http://lod.bco-dmo.org/id/dataset/886427 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-01-06 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. 2023-01-31 publication 2023-01-31 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-02-01 publication https://doi.org/10.26008/1912/bco-dmo.886427.1 Hollie Putnam University of Rhode Island principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Strand, E., Putnam, H. (2023) Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.886427.1 [access date] Thermal Stress Experiment: Untargeted Metabolites Dataset Description: Methods and Sampling: &lt;p&gt;From the waters of &lt;strong&gt;Kāne‘ohe Bay, HI&lt;/strong&gt;, four colonies of each coral species &lt;em&gt;M. capitata&lt;/em&gt; and &lt;em&gt;P. acuta&lt;/em&gt; were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the &lt;strong&gt;Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI&lt;/strong&gt;, and hot-glued to labeled plugs. (21N 157 W ; depth 1m).&lt;/p&gt; &lt;p&gt;Metabolite extraction and analysis conducted at Rutgers University.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral sampling:&lt;/strong&gt; Sampling of the nubbins began after the onset of color score divergence between the treatment groups. Three sampling points were selected on 22 May 2019 (T1), 3 June 2019 (T3), and 7 June 2019 (T5) on the basis of reaching maximum treatment temperature, where the coral color score began to diverge by treatment and where the coral color score differences were maximized between treatments within species within the experimental time frame. Coral nubbins were selected for sampling randomly using a random number generator, and color scores were recorded for each nubbin after sampling. Corals were sampled at ~14:30 at each time point by removing them from their treatment, only touching the plastic bases and inserting them into sterile Whirl-Paks that were immediately submerged in liquid nitrogen and transferred to −80°C until metabolite extraction.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolite extraction from coral nubbins:&lt;/strong&gt; Metabolites were extracted using a protocol optimized for water-soluble polar metabolite analysis on LC-MS. The extraction buffer was a solution of 40:40:20 (methanol:acetonitrile:water) (v/v/v) + 0.1 M formic acid. The extraction buffer was stored at −20°C before usage. Immediately preceding the metabolite extraction, 1 ml of extraction buffer was added to a 2-ml glass Dounce homogenizer that had chilled on ice. Pieces of the −80°C preserved nubbins were then clipped, weighed, and added to the cold extraction buffer in the Dounce and left to incubate for 5 min. The pestle of the Dounce was then used to homogenize the coral tissue until there was a visible accumulation of coral skeleton at the bottom of the Dounce and the homogenate was visibly pigmented. An additional 500-μl aliquot of cold 40:40:20 + 0.1M formic acid extraction buffer was then used to rinse down the sides of the Dounce and pestle. The total 1.5-ml volume was then strained through a sterile 100-μm cell strainer into a 50-ml receptacle. There was a visible amount of skeleton collected in the strainer. The rest of the homogenate was then transferred to a 1.5-ml Eppendorf tube, vortexed for 10 s, and then centrifuged for 10 min at 16,000g&amp;nbsp;at 4°C. After centrifugation, there was a pellet at the bottom of the tube. A final 500-μl aliquot of the homogenate was then pipetted to a second clean Eppendorf tube, to which 44 μl of 15% NH4HCO3&amp;nbsp;was added to neutralize the acid in the buffer. This was the final extract and was ready to be loaded to instrument vials for analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Cultivation of Aiptasia:&lt;/strong&gt; The animals were held at 27°C under a 12-hour light/12-hour dark cycle at 25 μmol photons m−2&amp;nbsp;s−1. All individuals were from the clonal population CC7, which naturally contains the algal symbionts of the Symbiodiniaceae clade A species&amp;nbsp;Symbiodinium linuchae&amp;nbsp;(Sunagawa et al. 2009). CC7 animals were made aposymbiotic as previously described (Xiang et al. 2013). This resulted in the strain CC7-Apo. CC7-Apo animals subsequently exposed to algae of the clonal axenic strain SSB01 (Symbiodiniaceae clade B species&amp;nbsp;Breviolum minutum) (Baumgarten et al. 2015) and cultured under standard conditions. After these anemone strains were created, each strain was continued and propagated though asexual reproduction in long-term culture for &amp;gt;1 year before experimentation. By using long-term, stable anemone cultures, we intended to minimize the effects of the stress on the animals required to generate the CC7-Apo and CC7-SSB01 anemones that were used for metabolomic analysis. Aiptasia were aliquoted individually to the 1.5-ml Eppendorf tubes in 500 μl of artificial seawater, flash-frozen, and stored at −80°C until processing at Rutgers University.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolite extraction from Aiptasia:&lt;/strong&gt; Metabolites were extracted using a protocol optimized for water-soluble polar metabolite analysis on LCMS. The extraction buffer used was a solution of 40:40:20 (methanol:acetonitrile:water) (v/v/v) + 0.1 M formic acid and was stored at −20°C before usage. CC7-Apo and CC7-SSB01 animals were removed from the artificial seawater storage, and intragroup animals were pooled to produce six replicates, each having an input weight of 25 mg in a 1.5-ml Eppendorf tube. Replicates were kept on dry ice before extraction. A total of 500 μl of extraction buffer was added; then, samples were vortexed for 10 s and transferred to crushed ice to incubate for 10 min. Samples were then centrifuged for 10 min, and the supernatant was transferred to a 1.5-ml Eppendorf tube; the procedure was repeated for a second round of extraction. A total of 1 ml of supernatant was then centrifuged, and a final 500-μl aliquot of the homogenate was pipetted to a clean Eppendorf tube, to which 44 μl of 