http://lod.bco-dmo.org/id/dataset/896169
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-05-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrients from CTD casts conducted on R/V Hugh R. Sharp cruise HRS2212 in the Chesapeake Bay during August 2022
2023-05-31
publication
2023-05-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-06-22
publication
https://doi.org/10.26008/1912/bco-dmo.896169.1
Bess B. Ward
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B. (2023) Nutrients from CTD casts conducted on R/V Hugh R. Sharp cruise HRS2212 in the Chesapeake Bay during August 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-05-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.896169.1 [access date]
Chesapeake Bay Nutrients 2022 Dataset Description: Methods and Sampling: <p>Water samples were collected using a 12 x 10-liter&nbsp;Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.), a sensor for dissolved oxygen (SBE43, Sea-Bird), and a sensor for chlorophyll fluorescence (FluoroWetlabECO, AFL FL Sensor). NH4 and NO2 were measured on board immediately after sample collection. NO3 was assayed in the home laboratory on samples stored frozen at the time of collection.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1946516 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1946516
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cast
Station
Date
Latitude
Longitude
Depth
NH4
NH4_stdev
NO2
NO2_stdev
NO3
NO3_stdev
Teledyne Chemiluminescence NO/NOx Analyzer (Model 200E)
10-liter Niskin bottle rosette
Turner Designs Trilogy Fluorometer
UV-visible spectrophotometer (UV-2450, Shimadzu, Japan)
theme
None, User defined
cast
station
date
latitude
longitude
depth
Ammonium
Nitrite
Nitrate
featureType
BCO-DMO Standard Parameters
Chemiluminescence NOx Analyzer
Niskin bottle
Turner Designs Trilogy fluorometer
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
HRS2212
service
Deployment Activity
Chesapeake Bay
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Nitrite Oxidation in Oxygen Minimum Zones
https://osprey.bco-dmo.org/project/831333
Nitrite Oxidation in Oxygen Minimum Zones
<p><em>NSF Award Abstract:</em><br />
This research is grounded in the fundamental role of nitrogen in limiting production in the ocean. Nitrite is a pivotal compound in the nitrogen cycle: it can be oxidized to nitrate, and thus retained as an available nutrient, or it can be reduced to dinitrogen gas, and thus lost from the bioavailable nitrogen pool. Oxidation of nitrite by nitrite oxidizing bacteria (NOB) is the only biological pathway by which nitrate is produced, and all known NOB require oxygen for life. The reduction pathway is also carried out by microbes, in this case, bacteria that thrive only in the absence of oxygen. In previous experiments, however, both oxidation and reduction of nitrite were detected in the same samples from ocean waters in the absence of oxygen. We will investigate three explanations for the apparent oxidation of nitrite in the absence of oxygen on a research cruise to the low oxygen waters off the coast of Peru: 1) The presence of unknown kinds of NOB that do not require oxygen; 2) a new reaction called dismutation, which is possible but never detected in nature; 3) an artifact associated with oxygen stress in NOB. This research could lead to discovery of novel mechanisms and or novel organisms that determine the fate of nitrite and the availability of nitrogen to support primary production in the long run. This project will advance discovery and understanding while promoting teaching, training and learning by providing opportunities for Princeton students to get involved in and have hands on experience in research in the lab and potentially at sea. Both undergraduate and graduate students will participate in the research through internships and field experiences. We will also integrate our work at sea into teaching in the classroom via videos and assignments based on data collected during the cruise.</p>
<p>Nitrite oxidation is the only known biological process that produces nitrate, which comprises the largest fixed nitrogen reservoir in the ocean. Nitrite oxidation is carried out by nitrite oxidizing bacteria (NOB), and all known species are obligate aerobes. Nitrite reduction to N2 occurs in multiple microbial pathways, generally under anoxic conditions. Despite their apparent incompatibility regarding oxygen, both processes are detected in the low oxygen or anoxic waters of oxygen minimum zones (OMZs). Thus, the fate of nitrite in OMZs has implications for the global fixed N budget. Nitrite oxidation is detected at high rates in essentially zero oxygen water in the most oxygen depleted depth intervals in OMZ regions, which suggests that some nitrite oxidizers might possess anaerobic metabolic capabilities. Nitrite disproportionation (or dismutation), in which nitrite is simultaneously oxidized to nitrate and reduced to N2, is a thermodynamically favorable reaction, which would link the two processes in one organism – but it has never been observed in nature. The research proposed here will address two big questions about nitrite in the ocean: 1) How does anaerobic nitrite oxidation work? 2) What determines the fate of nitrite? The experimental approach will investigate three possible explanations for anaerobic nitrite oxidation: 1) Nitrite is oxidized to nitrate by different clades of NOB, which exhibit different tolerances/requirements for oxygen; 2) Nitrite dismutation, also performed by NOB, partially explains the cooccurrence of oxidation and reduction of nitrite; 3) Apparently anaerobic nitrite oxidation is indeed