http://lod.bco-dmo.org/id/dataset/892660
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-03-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Ramped pyrolysis oxidation (RPO) carbon isotope data from DOC in water samples collected during R/V Western Flyer Benthic-Pelagic Coupling expedition to Station M in the eastern Pacific Ocean in April 2018
2023-03-27
publication
2023-03-27
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-04-19
publication
https://doi.org/10.26008/1912/bco-dmo.892660.1
Margot Elizabeth White
University of California-San Diego Scripps
principalInvestigator
Lihini Aluwihare
University of California-San Diego Scripps
principalInvestigator
Steven R. Beaupré
Stony Brook University - SoMAS
principalInvestigator
John Michael Beman
University of California-Merced
principalInvestigator
Ann McNichol
Woods Hole Oceanographic Institution
principalInvestigator
Kenneth Smith
Monterey Bay Aquarium Research Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: White, M. E., Nguyen, T., Koester, I., Lardie Gaylord, M., Beman, J. M., Smith, K., McNichol, A., Beaupré, S. R., Aluwihare, L. (2023) Ramped pyrolysis oxidation (RPO) carbon isotope data from DOC in water samples collected during R/V Western Flyer Benthic-Pelagic Coupling expedition to Station M in the eastern Pacific Ocean in April 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.892660.1 [access date]
Methods and Sampling: <p><strong>Field collection of samples</strong><br />
Samples were collected during a cruise aboard the R/V Western Flyer to Station M off the coast of central California in April 2018 (34°<sup>&nbsp;</sup>50’N, 123°<sup>&nbsp;</sup>00’W; 4100 meters depth).&nbsp; 30 to 40 Liters of seawater were collected at five depths ranging from 45 to 3700 meters.&nbsp;Water was then acidified to pH 2&nbsp;using trace metal grade hydrochloric acid (HCl). Solid phase extraction columns (1g Bond Elut PPL cartridges by Agilent) were first activated overnight with liquid chromatography/mass spectrometry (LC/MS) grade methanol and then rinsed with LC/MS grade water, LC/MS grade methanol, and LC/MS grade water adjusted to pH 2 using trace metal grade HCl. The acidified seawater was passed through the PPL cartridges under gravitational pressure over 1-2 days. This PPL solid phase extraction method&nbsp;has been used extensively to isolate marine DOM (Dittmar et al., 2008; Petras et al., 2017 and more) and provides yields of 35-60%. Following acidification, the method preferentially extracts hydrophobic compounds but is also able to isolate some semi-polar compounds (Johnson et al., 2017; Petras et al., 2021) whose overall concentrations may change with depth. In addition to the relatively high yield, previous work has shown that Δ<sup>14</sup>C of PPL-DOC is similar to that of the bulk (Lechtenfeld et al., 2014; Lewis et al., 2021). Typically, 5L of seawater was extracted with one 1g PPL cartridge.</p>
<p>Before eluting the PPL resin, and immediately following the extraction, the cartridge was rinsed with three column volumes of pH 2 LC/MS grade water and dried under ultra-high purity nitrogen gas. Organic matter retained on the cartridge was then eluted with 10 mL of methanol/1 g PPL cartridge (PPL-DOC) and stored at -20 °C. Seawater for total organic carbon concentration [TOC] and dissolved organic carbon concentration [DOC] analysis (i.e., seawater pre-filtered through combusted Whatman corporation GF/F filters)&nbsp;was collected into combusted 40-mL borosilicate vials, immediately acidified to pH 2 using trace metal grade 12 N HCl (Fisher Scientific), capped with acid-washed vial caps with septa, and stored at room temperature until measurement.&nbsp;</p>
<p>A fraction of the extracted PPL-DOC sample was dried extensively to remove methanol and then subjected to a 2 M acid hydrolysis. Briefly, the organic matter was transferred to an ampoule with 2 mL of 2 M hydrochloric acid and sealed under a nitrogen atmosphere before being placed in a drying&nbsp;oven at 80°C for 18 hours. Following hydrolysis, the sample was diluted with LC/MS grade water to pH 2 and then re-extracted onto a PPL cartridge as described above. The organic matter retained by the cartridge was then eluted in methanol and dried down for frozen storage.&nbsp;</p>
<p><strong>Laboratory sampling</strong><br />
Ramped pyrolysis/oxidation (RPO) was performed at the National Ocean Sciences Accelerator Mass Spectrometry (NOSAMS) facility in Woods Hole, MA, USA. Samples were transferred while dissolved in a small amount of methanol into quartz cups (pre-combusted at 850 °C, 1 hour) and then dried down under ultra-high purity nitrogen gas. Samples were further dried in an oven at 50°C for multiple days in an attempt to remove all methanol. Each quartz cup containing a single sample was then placed inside the RPO reactor via a quartz insert tube. The RPO instrument continuously heats the sample at a set rate, monitors evolved CO<sub>2&nbsp;</sub>during the pyrolysis/oxidation reactions as “thermograms” (Rosenheim et al., 2008), and can be used to trap CO<sub>2&nbsp;</sub>for isotope analysis. (For Station M thermogram data, see BCO-DMO dataset here: https://www.bco-dmo.org/dataset/892564).&nbsp;The RPO methodology has been described previously and further information describing the detailed protocol can be found in other publications (Hemingway et al., 2017; Rosenheim et al., 2008). In summary, all samples were operated in oxidation mode (carrier gas 92% He, 8% O<sub>2</sub>) with a flow rate of 35 mL min<sup>−1</sup>&nbsp;and a ramp rate of either 20 or 5 degrees Celsius per minute. Samples analyzed at 20°C min<sup>−1&nbsp;</sup>ranged in size from 6.4 to 36.8 µmol C and all resulting CO<sub>2</sub> was collected for isotope analysis. Samples analyzed at 5°C min<sup>−1</sup>&nbsp;ranged in size from 48.2 to 185.2 µmol C, where these amounts represent the sums of six fractions collected during each 5°C min<sup>−1</sup>&nbsp;ramp, with each fraction ranging in size from 4.4 to 43.6 µmol C.&nbsp; In total we ran ten 20°C min<sup>−1</sup>&nbsp;ramps and six 5°C min<sup>−1</sup>&nbsp;ramps of samples from Station M.</p>
<p><strong>Isotope measurements</strong><br />
All bulk and RPO-fraction isotope measurements were performed at NOSAMS. Stable carbon isotope compositions were measured on the resultant CO<sub>2</sub> gas using a dual-inlet isotope ratio mass spectrometer (IRMS), with resulting <sup>13</sup>C content expressed in δ<sup>13</sup>C per mil (‰) notation relative to Vienna Pee Dee Belemnite (VPDB). Radiocarbon measurements were completed via accelerator mass spectrometry (AMS) following standard graphitization methods (McNichol et al., 1994). The amount of CO<sub>2</sub> for each RPO fraction, their δ<sup>13</sup>C values, and Fm (framction modern) values were corrected for blank carbon contributions from the RPO system (resulting in corrections of no more than 3 ‰ for any sample). δ<sup>13</sup>C was additionally corrected to ensure <sup>13</sup>C mass balance.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736656 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736656
completed
Margot Elizabeth White
University of California-San Diego Scripps
mew070@ucsd.edu
pointOfContact
Lihini Aluwihare
University of California-San Diego Scripps
858-822-4886
9500 Gilman Drive
La Jolla
CA
92093-0244
USA
laluwihare@ucsd.edu
pointOfContact
Steven R. Beaupré
Stony Brook University - SoMAS
631-632-3748
123 Challenger Hall School of Marine and Atmospheric Sciences
Stony Brook
NY
11794
USA
steven.beaupre@stonybrook.edu
pointOfContact
John Michael Beman
University of California-Merced
650-823-7925
5200 North Lake Road
Merced
CA
95343
USA
jmbeman@gmail.com
pointOfContact
Ann McNichol
Woods Hole Oceanographic Institution
amcnichol@whoi.edu
pointOfContact
Kenneth Smith
Monterey Bay Aquarium Research Institute
(831) 775-1710
7700 Sandholdt Road MBARI
Moss Landing
CA
95039
USA
ksmith@mbari.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Latitude
Longitude
Depth
Sample_Type
Ramp_Rate
Fraction
Start_Temp
End_Temp
Carbon_Mass_ug
d13C
C14
National Ocean Sciences Accelerator Mass Spectrometer
drying oven
tube furnace
nondispersive infrared CO2 detector
Dual inlet IRMS
mass flow controller
theme
None, User defined
latitude
longitude
depth
sample type
No BCO-DMO term
sample identification
temperature of sample or a generic measurement (not in-situ water temp)
Elemental carbon (C)
d13C
14C
featureType
BCO-DMO Standard Parameters
Accelerator Mass Spectrometer