15% NH4HCO3&amp;nbsp;was added to neutralize the acid in the buffer. This was the final extract and was ready to be loaded to instrument vials for analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;UHPLC conditions:&lt;/strong&gt; The HILIC separation was performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) with XBridge BEH Amide column (150 mm by 2.1 mm, 2.5-μm particle size; Waters, Milford, MA) using a gradient of solvent A [95%:5% H2O:acetonitrile with 20 mM acetic acid and 40 mM ammonium hydroxide (pH 9.4)] and solvent B [20%:80% H2O:acetonitrile with 20 mM acetic acid and 40 mM ammonium hydroxide (pH 9.4)]. The gradient was 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B. The flow rate was 300 μl/min. The injection volume was 5 μl, and the column temperature was 25°C. The autosampler temperature was set to 4°C, and the injection volume was 5 μl. Full-scan MS: The full-scan MS analysis was performed on a Thermo Fisher Scientific Q Exactive Plus with a HESI source, which was set to a spray voltage of −2.7 kV under negative mode and 3.5 kV under positive mode. The sheath, auxiliary, and sweep gas flow rates were 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and aux gas heater was 360°C. The S-lens radio frequency (RF) level was 45. The&amp;nbsp;m/z&amp;nbsp;scan range was set to 72 to 1000&amp;nbsp;m/z&amp;nbsp;under both positive and negative ionization mode. The automatic gain control (AGC) target was set to 3e6, and the maximum injection time (IT) was 200 ms. The resolution was set to 70,000.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Parallel reaction monitoring MS:&lt;/strong&gt; The MS2&amp;nbsp;spectra generation was performed on a Thermo Fisher Scientific Q Exactive Plus with a HESI source, which was set to a spray voltage of −2.7 kV under negative mode and 3.5 kV under positive mode. The sheath, auxiliary, and sweep gas flow rates were 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and aux gas heater was 360°C. The S-lens RF level was 45. The&amp;nbsp;m/z&amp;nbsp;scan ranges were specified for the four dipeptides and monitored for the full 22-min run time. The AGC target was set to 2 × 105, and the maximum IT was 100 ms. The resolution was set to 17,500. The isolation window was set to 2.0&amp;nbsp;m/z. Collision energy was set to a stepwise 30, 50, and 80 normalized collision energy (NCE). Our results suggest that a single MS2&amp;nbsp;spectrum may contain irrelevant&amp;nbsp;m/z&amp;nbsp;signals and the pseudospectrum, generated by correlating multiple MS2&amp;nbsp;spectra to the MS1-extracted ion chromatogram, is an effective approach to “clean” the MS2&amp;nbsp;spectrum. The pseudospectra were generated using a modified version of COVINA (Su et al. 2020).&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Synthesis of standards:&lt;/strong&gt; The dipeptide and tripeptide standards were synthesized and purified to 95% purity by GenScript USA (Piscataway, NJ). All standards were shipped at 25°C and stored at −20°C until they were prepared for analysis on the mass spectrometer. Parallel reaction monitoring was used to generate the MS2&amp;nbsp;spectra for comparison with samples. A total of 12 pmol of each standard was used in the analysis.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756623 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756623 completed Hollie Putnam University of Rhode Island 808-554-1462 120 Flagg Rd. 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Area_Symbiotic_3_raw_F12_set3 Area_Symbiotic_4_raw_F13_set3 Area_Symbiotic_5_raw_F14_set3 Area_Symbiotic_6_raw_F15_set3 Ratio_Asymbiotic_Symbiotic_set3 Log2FoldChange_Asymbiotic_Symbiotic_set3 Pvalue_Asymbiotic_Symbiotic_set3 Adj_Pvalue_Asymbiotic_Symbiotic_set3 2-ml glass Dounce homogenizer Liquid chromatography mass spectrometer Thermo Fisher Scientific Q Exactive Plus anquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) theme None, User defined sample identification molecular weight time_elapsed count ratio logarithmic_transformation p-value featureType BCO-DMO Standard Parameters Centrifuge Homogenizer Mass Spectrometer Mass Spectrometer Ultra-high-performance liquid chromatography instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration https://sites.rutgers.edu/coralbase/ NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration <p>NSF Abstract:</p> <p>The remarkable success of coral reefs is explained by interactions of the coral animal with its symbiotic microbiome that is comprised of photosynthetic algae and bacteria. This total organism, or "holobiont", enables high ecosystem biodiversity and productivity in coral reefs. These ecosystems are, however, under threat from a rapidly changing environment. This project aims to integrate information from the cellular to organismal level to identify key mechanisms of adaptation and acclimatization to environmental stress. Specific areas to be investigated include the role of symbionts and of epigenetics (molecular "marks" on coral DNA that regulate gene expression). These aspects will be studied in Hawaiian corals to determine whether they explain why some individuals are sensitive or resistant to environmental perturbation. Results from the proposed project will also provide significant genomic resources that will contribute to fundamental understanding of how complex biological systems generate emergent (i.e., unexpected) properties when faced with fluctuating environments. Broader impacts will extend beyond scientific advancements to include postdoctoral and student training in Science, Technology, Engineering and Mathematics (STEM). Data generated in the project will be used to train university students and do public outreach through live videos of experimental work, and short stop-action animations for topics such as symbiosis, genomics, epigenetics, inheritance, and adaptation. The research approaches and results will be shared with the public in Hawaii through the Hawaii Institute of Marine Biology education department and presentations at Hawaiian hotels, as well as at Rutgers University through its 4-H Rutgerscience Saturdays and 4-H Rutgers Summer Science Programs.