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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/892997.rdf" xlink:actuate="onRequest">Nutrient concentrations and stable isotope values for groundwater in nearshore shelf waters off Charleston, SC from June 2018 to August 2019</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Pinckney, J. L. (2023) Nutrient concentrations and stable isotope values for groundwater in nearshore shelf waters off Charleston, SC from June 2018 to August 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.892997.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Nutrient concentrations and stable isotope values for groundwater were measured on selected dates from 7 June 2018 to 10 August 2019 in nearshore shelf waters off Charleston, SC (32° 42’ N, 79° 50’ W and 32° 51’ N, 79° 09’ W). At each of the groundwater wells in the wellfield, divers collected fluids from the wells to determine well water inorganic nutrient concentrations (nitrate + nitrite, ammonium, orthophosphate) and the δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of well water DON, ammonium, and nitrate (when present). Sediment core samples were analyzed by HPLC. These data were used to map nutrient concentrations and assess inputs of groundwater effects on benthic microalgae.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of Nitrate:&amp;lt;/strong&amp;gt; Filtered (0.2 micrometer) samples were stored in acid-washed HDPE bottles and frozen (-20 degrees Celsius) until analysis. For any sample with more than 0.5 μM NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;, the isotopic composition of NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;- &amp;lt;/sup&amp;gt;was determined by the denitrifier method of Sigman et al. (2001) and Casciotti et al. (2002), with updates according to McIlvin &amp;amp;amp; Casciotti (2011) and Weigand et al. (2016).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of Ammonium:&amp;lt;/strong&amp;gt; Filtered (0.2 μm) samples will be stored on dry ice in the field and shipped immediately to the lab for δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of ammonium analysis. Samples with more than 3 µM NH&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; and adequate sample volume were analyzed using the hypobromite/azide method of Zhang et al. (2007).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/712749.rdf" xlink:title="OCE-1736557" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736557 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736557</gmx:Anchor>
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Continental shelves are highly productive, with both ecological and economic importance. Benthic microalgae (BMA) are key primary producers in these location. As much as 6x the water column biomass of primary producers is compressed into a layer only a few mm thick on the sediment surface. The source(s) of fixed nitrogen (N) supporting such highly concentrated BMA biomass is currently unknown. Recent studies of sub-seafloor groundwater flow at the University of South Carolina have demonstrated that upwelling saline groundwater likely supplies high concentrations of nutrients in the ridge-swale habitats in the South Atlantic Bight (SAB). The investigators suggest that groundwater input of fixed N into surficial sediments is the primary source of N supporting BMA biomass and production in the mid-shelf region of the SAB. The purpose of this project is to determine the primary source of fixed N supporting BMA biomass in the surface sediments of the shallow shelf waters (&amp;lt;30 m), using the SAB as a field area. A secondary objective is to apply novel and innovative methods to directly quantify groundwater inputs of N into surficial sediments. Research results will fully document the spatio-temporal distributions of BMA and phytoplankton biomass and community structure in the mid-shelf region of the SAB and relate the observed patterns to groundwater inputs of fixed N sources as well as hydrographic and climatic conditions. This research will provide full support and tuition for 2 graduate students, summer support for undergraduate assistants, and involve upper level undergraduates as lab interns. The study team will also work with the Baruch Institute and other partners to develop an &quot;Ocean Schoolyard&quot; program to meet the needs of teachers, students, and community audiences. The project will also provide partial support for Girls Go for I.T., a coding summer camp designed to attract middle-school-aged girls to careers in I.T. and STEM fields.&lt;/p&gt;
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                <gco:CharacterString>&amp;lt;p&amp;gt;Nutrient concentrations and stable isotope values for groundwater were measured on selected dates from 7 June 2018 to 10 August 2019 in nearshore shelf waters off Charleston, SC (32° 42’ N, 79° 50’ W and 32° 51’ N, 79° 09’ W). At each of the groundwater wells in the wellfield, divers collected fluids from the wells to determine well water inorganic nutrient concentrations (nitrate + nitrite, ammonium, orthophosphate) and the δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of well water DON, ammonium, and nitrate (when present). Sediment core samples were analyzed by HPLC. These data were used to map nutrient concentrations and assess inputs of groundwater effects on benthic microalgae.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of Nitrate:&amp;lt;/strong&amp;gt; Filtered (0.2 micrometer) samples were stored in acid-washed HDPE bottles and frozen (-20 degrees Celsius) until analysis. For any sample with more than 0.5 μM NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;, the isotopic composition of NO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;- &amp;lt;/sup&amp;gt;was determined by the denitrifier method of Sigman et al. (2001) and Casciotti et al. (2002), with updates according to McIlvin &amp;amp;amp; Casciotti (2011) and Weigand et al. (2016).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of Ammonium:&amp;lt;/strong&amp;gt; Filtered (0.2 μm) samples will be stored on dry ice in the field and shipped immediately to the lab for δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N of ammonium analysis. Samples with more than 3 µM NH&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; and adequate sample volume were analyzed using the hypobromite/azide method of Zhang et al. (2007).&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/484.rdf" xlink:title="Fluorometer" xlink:actuate="onRequest">Turner Trilogy fluorometer</gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name: Turner Trilogy fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer   Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/685.rdf" xlink:title="Mass Spectrometer" xlink:actuate="onRequest">Thermo Finnigan DeltamV isotope ratio mass spectrometer</gmx:Anchor>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/713363.rdf" xlink:title="Self-Contained Underwater Breathing Apparatus" xlink:actuate="onRequest"></gmx:Anchor>
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Reference: https://oceanexplorer.noaa.gov/technology/technical/technical.html</gco:CharacterString>
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        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/595.rdf" xlink:title="UV Spectrophotometer-Shimadzu" xlink:actuate="onRequest">Shimadzu UV-1800 uv-vis spectrophotometer</gmx:Anchor>
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