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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;This dataset represents &amp;lt;em&amp;gt;Crassostrea virginica &amp;lt;/em&amp;gt;shell samples analyzed for trace and minor elements in adult Eastern oyster ocean acidification exposure experiments at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The collection and culturing of &amp;lt;em&amp;gt;C. virginica&amp;lt;/em&amp;gt; specimens are detailed in Downey-Wall, A.M., L.P. Cameron, B.M. Ford, E.M. McNally, Y.R. Venkataraman, S.B. Roberts, J.B. Ries, and K.E. Lotterhos. 2020. Ocean acidification induces subtle shifts in gene expression and DNA methylation in the mantle tissue of the Eastern oyster (&amp;lt;em&amp;gt;Crassostrea virginica&amp;lt;/em&amp;gt;). Frontiers in Marine Science doi: 10.3389/fmars.2020.566419.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Shells were cleaned thoroughly in 90 percent ethanol (Fisher Reagent Alcohol CAS: 64-17-5). Cleaned shells were dried at room temperature for 48 hours and stored in sealed plastic bags. The inner (lamellar) layer of oyster shells was sampled for elemental analysis. Shells were sampled by gently moving a Shiyang-III dental drill outfitted with a round bit across the low-Mg calcite surfaces of the interior shell. The powdered shell was placed in 15-milliliter (mL) polypropylene centrifuge tubes leached in 5 percent ultra-pure nitric acid solution (Fisher TraceMetal Grade Nitric Acid UN2031).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Elemental analysis&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Shell samples were analyzed for trace and minor elements by inductively coupled plasma mass spectrometry (ICPMS). Shell samples were also acidified with ultra-pure nitric acid for analysis. Shell samples were analyzed for a suite of 57 elements (including Ca) by ActLabs, Ontario, Canada using the ActLabs ICPMS Ultratrace 4 method.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/666497.rdf" xlink:title="OCE-1635423" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635423 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635423</gmx:Anchor>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/666496.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;NSF Award Abstract:&lt;/em&gt;&lt;br /&gt;
Marine ecosystems worldwide are threatened by ocean acidification, a process caused by the unprecedented rate at which carbon dioxide is increasing in the atmosphere. Since ocean change is predicted to be rapid, extreme, and widespread, marine species may face an &quot;adapt-or-die&quot; scenario. However, modifications to the DNA sequence may be induced in response to a stress like ocean acidification and then inherited. Such &quot;epigenetic&quot; modifications may hold the key to population viability under global climate change, but they have been understudied. The aim of this research is to characterize the role of DNA methylation, a heritable epigenetic system, in the response of Eastern oysters (Crassostrea virginica) to ocean acidification. The intellectual merit lies in the integrative approach, which will characterize the role of DNA methylation in the intergenerational response of oysters to ocean acidification. These interdisciplinary data, spanning from molecular to organismal levels, will provide insight into mechanisms that underlie the capacity of marine invertebrates to respond to ocean acidification and lay the foundation for future transgenerational studies. Ocean acidification currently threatens marine species worldwide and has already caused significant losses in aquaculture, especially in Crassostrea species. This research has broader impacts for breeding, aquaculture, and the economy. Under the investigators' &quot;Epigenetics to Ocean&quot; (E2O) training program, the investigators will build STEM talent in bioinformatics and biogeochemistry, expose girls in low-income school districts to careers in genomics, and advance the field through open science and reproducibility.&lt;/p&gt;
&lt;p&gt;This research will specifically test if intermittent exposure to low pH induces a methylation response with downstream beneficial effects for biomineralization. These methylation states could be inherited and confer a fitness advantage to larvae that possess them. Phase 1 of the project will use an exposure experiment to determine the degree to which DNA methylation is altered and regulates the response to OA. Data from this experiment will be used to test the hypotheses that (i) DNA methylation, induced in the tissue of shell formation (i.e., mantle tissue), is correlated with changes in transcription and regulation of pallial fluid pH (calcifying fluid pH, measured by microelectrode), and (ii) that methylation changes induced in the mantle tissue are also induced in the germline --indicating that such changes are potentially heritable. Phase 2 of the project will use a pair-mated cross experiment to test the hypothesis that parental exposure to OA alters larval traits (calcification rate, shell structure, and polymorph mineralogy). Larvae will be generated from parents exposed to OA or control seawater, and then raised under control or OA conditions. Results will be used to (i) characterize inheritance of induced methylation states, (ii) estimate the variance in larval traits explained by genotype, non-genetic maternal/paternal effects, adult OA exposure, larval OA exposure, and parental methylome, and (iii) test the hypothesis that adult exposure alters the heritability (a quantity that predicts evolutionary response) of larval traits. Since the effects of epigenetic phenomena on estimates of heritability are highly debated, the results would advance understanding of this important issue. Because the investigators could discover that DNA methylation is a mechanism for heritable plastic responses to OA, knowledge of this mechanism would significantly improve and potentially transform predictive models for how organisms respond to global change.&lt;/p&gt;</gco:CharacterString>
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            <gco:CharacterString>BCO-DMO catalogue of parameters from Shell Concentrations from an adult Eastern oyster ocean acidification exposure experiment on adult Eastern oysters from Plum Island Sound in 2017</gco:CharacterString>
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	Name: RunID
	Units: unitless
	Description: &lt;p&gt;Unique id for analysis run&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889314.rdf
	Name: SampleID
	Units: unitless
	Description: &lt;p&gt;Unique identifier for the sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889315.rdf
	Name: OystID
	Units: unitless
	Description: &lt;p&gt;Unique identifier for the bivalve used when sample ID was just labelled as shell&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889316.rdf
	Name: Species
	Units: unitless
	Description: &lt;p&gt;sample shell type; M = mantle R = repair AM = above mud UM = under mud&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889317.rdf
	Name: SampleType
	Units: unitless
	Description: &lt;p&gt;species that the shell sample was taken from&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889318.rdf
	Name: Ag_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of silver&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889319.rdf
	Name: Al_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of aluminum&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889320.rdf
	Name: As_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of arsenic&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889321.rdf
	Name: Au_ppb
	Units: parts per billion
	Description: &lt;p&gt;measured concentration of gold&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889322.rdf
	Name: B_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of boron&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889323.rdf
	Name: Ba_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of barium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889324.rdf
	Name: Be_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of beryllium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889325.rdf