biologically mediated but does not always represent net production of nitrate from nitrite; rather it results from isotopic equilibration during enzyme-catalyzed interconversion of nitrite and nitrate. These questions will be addressed by performing a suite of 15N-tracer incubations at stations located within and outside of one of the major OMZs in the ocean, the Eastern Tropical South Pacific. The dependence of the rate processes on oxygen concentrations will be determined, and the composition of the microbial assemblages will be assessed in order to determine whether different microbial components are involved under different environmental conditions. The expression of genes involved in oxidation/reduction/ respiratory metabolisms at low oxygen concentrations will be measured across oxygen gradients and in oxygen manipulations to identify their potential role in supporting “anaerobic” nitrite oxidation. The possibility that the apparently anaerobic nitrite oxidation is due to an enzyme level interconversion between nitrite and nitrate, which does not lead to net nitrate production and is not linked to growth of nitrite oxidizing bacteria, will also be investigated.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
NO2Ox_OMZs
largerWorkCitation
project
eng; USA
oceans
Chesapeake Bay
-76.4407
-76.3665
38.5652
38.9972
2022-08-12
2022-08-19
Eastern Tropical South Pacific and Chesapeake Bay
0
BCO-DMO catalogue of parameters from Nutrients from CTD casts conducted on R/V Hugh R. Sharp cruise HRS2212 in the Chesapeake Bay during August 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/896687.rdf
Name: Cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/896688.rdf
Name: Station
Units: unitless
Description: <p>station name</p>
http://lod.bco-dmo.org/id/dataset-parameter/896689.rdf
Name: Date
Units: unitless
Description: <p>date of cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/896690.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude (positive values = North)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896691.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude (negative values = West)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896692.rdf
Name: Depth
Units: meters (m)
Description: <p>depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/896693.rdf
Name: NH4
Units: micromolar (uM)
Description: <p>ammonium concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/896694.rdf
Name: NH4_stdev
Units: micromolar (uM)
Description: <p>ammonium concentration standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/896695.rdf
Name: NO2
Units: micromolar (uM)
Description: <p>nitrite concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/896696.rdf
Name: NO2_stdev
Units: micromolar (uM)
Description: <p>nitrite concentration standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/896697.rdf
Name: NO3
Units: micromolar (uM)
Description: <p>nitrate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/896698.rdf
Name: NO3_stdev
Units: micromolar (uM)
Description: <p>nitrate concentration standard deviation</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6464
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c0a8cf60-da11-4e56-a5b5-956312384da7/content
download
https://doi.org/10.26008/1912/bco-dmo.896169.1
download
onLine
dataset
<p>Water samples were collected using a 12 x 10-liter&nbsp;Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.), a sensor for dissolved oxygen (SBE43, Sea-Bird), and a sensor for chlorophyll fluorescence (FluoroWetlabECO, AFL FL Sensor). NH4 and NO2 were measured on board immediately after sample collection. NO3 was assayed in the home laboratory on samples stored frozen at the time of collection.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- renamed fields to comply with BCO-DMO naming conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Teledyne Chemiluminescence NO/NOx Analyzer (Model 200E)
Teledyne Chemiluminescence NO/NOx Analyzer (Model 200E)
PI Supplied Instrument Name: Teledyne Chemiluminescence NO/NOx Analyzer (Model 200E) PI Supplied Instrument Description:Used to measure NO3 Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX.
One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200
10-liter Niskin bottle rosette
10-liter Niskin bottle rosette
PI Supplied Instrument Name: 10-liter Niskin bottle rosette Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Turner Designs Trilogy Fluorometer
Turner Designs Trilogy Fluorometer
PI Supplied Instrument Name: Turner Designs Trilogy Fluorometer PI Supplied Instrument Description:Used to measure NH4 Instrument Name: Turner Designs Trilogy fluorometer Instrument Short Name:Turner Trilogy Instrument Description: The Trilogy Laboratory Fluorometer is a compact laboratory instrument for making fluorescence, absorbance, and turbidity measurements using the appropriate snap-in application module. Fluorescence modules are available for discrete sample measurements of various fluorescent materials including chlorophyll (in vivo and extracted), rhodamine, fluorescein, cyanobacteria pigments, ammonium, CDOM, optical brighteners, and other fluorescent compounds.
UV-visible spectrophotometer (UV-2450, Shimadzu, Japan)
UV-visible spectrophotometer (UV-2450, Shimadzu, Japan)
PI Supplied Instrument Name: UV-visible spectrophotometer (UV-2450, Shimadzu, Japan) PI Supplied Instrument Description:Used to measure NO2 and urea Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: HRS2212
HRS2212
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel
HRS2212
Amal Jayakumar
Princeton University
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel