http://lod.bco-dmo.org/id/dataset/894179 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-04-18 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project) 2023-04-18 publication 2023-04-18 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-05-24 publication https://doi.org/10.26008/1912/bco-dmo.894179.1 Mary Ann Moran University of Georgia principalInvestigator Christopher R. Reisch University of Florida principalInvestigator Catalina Mejia University of Florida principalInvestigator Lidimarie Trujillo Rodriguez University of Florida principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Moran, M. A., Reisch, C. R., Mejia, C., Trujillo Rodriguez, L. (2023) High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.894179.1 [access date] Dataset Description: &lt;p&gt;Each column heading provides the treatment condition and replicate. The first 4 letters are substrate abbreviation (provided below) followed by the bacterial identifier. The bacterial identity is either &quot;WT&quot; indicating the wildtype analog (pooled-RBTnSeq library) or four numbers indicating the locus tag of the disrupted gene (e.g. &quot;1234&quot; represents a mutant of &quot;SPO1234&quot;).&amp;nbsp; Finally, the underscore and lowercase letter (&quot;_a&quot;,&amp;nbsp;&amp;nbsp;&quot;_b&quot;,&amp;nbsp;&amp;nbsp;&quot;_c&quot;,&amp;nbsp;&amp;nbsp;&quot;_d&quot;) indicates the biological replicate.&amp;nbsp;&lt;/p&gt; &lt;p&gt;Values are optical density at 600 nm.&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;strong&gt;Methods &amp;amp; Sampling&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Mutant cultures were pre-grown overnight in ½ YTSS medium with 50&amp;nbsp;mg ml&lt;sup&gt;-1&lt;/sup&gt;&amp;nbsp;kanamycin. Screens were performed in L1 minimal medium&amp;nbsp;(Guillard and Hargraves 1993)&amp;nbsp;(dx.doi.org/10.17504/protocols.io.jvccn2w) modified to a salinity of 20 ppt and amended with ammonium (3 mM), kanamycin (50&amp;nbsp;mg ml&lt;sup&gt;-1&lt;/sup&gt;), and phosphorus as PO&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;3-&lt;/sup&gt;&amp;nbsp;at 36&amp;nbsp;mM. Overnight cultures of individual mutants were washed 3 times in substrate free medium. Four&amp;nbsp;replicate 200 µl mutant cultures were prepared by inoculating 2&amp;nbsp;ml of washed&amp;nbsp; overnight culture into 96 well plates containing 198&amp;nbsp;ml modified L1 medium and a single substrate at 8 mM carbon. As a positive control, four wells with the same medium were inoculated with washed overnight cultures of the pooled-RB-TnSeq library, used as a proxy for wild-type&lt;em&gt;&amp;nbsp;R. pomeroyi&amp;nbsp;&lt;/em&gt;growth but harboring a transposon/kanamycin resistance gene insertion. Cultures were grown at 25&lt;sup&gt;o&lt;/sup&gt;C in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD&lt;sub&gt;600&lt;/sub&gt;&amp;nbsp;readings were collected once each hour.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2019589 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2019589 Funding provided by Simons Foundation (Simons) Award Number: 542391 completed Mary Ann Moran University of Georgia 706-542-6481 Department of Marine Sciences Athens GA 30602 USA mmoran@uga.edu pointOfContact Christopher R. Reisch University of Florida Dept. of Microbiology and Cell Science University of Florida Gainesville FL 32611 USA creisch@ufl.edu pointOfContact Catalina Mejia University of Florida Dept. of Microbiology and Cell Science University of Florida Gainesville FL 32611 USA catalinamejia@ufl.edu pointOfContact Lidimarie Trujillo Rodriguez University of Florida Dept. of Microbiology and Cell Science University of Florida Gainesville FL 32611 USA ltrujillo@ufl.edu pointOfContact asNeeded Dataset Version: 1 Unknown time_hours thymWT_a thymWT_b thymWT_c thymWT_d thym0378_a thym0378_b thym0378_c thym0378_d thym0379_a thym0379_b thym0379_c thym0379_d cholWT_a cholWT_b cholWT_c cholWT_d chol1087_a chol1087_b chol1087_c chol1087_d DMSPWT_a DMSPWT_b DMSPWT_c DMSPWT_d citrWT_a citrWT_b citrWT_c citrWT_d DHPSWT_a DHPSWT_b DHPSWT_c DHPSWT_d glosWT_a glosWT_b glosWT_c glosWT_d xyloWT_a xyloWT_b xyloWT_c xyloWT_d taurWT_a taurWT_b taurWT_c taurWT_d DMSP3186_a DMSP3186_b DMSP3186_c DMSP3186_d xylo0863_a xylo0863_b xylo0863_c xylo0863_d DHPS0591_a DHPS0591_b DHPS0591_c DHPS0591_d glos0863_a glos0863_b glos0863_c glos0863_d taur0676_a taur0676_b taur0676_c taur0676_d taur0674_a taur0674_b taur0674_c taur0674_d glcNWT_a glcNWT_b glcNWT_c glcNWT_d glcN1839_a glcN1839_b glcN1839_c glcN1839_d sperWT_a sperWT_b sperWT_c sperWT_d cadaWT_a cadaWT_b cadaWT_c cadaWT_d putrWT_a putrWT_b putrWT_c putrWT_d ectoWT_a ectoWT_b ectoWT_c ectoWT_d sper3469_a sper3469_b sper3469_c sper3469_d cada3469_a cada3469_b cada3469_c cada3469_d putr3469_a putr3469_b putr3469_c putr3469_d ecto1147_a ecto1147_b ecto1147_c ecto1147_d malaWT_a malaWT_b malaWT_c malaWT_d succWT_a succWT_b succWT_c succWT_d fumrWT_a fumrWT_b fumrWT_c fumrWT_d mala2626_a mala2626_b mala2626_c mala2626_d mala2628_a mala2628_b mala2628_c mala2628_d mala2630_a mala2630_b mala2630_c mala2630_d succ2626_a succ2626_b succ2626_c succ2626_d succ2628_a succ2628_b succ2628_c succ2628_d succ2630_a succ2630_b succ2630_c succ2630_d fumr2626_a fumr2626_b fumr2626_c fumr2626_d fumr2628_a fumr2628_b fumr2628_c fumr2628_d fumr2630_a fumr2630_b fumr2630_c fumr2630_d cystWT_a cystWT_b cystWT_c cystWT_d glycWT_a glycWT_b glycWT_c glycWT_d cyst3040_a cyst3040_b cyst3040_c cyst3040_d glyc0608_a glyc0608_b glyc0608_c glyc0608_d cyst3041_a cyst3041_b cyst3041_c cyst3041_d isetWT_a isetWT_b isetWT_c isetWT_d iset2357_a iset2357_b iset2357_c iset2357_d iset2358_a iset2358_b iset2358_c iset2358_d butrWT_a butrWT_b butrWT_c butrWT_d carnWT_a carnWT_b carnWT_c carnWT_d butr537A_a butr537A_b butr537A_c butr537A_d butr537B_a butr537B_b butr537B_c butr537B_d carn2995_a carn2995_b carn2995_c carn2995_d carn2996_a carn2996_b carn2996_c carn2996_d Synergy H1 plate reader (BioTek, Winooski, VT, USA) theme None, User defined sample time replicate featureType BCO-DMO Standard Parameters plate reader instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Center for Chemical Currencies of a Microbial Planet https://ccomp-stc.org/ Center for Chemical Currencies of a Microbial Planet Functions carried out by microscopic inhabitants of the surface ocean affect every aspect of life on our planet, regardless of distance from the coast. Ocean phytoplankton are responsible for half of the photosynthesis on Earth, the first step in a complex system that annually withdraws 50 billion metric tons of carbon from the atmosphere to sustain their growth. Of this, 25 billion metric tons participate in a rapid cycle in which biologically reactive material is released into seawater and converted back into carbon dioxide by marine bacteria within hours to days. The chemical-microbe network at the heart of this fast cycle remains poorly constrained; consequently, its primary currencies and controls remain elusive; its sensitivities to changing ocean conditions are unknown; and its responses to future climate scenarios are not predictable. The Center for Chemical Currencies of a Microbial Planet (C-CoMP) integrates research, education and knowledge transfer activities to develop a mechanistic understanding of surface ocean carbon flux within the context of a changing ocean and through increased participation in ocean sciences. C-CoMP supports science teams that merge biology, chemistry, modeling, and informatics to close long-standing knowledge gaps in the identities and dynamics of organic molecules that serve as the currencies of elemental transfer between the ocean and atmosphere. C-CoMP fosters education, outreach, and knowledge transfer activities that engage students of all ages, broaden participation in the next generation of ocean scientists, and extend novel open-science approaches into complementary academic and industrial communities. The Center framework is critical to this mission, uniquely facilitating an open exchange of experimental and computational science, methodological and conceptual challenges, and collaborations that establish integrated science and education partnerships. With expanded participation in ocean science research and ocean literacy across the US society, the next generation of ocean scientists will better reflect the diverse US population. Climate-carbon feedbacks on the marine carbon reservoir are major uncertainties for future climate projections, and the trajectory and rate of ocean changes depend directly on microbial responses to temperature increases, ocean acidification, and other perturbations driven by climate change. C-CoMP research closes an urgent knowledge gap in the mechanisms driving carbon flow between ocean and atmosphere, with global implications for predictive climate models. The Center supports interdisciplinary science teams following open and reproducible science practices to address: (1) the chemical currencies of surface ocean carbon flux; (2) the structure and regulation of the chemical-microbe network that mediates this flux; and (3) sensitivity of the network and its feedbacks on climate. C-CoMP leverages emerging tools and technologies to tackle critical challenges in these themes, in synergy with existing ocean programs and consistent with NSF’s Big Ideas. C-CoMP education and outreach activities seek to overcome barriers to ocean literacy and diversify participation in ocean research. The Center is developing (1) initiatives to expand ocean literacy in K-12 and the broader public, (2) ocean sciences undergraduate curricula and research opportunities that provide multiple entry points into research experiences, (3) post-baccalaureate programs to transition undergraduates into graduate education and careers in ocean science, and (4) interdisciplinary graduate student and postdoctoral programs that prepare the next generation of ocean scientists. The C-CoMP team includes education faculty who evaluate the impacts of education and outreach activities and export successful STEM initiatives to the education community. C-CoMP is revolutionizing the technologies for studying chemical transformations in microbial systems to build understanding of the outsized impact of microbes on elemental cycles. Open science, cross-disciplinary collaborations, community engagement, and inclusive practices foster strategic advances in critical science problems and STEM initiatives. C-CoMP science, education, and knowledge-transfer themes are efficiently addressed through a sustained network of scientists addressing critical research challenges while broadening the workforce that will tackle multi-disciplinary problems with academic, industrial and policy partners. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria. The Program's Data Management Plan (DMP) is available as a PDF document. C-CoMP largerWorkCitation program Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites https://osprey.bco-dmo.org/project/884792 Function and Importance of Marine Bacterial Transporters of Plankton Exometabolites <p><strong>Project:</strong></p> <p>Observing expression of marine bacterial transporter systems with transcriptomic or proteomic tools can provide valuable information about the metabolomic environment. However, these ‘omics approaches are limited by the low rate of transporter gene annotation. Here, a barcoded, arrayed, mutant library of the marine bacterium <em>Ruegeria pomeroyi</em> DSS-3 is employed in high throughput screens to identify the target substrates of 13 transporter systems. A set of 156 isolated putative transporter mutants were screened for growth on minimal medium with 63 substrates, each as a sole carbon source. Mutants that demonstrated a growth defect on a specific substrate were selected for secondary, higher resolution, growth screening. Mutants that continued to demonstrate growth defect relative to the pooled-mutant library (pooled-BarSeq, used as an analog for wildtype) were screened for their ability to drawdown the target substate. Gene annotations were made when mutants of the given transporter demonstrated both growth and drawdown defects on the target substrate.</p> <p>In addition to the isolated mutant screens, the pooled barcoded transposon mutant library (pooled-BarSeq) was grown on minimal medium with selected substrates, each as sole carbon source, such that the relative enrichment or depletion of each mutant could demonstrate its fitness cost associated with the loss of each disrupted gene when grown on each substrate. The results of pooled-BarSeq screens had mixed consistency with the isolated mutant screens, demonstrating the value of isolated mutants for transporter annotation.</p> <p><strong>Program:</strong></p> <p>The Center for Chemical Currencies of a Microbial Planet (C-CoMP) integrates research, education and knowledge transfer activities to develop a mechanistic understanding of surface ocean carbon flux within the context of a changing ocean and through increased participation in ocean sciences. C-CoMP supports science teams that merge biology, chemistry, modeling, and informatics to close long-standing knowledge gaps in the identities and dynamics of organic molecules that serve as the currencies of elemental transfer between the ocean and atmosphere.</p> C-CoMP Marine Bacterial Transporters largerWorkCitation project eng; USA oceans 2021-09-01 2022-06-30 0 BCO-DMO catalogue of parameters from High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/895245.rdf Name: time_hours Units: hours Description: <p>Sample time</p> http://lod.bco-dmo.org/id/dataset-parameter/895246.rdf Name: thymWT_a Units: units Description: <p>Thymidine wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895247.rdf Name: thymWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895248.rdf Name: thymWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895249.rdf Name: thymWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895250.rdf Name: thym0378_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0378 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895251.rdf Name: thym0378_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0378 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895252.rdf Name: thym0378_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0378 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895253.rdf Name: thym0378_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0378 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895254.rdf Name: thym0379_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0379 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895255.rdf Name: thym0379_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0379 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895256.rdf Name: thym0379_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0379 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895257.rdf Name: thym0379_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Thymidine locas tag 0379 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895258.rdf Name: cholWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895259.rdf Name: cholWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895260.rdf Name: cholWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895261.rdf Name: cholWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895262.rdf Name: chol1087_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline locas tag 1087 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895263.rdf Name: chol1087_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline locas tag 1087 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895264.rdf Name: chol1087_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline locas tag 1087 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895265.rdf Name: chol1087_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Choline locas tag 1087 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895266.rdf Name: DMSPWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895267.rdf Name: DMSPWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895268.rdf Name: DMSPWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895269.rdf Name: DMSPWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895270.rdf Name: citrWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Citrate wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895271.rdf Name: citrWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Citrate wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895272.rdf Name: citrWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Citrate wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895273.rdf Name: citrWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Citrate wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895274.rdf Name: DHPSWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895275.rdf Name: DHPSWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895276.rdf Name: DHPSWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895277.rdf Name: DHPSWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895278.rdf Name: glosWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895279.rdf Name: glosWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895280.rdf Name: glosWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895281.rdf Name: glosWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895282.rdf Name: xyloWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895283.rdf Name: xyloWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895284.rdf Name: xyloWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895285.rdf Name: xyloWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895286.rdf Name: taurWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine wild type biological replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895287.rdf Name: taurWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine wild type biological replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895288.rdf Name: taurWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine wild type biological replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895289.rdf Name: taurWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine wild type biological replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895290.rdf Name: DMSP3186_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP locas tag 3186 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895291.rdf Name: DMSP3186_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP locas tag 3186 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895292.rdf Name: DMSP3186_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP locas tag 3186 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895293.rdf Name: DMSP3186_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DMSP locas tag 3186 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895294.rdf Name: xylo0863_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose 0863 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895295.rdf Name: xylo0863_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose 0863 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895296.rdf Name: xylo0863_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose 0863 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895297.rdf Name: xylo0863_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Xylose 0863 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895298.rdf Name: DHPS0591_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS locas tag 0591 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895299.rdf Name: DHPS0591_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS locas tag 0591 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895300.rdf Name: DHPS0591_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS locas tag 0591 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895301.rdf Name: DHPS0591_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>DHPS locas tag 0591 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895302.rdf Name: glos0863_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose locas tag 0863 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895303.rdf Name: glos0863_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose locas tag 0863 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895304.rdf Name: glos0863_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose locas tag 0863 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895305.rdf Name: glos0863_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glucose locas tag 0863 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895306.rdf Name: taur0676_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0676 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895307.rdf Name: taur0676_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0676 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895308.rdf Name: taur0676_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0676 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895309.rdf Name: taur0676_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0676 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895310.rdf Name: taur0674_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0674 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895311.rdf Name: taur0674_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0674 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895312.rdf Name: taur0674_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0674 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895313.rdf Name: taur0674_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Taurine locas tag 0674 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895314.rdf Name: glcNWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GlcNAc biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895315.rdf Name: glcNWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895316.rdf Name: glcNWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895317.rdf Name: glcNWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895318.rdf Name: glcN1839_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc locas tag 1839 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895319.rdf Name: glcN1839_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc locas tag 1839 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895320.rdf Name: glcN1839_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc locas tag 1839 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895321.rdf Name: glcN1839_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>GLcNAc locas tag 1839 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895322.rdf Name: sperWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895323.rdf Name: sperWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895324.rdf Name: sperWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895325.rdf Name: sperWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895326.rdf Name: cadaWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895327.rdf Name: cadaWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895328.rdf Name: cadaWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895329.rdf Name: cadaWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895330.rdf Name: putrWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895331.rdf Name: putrWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895332.rdf Name: putrWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895333.rdf Name: putrWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895334.rdf Name: ectoWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895335.rdf Name: ectoWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895336.rdf Name: ectoWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895337.rdf Name: ectoWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895338.rdf Name: sper3469_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine locas tag 3469 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895339.rdf Name: sper3469_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine locas tag 3469 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895340.rdf Name: sper3469_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine locas tag 3469 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895341.rdf Name: sper3469_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Spermidine locas tag 3469 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895342.rdf Name: cada3469_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine locas tag 3469 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895343.rdf Name: cada3469_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine locas tag 3469 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895344.rdf Name: cada3469_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine locas tag 3469 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895345.rdf Name: cada3469_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cadaverine locas tag 3469 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895346.rdf Name: putr3469_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine locas tag 3469 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895347.rdf Name: putr3469_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine locas tag 3469 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895348.rdf Name: putr3469_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine locas tag 3469 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895349.rdf Name: putr3469_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Putresceine locas tag 3469 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895350.rdf Name: ecto1147_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine locas tag 1147 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895351.rdf Name: ecto1147_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine locas tag 1147 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895352.rdf Name: ecto1147_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine locas tag 1147 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895353.rdf Name: ecto1147_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Ectoine locas tag 1147 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895354.rdf Name: malaWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895355.rdf Name: malaWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895356.rdf Name: malaWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895357.rdf Name: malaWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895358.rdf Name: succWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895359.rdf Name: succWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895360.rdf Name: succWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895361.rdf Name: succWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895362.rdf Name: fumrWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895363.rdf Name: fumrWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895364.rdf Name: fumrWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895365.rdf Name: fumrWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895366.rdf Name: mala2626_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2626 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895367.rdf Name: mala2626_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2626 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895368.rdf Name: mala2626_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2626 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895369.rdf Name: mala2626_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2626 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895370.rdf Name: mala2628_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2628 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895371.rdf Name: mala2628_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2628 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895372.rdf Name: mala2628_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2628 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895373.rdf Name: mala2628_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2628 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895374.rdf Name: mala2630_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2630 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895375.rdf Name: mala2630_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2630 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895376.rdf Name: mala2630_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2630 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895377.rdf Name: mala2630_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Malate locus tag 2630 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895378.rdf Name: succ2626_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2626 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895379.rdf Name: succ2626_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2626 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895380.rdf Name: succ2626_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2626 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895381.rdf Name: succ2626_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2626 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895382.rdf Name: succ2628_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2628 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895383.rdf Name: succ2628_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2628 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895384.rdf Name: succ2628_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2628 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895385.rdf Name: succ2628_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2628 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895386.rdf Name: succ2630_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2630 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895387.rdf Name: succ2630_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2630 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895388.rdf Name: succ2630_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2630 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895389.rdf Name: succ2630_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Succinate locus tag 2630 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895390.rdf Name: fumr2626_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2626 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895391.rdf Name: fumr2626_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2626 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895392.rdf Name: fumr2626_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2626 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895393.rdf Name: fumr2626_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2626 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895394.rdf Name: fumr2628_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2628 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895395.rdf Name: fumr2628_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2628 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895396.rdf Name: fumr2628_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2628 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895397.rdf Name: fumr2628_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2628 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895398.rdf Name: fumr2630_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2630 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895399.rdf Name: fumr2630_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2630 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895400.rdf Name: fumr2630_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2630 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895401.rdf Name: fumr2630_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Fumarate locus tag 2630 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895402.rdf Name: cystWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895403.rdf Name: cystWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895404.rdf Name: cystWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895405.rdf Name: cystWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895406.rdf Name: glycWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895407.rdf Name: glycWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895408.rdf Name: glycWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895409.rdf Name: glycWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895410.rdf Name: cyst3040_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3040 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895411.rdf Name: cyst3040_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3040 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895412.rdf Name: cyst3040_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3040 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895413.rdf Name: cyst3040_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3040 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895414.rdf Name: glyc0608_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol locus tag 0608 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895415.rdf Name: glyc0608_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol locus tag 0608 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895416.rdf Name: glyc0608_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol locus tag 0608 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895417.rdf Name: glyc0608_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Glycerol locus tag 0608 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895418.rdf Name: cyst3041_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3041 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895419.rdf Name: cyst3041_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3041 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895420.rdf Name: cyst3041_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3041 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895421.rdf Name: cyst3041_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Cysteate locus tag 3041 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895422.rdf Name: isetWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895423.rdf Name: isetWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895424.rdf Name: isetWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895425.rdf Name: isetWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895426.rdf Name: iset2357_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2357 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895427.rdf Name: iset2357_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2357 repilicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895428.rdf Name: iset2357_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2357 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895429.rdf Name: iset2357_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2357 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895430.rdf Name: iset2358_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2358 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895431.rdf Name: iset2358_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2358 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895432.rdf Name: iset2358_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2358 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895433.rdf Name: iset2358_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Isethionate locus tag 2358 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895434.rdf Name: butrWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895435.rdf Name: butrWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895436.rdf Name: butrWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895437.rdf Name: butrWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895438.rdf Name: carnWT_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine biological wild type replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895439.rdf Name: carnWT_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine biological wild type replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895440.rdf Name: carnWT_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine biological wild type replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895441.rdf Name: carnWT_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine biological wild type replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895442.rdf Name: butr537A_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537A replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895443.rdf Name: butr537A_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537A replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895444.rdf Name: butr537A_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537A replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895445.rdf Name: butr537A_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537A replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895446.rdf Name: butr537B_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537B replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895447.rdf Name: butr537B_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537B replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895448.rdf Name: butr537B_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537B replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895449.rdf Name: butr537B_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>3-Hydroxybutyrate locus tag 537B replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895450.rdf Name: carn2995_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2995 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895451.rdf Name: carn2995_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2995 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895452.rdf Name: carn2995_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2995 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895453.rdf Name: carn2995_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2995 replicate d</p> http://lod.bco-dmo.org/id/dataset-parameter/895454.rdf Name: carn2996_a Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2996 replicate a</p> http://lod.bco-dmo.org/id/dataset-parameter/895455.rdf Name: carn2996_b Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2996 replicate b</p> http://lod.bco-dmo.org/id/dataset-parameter/895456.rdf Name: carn2996_c Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2996 replicate c</p> http://lod.bco-dmo.org/id/dataset-parameter/895457.rdf Name: carn2996_d Units: Number of sequence counts (barcodes) that mapped to each gene in the treatment. Description: <p>Carnitine locus tag 2996 replicate d</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 36185 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/0d6e51ed-9b09-40ce-8461-c0cd16b518cc/content download https://doi.org/10.26008/1912/bco-dmo.894179.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Methods &amp;amp; Sampling&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;Mutant cultures were pre-grown overnight in ½ YTSS medium with 50&amp;nbsp;mg ml&lt;sup&gt;-1&lt;/sup&gt;&amp;nbsp;kanamycin. Screens were performed in L1 minimal medium&amp;nbsp;(Guillard and Hargraves 1993)&amp;nbsp;(dx.doi.org/10.17504/protocols.io.jvccn2w) modified to a salinity of 20 ppt and amended with ammonium (3 mM), kanamycin (50&amp;nbsp;mg ml&lt;sup&gt;-1&lt;/sup&gt;), and phosphorus as PO&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;3-&lt;/sup&gt;&amp;nbsp;at 36&amp;nbsp;mM. Overnight cultures of individual mutants were washed 3 times in substrate free medium. Four&amp;nbsp;replicate 200 µl mutant cultures were prepared by inoculating 2&amp;nbsp;ml of washed&amp;nbsp; overnight culture into 96 well plates containing 198&amp;nbsp;ml modified L1 medium and a single substrate at 8 mM carbon. As a positive control, four wells with the same medium were inoculated with washed overnight cultures of the pooled-RB-TnSeq library, used as a proxy for wild-type&lt;em&gt;&amp;nbsp;R. pomeroyi&amp;nbsp;&lt;/em&gt;growth but harboring a transposon/kanamycin resistance gene insertion. Cultures were grown at 25&lt;sup&gt;o&lt;/sup&gt;C in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD&lt;sub&gt;600&lt;/sub&gt;&amp;nbsp;readings were collected once each hour.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;div class=&quot;field&quot; id=&quot;form_data_processing&quot;&gt; &lt;p&gt;&lt;strong&gt;Data Processing Notes from Submitter:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;The data were imported into Excel and compiled on a single sheet. Values are OD600, they have not been pathlength corrected, they have not been blank corrected. We recommend blanking each sample to its first time point.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;BCO-DMO Processing Notes:&lt;/strong&gt;&lt;/p&gt; &lt;ul&gt; &lt;li&gt;Replaced spaces and special characters in column header names with underscores (&quot;_&quot;)&lt;/li&gt; &lt;/ul&gt; &lt;/div&gt; &lt;div class=&quot;field&quot; id=&quot;form_problems&quot;&gt;&amp;nbsp;&lt;/div&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Synergy H1 plate reader (BioTek, Winooski, VT, USA) Synergy H1 plate reader (BioTek, Winooski, VT, USA) PI Supplied Instrument Name: Synergy H1 plate reader (BioTek, Winooski, VT, USA) PI Supplied Instrument Description:Cultures were grown at 25oC in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD600 readings were collected once each hour. Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.