http://lod.bco-dmo.org/id/dataset/888911
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-02-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Molar Ratios from an adult Eastern oyster ocean acidification exposure experiment at the Northeastern University Marine Science Center in 2017
2023-02-02
publication
2023-02-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-02-23
publication
https://doi.org/10.26008/1912/bco-dmo.888911.1
Katie Lotterhos
Northeastern University
principalInvestigator
Justin B. Ries
Northeastern University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lotterhos, K., Ries, J. B. (2023) Molar Ratios from an adult Eastern oyster ocean acidification exposure experiment at the Northeastern University Marine Science Center in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.888911.1 [access date]
Methods and Sampling: <p>In this study, we examined the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (<em>Crassostrea virginica</em>) exposed to experimental ocean acidification (OA) over 80 days. The collection and culturing of <em>C. virginica</em> specimens are detailed in Downey-Wall, A.M., L.P. Cameron, B.M. Ford, E.M. McNally, Y.R. Venkataraman, S.B. Roberts, J.B. Ries, and K.E. Lotterhos. 2020. Ocean acidification induces subtle shifts in gene expression and DNA methylation in the mantle tissue of the Eastern oyster (<em>Crassostrea virginica</em>). Frontiers in Marine Science doi: 10.3389/fmars.2020.566419.</p>
<p><strong>Elemental analysis</strong><br />
Extrapallial fluid, seawater, and shell samples were analyzed for trace and minor elements by inductively coupled plasma mass spectrometry (ICPMS). Liquid samples (i.e., EPF, seawater) were diluted to less than 0.05 percent total dissolved solid content with ultra-pure deionized water in 15 mL polypropylene centrifuge tubes leached in the same manner as those for shell samples. The samples were acidified with ultra-pure nitric acid (Fisher TraceMetal Grade Nitric Acid UN2031). Shell samples were also acidified with ultra-pure nitric acid for analysis.</p>
<p>Extrapallial fluid, seawater, and shell samples were analyzed for a suite of 57 elements (including Ca) by ActLabs, Ontario, Canada. In addition to the common suite of elements, liquid samples were analyzed for Si and shell samples were analyzed for S, P, Au, B, and Re. Liquid samples were analyzed using the ActLabs ICPMS method. Shell samples were analyzed using the ActLabs ICPMS Ultratrace 4 method:<br />
<a href="https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Factlabs.com%2Fgeochemistry%2Fexploration-geochemistry%2F4-acid-near-total-digestion%2F&amp;data=05%7C01%7Ctheyl%40whoi.edu%7Cd197caf6501c4ad19e3c08db11da102a%7Cd44c5cc6d18c46cc8abd4fdf5b6e5944%7C0%7C0%7C638123400510250507%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&amp;sdata=8xiYuBblGcRuHksZ3ef11uQxUAysIuSoTdC6U3wC%2BSQ%3D&amp;reserved=0">https://actlabs.com/geochemistry/exploration-geochemistry/4-acid-near-total-digestion/</a></p>
<p>This dataset represents molar ratio data that were processed using R (v. 4.0.3; R Core Team 2020) using the graphical interface RStudio (v. 1.0.1073) and then converted to molar ratios to calcium.&nbsp; For each sample type (EPF, seawater, shell), values were identified as outliers if the E/Ca ratio deviated by more than 103 from the mean E/Ca ratio for that element. This approach to identifying outliers removes those caused by sampling or equipment error but is more conservative than Tukey's method to identify and remove outliers.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635423 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635423
completed
Katie Lotterhos
Northeastern University
781-581-7370 X304
Northeastern University Marine Sciences Center 430 Nahant Rd.
Nahant
MA
01908
USA
k.lotterhos@northeastern.edu
pointOfContact
Justin B. Ries
Northeastern University
781-581-7370
Northeastern University Marine Science Center 430 Nahant Rd.
