http://lod.bco-dmo.org/id/dataset/890979 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-03-03 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Biogeochemistry, metabolomics, and metagenomics of Florida's Coral Reef from sampling conducted over 15 days in June 2019 2023-03-08 publication 2023-03-08 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-03-08 publication https://doi.org/10.26008/1912/bco-dmo.890979.1 Amy Apprill Woods Hole Oceanographic Institution principalInvestigator Elizabeth Kujawinski Woods Hole Oceanographic Institution principalInvestigator Abigail Clark Mote Marine Laboratory principalInvestigator Laura Gray Woods Hole Oceanographic Institution principalInvestigator Melissa C. Kido Soule Woods Hole Oceanographic Institution principalInvestigator Krista Longnecker Woods Hole Oceanographic Institution principalInvestigator Erinn M. Muller Mote Marine Laboratory principalInvestigator Stuart Sandin University of California-San Diego Scripps principalInvestigator Chris Sullivan University of California-San Diego Scripps principalInvestigator Brian Zgliczynski University of California-San Diego Scripps principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Apprill, A., Kujawinski, E., Muller, E., Sandin, S., Weber, L., Clark, A., Kido Soule, M. C., Longnecker, K., Zgliczynski, B., Sullivan, C., Becker, C. C. (2023) Biogeochemistry, metabolomics, and metagenomics of Florida's Coral Reef from sampling conducted over 15 days in June 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.890979.1 [access date] Reef biogeochemistry, metabolomics, and metagenomics Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Study Area:&lt;/strong&gt; We sampled coral reef environments during a research cruise aboard the M/V Alucia between June 5 and June 19, 2019. During this time, we conducted surveys and sampled biogeochemical seawater parameters at 85 reefs across 8 zones in Florida's Coral Reef, from the North Key Largo/Biscayne Bay area, designated as Zone 1, to the Dry Tortugas National Park (Zone 8). We selected reefs based on input from the Florida Fish and Wildlife Conservation Commission and existing reefs in long-term monitoring programs (e.g., Coral Reef Evaluation and Monitoring Project, CREMP).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample Collection and Ship-board Processing:&lt;/strong&gt; We conducted diver-based surveys to evaluate the prevalence of stony coral tissue loss disease (SCTLD) at each of the 85 reefs. At each site, one diver performed a 30-minute roving diver survey to determine the richness of scleractinian species, the presence or absence of stony coral tissue loss disease, and the size of all observed coral colonies. The diver assigned coral colonies to four size classes, based on diameter/length: &amp;lt;10 centimeters (cm), 10-25cm, 25-50cm, and &amp;gt;50cm. The area in square meters (m2) surveyed by each diver was estimated to calculate the density of corals at each site.&lt;/p&gt; &lt;p&gt;We generated 100 m2 plots at each of the 85 reefs for benthic surveys via high-resolution 2D orthophotomosaics. We analyzed 45 of these reefs for benthic composition data after determining this would sufficiently cover the observed variability in the different reefs.&lt;/p&gt; &lt;p&gt;We collected discrete seawater samples at all 85 reefs to measure inorganic nutrient (phosphate, ammonium, silicate, nitrite plus nitrate) concentrations, total organic carbon (TOC) and total nitrogen (TN) concentrations, and cell abundances (heterotrophic microbes (unpigmented bacteria and archaea), &lt;em&gt;Prochlorococcus&lt;/em&gt;, &lt;em&gt;Synechococcus&lt;/em&gt;, and picoeukaryotes). We collected samples via SCUBA with acid-washed and combusted 40-milliliter (mL) borosilicate glass vials for TOC and TN collections and 30 mL acid-washed square bottles (HDPE, Nalgene, ThermoFisher Scientific, Waltham, MA, USA) for nutrient collections, and filled both vials while at reef depth. Samples were kept on ice in a cooler for less than 4 hours prior to processing. Once on board the M/V Alucia, we processed all samples. We added 75 microliters (μL) of phosphoric acid to the 40 mL glass vials to fix the samples for TOC and TN, and kept these samples at room temperature or 4°C until laboratory analysis. We removed 1.4 mL of seawater from the nutrient bottles, mixed it with 8% paraformaldehyde (1% final concentration, Electron Microscopy Sciences), fixed it in the dark for 20 minutes at 4°C, then froze it at -80°C. We capped the 30 mL inorganic nutrient bottles and placed them at -80°C until analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolomic Analyses:&lt;/strong&gt; We collected seawater for targeted and untargeted metabolomic analyses at 13 reefs across the 8 zones of Florida’s Coral Reef. At each reef, we collected seawater in 1.7L Niskin bottles via SCUBA at three distinct locations on the reef for biological replication. These Niskin bottles were kept in a cooler for less than 4 hours prior to processing on the M/V Alucia. Once back on board the M/V Alucia, we transferred seawater from the Niskin bottles into acid-washed 2L polycarbonate bottles using acid-washed PharMedBPT tubing (Masterflex, Cole-Parmer, Vernon Hills, IL, USA). These water samples were processed as described previously by Weber et al. (2020). Briefly, we prefiltered the seawater through a 47-millimeter (mm) 0.1-micrometer (μm) pore size polytetrafluoroethylene filter (Omnipore, EMD Millipore Corporation, Billerica, MA, USA) to remove all microbial biomass via peristalsis and placed the filtered seawater directly into a second acid-washed 2L polycarbonate bottle. We acidified this filtrate with 2 mL OPTIMA-grade 12 M (molar) hydrochloric acid prior to solid phase extraction (SPE). We used SPE to concentrate metabolites (primarily low molecular weight dissolved organic matter) from the filtered seawater. We used a Waters vacuum manifold to slowly pass the seawater through 1 gram per 6 cubic centimeters (g/cc) SPE cartridges (Bond Elut PPL; Agilent, Santa Clara, CA, United States) pre-conditioned with HPLC-grade methanol and weighed bottles with seawater prior to and following SPE to calculate the volume of seawater filtered. SPE cartridges were wrapped in combusted aluminum foil and frozen to -80°C prior to analysis at the Woods Hole Oceanographic Institution.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Microbial Biomass and Chlorophyll Analyses&lt;/strong&gt;: We collected seawater for microbial biomass and chlorophyll analysis at 27 reefs across the 8 zones of Florida’s Coral Reef. At each reef, we employed a groundwater pump (Mini-Monsoon 12V, Proactive Environmental Products, Bradenton, Florida, USA) to pump seawater from just above the reef benthos into acid-washed or 10% bleach-rinsed 4L LDPE bottles (Nalgene). Samples were kept in a cooler on ice until processing less than 4 hours following collection. Once back on board the M/V Alucia, we used peristalsis to filter 2L of seawater to obtain duplicates from each reef through a 0.2 μm Supor filter (Pall, Port Washington, New York, USA) for microbial biomass housed in a 25 mm filter holder (Swinnex-25, Millipore Corporation), as described previously (Becker et al. 2020). Chlorophyll samples were obtained by filtering 2L of seawater in duplicate with the same peristalsis setup, but using a GF/F filter. We placed filters (GF/F or 0.2 μm) into 2 mL cryovials and froze them at -80°C prior to further processing at the Woods Hole Oceanographic Institution.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral Tissue Analyses:&lt;/strong&gt; On reefs with active stony coral tissue loss disease, we collected coral tissue and near-coral seawater samples from apparently healthy and actively diseased coral colonies. We aimed to only sample reefs where at least three healthy coral colonies were present in addition to at least three diseased colonies. Sample collection proceeded on near-coral seawater followed by coral tissue as described previously (Becker et al. 2021). Prior to tissue collection, we collected near-coral seawater via a 60 mL syringe within 1-5 cm of the lesion margin on diseased corals or healthy tissue from apparently healthy colonies. Following near-coral seawater collections, we sampled tissue of diseased corals at the lesion margin between apparently healthy tissue and bleached and sloughing tissue or apparently healthy colonies (without any indication of disease or other affliction) randomly on the coral head. We collected tissue samples with Luer slip 10 mL syringes. Syringes were quickly placed into small Whirl-Pak bags to contain any mucus and tissue leaking from the syringe. Following the collections, all tissue and near-coral seawater syringe samples were placed on ice prior to processing. We transferred samples of coral tissue and mucus into 15 ml conical tubes and froze them at -80°C until analysis. We attached filter holders containing 25 mm 0.2 μm Supor filters to the 60 mL luer-lock syringes and depressed them by hand to capture microbial biomass on the filters. We placed filters into labeled 2 mL cryovials and placed them in a -80°C freezer until analysis.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736288 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736288 completed Amy Apprill Woods Hole Oceanographic Institution 508-289-2649 Marine Chemistry and Geochemistry Department 266 Woods Hole Road, MS# 4 Woods Hole MA 02543 USA aapprill@whoi.edu pointOfContact Elizabeth Kujawinski Woods Hole Oceanographic Institution 508-289-3493 266 Woods Hole Rd. MS #4 Woods Hole MA 02543 USA ekujawinski@whoi.edu pointOfContact Abigail Clark Mote Marine Laboratory aclark@mote.org pointOfContact Laura Gray Woods Hole Oceanographic Institution 508-289-2981; 301-602-1540 Marine Chemistry and Geochemistry Department 266 Woods Hole Road, Mailstop 4 Woods Hole MA 02543 USA laura.gray@whoi.edu pointOfContact Melissa C. Kido Soule Woods Hole Oceanographic Institution 508-289-2879 Marine Chemistry and Geochemistry, MS#4 Woods Hole MA 02543 USA msoule@whoi.edu pointOfContact Krista Longnecker Woods Hole Oceanographic Institution 508-289-2824 266 Woods Hole Road MS# 04 Woods Hole MA 02543 USA klongnecker@whoi.edu pointOfContact Erinn M. Muller Mote Marine Laboratory 941-388-4441 x 310 1600 Ken Thompson Parkway Sarasota FL 34241 USA emuller@mote.org pointOfContact Stuart Sandin University of California-San Diego Scripps 619-861-0830 8602 La Jolla Shores Dr La Jolla CA 920930210 US ssandin@ucsd.edu pointOfContact Chris Sullivan University of California-San Diego Scripps cjsulliv@ucsd.edu pointOfContact Brian Zgliczynski University of California-San Diego Scripps bzgliczy@ucsd.edu pointOfContact asNeeded Dataset Version: 1 Unknown sample_name analysis_type Project_Accession biosample_accession sample_type stationID collection_date depth geo_loc_name latitude longitude replicate depth_ft host ZoneNum FL_Region Collaborator_siteID Habitat prochlorococcus_cells_per_ml synechococcus_cells_per_ml picoeukaryotes_cells_per_ml unpigmented_cells_per_ml npoc_uM tn_uM phosphate_uM nitriteandnitrate_uM silicate_uM nitrite_uM ammonium_uM nitrate_uM Temperature_C Dissolved_Oxygen_at_depth_percent_saturation Dissolved_Oxygen_at_depth_mg_per_liter SCTLDprevalence Calcified_Macroalgae Crustose_Coralline_Algae Cyanobacteria Fleshy_Macroalgae Hard_coral Invertebrates Non_biological Other Other_invertebrates Seagrass Soft_coral Sponge Turf_Algae Chlorophyll_mg_per_l Phaeophytin_mg_per_l coral healthbinary libraryID sampleID coralspecies sworcoral healthlong Illumina MiSeq Illumina NextSeq 2000 Illumina iSeq 100 System Beckman-Coulter Altra flow cytometer Accela Open Autosampler Orbitrap Fusion Lumos Tribid mass spectrometer Niskin bottles groundwater pump TSQ Vantage, Thermo Fisher ScientificTM luer slip and luer lock syringes Technicon AutoAnalyzer II Shimadzu TOC-VCSH TOC analyzer AquaFluor fluorometer Vanquish UHPLC, Thermo ScientificTM Waters Vacuum Manifold (Agilent) theme None, User defined sample identification sample description accession number NCBI BioSample accession station date depth No BCO-DMO term latitude longitude replicate species scientific name (binomial) site site description abundance total organic Carbon Nitrogen phosphate nitrate plus nitrite Silicate, Si(OH)4, silicic acid Nitrite Ammonium Nitrate water temperature at measurement depth dissolved Oxygen chlorophyll a total phaeopigment taxon_code taxon featureType BCO-DMO Standard Parameters Automated DNA Sequencer Automated DNA Sequencer Automated DNA Sequencer Flow Cytometer Laboratory Autosampler Mass Spectrometer Niskin bottle Pump Quadrupole Mass Spectrometer syringe Technicon AutoAnalyzer II Total Organic Carbon Analyzer Turner Designs Aquafluor 8000 Ultra-high-performance liquid chromatography vacuum manifold instrument BCO-DMO Standard Instruments OceanX service Deployment Activity Miami, FL, USA to Dry Tortugas National Park place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Signature exometabolomes of Caribbean corals and influences on reef picoplankton https://osprey.bco-dmo.org/project/746196 Signature exometabolomes of Caribbean corals and influences on reef picoplankton <p><em>NSF Award Abstract:</em><br /> Coral reefs are some of the most diverse and productive ecosystems in the ocean. Globally, reefs have declined in stony (reef-building) coral abundance due to environmental variations, and in the Caribbean this decline has coincided with an increase in octocoral (soft coral) abundance. This phase shift occurring on Caribbean reefs may be impacting the interactions between the sea floor and water column and particularly between corals and picoplankton. Picoplankton are the microorganisms in the water column that utilize organic matter released from corals to support their growth. These coral-picoplankton interactions are relatively unstudied, but could have major implications for reef ecology and coral health. This project will take place in the U.S. territory of the Virgin Islands (USVI) and will produce the first detailed knowledge about the chemical diversity and composition of organic matter released from diverse stony coral and octocoral species. This project will advance our understanding of coral reef microbial ecology by allowing us to understand how different coral metabolites impact picoplankton growth and dynamics over time. The results from this project will be made publically accessible in a freely available online magazine, and USVI minority middle and high school students will be exposed to a lesson about chemical-biological interactions on coral reefs through established summer camps. This project will also contribute to the training of USVI minority undergraduates as well as a graduate student.