http://lod.bco-dmo.org/id/dataset/892576
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-03-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021
2023-04-10
publication
2023-04-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-04-12
publication
https://doi.org/10.26008/1912/bco-dmo.892576.1
James L. Pinckney
University of South Carolina
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Pinckney, J. L. (2023) Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.892576.1 [access date]
Methods and Sampling: <p>Samples for Benthic microalgal photopigment analysis were collected by SCUBA divers from the R/V <em>Trinity</em> in nearshore waters of Charleston, SC using 1 square centimeter x 10 centimeter (cm) plastic core tubes gently pushed into the sediment to minimize disruption of the surface layers. Tubes were capped and carefully removed from the sediment. Ten cores were obtained randomly within a 1 square meter area at select locations for each sample date. The upper 1 centimeter of the core was extruded, frozen at -80 degrees Celsius, and analyzed within 30 days after collection. Sediments were dried and sieved for grain size analysis.</p>
<p>High-performance liquid chromatography (HPLC) was used to determine chemosystematic photosynthetic pigments. Samples were lyophilized for 24 hours at -50 degrees Celsius, placed in 90 percent acetone (1 milliliter), sonicated on ice for 30 seconds, and extracted at -20 degrees Celsius for 18 to 20 hours. Filtered extracts: 0.45 micrometers, 250 microliters were injected into a Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 centimeters, 3 micrometers) and a polymeric (Vydac 201TP54, 0.46 x 25 centimeters, 5 micrometers) reverse-phase C18 column in series. A nonlinear binary gradient of 80&nbsp;percent methanol: 20&nbsp;percent 0.50 M ammonium acetate and 80 percent methanol:20&nbsp;percent acetone was the mobile phase (Pinckney et al. 1996, 2001). Absorption spectra and chromatograms (440 ± 4 nanometers) were acquired using a Shimadzu SPD-M10av photodiode array detector. Pigment peaks were identified by comparison of retention times and absorption spectra with pure carotenal and chlorophyll standards (DHI, Denmark). The synthetic carotenoid β-apo-8'-carotenal (Sigma) was used as an internal standard.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736557 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736557
completed
James L. Pinckney
University of South Carolina
803-546-0768
Baruch Institute
Columbia
SC
29208
USA
pinckney@sc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Location
Cruise_Number
Latitude
Longitude
Date
Depth
Fucoxanthin
Zeaxanthin
Chlorophyll_b
Chlorophyll_a
Shimadzu 10AD HPLC
theme
None, User defined
station
station id
latitude
longitude
date
depth core
fucoxanthin
zeaxanthin
chlorophyll b
chlorophyll a
featureType
BCO-DMO Standard Parameters
Self-Contained Underwater Breathing Apparatus
Ultra-high-performance liquid chromatography
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Groundwater sources of "new" N for benthic microalgal production in the South Atlantic bight
https://osprey.bco-dmo.org/project/700280
Groundwater sources of "new" N for benthic microalgal production in the South Atlantic bight
<p><em>NSF Award Abstract:</em><br />
Continental shelves are highly productive, with both ecological and economic importance. Benthic microalgae (BMA) are key primary producers in these location. As much as 6x the water column biomass of primary producers is compressed into a layer only a few mm thick on the sediment surface. The source(s) of fixed nitrogen (N) supporting such highly concentrated BMA biomass is currently unknown. Recent studies of sub-seafloor groundwater flow at the University of South Carolina have demonstrated that upwelling saline groundwater likely supplies high concentrations of nutrients in the ridge-swale habitats in the South Atlantic Bight (SAB). The investigators suggest that groundwater input of fixed N into surficial sediments is the primary source of N supporting BMA biomass and production in the mid-shelf region of the SAB. The purpose of this project is to determine the primary source of fixed N supporting BMA biomass in the surface sediments of the shallow shelf waters (<30 m), using the SAB as a field area. A secondary objective is to apply novel and innovative methods to directly quantify groundwater inputs of N into surficial sediments. Research results will fully document the spatio-temporal distributions of BMA and phytoplankton biomass and community structure in the mid-shelf region of the SAB and relate the observed patterns to groundwater inputs of fixed N sources as well as hydrographic and climatic conditions. This research will provide full support and tuition for 2 graduate students, summer support for undergraduate assistants, and involve upper level undergraduates as lab interns. The study team will also work with the Baruch Institute and other partners to develop an "Ocean Schoolyard" program to meet the needs of teachers, students, and community audiences. The project will also provide partial support for Girls Go for I.T., a coding summer camp designed to attract middle-school-aged girls to careers in I.T. and STEM fields.</p>
<p>The specific objectives of the study are to (1) quantify spatial and temporal variations in N fluxes associated with hydrodynamic exchange and upward groundwater flow (2) document spatial and temporal variations in BMA biomass and (3) measure the delta15N of fixed nitrogen sources (well water, porewater and water column ammonium and nitrate; sediments), the BMA, and phytoplankton. The sampling area will be restricted to the 10 - 30 m isobath region of the SAB off the coast of Charleston, SC. Samples will be collected at both the existing groundwater well field and other regions of the shelf. At each of the groundwater wells in the well field, SCUBA divers will collect fluids from the wells to determine well water inorganic nutrient concentrations (nitrate + nitrite, ammonium, orthophosphate, silicon) and the delta 15N of well water ammonium and nitrate (when present). In nearby sediments, samples will be collected for BMA biomass and community composition, surface porewater inorganic nutrients (nitrate + nitrite, ammonium, orthophosphate, silicon), C and N of sediments, sediment grain size analysis, and delta 15N of BMA, ammonium, nitrate (when present), and sediments. Line transects, consisting of 5 sampling locations along a 50 m transect, will be conducted in each of the 4 depth strata. At 10 m intervals along each transect, divers will collect samples the same as above for the well field. Water column samples will be collected for HPLC measurements of phytoplankton biomass and community composition, inorganic nutrient concentrations (nitrate + nitrite, ammonium, orthophosphate, silicon), seston CHN, delta 15N of phytoplankton, and the delta 15N of ammonium and nitrate. The researchers will use heat as a tracer to map the depth of hydrodynamic exchange and monitor the rate of vertical groundwater flow. Results from that analysis will also allow them to then simulate transport of a conservative tracer that can be compared to observed nutrient concentrations to BMA abundance and community composition.</p>
SAB BMA
largerWorkCitation
project
eng; USA
biota
oceans
-79.6632
-79.2124
32.5862
32.7878
2018-06-07
2021-08-10
South Atlantic Bight (32 N, 79 W)
0
BCO-DMO catalogue of parameters from Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/892987.rdf
Name: Location
Units: unitless
Description: <p>Sample location identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/892988.rdf
Name: Cruise_Number
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/892989.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling site North (South is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/892990.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling site East (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/892991.rdf
Name: Date
Units: unitless
Description: <p>Date of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/892992.rdf
Name: Depth
Units: millimeters (mm)
Description: <p>Depth of core sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/892993.rdf
Name: Fucoxanthin
Units: micrograms per gram of dry sediment
Description: <p>Fucoxanthin pigment</p>
http://lod.bco-dmo.org/id/dataset-parameter/892994.rdf
Name: Zeaxanthin
Units: micrograms per gram of dry sediment
Description: <p>Zeaxanthin pigment</p>
http://lod.bco-dmo.org/id/dataset-parameter/892995.rdf
Name: Chlorophyll_b
Units: micrograms per gram of dry sediment
Description: <p>Chlorophyll b pigment</p>
http://lod.bco-dmo.org/id/dataset-parameter/892996.rdf
Name: Chlorophyll_a
Units: micrograms per gram of dry sediment
Description: <p>Chlorophyll a pigment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
31588
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/795c1fb1-7210-48a2-86a2-2e5a5c153de1/content
download
https://doi.org/10.26008/1912/bco-dmo.892576.1
download
onLine
dataset
<p>Samples for Benthic microalgal photopigment analysis were collected by SCUBA divers from the R/V <em>Trinity</em> in nearshore waters of Charleston, SC using 1 square centimeter x 10 centimeter (cm) plastic core tubes gently pushed into the sediment to minimize disruption of the surface layers. Tubes were capped and carefully removed from the sediment. Ten cores were obtained randomly within a 1 square meter area at select locations for each sample date. The upper 1 centimeter of the core was extruded, frozen at -80 degrees Celsius, and analyzed within 30 days after collection. Sediments were dried and sieved for grain size analysis.</p>
<p>High-performance liquid chromatography (HPLC) was used to determine chemosystematic photosynthetic pigments. Samples were lyophilized for 24 hours at -50 degrees Celsius, placed in 90 percent acetone (1 milliliter), sonicated on ice for 30 seconds, and extracted at -20 degrees Celsius for 18 to 20 hours. Filtered extracts: 0.45 micrometers, 250 microliters were injected into a Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 centimeters, 3 micrometers) and a polymeric (Vydac 201TP54, 0.46 x 25 centimeters, 5 micrometers) reverse-phase C18 column in series. A nonlinear binary gradient of 80&nbsp;percent methanol: 20&nbsp;percent 0.50 M ammonium acetate and 80 percent methanol:20&nbsp;percent acetone was the mobile phase (Pinckney et al. 1996, 2001). Absorption spectra and chromatograms (440 ± 4 nanometers) were acquired using a Shimadzu SPD-M10av photodiode array detector. Pigment peaks were identified by comparison of retention times and absorption spectra with pure carotenal and chlorophyll standards (DHI, Denmark). The synthetic carotenoid β-apo-8'-carotenal (Sigma) was used as an internal standard.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Description:</strong><br />
- Longitude values were converted to decimal degrees where negative values denote the Southern hemisphere</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Self-Contained Underwater Breathing Apparatus Instrument Short Name:SCUBA Instrument Description: The self-contained underwater breathing apparatus or scuba diving system is the result of technological developments and innovations that began almost 300 years ago. Scuba diving is the most extensively used system for breathing underwater by recreational divers throughout the world and in various forms is also widely used to perform underwater work for military, scientific, and commercial purposes.
Reference: https://oceanexplorer.noaa.gov/technology/technical/technical.html
Shimadzu 10AD HPLC
Shimadzu 10AD HPLC
PI Supplied Instrument Name: Shimadzu 10AD HPLC PI Supplied Instrument Description:Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 µm) reverse-phase C18 column in series. Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.