Drying Oven
furnace
Gas Analyzer
Isotope-ratio Mass Spectrometer
Mass Flow Controller
instrument
BCO-DMO Standard Instruments
Benthic-Pelagic Coupling 2018 expedition
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Investigating the contribution of carotenoid degradation products to refractory dissolved organic matter (DOM)
https://osprey.bco-dmo.org/project/876814
Investigating the contribution of carotenoid degradation products to refractory dissolved organic matter (DOM)
<p><strong>NSF Award Abstract:</strong></p>
<p>This research project will identify biological sources and chemical structures that are responsible for the long-term storage of carbon in the ocean. Each year, microscopic marine plants remove about as much carbon dioxide from the atmosphere as do land plants. Respiration returns much of this carbon to the ocean as carbon dioxide, but some is locked in the remnants of living organisms. These remaining compounds are modified by pathways that involve bacteria, sunlight, chemical reactions, and other processes that lead to storage of carbon for thousands to millions of years. Some compounds eventually contribute to the petroleum reservoir. Building on previous results, this project will study the reactions and oceanic lifetime of a particular set of biochemicals, called carotenoids, as a possible organic carbon storage pathway. Carotenoids are abundant in very many marine organisms, increasing the likelihood that they are part of this long-term carbon storage and petroleum formation. These compounds also have unique chemical properties that make them subject to specific chemical reactions. For this reason, they have been marketed as powerful antioxidants. Therefore, scientific outcomes from this research on carotenoid chemistry will not only inform ocean carbon cycles but could also benefit studies of their properties as antioxidants. The project will determine the lifetime of carotenoids and their degradation products in seawater to provide new insights into pathways that transfer carbon from the atmosphere through biota and into long-term storage reservoirs. Graduate students and underrepresented undergraduate students will be engaged in the research.</p>
<p>Previous work has identified specific chemical backbones of compounds that are broadly distributed within the marine dissolved organic matter (DOM) reservoir. A high-resolution analytical approach that combines nuclear magnetic resonance (NMR) spectroscopy with comprehensive gas chromatography-mass spectrometry (GC-MS) has detected DOM compounds with unique structures closely related to carotenoids. Photochemical reactions of a representative carotenoid in laboratory experiments has further linked compounds detected in seawater to carotenoid degradation products (CDP). These preliminary studies show promise that the work funded here will be able to identify specific CDP structures and establish the quantitative significance, lifetimes, and timescales of CDP accumulation in seawater. The project will combine laboratory experiments, high resolution analyses, and chemical synthesis methods to determine the chemical composition of CDP and their abundance in seawater. The novel analytical methods developed through this research will be relevant for other carotenoid-focused studies in petroleum formation, soil chemistry, as well as food chemistry. Ramped pyrolysis oxidation (PyrOx) coupled to radiocarbon measurements will be used to determine the radiocarbon content of CDP-enriched DOM and seek to estimate the accumulation timescale of these dissolved molecules in the environment, and it is hypothesized that deeper, older ocean water will contain a relatively higher proportion of radiocarbon-depleted CDP. Collecting samples from different depths in the North Pacific Ocean where CDP-enriched DOM will be isolated following established sample processing methods will provide insights and new information on the mechanisms that control the amount and timescale of carbon redistribution among Earth's various reservoirs.</p>
<p><strong>Resources:</strong><br />
Ramped pyrolysis oxidation (RPO) data is currently accessible through the RPO database maintained on GitHub by Jordon Hemingway (<a href="http://github.com/FluvialSeds/RPO_Database">http://github.com/FluvialSeds/RPO_Database</a>).</p>