</p> <p>Symbiosis is a complex and ecologically integrated interaction between organisms that provides emergent properties key to their survival. Such is the case for the relationship between reef-building corals and their microbiome, a meta-organism, where nutritional and biogeochemical recycling provide the necessary benefits that fuel high reef productivity and calcification. The rapid warming and acidification of our oceans threatens this symbiosis. This project addresses how relatively stress resistant and stress sensitive corals react to the environmental perturbations of increased temperature and reduced pH. It utilizes transcriptomic, epigenetic, and microbial profiling approaches, to elucidate how corals respond to environmental challenges. In addition to this profiling, work by the BSF Israeli partner will implement powerful analytical techniques such as network theory to detect key transcriptional hubs in meta-organisms and quantify biological integration. This work will generate a stress gene inventory for two ecologically important coral species and a (epi)genome and microbiome level of understanding of how they respond to the physical environment. Acknowledgment of a role for epigenetic mechanisms in corals overturns the paradigm of hardwired genetic control and highlights the interplay of genetic and epigenetic variation that may result in emergent evolutionary and ecologically relevant properties with implications for the future of reefs. Furthermore, clarifying the joint contribution of the microbiome and host in response to abiotic change will provide an important model in metazoan host-microbiome biotic interactions.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Holobiont Integration largerWorkCitation project eng; USA oceans 2023-01-31 Hawaii, Rhode Island, New Jersey, Israel 0 BCO-DMO catalogue of parameters from Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/888142.rdf Name: Ionization Units: unitless Description: <p>The target group (M. capitata, Aiptasia, or P. acuta) and ionization (positive or negative)</p> http://lod.bco-dmo.org/id/dataset-parameter/888143.rdf Name: Number Units: unitless Description: <p>Ionization groupID</p> http://lod.bco-dmo.org/id/dataset-parameter/888144.rdf Name: MolecularWeight Units: grams per mole Description: <p>Molecular weight of identified compound</p> http://lod.bco-dmo.org/id/dataset-parameter/888145.rdf Name: RT_min Units: unitless Description: <p>Minimum retention time in minutes</p> http://lod.bco-dmo.org/id/dataset-parameter/888146.rdf Name: Area_Blank1_raw_F73_set1 Units: ion count Description: <p>Ion counts for each metabolite in blank samples (ion count) </p> http://lod.bco-dmo.org/id/dataset-parameter/888147.rdf Name: Area_Blank2_raw_F74_set1 Units: ion count Description: <p>Ion counts for each metabolite in blank samples (ion count) </p> http://lod.bco-dmo.org/id/dataset-parameter/888148.rdf Name: Area_Blank3_raw_F75_set1 Units: ion count Description: <p>Ion counts for each metabolite in blank samples (ion count) </p> http://lod.bco-dmo.org/id/dataset-parameter/888149.rdf Name: Area_1084_T1_A_raw_F5_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888150.rdf Name: Area_1124_T1_A_raw_F7_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888151.rdf Name: Area_1462_T1_A_raw_F26_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888152.rdf Name: Area_1477_T1_A_raw_F28_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888153.rdf Name: Area_1535_T1_A_raw_F29_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888154.rdf Name: Area_1604_T1_A_raw_F36_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888155.rdf Name: Area_1609_T1_A_raw_F37_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888156.rdf Name: Area_1772_T1_A_raw_F46_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888157.rdf Name: Area_2210_T1_A_raw_F54_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888158.rdf Name: Area_2519_T1_A_raw_F59_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888159.rdf Name: Area_2871_T1_A_raw_F66_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888160.rdf Name: Area_1078_T1_H_raw_F4_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888161.rdf Name: Area_1142_T1_H_raw_F8_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888162.rdf Name: Area_1178_T1_H_raw_F10_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888163.rdf Name: Area_1442_T1_H_raw_F24_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888164.rdf Name: Area_1587_T1_H_raw_F34_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888165.rdf Name: Area_1710_T1_H_raw_F38_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888166.rdf Name: Area_2019_T1_H_raw_F49_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888167.rdf Name: Area_2023_T1_H_raw_F50_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888168.rdf Name: Area_2750_T1_H_raw_F64_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888169.rdf Name: Area_2756_T1_H_raw_F65_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888170.rdf Name: Area_2878_T1_H_raw_F68_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888171.rdf Name: Area_2985_T1_H_raw_F69_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888172.rdf Name: Area_1048_T3_A_raw_F1_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888173.rdf Name: Area_1093_T3_A_raw_F6_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888174.rdf Name: Area_1271_T3_A_raw_F15_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888175.rdf Name: Area_1302_T3_A_raw_F17_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888176.rdf Name: Area_1308_T3_A_raw_F19_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888177.rdf Name: Area_1427_T3_A_raw_F23_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888178.rdf Name: Area_1544_T3_A_raw_F30_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888179.rdf Name: Area_1595_T3_A_raw_F35_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888180.rdf Name: Area_1736_T3_A_raw_F43_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888181.rdf Name: Area_2413_T3_A_raw_F58_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888182.rdf Name: Area_2530_T3_A_raw_F60_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888183.rdf Name: Area_2741_T3_A_raw_F63_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888184.rdf Name: Area_2996_T3_A_raw_F70_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888185.rdf Name: Area_1239_T3_H_raw_F12_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888186.rdf Name: Area_1294_T3_H_raw_F16_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888187.rdf Name: Area_1307_T3_H_raw_F18_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888188.rdf Name: Area_1327_T3_H_raw_F20_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888189.rdf Name: Area_1447_T3_H_raw_F25_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888190.rdf Name: Area_1725_T3_H_raw_F40_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888191.rdf Name: Area_1726_T3_H_raw_F41_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888192.rdf Name: Area_1730_T3_H_raw_F42_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888193.rdf Name: Area_2058_T3_H_raw_F51_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888194.rdf Name: Area_2183_T3_H_raw_F53_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888195.rdf Name: Area_2740_T3_H_raw_F62_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888196.rdf Name: Area_2998_T3_H_raw_F71_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888197.rdf Name: Area_1052_T5_A_raw_F2_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888198.rdf Name: Area_1161_T5_A_raw_F9_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888199.rdf Name: Area_1236_T5_A_raw_F11_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888200.rdf Name: Area_1258_T5_A_raw_F13_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888201.rdf Name: Area_1426_T5_A_raw_F22_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888202.rdf Name: Area_1558_T5_A_raw_F31_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888203.rdf Name: Area_1574_T5_A_raw_F33_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888204.rdf Name: Area_1721_T5_A_raw_F39_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888205.rdf Name: Area_1740_T5_A_raw_F44_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888206.rdf Name: Area_2172_T5_A_raw_F52_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888207.rdf Name: Area_2212_T5_A_raw_F55_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888208.rdf Name: Area_2734_T5_A_raw_F61_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888209.rdf Name: Area_2874_T5_A_raw_F67_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888210.rdf Name: Area_1063_T5_H_raw_F3_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888211.rdf Name: Area_1262_T5_H_raw_F14_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888212.rdf Name: Area_1341_T5_H_raw_F21_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888213.rdf Name: Area_1475_T5_H_raw_F27_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888214.rdf Name: Area_1565_T5_H_raw_F32_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888215.rdf Name: Area_1744_T5_H_raw_F45_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888216.rdf Name: Area_1773_T5_H_raw_F47_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888217.rdf Name: Area_2005_T5_H_raw_F48_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888218.rdf Name: Area_2306_T5_H_raw_F56_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888219.rdf Name: Area_2396_T5_H_raw_F57_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888220.rdf Name: Area_2998_T5_H_raw_F72_set1 Units: ion count Description: <p>Set 1, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888221.rdf Name: Ratio_T1_H_T1_A_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888222.rdf Name: Ratio_T3_A_T1_A_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888223.rdf Name: Ratio_T3_H_T1_H_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888224.rdf Name: Ratio_T5_A_T1_A_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888225.rdf Name: Ratio_T5_H_T1_H_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888226.rdf Name: Ratio_T3_H_T3_A_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888227.rdf Name: Ratio_T5_H_T5_A_set1 Units: ion count Description: <p>Set 1, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888228.rdf Name: Log2FoldChange_T1_H_T1_A_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888229.rdf Name: Log2FoldChange_T3_A_T1_A_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888230.rdf Name: Log2FoldChange_T3_H_T1_H_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888231.rdf Name: Log2FoldChange_T5_A_T1_A_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888232.rdf Name: Log2FoldChange_T5_H_T1_H_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888233.rdf Name: Log2FoldChange_T3_H_T3_A_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888234.rdf Name: Log2FoldChange_T5_H_T5_A_set1 Units: ion count Description: <p>Set 1, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888235.rdf Name: Pvalue_T1_H_T1_A_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888236.rdf Name: Pvalue_T3_A_T1_A_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888237.rdf Name: Pvalue_T3_H_T1_H_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888238.rdf Name: Pvalue_T5_A_T1_A_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888239.rdf Name: Pvalue_T5_H_T1_H_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888240.rdf Name: Pvalue_T3_H_T3_A_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888241.rdf Name: Pvalue_T5_H_T5_A_set1 Units: ion count Description: <p>Set 1, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888242.rdf Name: Adj_Pvalue_T1_H_T1_A_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888243.rdf Name: Adj_Pvalue_T3_A_T1_A_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888244.rdf Name: Adj_Pvalue_T3_H_T1_H_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888245.rdf Name: Adj_Pvalue_T5_A_T1_A_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888246.rdf Name: Adj_Pvalue_T5_H_T1_H_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888247.rdf Name: Adj_Pvalue_T3_H_T3_A_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888248.rdf Name: Adj_Pvalue_T5_H_T5_A_set1 Units: ion count Description: <p>Set 1, adjusted p-value associated with each timepoint x treatment interaction (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888249.rdf Name: Area_1115_T1_A_raw_F8_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888250.rdf Name: Area_1181_T1_A_raw_F10_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888251.rdf Name: Area_1299_T1_A_raw_F22_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888252.rdf Name: Area_1310_T1_A_raw_F23_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888253.rdf Name: Area_1319_T1_A_raw_F25_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888254.rdf Name: Area_1415_T1_A_raw_F27_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888255.rdf Name: Area_1632_T1_A_raw_F39_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888256.rdf Name: Area_1637_T1_A_raw_F40_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888257.rdf Name: Area_1651_T1_A_raw_F43_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888258.rdf Name: Area_2081_T1_A_raw_F53_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888259.rdf Name: Area_2540_T1_A_raw_F63_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888260.rdf Name: Area_2978_T1_A_raw_F70_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888261.rdf Name: Area_1090_T1_H_raw_F5_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888262.rdf Name: Area_1196_T1_H_raw_F11_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888263.rdf Name: Area_1216_T1_H_raw_F15_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888264.rdf Name: Area_1425_T1_H_raw_F28_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888265.rdf Name: Area_1481_T1_H_raw_F30_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888266.rdf Name: Area_1702_T1_H_raw_F45_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888267.rdf Name: Area_1751_T1_H_raw_F46_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888268.rdf Name: Area_1999_T1_H_raw_F47_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888269.rdf Name: Area_2022_T1_H_raw_F50_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888270.rdf Name: Area_2409_T1_H_raw_F60_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888271.rdf Name: Area_2511_T1_H_raw_F61_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888272.rdf Name: Area_2866_T1_H_raw_F68_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888273.rdf Name: Area_1044_T3_A_raw_F1_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888274.rdf Name: Area_1212_T3_A_raw_F13_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888275.rdf Name: Area_1213_T3_A_raw_F14_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888276.rdf Name: Area_1230_T3_A_raw_F17_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888277.rdf Name: Area_1297_T3_A_raw_F21_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888278.rdf Name: Area_1482_T3_A_raw_F31_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888279.rdf Name: Area_1638_T3_A_raw_F41_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888280.rdf Name: Area_1676_T3_A_raw_F44_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888281.rdf Name: Area_2010_T3_A_raw_F49_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888282.rdf Name: Area_2864_T3_A_raw_F67_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888283.rdf Name: Area_2872_T3_A_raw_F69_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888284.rdf Name: Area_3000_T3_A_raw_F72_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888285.rdf Name: Area_1222_T3_H_raw_F16_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888286.rdf Name: Area_1232_T3_H_raw_F18_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888287.rdf Name: Area_1268_T3_H_raw_F19_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888288.rdf Name: Area_1570_T3_H_raw_F32_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888289.rdf Name: Area_1579_T3_H_raw_F33_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888290.rdf Name: Area_1586_T3_H_raw_F36_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888291.rdf Name: Area_1640_T3_H_raw_F42_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888292.rdf Name: Area_2001_T3_H_raw_F48_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888293.rdf Name: Area_2156_T3_H_raw_F54_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888294.rdf Name: Area_2391_T3_H_raw_F58_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888295.rdf Name: Area_2397_T3_H_raw_F59_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888296.rdf Name: Area_2552_T3_H_raw_F64_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888297.rdf Name: Area_1068_T5_A_raw_F3_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888298.rdf Name: Area_1079_T5_A_raw_F4_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888299.rdf Name: Area_1092_T5_A_raw_F6_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888300.rdf Name: Area_1103_T5_A_raw_F7_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888301.rdf Name: Area_1156_T5_A_raw_F9_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888302.rdf Name: Area_1278_T5_A_raw_F20_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888303.rdf Name: Area_1344_T5_A_raw_F26_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888304.rdf Name: Area_1583_T5_A_raw_F35_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888305.rdf Name: Area_2072_T5_A_raw_F51_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888306.rdf Name: Area_2194_T5_A_raw_F56_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888307.rdf Name: Area_2388_T5_A_raw_F57_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888308.rdf Name: Area_2854_T5_A_raw_F65_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888309.rdf Name: Area_1060_T5_H_raw_F2_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888310.rdf Name: Area_1208_T5_H_raw_F12_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888311.rdf Name: Area_1317_T5_H_raw_F24_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888312.rdf Name: Area_1454_T5_H_raw_F29_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888313.rdf Name: Area_1580_T5_H_raw_F34_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888314.rdf Name: Area_1599_T5_H_raw_F37_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888315.rdf Name: Area_1605_T5_H_raw_F38_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888316.rdf Name: Area_2076_T5_H_raw_F52_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888317.rdf Name: Area_2169_T5_H_raw_F55_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888318.rdf Name: Area_2528_T5_H_raw_F62_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888319.rdf Name: Area_2855_T5_H_raw_F66_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888320.rdf Name: Area_2995_T5_H_raw_F71_set2 Units: ion count Description: <p>Set 2, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888321.rdf Name: Ratio_T1_H_T1_A_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888322.rdf Name: Ratio_T3_A_T1_A_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888323.rdf Name: Ratio_T3_H_T1_H_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888324.rdf Name: Ratio_T5_A_T1_A_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888325.rdf Name: Ratio_T5_H_T1_H_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888326.rdf Name: Ratio_T3_H_T3_A_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888327.rdf Name: Ratio_T5_H_T5_A_set2 Units: ion count Description: <p>Set 2, Ratio of ion counts for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888328.rdf Name: Log2FoldChange_T1_H_T1_A_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888329.rdf Name: Log2FoldChange_T3_A_T1_A_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888330.rdf Name: Log2FoldChange_T3_H_T1_H_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888331.rdf Name: Log2FoldChange_T5_A_T1_A_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888332.rdf Name: Log2FoldChange_T5_H_T1_H_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888333.rdf Name: Log2FoldChange_T3_H_T3_A_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888334.rdf Name: Log2FoldChange_T5_H_T5_A_set2 Units: ion count Description: <p>Set 2, Log2 fold change for each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888335.rdf Name: Pvalue_T1_H_T1_A_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888336.rdf Name: Pvalue_T3_A_T1_A_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888337.rdf Name: Pvalue_T3_H_T1_H_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888338.rdf Name: Pvalue_T5_A_T1_A_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888339.rdf Name: Pvalue_T5_H_T1_H_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888340.rdf Name: Pvalue_T3_H_T3_A_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888341.rdf Name: Pvalue_T5_H_T5_A_set2 Units: ion count Description: <p>Set 2, p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888342.rdf Name: Adj_Pvalue_T1_H_T1_A_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888343.rdf Name: Adj_Pvalue_T3_A_T1_A_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888344.rdf Name: Adj_Pvalue_T3_H_T1_H_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888345.rdf Name: Adj_Pvalue_T5_A_T1_A_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888346.rdf Name: Adj_Pvalue_T5_H_T1_H_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888347.rdf Name: Adj_Pvalue_T3_H_T3_A_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888348.rdf Name: Adj_Pvalue_T5_H_T5_A_set2 Units: ion count Description: <p>Set 2, adjusted p-value associated with each timepoint x treatment interaction: (Timepoint#, Treatment) / (Timepoint#, Treatment)</p> http://lod.bco-dmo.org/id/dataset-parameter/888349.rdf Name: Area_Asymbiotic_1_raw_F1_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888350.rdf Name: Area_Asymbiotic_2_raw_F2_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888351.rdf Name: Area_Asymbiotic_3_raw_F3_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888352.rdf Name: Area_Asymbiotic_4_raw_F4_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888353.rdf Name: Area_Asymbiotic_5_raw_F5_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888354.rdf Name: Area_Asymbiotic_6_raw_F6_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888355.rdf Name: Area_Blank1_raw_F7_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888356.rdf Name: Area_Blank2_raw_F8_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888357.rdf Name: Area_Blank3_raw_F9_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888358.rdf Name: Area_Symbiotic_1_raw_F10_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888359.rdf Name: Area_Symbiotic_2_raw_F11_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888360.rdf Name: Area_Symbiotic_3_raw_F12_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888361.rdf Name: Area_Symbiotic_4_raw_F13_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888362.rdf Name: Area_Symbiotic_5_raw_F14_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888363.rdf Name: Area_Symbiotic_6_raw_F15_set3 Units: ion count Description: <p>Set 3, Ion counts for each metabolite in coral samples (ion count):  CoralID (one column for each coral individual)</p> http://lod.bco-dmo.org/id/dataset-parameter/888364.rdf Name: Ratio_Asymbiotic_Symbiotic_set3 Units: ion count Description: <p>Set 3, Ratio of ion counts for each interaction: (Asymbiotic) / (Symbiotic)</p> http://lod.bco-dmo.org/id/dataset-parameter/888365.rdf Name: Log2FoldChange_Asymbiotic_Symbiotic_set3 Units: ion count Description: <p>Set 3, Log2 fold change for each interaction:  (Asymbiotic) / (Symbiotic)</p> http://lod.bco-dmo.org/id/dataset-parameter/888366.rdf Name: Pvalue_Asymbiotic_Symbiotic_set3 Units: ion count Description: <p>Set 3, p-value associated with each interaction:  (Asymbiotic) / (Symbiotic)</p> http://lod.bco-dmo.org/id/dataset-parameter/888367.rdf Name: Adj_Pvalue_Asymbiotic_Symbiotic_set3 Units: ion count Description: <p>Set 3, adjusted p-value associated with each interaction:  (Asymbiotic) / (Symbiotic)</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 30225298 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4b1e8c81-7b98-53b7-a510-597f6fc5bd44/content download https://doi.org/10.26008/1912/bco-dmo.886427.1 download onLine dataset &lt;p&gt;From the waters of &lt;strong&gt;Kāne‘ohe Bay, HI&lt;/strong&gt;, four colonies of each coral species &lt;em&gt;M. capitata&lt;/em&gt; and &lt;em&gt;P. acuta&lt;/em&gt; were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the &lt;strong&gt;Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI&lt;/strong&gt;, and hot-glued to labeled plugs. (21N 157 W ; depth 1m).&lt;/p&gt; &lt;p&gt;Metabolite extraction and analysis conducted at Rutgers University.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral sampling:&lt;/strong&gt; Sampling of the nubbins began after the onset of color score divergence between the treatment groups. Three sampling points were selected on 22 May 2019 (T1), 3 June 2019 (T3), and 7 June 2019 (T5) on the basis of reaching maximum treatment temperature, where the coral color score began to diverge by treatment and where the coral color score differences were maximized between treatments within species within the experimental time frame. Coral nubbins were selected for sampling randomly using a random number generator, and color scores were recorded for each nubbin after sampling. Corals were sampled at ~14:30 at each time point by removing them from their treatment, only touching the plastic bases and inserting them into sterile Whirl-Paks that were immediately submerged in liquid nitrogen and transferred to −80°C until metabolite extraction.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolite extraction from coral nubbins:&lt;/strong&gt; Metabolites were extracted using a protocol optimized for water-soluble polar metabolite analysis on LC-MS. The extraction buffer was a solution of 40:40:20 (methanol:acetonitrile:water) (v/v/v) + 0.1 M formic acid. The extraction buffer was stored at −20°C before usage. Immediately preceding the metabolite extraction, 1 ml of extraction buffer was added to a 2-ml glass Dounce homogenizer that had chilled on ice. Pieces of the −80°C preserved nubbins were then clipped, weighed, and added to the cold extraction buffer in the Dounce and left to incubate for 5 min. The pestle of the Dounce was then used to homogenize the coral tissue until there was a visible accumulation of coral skeleton at the bottom of the Dounce and the homogenate was visibly pigmented. An additional 500-μl aliquot of cold 40:40:20 + 0.1M formic acid extraction buffer was then used to rinse down the sides of the Dounce and pestle. The total 1.5-ml volume was then strained through a sterile 100-μm cell strainer into a 50-ml receptacle. There was a visible amount of skeleton collected in the strainer. The rest of the homogenate was then transferred to a 1.5-ml Eppendorf tube, vortexed for 10 s, and then centrifuged for 10 min at 16,000g&amp;nbsp;at 4°C. After centrifugation, there was a pellet at the bottom of the tube. A final 500-μl aliquot of the homogenate was then pipetted to a second clean Eppendorf tube, to which 44 μl of 15% NH4HCO3&amp;nbsp;was added to neutralize the acid in the buffer. This was the final extract and was ready to be loaded to instrument vials for analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Cultivation of Aiptasia:&lt;/strong&gt; The animals were held at 27°C under a 12-hour light/12-hour dark cycle at 25 μmol photons m−2&amp;nbsp;s−1. All individuals were from the clonal population CC7, which naturally contains the algal symbionts of the Symbiodiniaceae clade A species&amp;nbsp;Symbiodinium linuchae&amp;nbsp;(Sunagawa et al. 2009). CC7 animals were made aposymbiotic as previously described (Xiang et al. 2013). This resulted in the strain CC7-Apo. CC7-Apo animals subsequently exposed to algae of the clonal axenic strain SSB01 (Symbiodiniaceae clade B species&amp;nbsp;Breviolum minutum) (Baumgarten et al. 2015) and cultured under standard conditions. After these anemone strains were created, each strain was continued and propagated though asexual reproduction in long-term culture for &amp;gt;1 year before experimentation. By using long-term, stable anemone cultures, we intended to minimize the effects of the stress on the animals required to generate the CC7-Apo and CC7-SSB01 anemones that were used for metabolomic analysis. Aiptasia were aliquoted individually to the 1.5-ml Eppendorf tubes in 500 μl of artificial seawater, flash-frozen, and stored at −80°C until processing at Rutgers University.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolite extraction from Aiptasia:&lt;/strong&gt; Metabolites were extracted using a protocol optimized for water-soluble polar metabolite analysis on LCMS. The extraction buffer used was a solution of 40:40:20 (methanol:acetonitrile:water) (v/v/v) + 0.1 M formic acid and was stored at −20°C before usage. CC7-Apo and CC7-SSB01 animals were removed from the artificial seawater storage, and intragroup animals were pooled to produce six replicates, each having an input weight of 25 mg in a 1.5-ml Eppendorf tube. Replicates were kept on dry ice before extraction. A total of 500 μl of extraction buffer was added; then, samples were vortexed for 10 s and transferred to crushed ice to incubate for 10 min. Samples were then centrifuged for 10 min, and the supernatant was transferred to a 1.5-ml Eppendorf tube; the procedure was repeated for a second round of extraction. A total of 1 ml of supernatant was then centrifuged, and a final 500-μl aliquot of the