http://lod.bco-dmo.org/id/dataset/893487
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-04-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Total dissolved cobalt and labile dissolved cobalt distributions measured by shipboard voltammetry in the Amundsen Sea, Ross Sea, and Terra Nova Bay during the CICLOPS expedition on RVIB Nathaniel B. Palmer (NBP1801) from Dec 2017 to Feb 2018
2023-04-11
publication
2023-04-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-04-13
publication
https://doi.org/10.26008/1912/bco-dmo.893487.1
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saito, M. A. (2023) Total dissolved cobalt and labile dissolved cobalt distributions measured by shipboard voltammetry in the Amundsen Sea, Ross Sea, and Terra Nova Bay during the CICLOPS expedition on RVIB Nathaniel B. Palmer (NBP1801) from Dec 2017 to Feb 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.893487.1 [access date]
CICLOPS Dissolved Cobalt Dataset Description: Methods and Sampling: <p>Samples were collected along the coastal Antarctic Shelf from the Amundsen Sea, Ross Sea, and Terra Nova Bay during the CICLOPS expedition on the RVIB Nathanial B. Palmer (cruise ID NBP-1801) from December 11, 2017 to March 3, 2018. Dissolved seawater was collected from full-depth station profiles using a trace metal clean sampling rosette deployed on a conducting synthetic line, both supplied by the U.S. Antarctic Program (USAP), and equipped with twelve 8-liter (L) X-Niskin bottles (Ocean Test Equipment), supplied by the Saito Laboratory. Real-time trace metal rosette operations allowed for the careful collection of seawater from 10 and 20 meters (m) above the ocean floor to study sediment-water interactions within a potential nepheloid layer. After deployment, the X-Niskin bottles were transported to a trace metal clean van and pressurized with high-purity N₂ gas. Seawater samples for dCo analysis were then filtered through acid-washed 0.2-micromole (µM) Supor polyethersulfone membrane filters (Pall Corporation, 142-millimeter (mm) diameter) within 3 hours of rosette recovery.</p>
<p>To minimize metal contamination of samples, all sample bottles were prepared using trace metal clean procedures, including soaking sample bottles for ~1 week in Citranox, an acidic detergent, rinsing with Milli-Q water (Millipore), soaking sample bottles for ~2 weeks in 10% trace metal grade HCl (Optima, Fisher Scientific), and rinsing with lightly acidic Milli-Q water (&gt; 0.1% HCl).</p>
<p>Samples for dCo analysis were collected in 60-milliliter (mL) low-density polyethylene (LDPE) bottles and stored at 4⁰C until analysis. Duplicate dCo samples were collected: one for at-sea analysis of labile dCo and total dCo, and another for preservation and total dCo analysis in the laboratory after the expedition. Preserved total dCo samples were stored with oxygen-absorbing satchels (Mitsubishi Gas Chemical, model RP-3K), which preserve the sample for long-term storage and future analysis (Noble et al. 2017; Bundy et al. 2020). Preserved dCo samples were stored in groups of 6 within an open (unsealed) plastic bag, which was then placed into a gas-impermeable plastic bag (Ampac) with one oxygen-absorbing satchel per 60 mL dCo sample. The outer bag was then heat-sealed and stored at 4°C until analysis.</p>
<p>Total dCo – the combined fractions of labile and ligand-bound dCo, hereafter simply dCo – and labile dCo concentrations were analyzed via cathodic stripping voltammetry (CSV) as described by Saito and Moffett (2001) and modified by Saito et al. (2010) and Hawco et al. (2016). CSV analysis was conducted using a Metrohm 663 VA and µAutolabIII systems equipped with a hanging mercury drop working electrode. All reagents were prepared as described in Chmiel et al. (2022). Most samples were analyzed at sea within 3 weeks of sample collection, and stations 57 and 60 were analyzed for labile dCo at sea and their duplicate preserved samples were analyzed for total dCo in November 2019 in the laboratory.</p>
<p>To measure total dCo concentrations, filtered seawater samples were first UV-irradiated in quartz tubes for one hour in a Metrohm 705 UV Digester to destroy natural ligand-bound Co complexes. 11 mL of sample was then added to a 15 mL trace metal clean polypropylene vial, and 100 microliters (µL) of 0.1 molar (M) dimethyglyoxime (DMG; Sigma Aldrich) ligand and 130 µL of 0.5 M N-(2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (EPPS, Sigma Aldrich) buffer was added to each sample vial. A Metrohm 858 Sample Processor then loaded 8.5 mL of each sample into the electrode's Teflon cup and added 1.5 mL of 1.5 M NaNO₂ reagent (Merck). The mercury electrode performed a fast linear sweep from -1.4 volts (V) to -0.6 V at a rate of 5 volts per second (V s⁻¹) and produced a cobalt reduction peak at -1.15 V, the voltage at which the Co(DMG)₂ complex is reduced from Co(II) to Co(0) (Saito and Moffett 2001). The height of the Co reduction peak is linearly proportional to the amount of total dCo present in the sample. Peak heights were determined by NOVA 1.10 software. A standard curve was created with 4 additions of 25 picomoles(pM) dCo to each sample, and a type-I linear regression of the addition standard curve performed by the LINEST function in Microsoft Excel allowed for the calculation of the initial amount of Co present in the sample.</p>
<p>When analyzing labile dCo concentrations, samples were not UV-irradiated so as to only quantify the free or weakly bound dCo not bound to strong organic ligands. 11 mL of labile samples were instead allowed to equilibrate with the DMG ligand and EPPS reagent overnight (~8 hours) before analysis so as to allow time for the labile dCo present in the sample to bind to the DMG ligand via competitive ligand exchange (K &gt; 10^16.8). Labile dCo samples were then loaded onto the Sample Processor and analyzed electrochemically using identical methods as described above for total dCo samples.</p>
<p><strong>Known Issues/Problems:</strong><br />
Many of the dCo and labile dCo values measured were unusually low and below the analytical detection limit of 4 pM. In cases where no dCo or labile dCo were detected (i.e. when no peak was measurable and/or the dCo value predicted was &lt; 0 pM), values of 0 pM were assigned for the purposes of plotting and select statistical analysis and were flagged as not detected (n.d.) as well as &lt;DL in the dataset; although these concentrations are not detectable with our methodology, we believe the incredibly low concentrations of dCo and labile dCo observed on this expedition were meaningful, and that removing these values from our analysis misrepresents the data and would skew the results to appear higher than was observed.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1644073 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1644073
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643684 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643684
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643845 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643845
completed
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Longitude
Latitude
Bottle_Number
Depth_m
Bottom_Depth_m
ISO_DateTime_UTC
Temperature
Conductivity
dO2_mg_L
Fluorescence
Percent_Transmisson
PAR
Salinity
PO4_uM
NO3_NO2_uM
Silicate_uM
NO2_uM
NH4_uM
dCo_pM
dCo_Flag
Labile_dCo_pM
Labile_dCo_Flag
Metrohm 858 Sample Processor
Metrohm 663 VA
X-Niskin
Metrohm 705 UV Digester
µAutolabIII
theme
None, User defined
station
longitude
latitude
bottle
depth
depth_bottom
ISO_DateTime_UTC
water temperature at measurement depth
salinity
dissolved Oxygen
fluorescence
transmission
PAR
reactive phosphorus (PO4)
nitrate plus nitrite
Silicate, Si(OH)4, silicic acid
Nitrite
Ammonium
Cobalt
quality flag
featureType
BCO-DMO Standard Parameters
Laboratory Autosampler
Metrohm 663 VA Stand mercury electrode
Niskin bottle
UV Digester
Voltammetry Analyzers
instrument
BCO-DMO Standard Instruments
NBP1801
service
Deployment Activity
Western Ross Sea, Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
https://osprey.bco-dmo.org/project/774945
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
<p>NSF abstract:<br />
Phytoplankton blooms in the coastal waters of the Ross Sea, Antarctica are typically dominated by either diatoms or Phaeocystis Antarctica (a flagellated algae that often can form large colonies in a gelatinous matrix). The project seeks to determine if an association of bacterial populations with Phaeocystis antarctica colonies can directly supply Phaeocystis with Vitamin B12, which can be an important co-limiting micronutrient in the Ross Sea. The supply of an essential vitamin coupled with the ability to grow at lower iron concentrations may put Phaeocystis at a competitive advantage over diatoms. Because Phaeocystis cells can fix more carbon than diatoms and Phaeocystis are not grazed as efficiently as diatoms, the project will help in refining understanding of carbon dynamics in the region as well as the basis of the food web webs. Such understanding also has the potential to help refine predictive ecological models for the region. The project will conduct public outreach activities and will contribute to undergraduate and graduate research. Engagement of underrepresented students will occur during summer student internships. A collaboration with Italian Antarctic researchers, who have been studying the Terra Nova Bay ecosystem since the 1980s, aims to enhance the project and promote international scientific collaborations.</p>
<p>The study will test whether a mutualistic symbioses between attached bacteria and Phaeocystis provides colonial cells a mechanism for alleviating chronic Vitamin B12 co-limitation effects thereby conferring them with a competitive advantage over diatom communities. The use of drifters in a time series study will provide the opportunity to track in both space and time a developing algal bloom in Terra Nova Bay and to determine community structure and the physiological nutrient status of microbial populations. A combination of flow cytometry, proteomics, metatranscriptomics, radioisotopic and stable isotopic labeling experiments will determine carbon and nutrient uptake rates and the role of bacteria in mitigating potential vitamin B12 and iron limitation. Membrane inlet and proton transfer reaction mass spectrometry will also be used to estimate net community production and release of volatile organic carbon compounds that are climatically active. Understanding how environmental parameters can influence microbial community dynamics in Antarctic coastal waters will advance an understanding of how changes in ocean stratification and chemistry could impact the biogeochemistry and food web dynamics of Southern Ocean ecosystems.</p>
CICLOPS
largerWorkCitation
project
eng; USA
oceans
Western Ross Sea, Southern Ocean
179.819
-151.918
-76.7499
-72.7507
2017-12-30
2018-02-08
Amundsen Sea, Ross Sea, Terra Nova Bay
0
BCO-DMO catalogue of parameters from Total dissolved cobalt and labile dissolved cobalt distributions measured by shipboard voltammetry in the Amundsen Sea, Ross Sea, and Terra Nova Bay during the CICLOPS expedition on RVIB Nathaniel B. Palmer (NBP1801) from Dec 2017 to Feb 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/893583.rdf
Name: Station
Units: unitless
Description: <p>Station ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/893584.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Longitude of sampling location (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/893585.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Latitude of sampling location (South is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/893586.rdf
Name: Bottle_Number
Units: unitless
Description: <p>Trace metal rosette niskin bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/893587.rdf
Name: Depth_m
Units: meters (m)
Description: <p>Depth of trace metal rosette when sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/893588.rdf
Name: Bottom_Depth_m
Units: meters (m)
Description: <p>Station bottom seafloor depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/893589.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Trace metal rosette start date and time in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/893590.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Temperature of seawater from trace metal CTD temperature sensor</p>
http://lod.bco-dmo.org/id/dataset-parameter/893591.rdf
Name: Conductivity
Units: millisiemens per centimeter (mS/cm)
Description: <p>Conductivity of seawater from trace metal CTD conductivity sensor</p>
http://lod.bco-dmo.org/id/dataset-parameter/893592.rdf
Name: dO2_mg_L
Units: milligrams per liter (mg/L)
Description: <p>Concentration of dissolved oxygen from trace metal CTD oxygen sensor</p>
http://lod.bco-dmo.org/id/dataset-parameter/893593.rdf
Name: Fluorescence
Units: milligrams per cubic meter (mg/m^3)
Description: <p>Fluorescence of seawater from trace metal CTD fluorometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/893594.rdf
Name: Percent_Transmisson
Units: unitless (percent)
Description: <p>Beam Transmission from trace metal CTD Transmissometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/893595.rdf
Name: PAR
Units: watts per square meter (W/m^2)
Description: <p>Photosynthetically Active Radiation (PAR) from trace metal CTD PAR sensor</p>
http://lod.bco-dmo.org/id/dataset-parameter/893596.rdf
Name: Salinity
Units: PSU
Description: <p>Salinity derived from conductivity measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/893597.rdf
Name: PO4_uM
Units: micromolar (uM)
Description: <p>Concentration of dissolved phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/893598.rdf
Name: NO3_NO2_uM
Units: micromolar (uM)
Description: <p>Concentration of dissolved nitrate plus dissolved nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/893599.rdf
Name: Silicate_uM
Units: micromolar (uM)
Description: <p>Concentration of dissolved silicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/893600.rdf
Name: NO2_uM
Units: micromolar (uM)
Description: <p>Concentration of dissolved nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/893601.rdf
Name: NH4_uM
Units: micromolar (uM)
Description: <p>Concentration of dissolved ammonia</p>
http://lod.bco-dmo.org/id/dataset-parameter/893602.rdf
Name: dCo_pM
Units: picomolar (pM)
Description: <p>Concentration of total dissolved cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/893603.rdf
Name: dCo_Flag
Units: unitless
Description: <p>Quality flags for total dissolved cobalt measurement. When detectable dCo concentrations were found below the 4 pM detection limit, their values were preserved in the dataset and flagged as below the detection limit (<DL).</p>
http://lod.bco-dmo.org/id/dataset-parameter/893604.rdf
Name: Labile_dCo_pM
Units: picomolar (pM)
Description: <p>Concentration of labile dissolved cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/893605.rdf
Name: Labile_dCo_Flag
Units: unitless
Description: <p>Quality flags for labile dissolved cobalt measurement. When detectable dCo concentrations were found below the 4 pM detection limit, their values were preserved in the dataset and flagged as below the detection limit (<DL).</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
22769
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/2e2da8b9-597e-4f77-954e-eeca3372360e/content
download
https://doi.org/10.26008/1912/bco-dmo.893487.1
download
onLine
dataset
<p>Samples were collected along the coastal Antarctic Shelf from the Amundsen Sea, Ross Sea, and Terra Nova Bay during the CICLOPS expedition on the RVIB Nathanial B. Palmer (cruise ID NBP-1801) from December 11, 2017 to March 3, 2018. Dissolved seawater was collected from full-depth station profiles using a trace metal clean sampling rosette deployed on a conducting synthetic line, both supplied by the U.S. Antarctic Program (USAP), and equipped with twelve 8-liter (L) X-Niskin bottles (Ocean Test Equipment), supplied by the Saito Laboratory. Real-time trace metal rosette operations allowed for the careful collection of seawater from 10 and 20 meters (m) above the ocean floor to study sediment-water interactions within a potential nepheloid layer. After deployment, the X-Niskin bottles were transported to a trace metal clean van and pressurized with high-purity N₂ gas. Seawater samples for dCo analysis were then filtered through acid-washed 0.2-micromole (µM) Supor polyethersulfone membrane filters (Pall Corporation, 142-millimeter (mm) diameter) within 3 hours of rosette recovery.</p>
<p>To minimize metal contamination of samples, all sample bottles were prepared using trace metal clean procedures, including soaking sample bottles for ~1 week in Citranox, an acidic detergent, rinsing with Milli-Q water (Millipore), soaking sample bottles for ~2 weeks in 10% trace metal grade HCl (Optima, Fisher Scientific), and rinsing with lightly acidic Milli-Q water (&gt; 0.1% HCl).</p>
<p>Samples for dCo analysis were collected in 60-milliliter (mL) low-density polyethylene (LDPE) bottles and stored at 4⁰C until analysis. Duplicate dCo samples were collected: one for at-sea analysis of labile dCo and total dCo, and another for preservation and total dCo analysis in the laboratory after the expedition. Preserved total dCo samples were stored with oxygen-absorbing satchels (Mitsubishi Gas Chemical, model RP-3K), which preserve the sample for long-term storage and future analysis (Noble et al. 2017; Bundy et al. 2020). Preserved dCo samples were stored in groups of 6 within an open (unsealed) plastic bag, which was then placed into a gas-impermeable plastic bag (Ampac) with one oxygen-absorbing satchel per 60 mL dCo sample. The outer bag was then heat-sealed and stored at 4°C until analysis.</p>
<p>Total dCo – the combined fractions of labile and ligand-bound dCo, hereafter simply dCo – and labile dCo concentrations were analyzed via cathodic stripping voltammetry (CSV) as described by Saito and Moffett (2001) and modified by Saito et al. (2010) and Hawco et al. (2016). CSV analysis was conducted using a Metrohm 663 VA and µAutolabIII systems equipped with a hanging mercury drop working electrode. All reagents were prepared as described in Chmiel et al. (2022). Most samples were analyzed at sea within 3 weeks of sample collection, and stations 57 and 60 were analyzed for labile dCo at sea and their duplicate preserved samples were analyzed for total dCo in November 2019 in the laboratory.</p>