Nahant
MA
01908
USA
j.ries@northeastern.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
SampleType
Species
SampleID
TankID
ShellType
Ag_Ca
Al_Ca
As_Ca
Au_Ca
B_Ca
Ba_Ca
Be_Ca
Bi_Ca
Ca_Ca
Cd_Ca
Ce_Ca
Co_Ca
Cr_Ca
Cs_Ca
Cu_Ca
Dy_Ca
Er_Ca
Eu_Ca
Fe_Ca
Ga_Ca
Gd_Ca
Ge_Ca
Hf_Ca
Hg_Ca
Ho_Ca
In_Ca
K_Ca
La_Ca
Li_Ca
Lu_Ca
Mg_Ca
Mn_Ca
Mo_Ca
Na_Ca
Nb_Ca
Nd_Ca
Ni_Ca
P_Ca
Pb_Ca
Pr_Ca
Rb_Ca
Re_Ca
S_Ca
Sb_Ca
Sc_Ca
Se_Ca
Si_Ca
Sm_Ca
Sn_Ca
Sr_Ca
Ta_Ca
Tb_Ca
Te_Ca
Th_Ca
Ti_Ca
Tl_Ca
Tm_Ca
U_Ca
V_Ca
W_Ca
Y_Ca
Yb_Ca
Zn_Ca
Zr_Ca
Shiyang-III dental drill
Mettler Toledo scale
theme
None, User defined
sample type
species scientific name (binomial)
sample identification
tank
ratio
featureType
BCO-DMO Standard Parameters
Drill
Inductively Coupled Plasma Mass Spectrometer
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?
https://osprey.bco-dmo.org/project/666496
Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?
<p><em>NSF Award Abstract:</em><br />
Marine ecosystems worldwide are threatened by ocean acidification, a process caused by the unprecedented rate at which carbon dioxide is increasing in the atmosphere. Since ocean change is predicted to be rapid, extreme, and widespread, marine species may face an "adapt-or-die" scenario. However, modifications to the DNA sequence may be induced in response to a stress like ocean acidification and then inherited. Such "epigenetic" modifications may hold the key to population viability under global climate change, but they have been understudied. The aim of this research is to characterize the role of DNA methylation, a heritable epigenetic system, in the response of Eastern oysters (Crassostrea virginica) to ocean acidification. The intellectual merit lies in the integrative approach, which will characterize the role of DNA methylation in the intergenerational response of oysters to ocean acidification. These interdisciplinary data, spanning from molecular to organismal levels, will provide insight into mechanisms that underlie the capacity of marine invertebrates to respond to ocean acidification and lay the foundation for future transgenerational studies. Ocean acidification currently threatens marine species worldwide and has already caused significant losses in aquaculture, especially in Crassostrea species. This research has broader impacts for breeding, aquaculture, and the economy. Under the investigators' "Epigenetics to Ocean" (E2O) training program, the investigators will build STEM talent in bioinformatics and biogeochemistry, expose girls in low-income school districts to careers in genomics, and advance the field through open science and reproducibility.</p>
<p>This research will specifically test if intermittent exposure to low pH induces a methylation response with downstream beneficial effects for biomineralization. These methylation states could be inherited and confer a fitness advantage to larvae that possess them. Phase 1 of the project will use an exposure experiment to determine the degree to which DNA methylation is altered and regulates the response to OA. Data from this experiment will be used to test the hypotheses that (i) DNA methylation, induced in the tissue of shell formation (i.e., mantle tissue), is correlated with changes in transcription and regulation of pallial fluid pH (calcifying fluid pH, measured by microelectrode), and (ii) that methylation changes induced in the mantle tissue are also induced in the germline --indicating that such changes are potentially heritable. Phase 2 of the project will use a pair-mated cross experiment to test the hypothesis that parental exposure to OA alters larval traits (calcification rate, shell structure, and polymorph mineralogy). Larvae will be generated from parents exposed to OA or control seawater, and then raised under control or OA conditions. Results will be used to (i) characterize inheritance of induced methylation states, (ii) estimate the variance in larval traits explained by genotype, non-genetic maternal/paternal effects, adult OA exposure, larval OA exposure, and parental methylome, and (iii) test the hypothesis that adult exposure alters the heritability (a quantity that predicts evolutionary response) of larval traits. Since the effects of epigenetic phenomena on estimates of heritability are highly debated, the results would advance understanding of this important issue. Because the investigators could discover that DNA methylation is a mechanism for heritable plastic responses to OA, knowledge of this mechanism would significantly improve and potentially transform predictive models for how organisms respond to global change.</p>