homogenate was pipetted to a clean Eppendorf tube, to which 44 μl of 15% NH4HCO3&amp;nbsp;was added to neutralize the acid in the buffer. This was the final extract and was ready to be loaded to instrument vials for analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;UHPLC conditions:&lt;/strong&gt; The HILIC separation was performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) with XBridge BEH Amide column (150 mm by 2.1 mm, 2.5-μm particle size; Waters, Milford, MA) using a gradient of solvent A [95%:5% H2O:acetonitrile with 20 mM acetic acid and 40 mM ammonium hydroxide (pH 9.4)] and solvent B [20%:80% H2O:acetonitrile with 20 mM acetic acid and 40 mM ammonium hydroxide (pH 9.4)]. The gradient was 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B. The flow rate was 300 μl/min. The injection volume was 5 μl, and the column temperature was 25°C. The autosampler temperature was set to 4°C, and the injection volume was 5 μl. Full-scan MS: The full-scan MS analysis was performed on a Thermo Fisher Scientific Q Exactive Plus with a HESI source, which was set to a spray voltage of −2.7 kV under negative mode and 3.5 kV under positive mode. The sheath, auxiliary, and sweep gas flow rates were 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and aux gas heater was 360°C. The S-lens radio frequency (RF) level was 45. The&amp;nbsp;m/z&amp;nbsp;scan range was set to 72 to 1000&amp;nbsp;m/z&amp;nbsp;under both positive and negative ionization mode. The automatic gain control (AGC) target was set to 3e6, and the maximum injection time (IT) was 200 ms. The resolution was set to 70,000.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Parallel reaction monitoring MS:&lt;/strong&gt; The MS2&amp;nbsp;spectra generation was performed on a Thermo Fisher Scientific Q Exactive Plus with a HESI source, which was set to a spray voltage of −2.7 kV under negative mode and 3.5 kV under positive mode. The sheath, auxiliary, and sweep gas flow rates were 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and aux gas heater was 360°C. The S-lens RF level was 45. The&amp;nbsp;m/z&amp;nbsp;scan ranges were specified for the four dipeptides and monitored for the full 22-min run time. The AGC target was set to 2 × 105, and the maximum IT was 100 ms. The resolution was set to 17,500. The isolation window was set to 2.0&amp;nbsp;m/z. Collision energy was set to a stepwise 30, 50, and 80 normalized collision energy (NCE). Our results suggest that a single MS2&amp;nbsp;spectrum may contain irrelevant&amp;nbsp;m/z&amp;nbsp;signals and the pseudospectrum, generated by correlating multiple MS2&amp;nbsp;spectra to the MS1-extracted ion chromatogram, is an effective approach to “clean” the MS2&amp;nbsp;spectrum. The pseudospectra were generated using a modified version of COVINA (Su et al. 2020).&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Synthesis of standards:&lt;/strong&gt; The dipeptide and tripeptide standards were synthesized and purified to 95% purity by GenScript USA (Piscataway, NJ). All standards were shipped at 25°C and stored at −20°C until they were prepared for analysis on the mass spectrometer. Parallel reaction monitoring was used to generate the MS2&amp;nbsp;spectra for comparison with samples. A total of 12 pmol of each standard was used in the analysis.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Metabolite Data analysis:&lt;/strong&gt; The MS1&amp;nbsp;data were processed using Maven (Melamud et al. 2010). The compound annotation was based on accurate mass and retention time match to the metabolite standards from the in-house library. The feature detection for untargeted metabolomics was done using Compound Discoverer (Thermo Fisher Scientific, version 3.1). Before the groupwise comparison, the signal intensities were normalized to the sample weight. Significance was determined by the groupwise signal intensity comparison of each metabolite at each time point using a Student’s&amp;nbsp;t&amp;nbsp;test that assumed unequal variance.&amp;nbsp;P&amp;nbsp;values were then adjusted using the Benjamini-Hochberg correction (false discovery rate, &amp;lt;0.05). Of those features with adjusted&amp;nbsp;P&amp;nbsp;&amp;lt; 0.05, the Fisher’s exact test was then applied to determine whether the putative dipeptides were significantly enriched in those features.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolite&amp;nbsp;&amp;nbsp;Data quality:&lt;/strong&gt; Before running the samples, the LC-MS system was evaluated for performance readiness by running commercially available standard mixtures and in-house standard mixtures to assess the mass accuracy, signal intensities, and the consistency of retention time. All known metabolites in the mixture were detected within 5 parts per million mass accuracy. Process blanks matching the composition of the extraction solvent were used in every sample batch to assess background signals and ensure that there was no carryover from one run to the next. In addition, the sample queue was randomized with respect to species and treatment to eliminate the potential for batch effects.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact PI Supplied Instrument Name: Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids. 2-ml glass Dounce homogenizer 2-ml glass Dounce homogenizer PI Supplied Instrument Name: 2-ml glass Dounce homogenizer Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Liquid chromatography mass spectrometer Liquid chromatography mass spectrometer PI Supplied Instrument Name: Liquid chromatography mass spectrometer Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ Thermo Fisher Scientific Q Exactive Plus Thermo Fisher Scientific Q Exactive Plus PI Supplied Instrument Name: Thermo Fisher Scientific Q Exactive Plus Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ anquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) anquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) PI Supplied Instrument Name: anquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.