) Instrument Name: digital thermometer Instrument Short Name: Instrument Description: An instrument that measures temperature digitally.
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
MQ-510 Full Spectrum Underwater Quantum Meter, Apogee
PI Supplied Instrument Name: MQ-510 Full Spectrum Underwater Quantum Meter, Apogee Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
PI Supplied Instrument Name: PI Supplied Instrument Description:HOBO Water Temp Pro temperature loggers (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.) Instrument Name: Onset HOBO Pro v2 temperature logger Instrument Short Name:HOBO U22-001 Instrument Description: The HOBO Water Temp Pro v2 temperature logger, manufactured by Onset Computer Corporation, has 12-bit resolution and a precision sensor for ±0.2°C accuracy over a wide temperature range. It is designed for extended deployment in fresh or salt water.
Operation range: -40° to 70°C (-40° to 158°F) in air; maximum sustained temperature of 50°C (122°F) in water
Accuracy: 0.2°C over 0° to 50°C (0.36°F over 32° to 122°F)
Resolution: 0.02°C at 25°C (0.04°F at 77°F)
Response time: (90%) 5 minutes in water; 12 minutes in air moving 2 m/sec (typical)
Stability (drift): 0.1°C (0.18°F) per year
Real-time clock: ± 1 minute per month 0° to 50°C (32° to 122°F)
Additional information (http://www.onsetcomp.com/)
Onset Computer Corporation
470 MacArthur Blvd
Bourne, MA 02532 Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
submersible pump (Hydor 200 gph)
submersible pump (Hydor 200 gph)
PI Supplied Instrument Name: submersible pump (Hydor 200 gph) Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Apex temperature probe (Neptune Systems)
Apex temperature probe (Neptune Systems)
PI Supplied Instrument Name: Apex temperature probe (Neptune Systems) Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/