Epigenetics to Ocean
largerWorkCitation
project
eng; USA
biota
oceans
-70.837023
-70.837023
42.751636
42.751636
2017-04-01
2017-04-01
Coastal Massachusetts near Nahant: 42°25'06"N 70°54'14"W
0
BCO-DMO catalogue of parameters from Molar Ratios from an adult Eastern oyster ocean acidification exposure experiment at the Northeastern University Marine Science Center in 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/889381.rdf
Name: SampleType
Units: unitless
Description: <p>type of sample EPF= extrapallial fluid, seawater, or shell</p>
http://lod.bco-dmo.org/id/dataset-parameter/889382.rdf
Name: Species
Units: unitless
Description: <p>Species that the sample was taken from. C_virginica is Crassostrea virginica</p>
http://lod.bco-dmo.org/id/dataset-parameter/889383.rdf
Name: SampleID
Units: unitless
Description: <p>Unique identifier for the sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/889384.rdf
Name: TankID
Units: unitless
Description: <p>Unique identifier for the tank</p>
http://lod.bco-dmo.org/id/dataset-parameter/889385.rdf
Name: ShellType
Units: unitless
Description: <p>sample shell type; M = mantle R = repair AM = above mud UM = under mud</p>
http://lod.bco-dmo.org/id/dataset-parameter/889386.rdf
Name: Ag_Ca
Units: unitless
Description: <p>molar ratio of silver to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889387.rdf
Name: Al_Ca
Units: unitless
Description: <p>molar ratio of aluminum to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889388.rdf
Name: As_Ca
Units: unitless
Description: <p>molar ratio of arsenic to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889389.rdf
Name: Au_Ca
Units: unitless
Description: <p>molar ratio of gold to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889390.rdf
Name: B_Ca
Units: unitless
Description: <p>molar ratio of boron to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889391.rdf
Name: Ba_Ca
Units: unitless
Description: <p>molar ratio of barium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889392.rdf
Name: Be_Ca
Units: unitless
Description: <p>molar ratio of beryllium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889393.rdf
Name: Bi_Ca
Units: unitless
Description: <p>molar ratio of bismuth to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889394.rdf
Name: Ca_Ca
Units: unitless
Description: <p>molar ratio of calcium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889395.rdf
Name: Cd_Ca
Units: unitless
Description: <p>molar ratio of cadmium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889396.rdf
Name: Ce_Ca
Units: unitless
Description: <p>molar ratio of cerium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889397.rdf
Name: Co_Ca
Units: unitless
Description: <p>molar ratio of cobalt to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889398.rdf
Name: Cr_Ca
Units: unitless
Description: <p>molar ratio of chromium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889399.rdf
Name: Cs_Ca
Units: unitless
Description: <p>molar ratio of cesium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889400.rdf
Name: Cu_Ca
Units: unitless
Description: <p>molar ratio of copper to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889401.rdf
Name: Dy_Ca
Units: unitless
Description: <p>molar ratio of dysrosium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889402.rdf
Name: Er_Ca
Units: unitless
Description: <p>molar ratio of erbium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889403.rdf
Name: Eu_Ca
Units: unitless
Description: <p>molar ratio of europium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889404.rdf
Name: Fe_Ca
Units: unitless
Description: <p>molar ratio of iron to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889405.rdf
Name: Ga_Ca
Units: unitless
Description: <p>molar ratio of gallium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889406.rdf
Name: Gd_Ca
Units: unitless
Description: <p>molar ration of gadolinium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889407.rdf
Name: Ge_Ca
Units: unitless
Description: <p>molar ratio of germanium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889408.rdf
Name: Hf_Ca
Units: unitless
Description: <p>molar ratio of hafnium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889409.rdf
Name: Hg_Ca
Units: unitless
Description: <p>molar ratio of mercury to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889410.rdf
Name: Ho_Ca
Units: unitless
Description: <p>molar ratio of holmium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889411.rdf
Name: In_Ca
Units: unitless