	Name: Bi_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of bismuth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889326.rdf
	Name: Ca_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of calcium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889327.rdf
	Name: Cd_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of cadmium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889328.rdf
	Name: Ce_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of Cerium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889329.rdf
	Name: Co_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of cobalt&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889330.rdf
	Name: Cr_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of chromium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889331.rdf
	Name: Cs_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of cesium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889332.rdf
	Name: Cu_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of copper&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889333.rdf
	Name: Dy_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of dysprosium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889334.rdf
	Name: Er_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of erbium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889335.rdf
	Name: Eu_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of europium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889336.rdf
	Name: Fe_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of iron&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889337.rdf
	Name: Ga_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of gallium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889338.rdf
	Name: Gd_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of gadolinium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889339.rdf
	Name: Ge_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of germanium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889340.rdf
	Name: Hf_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of hafnium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889341.rdf
	Name: Hg_ppb
	Units: parts per billion
	Description: &lt;p&gt;measured concentration of mercury&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889342.rdf
	Name: Ho_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of holmium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889343.rdf
	Name: In_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of indium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889344.rdf
	Name: K_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of potassium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889345.rdf
	Name: La_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of lanthanum&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889346.rdf
	Name: Li_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of lithium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889347.rdf
	Name: Lu_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of lutetium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889348.rdf
	Name: Mg_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of magnesium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889349.rdf
	Name: Mn_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of manganese&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889350.rdf
	Name: Mo_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of molybdenum&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889351.rdf
	Name: Na_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of sodium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889352.rdf
	Name: Nb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of niobium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889353.rdf
	Name: Nd_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of neodymium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889354.rdf
	Name: Ni_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of nickel&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889355.rdf
	Name: P_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of phosphorus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889356.rdf
	Name: Pb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of lead&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889357.rdf
	Name: Pr_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of praseodymium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889358.rdf
	Name: Rb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of rubidium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889359.rdf
	Name: Re_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of rhenium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889360.rdf
	Name: S_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of sulfur&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889361.rdf
	Name: Sb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of antimony&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889362.rdf
	Name: Sc_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of scandium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889363.rdf
	Name: Se_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of selenium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889364.rdf
	Name: Sm_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of samarium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889365.rdf
	Name: Sn_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of tin&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889366.rdf
	Name: Sr_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of strontium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889367.rdf
	Name: Ta_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of tantalum&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889368.rdf
	Name: Tb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of terbium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889369.rdf
	Name: Te_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of tellurium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889370.rdf
	Name: Th_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of thorium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889371.rdf
	Name: Ti_perc
	Units: percent
	Description: &lt;p&gt;measured concentration of titanium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889372.rdf
	Name: Tl_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of thallium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889373.rdf
	Name: Tm_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of thulium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889374.rdf
	Name: U_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of uranium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889375.rdf
	Name: V_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of vanadium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889376.rdf
	Name: W_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of tungsten&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889377.rdf
	Name: Y_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of yttrium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889378.rdf
	Name: Yb_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of ytterbium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889379.rdf
	Name: Zn_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of zinc&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/889380.rdf
	Name: Zr_ppm
	Units: parts per million
	Description: &lt;p&gt;measured concentration of zirconium&lt;/p&gt; 
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&amp;lt;p&amp;gt;The collection and culturing of &amp;lt;em&amp;gt;C. virginica&amp;lt;/em&amp;gt; specimens are detailed in Downey-Wall, A.M., L.P. Cameron, B.M. Ford, E.M. McNally, Y.R. Venkataraman, S.B. Roberts, J.B. Ries, and K.E. Lotterhos. 2020. Ocean acidification induces subtle shifts in gene expression and DNA methylation in the mantle tissue of the Eastern oyster (&amp;lt;em&amp;gt;Crassostrea virginica&amp;lt;/em&amp;gt;). Frontiers in Marine Science doi: 10.3389/fmars.2020.566419.&amp;lt;/p&amp;gt;

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