</p> <p>Coral exometabolomes, which are the sum of metabolic products of the coral together with its microbiome, are thought to structure picoplankton communities in a species-specific manner. However, a detailed understanding of coral exometabolomes, and their influences on reef picoplankton, has not yet been obtained. This project will utilize controlled aquaria-based experiments with stony corals and octocorals, foundational species of Caribbean reef ecosystems, to examine how the exometabolomes of diverse coral species differentially influence the reef picoplankton community. Specifically, this project will capitalize on recent developments in mass spectrometry-based metabolomics to define the signature exometabolomes of ecologically important and diverse stony corals and octocorals. Secondly, this project will determine how the exometabolomes of these corals vary with factors linked to coral taxonomy as well as the coral-associated microbiome (Symbiodinium algae, bacteria and archaea). With this new understanding of coral exometabolomes, the project will then apply a stable isotope probe labeling approach to the coral exometabolome and will examine if and how (through changes in growth and activity) the seawater picoplankton community incorporates coral exometabolomes from different coral species over time. This project will advance our ability to evaluate the role that coral exometabolomes play in contributing to benthic-picoplankton interactions on changing Caribbean reefs.</p> Coral Exometabolomes largerWorkCitation project eng; USA oceans Miami, FL, USA to Dry Tortugas National Park -82.9496 -70.6479 24.2519 41.5334 2019-06-05 2019-06-19 U.S. Virgin Islands 0 BCO-DMO catalogue of parameters from Biogeochemistry, metabolomics, and metagenomics of Florida's Coral Reef from sampling conducted over 15 days in June 2019 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/891130.rdf Name: sample_name Units: unitless Description: <p>Generic name of a sample. Could be an individual water or tissue sample for sequencing or the collection of metadata from a site that was not used for sequencing.</p> http://lod.bco-dmo.org/id/dataset-parameter/891131.rdf Name: analysis_type Units: unitless Description: <p>Quick description of the type of sample included. One of: metabolome, environmental metadata, 16S rRNA microbiome, or shotgun metagenome</p> http://lod.bco-dmo.org/id/dataset-parameter/891132.rdf Name: Project_Accession Units: unitless Description: <p>Project accession number for either the MetaboLights database or NCBI Sequence Read Archive. For the environmental metadata, this is not relevant. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891133.rdf Name: biosample_accession Units: unitless Description: <p>Accession number for a specific sample. Only relevant for the sequencing data uploaded to the NCBI Sequence Read Archive. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891134.rdf Name: sample_type Units: unitless Description: <p>Sample type is one of, seawater metabolome, environmental metadata, seawater metagenome, or coral metagenome</p> http://lod.bco-dmo.org/id/dataset-parameter/891135.rdf Name: stationID Units: unitless Description: <p>Generic name of the reef site used for the study. Not applicable is used for any laboratory-added control samples not collected at a reef. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891136.rdf Name: collection_date Units: unitless Description: <p>Date of collection, typically in format YYYY-MM-DD; years of 2020 or 2021 indicate controls that were introduced into the analysis during laboratory processing at Woods Hole Oceanographic Institution (WHOI).</p> http://lod.bco-dmo.org/id/dataset-parameter/891137.rdf Name: depth Units: meters Description: <p>Depth of reef. "Not applicable" = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891138.rdf Name: geo_loc_name Units: unitless Description: <p>Broad location of the collection (Country: State); samples with "Massachusetts" in this column are controls that were introduced into the analysis during laboratory processing at Woods Hole Oceanographic Institution (WHOI).</p> http://lod.bco-dmo.org/id/dataset-parameter/891139.rdf Name: latitude Units: decimal degrees Description: <p>Station latitude, south is negative. "Not applicable" = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891140.rdf Name: longitude Units: decimal degrees Description: <p>Station longitude, west is negative. "Not applicable" = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891141.rdf Name: replicate Units: unitless Description: <p>Replicate if there is duplicate metadata for a sample</p> http://lod.bco-dmo.org/id/dataset-parameter/891142.rdf Name: depth_ft Units: feet Description: <p>Depth of reef. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891143.rdf Name: host Units: unitless Description: <p>Species of coral host of microbiome sample, only relevant for coral metagenome samples and near-coral seawater samples. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891144.rdf Name: ZoneNum Units: unitless Description: <p>Zone of Florida&#039;s Coral Reef sampled. One of eight zones. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891145.rdf Name: FL_Region Units: unitless Description: <p>Name of the Florida&#039;s Coral Reef where sample was collected. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891146.rdf Name: Collaborator_siteID Units: unitless Description: <p>Name of reef if it falls under an existing monitoring program. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891147.rdf Name: Habitat Units: unitless Description: <p>Reef type. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891148.rdf Name: prochlorococcus_cells_per_ml Units: cells per milliliter Description: <p>Number of prochlorococcus cyanobacteria cells per milliliter in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891149.rdf Name: synechococcus_cells_per_ml Units: cells per milliliter Description: <p>Number of Synechococcus cyanobacteria cells per milliliter in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891150.rdf Name: picoeukaryotes_cells_per_ml Units: cells per milliliter Description: <p>Number of photosynthetic picoeukaryote cells per milliliter in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891151.rdf Name: unpigmented_cells_per_ml Units: cells per milliliter Description: <p>Number of unpigmented bacteria and archaea, also referred to as heterotrophic microbes, cells per milliliter in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891152.rdf Name: npoc_uM Units: micromolar (uM) Description: <p>Non-purgeable organic carbon, referred to as total organic carbon, in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891153.rdf Name: tn_uM Units: micromolar (uM) Description: <p>Total nitrogen, which includes organic and inorganic nitrogen. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891154.rdf Name: phosphate_uM Units: micromolar (uM) Description: <p>Phosphate in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891155.rdf Name: nitriteandnitrate_uM Units: micromolar (uM) Description: <p>Nitrite plus nitrate concentrations in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891156.rdf Name: silicate_uM Units: micromolar (uM) Description: <p>Silicate concentrations in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected; &quot;below detection&quot; = below detection limit</p> http://lod.bco-dmo.org/id/dataset-parameter/891157.rdf Name: nitrite_uM Units: micromolar (uM) Description: <p>Nitrite concentrations in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891158.rdf Name: ammonium_uM Units: micromolar (uM) Description: <p>Ammonium concentrations in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891159.rdf Name: nitrate_uM Units: micromolar (uM) Description: <p>Nitrate concentrations in reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected; &quot;below detection&quot; = below detection limit</p> http://lod.bco-dmo.org/id/dataset-parameter/891160.rdf Name: Temperature_C Units: degrees Celsius Description: <p>Temperature of seawater taken from Exo2 sonde or CastAway CTD (SonTek). &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891161.rdf Name: Dissolved_Oxygen_at_depth_percent_saturation Units: Percent saturation Description: <p>Dissolved oxygen of seawater taken from Exo2 sonde (SonTek). &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891162.rdf Name: Dissolved_Oxygen_at_depth_mg_per_liter Units: milligrams per liter (mg/L) Description: <p>Dissolved oxygen of seawater taken from Exo2 sonde (SonTek). &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891163.rdf Name: SCTLDprevalence Units: percent Description: <p>Percent of live coral on the reef with stony coral tissue loss disease. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891164.rdf Name: Calcified_Macroalgae Units: percent Description: <p>Amount of calcified macroalgae on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891165.rdf Name: Crustose_Coralline_Algae Units: percent Description: <p>Amount of crustose coralline algae on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891166.rdf Name: Cyanobacteria Units: percent Description: <p>Amount of cyanobacterial mats on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891167.rdf Name: Fleshy_Macroalgae Units: percent Description: <p>Amount of fleshy macroalgae on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891168.rdf Name: Hard_coral Units: percent Description: <p>Amount of hard coral on the reef</p> http://lod.bco-dmo.org/id/dataset-parameter/891169.rdf Name: Invertebrates Units: percent Description: <p>Amount of invertebrates on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891170.rdf Name: Non_biological Units: percent Description: <p>Amount of non-biological substrate on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891171.rdf Name: Other Units: percent Description: <p>Amount of other organisms and substrates on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891172.rdf Name: Other_invertebrates Units: percent Description: <p>Amount of other invertebrates on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891173.rdf Name: Seagrass Units: percent Description: <p>Amount of seagrass on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891174.rdf Name: Soft_coral Units: percent Description: <p>Amount of soft coral on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891175.rdf Name: Sponge Units: percent Description: <p>Amount of sponge on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891176.rdf Name: Turf_Algae Units: percent Description: <p>Amount of turf algae on the reef. &quot;Not applicable&quot; = data is not relevant; &quot;Not calculated&quot; = image data was collected but has not been used to calculate the parameter</p> http://lod.bco-dmo.org/id/dataset-parameter/891177.rdf Name: Chlorophyll_mg_per_l Units: milligrams per liter Description: <p>Amount of chlorophyll-a in the reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891178.rdf Name: Phaeophytin_mg_per_l Units: milligrams per liter Description: <p>Amount of phaeophytin in the reef seawater. &quot;Not applicable&quot; = data is not relevant; &quot;Not collected&quot; = data not collected</p> http://lod.bco-dmo.org/id/dataset-parameter/891179.rdf Name: coral Units: unitless Description: <p>Four-letter AGGRA code indicating the coral species from which the tissue or near-coral seawater sample was collected. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891180.rdf Name: healthbinary Units: unitless Description: <p>binary indicator of apparent condition of the coral colony with H = healthy and D = stony coral tissue loss disease. A third category of BS = black spot for any Siderastrea spp. corals that appeared to have a different disease. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891181.rdf Name: libraryID Units: unitless Description: <p>Letter indicating the sequencing library the sample was added to. Multiple sequencing libraries were used to accommodate all the samples so we aimed to record this in case of any sequencing run bias. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891182.rdf Name: sampleID Units: unitless Description: <p>Another sample ID specific to the near-coral seawater and coral microbiome dataset within the project. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891183.rdf Name: coralspecies Units: unitless Description: <p>Genus species of corals sampled. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891184.rdf Name: sworcoral Units: unitless Description: <p>Description of whether the sample was from Coral = slurry of mucus, tissue, skeleton, and seawater, or Near-coral seawater = 50 ml syringe sample from less than 5 cm from the coral surface. &quot;Not applicable&quot; = data is not relevant</p> http://lod.bco-dmo.org/id/dataset-parameter/891185.rdf Name: healthlong Units: unitless Description: <p>For samples in the coral and near-coral seawater microbiome dataset, description of the apparent health state of the coral, or whether the sample was a laboratory control. &quot;Not applicable&quot; = data is not relevant</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 214625 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/439b86bc-5779-5c70-a395-6a897f63fca8/content download https://doi.org/10.26008/1912/bco-dmo.890979.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Study Area:&lt;/strong&gt; We sampled coral reef environments during a research cruise aboard the M/V Alucia between June 5 and June 19, 2019. During this time, we conducted surveys and sampled biogeochemical seawater parameters at 85 reefs across 8 zones in Florida's Coral Reef, from the North Key Largo/Biscayne Bay area, designated as Zone 1, to the Dry Tortugas National Park (Zone 8). We selected reefs based on input from the Florida Fish and Wildlife Conservation Commission and existing reefs in long-term monitoring programs (e.g., Coral Reef Evaluation and Monitoring Project, CREMP).