Description: <p>molar ratio of indium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889412.rdf
Name: K_Ca
Units: unitless
Description: <p>molar ratio of potassium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889413.rdf
Name: La_Ca
Units: unitless
Description: <p>molar ratio of lanthanum to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889414.rdf
Name: Li_Ca
Units: unitless
Description: <p>molar ratio of lithium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889415.rdf
Name: Lu_Ca
Units: unitless
Description: <p>molar ratio of lutetium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889416.rdf
Name: Mg_Ca
Units: unitless
Description: <p>molar ratio of magnesium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889417.rdf
Name: Mn_Ca
Units: unitless
Description: <p>molar ratio of manganese to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889418.rdf
Name: Mo_Ca
Units: unitless
Description: <p>molar ratio of molybdenum to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889419.rdf
Name: Na_Ca
Units: unitless
Description: <p>molar ratio of sodium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889420.rdf
Name: Nb_Ca
Units: unitless
Description: <p>molar ratio of niobium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889421.rdf
Name: Nd_Ca
Units: unitless
Description: <p>molar ratio of neodymium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889422.rdf
Name: Ni_Ca
Units: unitless
Description: <p>molar ratio of nickel to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889423.rdf
Name: P_Ca
Units: unitless
Description: <p>molar ratio of phosphorous to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889424.rdf
Name: Pb_Ca
Units: unitless
Description: <p>molar ratio of lead to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889425.rdf
Name: Pr_Ca
Units: unitless
Description: <p>molar ratio of praseodymium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889426.rdf
Name: Rb_Ca
Units: unitless
Description: <p>molar ratio of rubidium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889427.rdf
Name: Re_Ca
Units: unitless
Description: <p>molar ratio of rhenium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889428.rdf
Name: S_Ca
Units: unitless
Description: <p>molar ratio of sulfur to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889429.rdf
Name: Sb_Ca
Units: unitless
Description: <p>molar ratio of antimony to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889430.rdf
Name: Sc_Ca
Units: unitless
Description: <p>molar ratio of scandium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889431.rdf
Name: Se_Ca
Units: unitless
Description: <p>molar ratio of selenium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889432.rdf
Name: Si_Ca
Units: unitless
Description: <p>molar ratio of silicon to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889433.rdf
Name: Sm_Ca
Units: unitless
Description: <p>molar ratio of samarium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889434.rdf
Name: Sn_Ca
Units: unitless
Description: <p>molar ratio of tin to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889435.rdf
Name: Sr_Ca
Units: unitless
Description: <p>molar ratio of strontium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889436.rdf
Name: Ta_Ca
Units: unitless
Description: <p>molar ratio of tantalum to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889437.rdf
Name: Tb_Ca
Units: unitless
Description: <p>molar ratio of terbium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889438.rdf
Name: Te_Ca
Units: unitless
Description: <p>molar ratio of thorium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889439.rdf
Name: Th_Ca
Units: unitless
Description: <p>molar ratio of titanium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889440.rdf
Name: Ti_Ca
Units: unitless
Description: <p>molar ratio of thulium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889441.rdf
Name: Tl_Ca
Units: unitless
Description: <p>molar ratio of vanadium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889442.rdf
Name: Tm_Ca
Units: unitless
Description: <p>molar ratio of tungsten to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889443.rdf
Name: U_Ca
Units: unitless
Description: <p>molar ratio of uranium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889444.rdf
Name: V_Ca
Units: unitless
Description: <p>molar ratio of tellerium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889445.rdf
Name: W_Ca
Units: unitless
Description: <p>molar ratio of thallium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889446.rdf
Name: Y_Ca
Units: unitless