<p>J.D. Hemingway et al. A compiled database of published ramped pyrolysis/oxidation results, 2018- , <a href="http://pypi.python.org/pypi/RPO_Database">http://pypi.python.org/pypi/RPO_Database</a>, doi:10.5281/zenodo.1158742 [online; accessed 2022-07-10]</p>
<p> </p>
<p> </p>
CDP in rDOM
largerWorkCitation
project
eng; USA
oceans
-123
-123
34.833333
34.833333
2018-04-19
2018-04-23
Eastern North Pacific Ocean
0
BCO-DMO catalogue of parameters from Ramped pyrolysis oxidation (RPO) carbon isotope data from DOC in water samples collected during R/V Western Flyer Benthic-Pelagic Coupling expedition to Station M in the eastern Pacific Ocean in April 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/892893.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/892894.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of samples collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/892895.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/892896.rdf
Name: Sample_Type
Units: unitless
Description: <p>Type of sample undergoing RPO. PPL DOM = Priority PolLutant DOM obtained from solid phase exraction; Hydrolyzed PPL DOM = PPL DOM hydrolyzed with 2M hydrochloric acid and re-extracted onto PPL resin</p>
http://lod.bco-dmo.org/id/dataset-parameter/892897.rdf
Name: Ramp_Rate
Units: degrees Celsius per min (°C/min)
Description: <p>Rate of increase of temperature during thermal oxidation</p>
http://lod.bco-dmo.org/id/dataset-parameter/892898.rdf
Name: Fraction
Units: unitless
Description: <p>Number assigned to sample of CO2 collected for isotopic measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/892899.rdf
Name: Start_Temp
Units: degrees Celsius
Description: <p>Temperature at start of sample collection for CO2 fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/892900.rdf
Name: End_Temp
Units: degrees Celsius
Description: <p>Temperature at end of sample collection for CO2 fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/892901.rdf
Name: Carbon_Mass_ug
Units: micrograms (ug)
Description: <p>Mass of carbon in CO2 sample collected for isotopic measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/892902.rdf
Name: d13C
Units: per mil
Description: <p>Stable carbon (13C) isotopic signature of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/892903.rdf
Name: C14
Units: units
Description: <p>Radiocarbon (14C) signature of sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3022
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/f9a101bb-352e-4223-9b3b-5762fcc54909/content
download
https://doi.org/10.26008/1912/bco-dmo.892660.1
download
onLine
dataset
<p><strong>Field collection of samples</strong><br />
Samples were collected during a cruise aboard the R/V Western Flyer to Station M off the coast of central California in April 2018 (34°<sup>&nbsp;</sup>50’N, 123°<sup>&nbsp;</sup>00’W; 4100 meters depth).&nbsp; 30 to 40 Liters of seawater were collected at five depths ranging from 45 to 3700 meters.&nbsp;Water was then acidified to pH 2&nbsp;using trace metal grade hydrochloric acid (HCl). Solid phase extraction columns (1g Bond Elut PPL cartridges by Agilent) were first activated overnight with liquid chromatography/mass spectrometry (LC/MS) grade methanol and then rinsed with LC/MS grade water, LC/MS grade methanol, and LC/MS grade water adjusted to pH 2 using trace metal grade HCl. The acidified seawater was passed through the PPL cartridges under gravitational pressure over 1-2 days. This PPL solid phase extraction method&nbsp;has been used extensively to isolate marine DOM (Dittmar et al., 2008; Petras et al., 2017 and more) and provides yields of 35-60%. Following acidification, the method preferentially extracts hydrophobic compounds but is also able to isolate some semi-polar compounds (Johnson et al., 2017; Petras et al., 2021) whose overall concentrations may change with depth. In addition to the relatively high yield, previous work has shown that Δ<sup>14</sup>C of PPL-DOC is similar to that of the bulk (Lechtenfeld et al., 2014; Lewis et al., 2021). Typically, 5L of seawater was extracted with one 1g PPL cartridge.</p>
<p>Before eluting the PPL resin, and immediately following the extraction, the cartridge was rinsed with three column volumes of pH 2 LC/MS grade water and dried under ultra-high purity nitrogen gas. Organic matter retained on the cartridge was then eluted with 10 mL of methanol/1 g PPL cartridge (PPL-DOC) and stored at -20 °C. Seawater for total organic carbon concentration [TOC] and dissolved organic carbon concentration [DOC] analysis (i.e., seawater pre-filtered through combusted Whatman corporation GF/F filters)&nbsp;was collected into combusted 40-mL borosilicate vials, immediately acidified to pH 2 using trace metal grade 12 N HCl (Fisher Scientific), capped with acid-washed vial caps with septa, and stored at room temperature until measurement.&nbsp;</p>
<p>A fraction of the extracted PPL-DOC sample was dried extensively to remove methanol and then subjected to a 2 M acid hydrolysis. Briefly, the organic matter was transferred to an ampoule with 2 mL of 2 M hydrochloric acid and sealed under a nitrogen atmosphere before being placed in a drying&nbsp;oven at 80°C for 18 hours. Following hydrolysis, the sample was diluted with LC/MS grade water to pH 2 and then re-extracted onto a PPL cartridge as described above. The organic matter retained by the cartridge was then eluted in methanol and dried down for frozen storage.&nbsp;</p>
<p><strong>Laboratory sampling</strong><br />
Ramped pyrolysis/oxidation (RPO) was performed at the National Ocean Sciences Accelerator Mass Spectrometry (NOSAMS) facility in Woods Hole, MA, USA. Samples were transferred while dissolved in a small amount of methanol into quartz cups (pre-combusted at 850 °C, 1 hour) and then dried down under ultra-high purity nitrogen gas. Samples were further dried in an oven at 50°C for multiple days in an attempt to remove all methanol. Each quartz cup containing a single sample was then placed inside the RPO reactor via a quartz insert tube. The RPO instrument continuously heats the sample at a set rate, monitors evolved CO<sub>2&nbsp;</sub>during the pyrolysis/oxidation reactions as “thermograms” (Rosenheim et al., 2008), and can be used to trap CO<sub>2&nbsp;</sub>for isotope analysis. (For Station M thermogram data, see BCO-DMO dataset here: https://www.bco-dmo.org/dataset/892564).&nbsp;The RPO methodology has been described previously and further information describing the detailed protocol can be found in other publications (Hemingway et al., 2017; Rosenheim et al., 2008). In summary, all samples were operated in oxidation mode (carrier gas 92% He, 8% O<sub>2</sub>) with a flow rate of 35 mL min<sup>−1</sup>&nbsp;and a ramp rate of either 20 or 5 degrees Celsius per minute. Samples analyzed at 20°C min<sup>−1&nbsp;</sup>ranged in size from 6.4 to 36.8 µmol C and all resulting CO<sub>2</sub> was collected for isotope analysis. Samples analyzed at 5°C min<sup>−1</sup>&nbsp;ranged in size from 48.2 to 185.2 µmol C, where these amounts represent the sums of six fractions collected during each 5°C min<sup>−1</sup>&nbsp;ramp, with each fraction ranging in size from 4.4 to 43.6 µmol C.&nbsp; In total we ran ten 20°C min<sup>−1</sup>&nbsp;ramps and six 5°C min<sup>−1</sup>&nbsp;ramps of samples from Station M.</p>
<p><strong>Isotope measurements</strong><br />
All bulk and RPO-fraction isotope measurements were performed at NOSAMS. Stable carbon isotope compositions were measured on the resultant CO<sub>2</sub> gas using a dual-inlet isotope ratio mass spectrometer (IRMS), with resulting <sup>13</sup>C content expressed in δ<sup>13</sup>C per mil (‰) notation relative to Vienna Pee Dee Belemnite (VPDB). Radiocarbon measurements were completed via accelerator mass spectrometry (AMS) following standard graphitization methods (McNichol et al., 1994). The amount of CO<sub>2</sub> for each RPO fraction, their δ<sup>13</sup>C values, and Fm (framction modern) values were corrected for blank carbon contributions from the RPO system (resulting in corrections of no more than 3 ‰ for any sample). δ<sup>13</sup>C was additionally corrected to ensure <sup>13</sup>C mass balance.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p>The amount of CO<sub>2</sub>&nbsp;for each RPO fraction, their δ<sup>13</sup>C values, and Fm values&nbsp;were corrected for blank carbon contributions from the RPO system following&nbsp;Hemingway et al. 2017a (resulting in corrections of no more than 3‰ for any sample). δ<sup>13</sup>C was additionally corrected to ensure&nbsp;<sup>13</sup>C mass balance, using the ‘ramped pyrox’ python package developed by Hemingway et al., 2017b and available online at&nbsp;http://pypi.python.org/pypi/rampedpyrox.</p>
<p><strong>Problems/Issues</strong><br />
Residual methanol may be contributing to the first fraction collected for some of the samples when this fraction was small (~50&nbsp;µg). This would result in a lower Δ<sup>14</sup>C signature for the first fraction, potentially masking the presence of younger carbon. This contamination would not have affected the bulk isotope measurements which were made on larger samples, and which did not have anomalous δ<sup>13</sup>C values. The potential for residual methanol to linger even after several drying steps, should be kept in mind when interpreting unexpectedly depleted δ<sup>13</sup>C and Δ<sup>14</sup>C values for PPL DOC samples.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
National Ocean Sciences Accelerator Mass Spectrometer
National Ocean Sciences Accelerator Mass Spectrometer
PI Supplied Instrument Name: National Ocean Sciences Accelerator Mass Spectrometer PI Supplied Instrument Description:All bulk and RPO-fraction isotope measurements were performed at NOSAMS. Radiocarbon measurements were completed via accelerator mass spectrometry (AMS) following standard graphitization methods Instrument Name: Accelerator Mass Spectrometer Instrument Short Name:AMS Instrument Description: An AMS measures "long-lived radionuclides that occur naturally in our environment. AMS uses a particle accelerator in conjunction with ion sources, large magnets, and detectors to separate out interferences and count single atoms in the presence of 1x1015 (a thousand million million) stable atoms, measuring the mass-to-charge ratio of the products of sample molecule disassociation, atom ionization and ion acceleration." AMS permits ultra low-level measurement of compound concentrations and isotope ratios that traditional alpha-spectrometry cannot provide. More from Purdue University: http://www.physics.purdue.edu/primelab/introduction/ams.html Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB17/
drying oven
drying oven
PI Supplied Instrument Name: drying oven PI Supplied Instrument Description:The samples were placed in a drying oven at 80°C for 18 hours. Instrument Name: Drying Oven Instrument Short Name: Instrument Description: a heated chamber for drying
tube furnace
tube furnace
PI Supplied Instrument Name: tube furnace PI Supplied Instrument Description:As part of the ramped oxidation pyrolysis (RPO) setup, tube furnaces with independent temperature controllers were used for both combustion and pyrolysis. Instrument Name: furnace Instrument Short Name: Instrument Description: An enclosed chamber designed to produce heat.
nondispersive infrared CO2 detector
nondispersive infrared CO2 detector
PI Supplied Instrument Name: nondispersive infrared CO2 detector PI Supplied Instrument Description:Evolved carbon dioxide (CO2) was measured by a flow-through infrared gas analyzer and thermograms were constructed by plotting gas concentrations over time. Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
Dual inlet IRMS
Dual inlet IRMS
PI Supplied Instrument Name: Dual inlet IRMS PI Supplied Instrument Description:All bulk and RPO-fraction isotope measurements were performed at NOSAMS. Stable carbon isotope compositions were measured on the resultant CO2 gas using a dual-inlet isotope ratio mass spectrometer (IRMS). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
mass flow controller
mass flow controller
PI Supplied Instrument Name: mass flow controller PI Supplied Instrument Description:The RPO system consists of a flow system (with mass flow controllers and infrared gas analyzer), a furnace system, and a vacuum separations system. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
Cruise: Benthic-Pelagic Coupling 2018 expedition
Benthic-Pelagic Coupling 2018 expedition
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
Benthic-Pelagic Coupling 2018 expedition
Kenneth Smith
Monterey Bay Aquarium Research Institute
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel