http://lod.bco-dmo.org/id/dataset/890835 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-02-28 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) from coastal waters and estuaries in North and South America, including the Caribbean and Gulf of Mexico from 2006 to 2020 2023-02-28 publication 2023-02-28 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-03-02 publication https://doi.org/10.26008/1912/bco-dmo.890835.1 Eric Schott University of Maryland Center for Environmental Science principalInvestigator Louis V. Plough University of Maryland Center for Environmental Science principalInvestigator Mingli Zhao University of Maryland at Baltimore principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Schott, E., Plough, L. V., Zhao, M. (2023) Nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) from coastal waters and estuaries in North and South America, including the Caribbean and Gulf of Mexico from 2006 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.890835.1 [access date] Methods and Sampling: &lt;p&gt;Location:&amp;nbsp;&lt;br /&gt; Coastal waters and estuaries N. America and S. America, including the Caribbean and Gulf of Mexico.&amp;nbsp;Collections from trapping, dredging, and netting by commercial and recreational fishers, and scientific collections by resource managers.&lt;br /&gt; &lt;br /&gt; Methods &amp;amp; Sampling:&lt;br /&gt; Crabs were obtained by collaborating partners in the Caribbean and South America using mixed methods of baited traps and line, nets, and dredges.&lt;/p&gt; &lt;p&gt;DNA sequencing was conducted using both Sanger sequencing (Big Dye terminator) of PCR amplicons at the BioAnalytical Lab in IMET (https://www.umces.edu/baslab).&lt;/p&gt; &lt;p&gt;Illumina-based sequencing of full virus genome amplifications used Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).&lt;/p&gt; &lt;p&gt;Instruments:&lt;br /&gt; No lab-specific environmental equipment was used to record metadata at the time of crab collection.&lt;/p&gt; &lt;p&gt;For sequence analysis, equipment was 3130 XL Genetic Analyzer from Life Technologies at IMET and contracted&amp;nbsp; Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).&lt;br /&gt; &lt;br /&gt; Taxonomic identifiers:&lt;br /&gt; blue crabs, Callinectes sapidus, urn:lsid:marinespecies.org:taxname:107379&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658466 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658466 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658396 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658396 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658389 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658389 completed Eric Schott University of Maryland Center for Environmental Science 410-234-8827 701 E Pratt St Baltimore MD 21202-3183 USA schott@umces.edu pointOfContact Louis V. Plough University of Maryland Center for Environmental Science 410-221-8474 P.O. Box 775 2020 Horns Point Rd, Cambridge MD 21613 USA lplough@umces.edu pointOfContact Mingli Zhao University of Maryland at Baltimore zmingli1@umbc.edu pointOfContact asNeeded Dataset Version: 1 Unknown theme None, User defined Automated DNA Sequencer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative research: Variation in life history and connectivity as drivers of pathogen-host dynamics and genetic structure in a trans-hemispheric pathosystem https://osprey.bco-dmo.org/project/739621 Collaborative research: Variation in life history and connectivity as drivers of pathogen-host dynamics and genetic structure in a trans-hemispheric pathosystem <p>NSF Award Abstract:</p> <p>Marine invertebrates use an array of strategies to survive, move, and reproduce across diverse and dynamic environmental conditions. This project investigates the intersection of these strategies and how they facilitate the persistence of blue crabs and a pathogenic virus along the Atlantic coast of North and South America. The widespread distribution of this crab-virus system makes it useful for investigating host-pathogen interactions. Blue crabs can reduce their activity level and induce winter dormancy in colder climates, but it is unclear how this alters progression and transmission of the pathogen. Conversely, year-round growth and reproduction of tropical blue crabs may be offset by higher pathogen abundance and activity. This project will use a combination of field and laboratory studies to reveal how crab life history and pathogen dynamics interact and adapt at the extremes of their range. Genetic sequencing, crab movement tracking and oceanographic models will be used to understand how crab-disease dynamics vary across temperate and tropical latitudes. The blue crab is an ecologically and economically important species and knowledge generated in this project will help provide management guidance to support sustainable fisheries. Best practices to avoid and limit disease will be communicated to commercial and artisanal harvesters through partnerships and workshops. Local high school and undergraduate students from underrepresented groups will be engaged through a variety of formal and informal educational programs. Public outreach will be implemented through a museum partnership with the Shedd Aquarium and will include the training of a science communication intern.</p> <p>This collaborative project will combine empirical field and laboratory experiments, population genomics, and biophysical modeling to explore the consequences of latitude-driven changes in life history and oceanic connectivity on a trans-hemispheric pathosystem comprised of the blue crab, Callinectes sapidus, and the pathogenic virus, CsRV1. The virulence of the CsRV1 virus from tropical and temperate latitudes and the impact of overwintering will be studied by experimental virus challenges of crabs transplanted between high and low latitudes. The impact of infection and virulence on crab movement will be investigated in laboratory raceway experiments of healthy and infected crabs and in the field with acoustically tagged crabs deployed in temperate and tropical locations. Population genetic studies using thousands of genome-wide RAD sequencing markers for crabs and whole-genome sequencing for the virus will define genetic connectivity of crab and virus populations across their range, and will investigate the possible latitudinal, seascape, and life history-driven changes in blue crab and virus genomes. The two population genomic data sets are expected to provide different inferences and scales of connectivity because CsRV1 virus genotypes are transmitted only among post-larval crabs while blue crab genotypes also move by a potentially long-range dispersive larval stage. Finally, integrated biophysical models will be used to investigate the relative contributions of adult and larval dispersal on the population structure of the crab and the pathogen across a broad swath of habitat between New England and Argentina with a decade of simulations. An open-source Lagrangian stochastic model will estimate pelagic larval transport, and spatially explicit biased-correlated random walk models will estimate adult movement. Models will be informed by experimentally-derived movement and behavior data, as well as information on crab larval and adult behavior and overwintering duration available in the published literature. Under a series of scenarios in which crab behavior is affected by latitude and virus infection, statistical comparisons will be made between biophysical model-based predictions of connectivity and genetic estimates of connectivity. These analyses will advance our understanding of the physical, environmental, and biological factors that shape the dynamics of the blue crab CsRV1 pathosystem.</p> Blue Crab Connectivity largerWorkCitation project eng; USA oceans -71.0929 -54.2921 -34.6285 41.5118 2006-01-01 2020-01-01 Atlantic coast of north and south America from Massachusetts to Southern Brazil, Caribbean 0 BCO-DMO catalogue of parameters from Nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) from coastal waters and estuaries in North and South America, including the Caribbean and Gulf of Mexico from 2006 to 2020 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 34085 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/8d722ee4-e061-5181-af39-f312c19dd922/content download 444798 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/67e87687-95ae-52e7-a4c3-dcc0587bcbe4/content download https://doi.org/10.26008/1912/bco-dmo.890835.1 download onLine dataset &lt;p&gt;Location:&amp;nbsp;&lt;br /&gt; Coastal waters and estuaries N. America and S. America, including the Caribbean and Gulf of Mexico.&amp;nbsp;Collections from trapping, dredging, and netting by commercial and recreational fishers, and scientific collections by resource managers.&lt;br /&gt; &lt;br /&gt; Methods &amp;amp; Sampling:&lt;br /&gt; Crabs were obtained by collaborating partners in the Caribbean and South America using mixed methods of baited traps and line, nets, and dredges.&lt;/p&gt; &lt;p&gt;DNA sequencing was conducted using both Sanger sequencing (Big Dye terminator) of PCR amplicons at the BioAnalytical Lab in IMET (https://www.umces.edu/baslab).&lt;/p&gt; &lt;p&gt;Illumina-based sequencing of full virus genome amplifications used Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).&lt;/p&gt; &lt;p&gt;Instruments:&lt;br /&gt; No lab-specific environmental equipment was used to record metadata at the time of crab collection.&lt;/p&gt; &lt;p&gt;For sequence analysis, equipment was 3130 XL Genetic Analyzer from Life Technologies at IMET and contracted&amp;nbsp; Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).&lt;br /&gt; &lt;br /&gt; Taxonomic identifiers:&lt;br /&gt; blue crabs, Callinectes sapidus, urn:lsid:marinespecies.org:taxname:107379&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO dataset processing notes:&lt;br /&gt; * lat lons formatted as decimal degrees (W and S are&amp;nbsp;negative)&lt;br /&gt; * empty depth column removed&lt;br /&gt; * upon request from the submitter all &quot;host&quot; values filled in as&amp;nbsp;Callinectes sapidus.&amp;nbsp; And VA site coordinates were corrected&amp;nbsp;from 38.0214,-76.3524 to 37.1477,-76.1573&lt;/p&gt; &lt;p&gt;.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact PI Supplied Instrument Name: PI Supplied Instrument Description:Illumina MiSeq Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.