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sample Collection and Ship-board Processing:&lt;/strong&gt; We conducted diver-based surveys to evaluate the prevalence of stony coral tissue loss disease (SCTLD) at each of the 85 reefs. At each site, one diver performed a 30-minute roving diver survey to determine the richness of scleractinian species, the presence or absence of stony coral tissue loss disease, and the size of all observed coral colonies. The diver assigned coral colonies to four size classes, based on diameter/length: &amp;lt;10 centimeters (cm), 10-25cm, 25-50cm, and &amp;gt;50cm. The area in square meters (m2) surveyed by each diver was estimated to calculate the density of corals at each site.&lt;/p&gt; &lt;p&gt;We generated 100 m2 plots at each of the 85 reefs for benthic surveys via high-resolution 2D orthophotomosaics. We analyzed 45 of these reefs for benthic composition data after determining this would sufficiently cover the observed variability in the different reefs.&lt;/p&gt; &lt;p&gt;We collected discrete seawater samples at all 85 reefs to measure inorganic nutrient (phosphate, ammonium, silicate, nitrite plus nitrate) concentrations, total organic carbon (TOC) and total nitrogen (TN) concentrations, and cell abundances (heterotrophic microbes (unpigmented bacteria and archaea), &lt;em&gt;Prochlorococcus&lt;/em&gt;, &lt;em&gt;Synechococcus&lt;/em&gt;, and picoeukaryotes). We collected samples via SCUBA with acid-washed and combusted 40-milliliter (mL) borosilicate glass vials for TOC and TN collections and 30 mL acid-washed square bottles (HDPE, Nalgene, ThermoFisher Scientific, Waltham, MA, USA) for nutrient collections, and filled both vials while at reef depth. Samples were kept on ice in a cooler for less than 4 hours prior to processing. Once on board the M/V Alucia, we processed all samples. We added 75 microliters (μL) of phosphoric acid to the 40 mL glass vials to fix the samples for TOC and TN, and kept these samples at room temperature or 4°C until laboratory analysis. We removed 1.4 mL of seawater from the nutrient bottles, mixed it with 8% paraformaldehyde (1% final concentration, Electron Microscopy Sciences), fixed it in the dark for 20 minutes at 4°C, then froze it at -80°C. We capped the 30 mL inorganic nutrient bottles and placed them at -80°C until analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Metabolomic Analyses:&lt;/strong&gt; We collected seawater for targeted and untargeted metabolomic analyses at 13 reefs across the 8 zones of Florida’s Coral Reef. At each reef, we collected seawater in 1.7L Niskin bottles via SCUBA at three distinct locations on the reef for biological replication. These Niskin bottles were kept in a cooler for less than 4 hours prior to processing on the M/V Alucia. Once back on board the M/V Alucia, we transferred seawater from the Niskin bottles into acid-washed 2L polycarbonate bottles using acid-washed PharMedBPT tubing (Masterflex, Cole-Parmer, Vernon Hills, IL, USA). These water samples were processed as described previously by Weber et al. (2020). Briefly, we prefiltered the seawater through a 47-millimeter (mm) 0.1-micrometer (μm) pore size polytetrafluoroethylene filter (Omnipore, EMD Millipore Corporation, Billerica, MA, USA) to remove all microbial biomass via peristalsis and placed the filtered seawater directly into a second acid-washed 2L polycarbonate bottle. We acidified this filtrate with 2 mL OPTIMA-grade 12 M (molar) hydrochloric acid prior to solid phase extraction (SPE). We used SPE to concentrate metabolites (primarily low molecular weight dissolved organic matter) from the filtered seawater. We used a Waters vacuum manifold to slowly pass the seawater through 1 gram per 6 cubic centimeters (g/cc) SPE cartridges (Bond Elut PPL; Agilent, Santa Clara, CA, United States) pre-conditioned with HPLC-grade methanol and weighed bottles with seawater prior to and following SPE to calculate the volume of seawater filtered. SPE cartridges were wrapped in combusted aluminum foil and frozen to -80°C prior to analysis at the Woods Hole Oceanographic Institution.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Microbial Biomass and Chlorophyll Analyses&lt;/strong&gt;: We collected seawater for microbial biomass and chlorophyll analysis at 27 reefs across the 8 zones of Florida’s Coral Reef. At each reef, we employed a groundwater pump (Mini-Monsoon 12V, Proactive Environmental Products, Bradenton, Florida, USA) to pump seawater from just above the reef benthos into acid-washed or 10% bleach-rinsed 4L LDPE bottles (Nalgene). Samples were kept in a cooler on ice until processing less than 4 hours following collection. Once back on board the M/V Alucia, we used peristalsis to filter 2L of seawater to obtain duplicates from each reef through a 0.2 μm Supor filter (Pall, Port Washington, New York, USA) for microbial biomass housed in a 25 mm filter holder (Swinnex-25, Millipore Corporation), as described previously (Becker et al. 2020). Chlorophyll samples were obtained by filtering 2L of seawater in duplicate with the same peristalsis setup, but using a GF/F filter. We placed filters (GF/F or 0.2 μm) into 2 mL cryovials and froze them at -80°C prior to further processing at the Woods Hole Oceanographic Institution.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Coral Tissue Analyses:&lt;/strong&gt; On reefs with active stony coral tissue loss disease, we collected coral tissue and near-coral seawater samples from apparently healthy and actively diseased coral colonies. We aimed to only sample reefs where at least three healthy coral colonies were present in addition to at least three diseased colonies. Sample collection proceeded on near-coral seawater followed by coral tissue as described previously (Becker et al. 2021). Prior to tissue collection, we collected near-coral seawater via a 60 mL syringe within 1-5 cm of the lesion margin on diseased corals or healthy tissue from apparently healthy colonies. Following near-coral seawater collections, we sampled tissue of diseased corals at the lesion margin between apparently healthy tissue and bleached and sloughing tissue or apparently healthy colonies (without any indication of disease or other affliction) randomly on the coral head. We collected tissue samples with Luer slip 10 mL syringes. Syringes were quickly placed into small Whirl-Pak bags to contain any mucus and tissue leaking from the syringe. Following the collections, all tissue and near-coral seawater syringe samples were placed on ice prior to processing. We transferred samples of coral tissue and mucus into 15 ml conical tubes and froze them at -80°C until analysis. We attached filter holders containing 25 mm 0.2 μm Supor filters to the 60 mL luer-lock syringes and depressed them by hand to capture microbial biomass on the filters. We placed filters into labeled 2 mL cryovials and placed them in a -80°C freezer until analysis.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Disease prevalence and benthic composition:&lt;/strong&gt; Stony coral tissue loss disease (SCTLD) prevalence was calculated by dividing the number of coral colonies showing active disease by the total number of colonies on the reef. Benthic composition was analyzed by using 2,500 stratified random points that were placed over the high-resolution photomosaic and classified to generate reef-wide cover estimates of calcified macroalgae, crustose coralline algae, cyanobacteria, fleshy macroalgae, hard coral, invertebrates, non-biological substrate, other, other invertebrates, seagrass, soft coral, sponge, and turf algae. Full methods for benthic coverage estimates are in Fox et al. (2019).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Flow cytometry, organic nutrient, inorganic nutrient, and chlorophyll analyses for water quality:&lt;/strong&gt; Flow cytometry samples were processed and analyzed by the University of Hawaii SOEST Flow Cytometry Facility as described previously (Becker et al 2020). Briefly, each sample was stained with Hoechst 33342 DNA stain and excited with both 488 nanometers (nm) (1W) and UV (~350 nm, 200 mW) lasers co-linearly on a Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences). Signals of forward and side scatter and fluorescence were analyzed to distinguish populations and abundances (cells ml−1) of four cell types: &lt;em&gt;Prochlorococcus&lt;/em&gt;, &lt;em&gt;Synechococcus&lt;/em&gt;, eukaryotic picophytoplankton (picoeukaryotes), and non-pigmented bacteria. Non-pigmented prokaryotes were used as a proxy for heterotrophic bacterial and archaeal cells (Monger and Landry 1993, Marie et al. 1997).&lt;/p&gt; &lt;p&gt;Non-purgeable total organic carbon (TOC) samples and total nitrogen (TN) samples were run with a Shimadzu TOC-VCSH TOC analyzer (Hansell and Carlson, 2001) using a TNM-1 module. We shipped inorganic nutrient samples to Oregon State University for analysis of phosphate, ammonium, silicate, nitrite, and nitrate, as in Apprill and Rappé (2011). Briefly, samples were run on a Technicon AutoAnalyzer II (SEAL Analytical) and an Alpkem RFA 300 Rapid Flow Analyzer to generate nutrient concentrations (μM). We generated total organic nitrogen (TON) concentrations by subtracting concentrations of inorganic nitrogen (ammonium and nitrite plus nitrate) from total nitrogen.&lt;/p&gt; &lt;p&gt;Chlorophyll was extracted with acetone using standard methods (JGOFS, 1996). Filters were thawed individually and immediately placed in a glass test tube with 5 mL or 10 ml of 90% acetone, with 10 ml used in the case the filter appeared particularly dark, and capped. The filters were left to extract for 24 hours in the dark at 4°C. After the extraction, the tubes were vortexed and centrifuged to concentrate any particulate matter at the bottom of the tube. Prior to analysis, blanks including air, 90% acetone, and a black standard were run on an AquaFluor fluorometer (Turner Designs handheld 800446) fitted with a red-sensitive photomultiplier. Approximately 3 mL of solvent was analyzed on the fluorometer at wavelength of 664 nm, followed by acidifying the sample with two drops of 10% hydrochloric acid, then measuring again to assess phaeopigment concentration. Readings were corrected for the volume filtered and concentration of chlorophyll was measured by referencing a standard curve.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Targeted and untargeted metabolomic laboratory processing and mass spectrometry:&lt;/strong&gt; Untargeted samples were run 2/5/2020. We eluted dissolved organic matter (DOM) from the SPE cartridges and prepared samples for analyses as outlined by Weber and colleagues (2020). To summarize, 4 bed-volumes of 0.01 M HCl were added to the cartridges to remove salt. The cartridges were then dried for five minutes, and eluted into combusted glass vials using 6 mL of 100% methanol. Extracts were frozen at -20ºC until they were dried down using a vacuum centrifuge. Extracts were then resuspended with a 95:5 (v/v) MilliQ water: acetonitrile (ACN) solution with deuterated biotin (final concentration 0.05 milligrams per milliliter (mg per mL) (200 μL total) and vortexed. Pooled samples were made for all mass spectrometry runs by combining equal volumes of all extracts into one vial. The pooled samples were used for monitoring instrument drift and run quality. After preparation, all extracts were stored at -20◦C until analysis. For targeted metabolomics, 100 μL aliquots of each extract was placed in a separate vial with a combusted glass insert. For the untargeted metabolomics analysis, 600 μL of the deuterated biotin standard and water: ACN solution was used to dilute a 25 µL aliquot of each extract. Untargeted metabolite analysis was performed using an ultrahigh-performance liquid chromatography system (Vanquish UHPLC, Thermo ScientificTM) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo ScientificTM). A Vanguard pre-column and Waters Acquity HSS T3 column (2.1 mm × 100 mm, 1.8 μm), was used for chromatographic separation at 40ºC. The column was eluted at 0.5 mL per minute with the following solvents: A) 0.1% formic acid in water and B) 0.1% formic acid in ACN. The chromatographic gradient was: 1% B for 1 min, 15% B for 1-3 minutes, 50% B for 3-6 minutes, 95% B for 6-9 minutes, and 95% B for 10 minutes. Between injections, the column was washed and re-equilibrated with 1% B for 2 minutes. Individual autosampler injections (5 μL each) were made for negative and positive ion mode analyses. In negative ion mode, the electrospray voltage was set to 2600 volts (V). Settings for source gases were 55 (sheath), 20 (auxiliary), and 1 (sweep) in arbitrary units. The temperatures of the heated capillary and vaporizer were 350ºC and 400ºC, respectively. MS data were collected in the Orbitrap analyzer with a mass resolution of 120,000 FWHM at m/z 200. The automatic gain control (AGC) target was 4e5, with a 50 sec maximum injection time, and a scan range of 100 to 1000 m/z. Data-dependent MS/MS spectra were collected at 7,500 resolution in the Orbitrap analyzer. Parent ions were isolated with a 1 m/z width in the quadrupole, and fragmented with a HCD (higher energy collisional dissociation) energy of 35%. All data were collected in profile mode. Samples were run in random order and after every seven samples, a pooled sample was run. Raw data files from the instrument were converted into mzML files using msConvert and then processed using XCMS (Smith et al., 2006). Peak-picking was performed with the CentWave algorithm and a Gaussian fit with the following parameters: noise = 10000, peak-width = 3-15, ppm = 15, prefilter = c(2,168.600), integrate = 2, mzdiff = -0.005, snthresh = 10. Retention times were then adjusted using Orbiwarp and correspondence between the peaks was conducted. The coefficient of variation across the eight untargeted pooled sample features was 0.044, demonstrating good agreement between the pooled samples, and the pooled samples were removed from further analyses. Untargeted peak intensities were normalized by dividing the peak intensities by the total seawater volume. In the untargeted analysis, only MS1 features were analyzed, and were defined as unique combinations of mass-to-charge ratios (m/z) and retention times (RT). This analysis yielded a table of MS1 features (m/z x RT) and their peak intensities across each sample.&lt;/p&gt; &lt;p&gt;Extracts prepared for targeted metabolomics were run on a triple-stage quadrupole mass spectrometer (TSQ Vantage, Thermo Fisher ScientificTM) using UPLC (Accela Open Autosampler and Accela 1250 Pump, Thermo ScientificTM) coupled to a heated electrospray ionization source (H-ESI) and operated in selective reaction monitoring (SRM) mode. The same chromatography column, conditions, gradient, and flow rates were used for targeted analyses as those described for untargeted analyses. Separate autosampler injections of 5 μL each were made for positive and negative modes. Additionally, as with the untargeted analysis, samples were run in random order and pooled samples were run every seven samples. SRM parameters were optimized for each compound using a standard as described in Kido Soule et al. (2015) and two SRM transitions (precursor-product ion pairs) were monitored for quantification and confirmation. Target metabolites included compounds found in central carbon metabolism and metabolites that are environmentally relevant in marine habitats or are produced by marine microorganisms (Fiore et al. 2015, Fiore et al. 2017, Kido Soule et al. 2015). The resulting XCalibur raw files (MS/MS data) were converted into mzML files using msConvert (Chambers et al., 2012) and processed with the open-source program El-MAVEN (v.774)(Agrawal et al., 2019) . Using El-MAVEN, 8-point calibration curves based on integrated peak area were generated for each compound. Environmental concentrations of metabolites were determined by dividing each concentration by the original sample collection volume. Next, metabolites that passed the limits of detection and quantification for the UPLC-MS/MS analysis (Kido Soule, Longnecker, Swarr, unpublished) were corrected for extraction efficiency based on published data for each metabolite in seawater (Johnson et al., 2017).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;DNA extraction and sequencing for 16S rRNA and shotgun metagenomes:&lt;/strong&gt; We extracted DNA from 25 mm filters used for 2L seawater collections using Qiagen PowerBiofilm kits (Qiagen, Germantown, MD, USA). To begin, we added the filter directly to the bead tube, then proceeded with the extraction following manufacturer protocols. We also included four DNA extraction controls by extracting DNA from unused filters. Resulting DNA from these extractions was used as the template for both 16S rRNA gene sequencing and shotgun metagenomic sequencing.&lt;/p&gt; &lt;p&gt;For 16S rRNA gene sequencing of bacteria and archaea, we included 2 μL of template DNA into a 50 μL (total volume) PCR reaction. We added a PCR negative control by including one PCR reaction with 2 μL of PCR grade H2O instead of DNA. Earth Microbiome Project primers, 515F (Parada et al., 2016) and 806R (Apprill et al., 2015), were used to amplify the V4 region of the small subunit (SSU) rRNA gene in bacteria and archaea and included sample-specific barcodes with an 8 bp barcode, 10 bp pad, and 2 bp link, similar to Kozich et al. (2013). The 50 μL reactions were diluted in UV-sterilized nuclease-free water and contained 2.5 units of GoTaq DNA Polymerase (Promega, Madison, WI, USA), barcoded primers at 0.2 μM, 0.2 mM dNTP mix (Promega), 2.5 mM MgCl2, and 1X colorless GoTaq flexi buffer (Promega). The reactions were run on a Bio-Rad Thermocycler using the following criteria: denaturation at 95°C for 2 minutes; 28 cycles at 95°C for 20 seconds, 55°C for 15 seconds, and 72°C for 5 minutes; and extension at 72°C for 10 minutes. We used gel electrophoresis to verify successful amplification using 5 μL of product on a 1% agarose-Tris-borate-EDTA (TBE) gel stained with SYBR Safe gel stain (Invitrogen, ThermoFisher Scientific). We used the QIAquick 96 PCR Purification Kit (Qiagen) with the QIAvac 96 (Qiagen) and vacuum pressure to purify the remaining 45 μL of PCR products following manufacturer’s protocols. We applied the HS dsDNA assay on the Qubit 2.0 fluorometer (ThermoFisher Scientific) to quantify the DNA concentrations then converted to nM assuming an average library size of 450 bp, and average molar mass of DNA nucleotides of 660 g/mol. We diluted individual barcoded PCR products to 10 nM, pooled all samples, and shipped the pooled, ready-to-run library to the Georgia Genomics and Bioinformatics Core at the University of Georgia for sequencing on an Illumina MiSeq using paired-end 250 bp sequencing.&lt;/p&gt; &lt;p&gt;We prepared a library for shotgun metagenomic sequencing following the Illumina DNA Prep Reference Guide (Illumina, San Diego, CA, USA, Document # 1000000025416 v09 June 2020). DNA input for all samples was between 100-500 ng. Concentration of the four DNA extraction control samples was below detection, so we included 30 μL of each in the procedure and processed in the same way as all seawater samples. We used IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation (Illumina, 96 samples, Cat # 20027213), to apply sample-specific indices to each sample. Following the procedure, we eluted samples in 30 μL resuspension buffer. To verify successful processing, we used a fluorometric assay (HS dsDNA) on a Qubit 2.0 fluorometer to measure DNA concentrations of a subset of samples. All final concentrations were greater than 4 ng/μL, and therefore deemed sufficient for pooling. All samples were pooled, and the final concentration was 5.30 ng/μL. The final library was run at the Georgia Genomics and Bioinformatics Core at the University of Georgia on an Illumina NextSeq 2000 with the P3 flow cell and paired-end 150 bp sequencing.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;On-ship near-coral seawater sample processing and sequencing:&lt;/strong&gt; To expedite the turnaround time between sample collection and sample processing, we performed on-ship DNA extraction, PCR, and sequencing with the Illumina iSeq 100 System on near-coral seawater samples following methods for in-the-field microbiome preparation described previously (Becker et al., 2021). Seawater samples targeted for on-ship sequencing included those sampled from 5 reefs over two days (June 9-10, 2019).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;DNA extraction and sequencing of near-coral seawater and coral microbiomes:&lt;/strong&gt; All processing of near-coral seawater and coral tissue slurries proceeded as described previously to identify bacteria and archaea within each environment (Becker et al., 2021). Briefly, we extracted DNA from all seawater and tissue samples using the DNeasy PowerBiofilm kit (Qiagen). PCR occurred in a two-stage procedure. In stage one, we used Earth Microbiome Project primers, 515F (Parada et al., 2016) and 806R (Apprill et al., 2015), to amplify the V4 region of the small subunit (SSU) ribosomal RNA gene of bacteria and archaea. In stage two, we attached unique index primers to each sample using the Nextera XT v2 set A kit (Illumina). For PCR that occurred at the Woods Hole Oceanographic Institution, we used larger benchtop centrifuges (Eppendorf 5418) and thermocyclers (Bio-Rad), rather than the small and portable versions used on the M/V Alucia. Following purification of stage two PCR products, we diluted and pooled samples such that we included approximately 40 samples. Seawater and tissue samples were randomized across all library pools. Pooled libraries were diluted to approximately 90 picomolar (pM), and a 10% PhiX Control v3 (Illumina) spike-in was added to increase base diversity. All libraries were run on the Illumina iSeq 100 System (Illumina) with the i1 cartridge pack, over a total of 6 sequencing runs.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Illumina MiSeq Illumina MiSeq PI Supplied Instrument Name: Illumina MiSeq PI Supplied Instrument Description:Illumina MiSeq using paired-end 250 bp sequencing - for 16S rRNA gene sequencing libraries of bulk reef seawater Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences. Illumina NextSeq 2000 Illumina NextSeq 2000 PI Supplied Instrument Name: Illumina NextSeq 2000 PI Supplied Instrument Description:Illumina NextSeq 2000 with the P3 flow cell and paired-end 150 bp sequencing for shotgun metagenomics Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences. Illumina iSeq 100 System Illumina iSeq 100 System PI Supplied Instrument Name: Illumina iSeq 100 System PI Supplied Instrument Description:Illumina iSeq 100 System (Illumina) with the i1 cartridge, and 2x150 bp sequencing for 16S rRNA libraries of coral and near-coral seawater Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences. Beckman-Coulter Altra flow cytometer Beckman-Coulter Altra flow cytometer PI Supplied Instrument Name: Beckman-Coulter Altra flow cytometer PI Supplied Instrument Description:Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences) for enumerating cell abundances Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Accela Open Autosampler Accela Open Autosampler PI Supplied Instrument Name: Accela Open Autosampler PI Supplied Instrument Description:Triple stage quadrupole mass spectrometer (TSQ Vantage, Thermo Fisher ScientificTM) using UPLC (Accela Open Autosampler and Accela 1250 Pump, Thermo ScientificTM) coupled to a heated electrospray ionization source (H-ESI) for targeted metabolite analysis Instrument Name: Laboratory Autosampler Instrument Short Name: Instrument Description: Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument. Orbitrap Fusion Lumos Tribid mass spectrometer Orbitrap Fusion Lumos Tribid mass spectrometer PI Supplied Instrument Name: Orbitrap Fusion Lumos Tribid mass spectrometer PI Supplied Instrument Description:Ultrahigh performance liquid chromatography system (Vanquish UHPLC, Thermo ScientificTM) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo ScientificTM) for untargeted metabolomics Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ Niskin bottles Niskin bottles PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:1.7 Liter Niskin bottles - seawater sampling Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ groundwater pump groundwater pump PI Supplied Instrument Name: groundwater pump PI Supplied Instrument Description:Mini-Monsoon 12V, Proactive Environmental Products, Bradenton, Florida, USA - to pump seawater from just above the reef benthos Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps TSQ Vantage, Thermo Fisher ScientificTM TSQ Vantage, Thermo Fisher ScientificTM PI Supplied Instrument Name: TSQ Vantage, Thermo Fisher ScientificTM PI Supplied Instrument Description:Triple stage quadrupole mass spectrometer (TSQ Vantage, Thermo Fisher ScientificTM) using UPLC (Accela Open Autosampler and Accela 1250 Pump, Thermo ScientificTM) coupled to a heated electrospray ionization source (H-ESI) for targeted metabolite analysis Instrument Name: Quadrupole Mass Spectrometer Instrument Short Name:QMS Instrument Description: A piece of apparatus that consists of an ion source, a mass-to-charge analyser, a detector and a vacuum system and is used to measure mass spectra. The detector is a quadrupole mass-to-charge analyser, which holds the ions in a stable orbit by an electric field generated by four parallel electrodes.   luer slip and luer lock syringes luer slip and luer lock syringes PI Supplied Instrument Name: luer slip and luer lock syringes PI Supplied Instrument Description:10 mL luer slip and 60 mL luer lock syringes to collect coral tissue and near coral seawater, respectively Instrument Name: syringe Instrument Short Name: Instrument Description: A device used to inject fluids into or withdraw them from something; consists of a hollow barrel fitted with a plunger and a hollow needle. Technicon AutoAnalyzer II Technicon AutoAnalyzer II PI Supplied Instrument Name: Technicon AutoAnalyzer II PI Supplied Instrument Description:Technicon AutoAnalyzer II (SEAL Analytical) and an Alpkem RFA 300 Rapid Flow Analyzer to generate nutrient concentrations (μM) Instrument Name: Technicon AutoAnalyzer II Instrument Short Name: Instrument Description: A rapid flow analyzer that may be used to measure nutrient concentrations in seawater. It is a continuous segmented flow instrument consisting of a sampler, peristaltic pump, analytical cartridge, heating bath, and colorimeter. See more information about this instrument from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0291/ Shimadzu TOC-VCSH TOC analyzer Shimadzu TOC-VCSH TOC analyzer PI Supplied Instrument Name: Shimadzu TOC-VCSH TOC analyzer PI Supplied Instrument Description:Shimadzu TOC-VCSH TOC analyzer (6) using a TNM-1 module for TOC and TN analysis Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/ AquaFluor fluorometer AquaFluor fluorometer PI Supplied Instrument Name: AquaFluor fluorometer PI Supplied Instrument Description:AquaFluor fluorometer (Turner Designs handheld 800446) fitted with a red sensitive photomultiplier for Chlorophyll analysis Instrument Name: Turner Designs Aquafluor 8000 Instrument Short Name:Turner Designs Aquafluor 8000 Instrument Description: The Turner Designs Aquafluor 8000 is a lightweight, handheld fluorometer/turbidimeter ideal for field use. It can be configured with one or two channels to measure turbidity, chlorophyll, algae, dyes, ammonium, CDOM, and more. Detailed description at https://www.turnerdesigns.com/aquafluor-handheld-fluorometer Vanquish UHPLC, Thermo ScientificTM Vanquish UHPLC, Thermo ScientificTM PI Supplied Instrument Name: Vanquish UHPLC, Thermo ScientificTM PI Supplied Instrument Description:Ultrahigh performance liquid chromatography system (Vanquish UHPLC, Thermo ScientificTM) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo ScientificTM) for untargeted metabolomics Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high. Waters Vacuum Manifold (Agilent) Waters Vacuum Manifold (Agilent) PI Supplied Instrument Name: Waters Vacuum Manifold (Agilent) PI Supplied Instrument Description:Waters vacuum manifold and 1 g/6 cc SPE cartridges (Bond Elut PPL; Agilent, Santa Clara, CA, United States) for concentrating seawater dissolved metabolites Instrument Name: vacuum manifold Instrument Short Name: Instrument Description: A device that is used for the vacuum-driven processing of multiwell strips or plates, or spin columns. Cruise: OceanX OceanX Community Standard Description International Council for the Exploration of the Sea Alucia vessel Community Standard Description International Council for the Exploration of the Sea Alucia vessel