Description: <p>molar ratio of yttrium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889447.rdf
Name: Yb_Ca
Units: unitless
Description: <p>molar ratio of ytterbium to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889448.rdf
Name: Zn_Ca
Units: unitless
Description: <p>molar ratio of zinc to calcium</p>
http://lod.bco-dmo.org/id/dataset-parameter/889449.rdf
Name: Zr_Ca
Units: unitless
Description: <p>molar ratio of zirconium to calcium</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
102018
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/a38ab899-f629-59f3-ba76-9fb1ea6bc072/content
download
https://doi.org/10.26008/1912/bco-dmo.888911.1
download
onLine
dataset
<p>In this study, we examined the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (<em>Crassostrea virginica</em>) exposed to experimental ocean acidification (OA) over 80 days. The collection and culturing of <em>C. virginica</em> specimens are detailed in Downey-Wall, A.M., L.P. Cameron, B.M. Ford, E.M. McNally, Y.R. Venkataraman, S.B. Roberts, J.B. Ries, and K.E. Lotterhos. 2020. Ocean acidification induces subtle shifts in gene expression and DNA methylation in the mantle tissue of the Eastern oyster (<em>Crassostrea virginica</em>). Frontiers in Marine Science doi: 10.3389/fmars.2020.566419.</p>
<p><strong>Elemental analysis</strong><br />
Extrapallial fluid, seawater, and shell samples were analyzed for trace and minor elements by inductively coupled plasma mass spectrometry (ICPMS). Liquid samples (i.e., EPF, seawater) were diluted to less than 0.05 percent total dissolved solid content with ultra-pure deionized water in 15 mL polypropylene centrifuge tubes leached in the same manner as those for shell samples. The samples were acidified with ultra-pure nitric acid (Fisher TraceMetal Grade Nitric Acid UN2031). Shell samples were also acidified with ultra-pure nitric acid for analysis.</p>
<p>Extrapallial fluid, seawater, and shell samples were analyzed for a suite of 57 elements (including Ca) by ActLabs, Ontario, Canada. In addition to the common suite of elements, liquid samples were analyzed for Si and shell samples were analyzed for S, P, Au, B, and Re. Liquid samples were analyzed using the ActLabs ICPMS method. Shell samples were analyzed using the ActLabs ICPMS Ultratrace 4 method:<br />
<a href="https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Factlabs.com%2Fgeochemistry%2Fexploration-geochemistry%2F4-acid-near-total-digestion%2F&amp;data=05%7C01%7Ctheyl%40whoi.edu%7Cd197caf6501c4ad19e3c08db11da102a%7Cd44c5cc6d18c46cc8abd4fdf5b6e5944%7C0%7C0%7C638123400510250507%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&amp;sdata=8xiYuBblGcRuHksZ3ef11uQxUAysIuSoTdC6U3wC%2BSQ%3D&amp;reserved=0">https://actlabs.com/geochemistry/exploration-geochemistry/4-acid-near-total-digestion/</a></p>
<p>This dataset represents molar ratio data that were processed using R (v. 4.0.3; R Core Team 2020) using the graphical interface RStudio (v. 1.0.1073) and then converted to molar ratios to calcium.&nbsp; For each sample type (EPF, seawater, shell), values were identified as outliers if the E/Ca ratio deviated by more than 103 from the mean E/Ca ratio for that element. This approach to identifying outliers removes those caused by sampling or equipment error but is more conservative than Tukey's method to identify and remove outliers.</p>
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Specified by the Principal Investigator(s)
<p>Molar ratio data were processed using R (v. 4.0.3; R Core Team 2020) and the graphical interface RStudio (v. 1.0.1073).</p>
Specified by the Principal Investigator(s)
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Shiyang-III dental drill
Shiyang-III dental drill
PI Supplied Instrument Name: Shiyang-III dental drill Instrument Name: Drill Instrument Short Name: Instrument Description: A drill is a tool used for making round holes or driving fasteners. There are many types of drills: some are powered manually, and others use electricity (electric drill) or compressed air as the motive power. Drills with a percussive action (hammer drills) are mostly used in hard materials such as masonry (brick, concrete, and stone) or rock. Some types of hand-held drills are also used to drive screws and other fasteners.
PI Supplied Instrument Name: Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Mettler Toledo scale
Mettler Toledo scale
PI Supplied Instrument Name: Mettler Toledo scale PI Supplied Instrument Description:Mettler Toledo scale (precision = 0.001g) Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/