<p>To measure total dCo concentrations, filtered seawater samples were first UV-irradiated in quartz tubes for one hour in a Metrohm 705 UV Digester to destroy natural ligand-bound Co complexes. 11 mL of sample was then added to a 15 mL trace metal clean polypropylene vial, and 100 microliters (µL) of 0.1 molar (M) dimethyglyoxime (DMG; Sigma Aldrich) ligand and 130 µL of 0.5 M N-(2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (EPPS, Sigma Aldrich) buffer was added to each sample vial. A Metrohm 858 Sample Processor then loaded 8.5 mL of each sample into the electrode's Teflon cup and added 1.5 mL of 1.5 M NaNO₂ reagent (Merck). The mercury electrode performed a fast linear sweep from -1.4 volts (V) to -0.6 V at a rate of 5 volts per second (V s⁻¹) and produced a cobalt reduction peak at -1.15 V, the voltage at which the Co(DMG)₂ complex is reduced from Co(II) to Co(0) (Saito and Moffett 2001). The height of the Co reduction peak is linearly proportional to the amount of total dCo present in the sample. Peak heights were determined by NOVA 1.10 software. A standard curve was created with 4 additions of 25 picomoles(pM) dCo to each sample, and a type-I linear regression of the addition standard curve performed by the LINEST function in Microsoft Excel allowed for the calculation of the initial amount of Co present in the sample.</p>
<p>When analyzing labile dCo concentrations, samples were not UV-irradiated so as to only quantify the free or weakly bound dCo not bound to strong organic ligands. 11 mL of labile samples were instead allowed to equilibrate with the DMG ligand and EPPS reagent overnight (~8 hours) before analysis so as to allow time for the labile dCo present in the sample to bind to the DMG ligand via competitive ligand exchange (K &gt; 10^16.8). Labile dCo samples were then loaded onto the Sample Processor and analyzed electrochemically using identical methods as described above for total dCo samples.</p>
<p><strong>Known Issues/Problems:</strong><br />
Many of the dCo and labile dCo values measured were unusually low and below the analytical detection limit of 4 pM. In cases where no dCo or labile dCo were detected (i.e. when no peak was measurable and/or the dCo value predicted was &lt; 0 pM), values of 0 pM were assigned for the purposes of plotting and select statistical analysis and were flagged as not detected (n.d.) as well as &lt;DL in the dataset; although these concentrations are not detectable with our methodology, we believe the incredibly low concentrations of dCo and labile dCo observed on this expedition were meaningful, and that removing these values from our analysis misrepresents the data and would skew the results to appear higher than was observed.</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
The height of the Co reduction peak is linearly proportional to the amount of total dCo present in the sample. Peak heights were determined by NOVA 1.10 software. A standard curve was created with 4 additions of 25 pM dCo to each sample, and a type-I linear regression of the addition standard curve performed by the LINEST function in Microsoft Excel allowed for the calculation of the initial amount of Co present in the sample.</p>
<p>Analytical blank measurements for each reagent batch (a unique combination of DMG, EPPS, and NaNO2 reagent batches) were measured to determine any Co contamination due to reagent impurities. Blanks were prepared in triplicate with UV-irradiated surface seawater passed through a column with Chelex 100 resin beads (Bio-Rad) to remove metal contaminants, then UV-irradiated again. Chelex beads were prepared as described in Price et al. (2013) to remove organic impurities from leaching into the eluent. For the 5 batches of reagents used on this expedition, the analytical blanks were found to be 2.3 pM, 4.0 pM, 10.1 pM, 15.6 pM, and 8.6 pM dCo, with an average of 8.1 pM Co. The analytical blank detected for the laboratory-run total dCo samples was 1.0 pM. It should be noted that blank values above 10 pM are considered high for this method. Analytical blank values were subtracted from the measured Co values determined with the respective reagent batch. The average standard deviation within each triplicate batch of blanks (1.3 pM) was used to estimate the analytical limit of detection (3 * blank standard deviation) of 4 pM. When detectable dCo concentrations were found below the 4 pM detection limit, their values were preserved in the dataset and flagged as below the detection limit (&lt;DL).</p>
<p><strong>BCO-DMO Processing:</strong><br />
- removed "NaN" as a missing data indicator&nbsp;(represented as blank/no value in the .csv data file);<br />
- replaced "n.d." with "not detected";<br />
- renamed fields to comply with BCO-DMO naming conventions;<br />
- converted date/time field to ISO 8601 format.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Metrohm 858 Sample Processor
Metrohm 858 Sample Processor
PI Supplied Instrument Name: Metrohm 858 Sample Processor PI Supplied Instrument Description:Metrohm 858 Sample Processor - autosampler that introduces 11 mL of the sample, 1.5 mL of a 1.5 M NaNO2 reagent, and 25 pM standard dCo additions into the sample cup for electrochemical analysis by the 663 VA and µAutolabIII. Instrument Name: Laboratory Autosampler Instrument Short Name: Instrument Description: Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument.
Metrohm 663 VA
Metrohm 663 VA
PI Supplied Instrument Name: Metrohm 663 VA PI Supplied Instrument Description:Metrohm 663 VA and µAutolabIII - Voltammetry analyzer equipped with a hanging mercury drop working electrode. The instrument performed a fast linear sweep from -1.4 V to -0.6 V at a rate of 5 V s-1 and produced a cobalt reduction peak at -1.15 V, the voltage at which the Co(DMG)2 complex is reduced from Co(II) to Co(0). The height of the Co reduction peak is linearly proportional to the amount of total dCo present in the sample. Instrument Name: Metrohm 663 VA Stand mercury electrode Instrument Short Name:Metrohm 663 VA Instrument Description: The Metrohm 663 VA stand forms the wet chemical part of a polarographic and voltammetric analytical system. It features a mercury electrode, an Ag/AgCl reference electrode and a glassy carbon counter electrode. The size of the mercury drop and the stirrer speed are set manually on the VA Stand. The VA Stand can be operated in Dropping Mercury Electrode (DME), Hanging Mercury Drop Electrode (HMDE) and Static Mercury Drop Electrode (SMDE) modes. The VA Stand can be controlled by a potentiostat in conjunction with the Metrohm IME663 interface. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0515/
X-Niskin
X-Niskin
PI Supplied Instrument Name: X-Niskin PI Supplied Instrument Description:Dissolved seawater was collected from full-depth station profiles using a trace metal clean sampling rosette deployed on a conducting synthetic line, both supplied by the U.S. Antarctic Program (USAP), and equipped with twelve 8-liter X-Niskin bottles (Ocean Test Equipment), supplied by the Saito Laboratory. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Metrohm 705 UV Digester
Metrohm 705 UV Digester
PI Supplied Instrument Name: Metrohm 705 UV Digester PI Supplied Instrument Description:Metrohm 705 UV Digester - To measure total dCo concentrations, filtered seawater samples were first UV-irradiated in quartz tubes for one hour in a Metrohm 705 UV Digester to destroy natural ligand-bound Co complexes. Instrument Name: UV Digester Instrument Short Name: Instrument Description: Digestion instrument for UV photolysis of water samples
µAutolabIII
µAutolabIII
PI Supplied Instrument Name: µAutolabIII PI Supplied Instrument Description:Metrohm 663 VA and µAutolabIII - Voltammetry analyzer equipped with a hanging mercury drop working electrode. The instrument performed a fast linear sweep from -1.4 V to -0.6 V at a rate of 5 V s-1 and produced a cobalt reduction peak at -1.15 V, the voltage at which the Co(DMG)2 complex is reduced from Co(II) to Co(0). The height of the Co reduction peak is linearly proportional to the amount of total dCo present in the sample. Instrument Name: Voltammetry Analyzers Instrument Short Name:Voltammetry Analyzers Instrument Description: Instruments that obtain information about an analyte by applying a potential and measuring the current produced in the analyte. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB35/
Cruise: NBP1801
NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1801
Giacomo DiTullio
College of Charleston
https://service.rvdata.us/data/cruise/NBP1801/doc/NBP1801DATA.pdf
Report describing NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel