http://lod.bco-dmo.org/id/dataset/896181
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-05-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Field physiochemical parameters including nutrient concentrations and nitrogen specific uptake rates from samples collected between 2017 and 2019 from the Arctic Ocean, California Coastal Current, and a Chesapeake Bay estuary
2023-05-17
publication
2023-05-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-06-05
publication
https://doi.org/10.26008/1912/bco-dmo.896181.1
Deborah A. Bronk
Virginia Institute of Marine Science
principalInvestigator
Boris Wawrik
University of Oklahoma
principalInvestigator
Zhibo Yang
University of Oklahoma
principalInvestigator
Brianna Stanley
Virginia Institute of Marine Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Bronk, D. A., Wawrik, B., Yang, Z., Stanley, B. (2023) Field physiochemical parameters including nutrient concentrations and nitrogen specific uptake rates from samples collected between 2017 and 2019 from the Arctic Ocean, California Coastal Current, and a Chesapeake Bay estuary. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-05-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.896181.1 [access date]
Field Physiochemical Parameters and Nitrogen Specific Uptake Rates Dataset Description: Methods and Sampling: <p>Site water for incubation experiments and for the analysis of ambient nutrients was collected slightly differently for each of the sampling regions. In the Arctic, site water for incubation experiments was collected on the R/V Ukpik and R/V Sikuliaq (cruise ID number SK201712S) during the summer of 2017 using a submersible pump and CTD rosette, respectively. In Baja, sampling occurred twice on the R/V Robert Gordon Sproul during both May and October 2017 (cruise ID numbers SP1714 and SP1727), and site water was collected using a CTD rosette (Turk-Kubo et al. 2021). In Virginia, surface sites in the York River were sampled by directly filling an acid-washed PETG carboy over the side. Filtrations for sample filtrate and filters also varied. These York Rivers samples were obtained during day trips aboard a variety of small powerboats (&lt;21 feet), every other month from June 2018 to July 2019. Powerboats are housed out of the Virginia Institute of Marine Science.</p>
<p>In the Arctic, filtrations were conducted in parallel, which separately passed site water through a larger Nucleopore Membrane filter (3.0 micrometer (μm) nominal pore size) and a smaller Whatman GF-75 filter (nominal pore size 0.3 μm). This same combination of filters was used in Baja in October 2017. In May 2017, Baja sampling used the same filters, but filtered sequentially, meaning that site water was first passed through the Nucleopore filter before passing through the GF-75. The York River site samples were only filtered using the GF-75. Filtrate for all regions was collected from the GF-75 filtration and kept for later nutrient analyses and filters were used for chlorophyll <em>a </em>analysis.</p>
<p>Pigments (chlorophyll <em>a</em> and phaeopigments) were measured after extraction with 90% acetone overnight (Parsons et al. 1984; Arar and Collins 1997). Concentrations of ammonium were analyzed using the Koroleff (1983) method and amino acids were measured as dissolved primary amines (DPA; Parsons et al. 1984). Concentrations of nitrate, nitrite, phosphate, and silica were measured using a Lachat 8500 Quickchem autoanalyzer. Urea was analyzed using the monoxime method (Price and Harrison 1987). Concentrations of total dissolved nitrogen and dissolved organic carbon were assessed using a Shimadzu TOC-V TNM (Hansell 1993). Dissolved organic nitrogen is calculated as the difference between total dissolved nitrogen and inorganic nitrogen.&nbsp;</p>
<p>Nitrogen uptakes were measured after incubation for a set time (1 to 24 hours) in either 0.5 or 1-liter PETG bottles. Stable isotope tracer methods were used according to those described in Baer et al (2017). Uptake rates for inorganic and organic nitrogen were measured by incubating water with 15N- ammonium, nitrate, creatine, urea, and/or an amino acid mixture under <em>in situ</em> light and temperature conditions in a flow-through system on deck or in a cold room set to ambient site water temperatures. Rate incubation experiments were terminated with the same filtration methods as collection of site water ambients with the exception that a Sterlitech silver filter was used in lieu of the Nucleopore Membrane filter. The exact combination of nitrogen substrates varied between sites and regions. All nitrogen uptake samples were analyzed on a Sercon Integra 2 isotope ratio mass spectrometer.</p>
<p><strong>Known Issues/Problems:</strong><br />
Note that for the October sampling in the Baja region, chlorophyll <em>a</em> concentrations for the nutrient size fractions are unavailable. Instead, chlorophyll <em>a</em> concentrations for the &gt;0.7 μm size fraction are available thanks to the Arrigo and Zehr dataset (listed under "Related Datasets").</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635369 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635369
completed
Deborah A. Bronk
Virginia Institute of Marine Science
207-315-2567
60 Bigelow Drive
East Boothbay
ME
04544
USA
dbronk@bigelow.org
pointOfContact
Boris Wawrik
University of Oklahoma
301-903-4742
Burke
VA
USA
Boris.Wawrik@science.doe.gov
pointOfContact
Zhibo Yang
University of Oklahoma
405-325-1772
101 Stephenson Parkway Department of Chemistry and Biochemistry
Norman
Oklahoma
73019
USA
zhibo.yang@ou.edu
pointOfContact
Brianna Stanley
Virginia Institute of Marine Science
60 Bigelow Drive
East Boothbay
ME
04544
USA
bstanley@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Ship
Region
Designator
Site_Name
Latitude
Longitude
Date
Time
Time_Zone
ISO_DateTime_UTC
Water_Depth
Sample_Depth
Total_Water_Column_Depth
Salinity
Temperature
Total_Dissolved_N
Total_Dissolved_Nz_Std
Nitrite
Nitrite_Std
Nitrate
Nitrate_Std
Ammonium
Ammonium_Std
Dissolved_Organic_N
Dissolved_Organic_N_Std
Urea
Urea_Std
Dissolved_Primary_Amines
Dissolved_Primary_Amines_Std
Dissolved_Organic_C
Dissolved_Organic_C_Std
Phosphate
Phosphate_Std
Silicate
Silicate_Std
Chl_a_gt_3um
Chl_a_gt_3um_Std
Chl_a_gt_7tenths_um
Chl_a_gt_7tenths_um_Std
Phaeo_gt_3um
Phaeo_gt_3um_Std
Chl_a_gt_3tenths_um
Chl_a_gt_3tenths_um_Std
Phaeo_gt_3tenths_um
Phaeo_gt_3tenths_um_Std
Creatine_V_gt_3um
Creatine_V_gt_3um_Std
Creatine_V_3tenths_to_3um
Creatine_V_3tenths_to_3um_Std
Creatine_V_gt_3tenths_um
Creatine_V_gt_3tenths_um_Std
Isotope_Dilution_Corrected_Ammonium_V_gt_3um
Isotope_Dilution_Corrected_Ammonium_V_gt_3um_Std
Isotope_Dilution_Corrected_Ammonium_V_gt_3tenths_um
Isotope_Dilution_Corrected_Ammonium_V_gt_3tenths_um_Std
Ammonium_V_gt_3um
Ammonium_V_gt_3um_Std
Ammonium_V_3tenths_to_3um
Ammonium_V_3tenths_to_3um_Std
Ammonium_V_gt_3tenths_um
Ammonium_V_gt_3tenths_um_Std
Nitrate_V_gt_3um
Nitrate_V_gt_3um_Std
Nitrate_V_gt_3tenths_um
Nitrate_V_gt_3tenths_um_Std
Urea_V_gt_3um
Urea_V_gt_3um_Std
Urea_V_gt_3tenths_um
Urea_V_gt_3tenths_um_Std
AA_V_gt_3um
AA_V_gt_3um_Std
AA_V_3tenths_to_3um
AA_V_3tenths_to_3um_Std
AA_V_gt_3tenths_um
AA_V_gt_3tenths_um_Std
Sercon Integra2 Mass Spectrometer
8-liter Niskin bottles
Lachat QuickChem 8500 autoanalyzer
Johnson Pump model # 16004
Shimadzu 5000A TOC-V/TNM
Shimadzu RF-600 Spectrofluorophotometer
Turner Design Model 10-AU fluorometer
Shimadzu UV-1800 spectrophotometer
theme
None, User defined
ship
region
Cruise identfier
site
latitude
longitude
date
time of day
time_zone
ISO_DateTime_UTC
sample description
depth
depth_w
salinity
water temperature at measurement depth
Total Dissolved Nitrogen
Nitrite
Nitrate
Ammonium
Dissolved Organic Nitrogen
Urea
primary_amines
dissolved organic Carbon
phosphate
Silicate, Si(OH)4, silicic acid
chlorophyll a
total phaeopigment
creatine
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Niskin bottle
Nutrient Autoanalyzer
Pump
Shimadzu TOC-V Analyzer
Spectrophotometer
Turner Designs Fluorometer 10-AU
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
Ukpik_July-August_2017
SKQ201712S
SP1714
SP1727
service
Deployment Activity
California Current waters off the coast of Southern California and Baja California
Southern California Current waters
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Creatine Cycling in Marine Bacterial and Phytoplankton Assemblages
https://osprey.bco-dmo.org/project/709493
Collaborative Research: Creatine Cycling in Marine Bacterial and Phytoplankton Assemblages
<p><em>NSF Award Abstract:</em><br />
High rates of dissolved organic nitrogen (DON) production and utilization in aquatic systems are typically attributed to microbial activity. Though it is known that there is a tight coupling between the production and consumption of biologically available DON, the composition, dynamics, and ecological significance of this rapidly cycled DON pool are less well understood. This proposal focuses on a component of the DON pool, creatine, which is historically understood as a product of metazoan activity, but appears to be both produced by phytoplankton and consumed by marine bacteria. Creatine is present in seawater in measurable quantities, which led to the hypothesis that creatine may be a significant component of the marine DON cycle. DON cycling likely has a bearing on fundamental marine ecosystem processes with large implications for carbon and nitrogen turnover on a global scale. Broader impacts of this project will include outreach that focuses on connecting scientists with K-12 students through research experiences for teachers and lesson development in collaboration with the K20 Center for Educational and Community Renewal, a statewide education research and development center at the University of Oklahoma. The project will integrate the research with inquiry-based teaching of rural secondary science teachers through Authentic Research Experiences in oceanographic science and microbial ecology. The K20 network includes 96% of Oklahoma schools, providing a unique opportunity to impact STEM education in Oklahoma.</p>
<p>The results of this project will help develop a better understanding of DON cycling, the ecological context of creatine uptake activity, and identify both creatine-producing and consuming organisms in the marine environment. The importance of creatine cycling will be assessed via 15N tracer studies along the natural coastal-to-offshore productivity gradient observed in the North Atlantic. Tracer and molecular approaches will be used to investigate the importance of phytoplankton vs. bacteria in creatine uptake and, the taxonomic identities of creatine-utilizing bacteria will be interrogated via molecular, stable isotope probing (SIP), and RT-qPCR approaches.</p>
Creatine Cycling
largerWorkCitation
project
eng; USA
oceans
California Current waters off the coast of Southern California and Baja California; Southern California Current waters
-115.91
-76.687
28.29
72.55
2017-05-03
2019-07-16
Atlantic bight
0
BCO-DMO catalogue of parameters from Field physiochemical parameters including nutrient concentrations and nitrogen specific uptake rates from samples collected between 2017 and 2019 from the Arctic Ocean, California Coastal Current, and a Chesapeake Bay estuary
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/896310.rdf
Name: Ship
Units: unitless
Description: <p>Name of research vessel</p>
http://lod.bco-dmo.org/id/dataset-parameter/896311.rdf
Name: Region
Units: unitless
Description: <p>General geogrpaphic region for the data collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/896312.rdf
Name: Designator
Units: unitless
Description: <p>Numerical cruise number or other descriptor</p>
http://lod.bco-dmo.org/id/dataset-parameter/896313.rdf
Name: Site_Name
Units: unitless
Description: <p>Name or descriptor of sampled site location</p>
http://lod.bco-dmo.org/id/dataset-parameter/896314.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude (the North direction is represented by positive numbers)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896315.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude (the West direction is represented by negative numbers)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896316.rdf
Name: Date
Units: unitless
Description: <p>Date of sample collection in the local time zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/896317.rdf
Name: Time
Units: unitless
Description: <p>Time of sample collection recorded in the respective local time using a 24 hour clock</p>
http://lod.bco-dmo.org/id/dataset-parameter/896318.rdf
Name: Time_Zone
Units: unitless
Description: <p>Respective local time zone where AKDT is Alaska Daylight Time, EST is Eastern Standard Time, and EDT is Eastern Daylight Time</p>
http://lod.bco-dmo.org/id/dataset-parameter/896319.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample collection in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/896320.rdf
Name: Water_Depth
Units: unitless
Description: <p>General descriptor (e.g., surface, Chlorophyll maximum, etc.)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896321.rdf
Name: Sample_Depth
Units: meters (m)
Description: <p>Depth sample collected at in meters</p>
http://lod.bco-dmo.org/id/dataset-parameter/896322.rdf
Name: Total_Water_Column_Depth
Units: meters (m)
Description: <p>Depth of the total water column in meters</p>
http://lod.bco-dmo.org/id/dataset-parameter/896323.rdf
Name: Salinity
Units: parts per trillion
Description: <p>Salinity of site water in parts per trillion</p>
http://lod.bco-dmo.org/id/dataset-parameter/896324.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Temperature of site water in degrees Celsius</p>
http://lod.bco-dmo.org/id/dataset-parameter/896325.rdf
Name: Total_Dissolved_N
Units: micromoles per liter (umol/L)
Description: <p>Total dissolved nitrogen in micromoles nitrogen per liter</p>
http://lod.bco-dmo.org/id/dataset-parameter/896326.rdf
Name: Total_Dissolved_Nz_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Total_Dissolved_N</p>
http://lod.bco-dmo.org/id/dataset-parameter/896327.rdf
Name: Nitrite
Units: micromoles per liter (umol/L)
Description: <p>Nitrite in micromoles nitrogen per liter; 0.000 = Below detection; Detection limit: 0.05 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896328.rdf
Name: Nitrite_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Nitrite. "SL" means "Sample lost".</p>
http://lod.bco-dmo.org/id/dataset-parameter/896329.rdf
Name: Nitrate
Units: micromoles per liter (umol/L)
Description: <p>Nitrate in micromoles nitrogen per liter; 0.000 = Below detection; Detection limit: 0.05 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896330.rdf
Name: Nitrate_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/896331.rdf
Name: Ammonium
Units: micromoles per liter (umol/L)
Description: <p>Ammonium in micromoles nitrogen per liter; 0.000 = Below detection; Detection limit: 0.03 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896332.rdf
Name: Ammonium_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Ammonium</p>
http://lod.bco-dmo.org/id/dataset-parameter/896333.rdf
Name: Dissolved_Organic_N
Units: micromoles per liter (umol/L)
Description: <p>Dissolved organic nitrogen in micromoles nitrogen per liter</p>
http://lod.bco-dmo.org/id/dataset-parameter/896334.rdf
Name: Dissolved_Organic_N_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Dissolved_Organic_N. "SL" means "Sample lost".</p>
http://lod.bco-dmo.org/id/dataset-parameter/896335.rdf
Name: Urea
Units: micromoles per liter (umol/L)
Description: <p>Urea in micromoles nitrogen per liter; 0.000 = Below detection; Detection limit: 0.1 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896336.rdf
Name: Urea_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Urea</p>
http://lod.bco-dmo.org/id/dataset-parameter/896337.rdf
Name: Dissolved_Primary_Amines
Units: micromoles per liter (umol/L)
Description: <p>Dissolved primary amine in micromoles nitrogen per liter; 0.000 = Below detection; Detection limit: 0.025 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896338.rdf
Name: Dissolved_Primary_Amines_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Dissolved_Primary_Amines</p>
http://lod.bco-dmo.org/id/dataset-parameter/896339.rdf
Name: Dissolved_Organic_C
Units: micromoles per liter (umol/L)
Description: <p>Dissolved organic carbon in micromoles carbon per liter</p>
http://lod.bco-dmo.org/id/dataset-parameter/896340.rdf
Name: Dissolved_Organic_C_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Dissolved_Organic_C</p>
http://lod.bco-dmo.org/id/dataset-parameter/896341.rdf
Name: Phosphate
Units: micromoles per liter (umol/L)
Description: <p>Phosphate in micromoles phosphorus per liter; 0.000 = Below detection; Detection limit: 0.05 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896342.rdf
Name: Phosphate_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/896343.rdf
Name: Silicate
Units: micromoles per liter (umol/L)
Description: <p>Silicate in micromoles silica per liter; 0.000 = Below detection; Detection limit: 0.11 umol N L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896344.rdf
Name: Silicate_Std
Units: micromoles per liter (umol/L)
Description: <p>One standard deviation of Silicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/896345.rdf
Name: Chl_a_gt_3um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyl a in micrograms per liter in the size fraction representing the community greater than 3.0 micrometers (>3.0um); 0.000 = Below detection; Detection limit: 0.025 ug L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896346.rdf
Name: Chl_a_gt_3um_Std
Units: micrograms per liter (ug/L)
Description: <p>One standard deviation of Chl_a_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896347.rdf
Name: Chl_a_gt_7tenths_um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyl a in micrograms per liter in the size fraction representing the community greater than 0.7 micrometers (>0.7um); 0.000 = Below detection; Detection limit: 0.025 ug L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896348.rdf
Name: Chl_a_gt_7tenths_um_Std
Units: micrograms per liter (ug/L)
Description: <p>One standard deviation of Chl_a_gt_7tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896349.rdf
Name: Phaeo_gt_3um
Units: micrograms per liter (ug/L)
Description: <p>Phaeopigment in micrograms per liter in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896350.rdf
Name: Phaeo_gt_3um_Std
Units: micrograms per liter (ug/L)
Description: <p>One standard deviation of Phaeo_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896351.rdf
Name: Chl_a_gt_3tenths_um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyl a in micrograms per liter in the size fraction representing the community greater than 0.3 micrometers (>0.3um); 0.000 = Below detection; Detection limit: 0.025 ug L-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/896352.rdf
Name: Chl_a_gt_3tenths_um_Std
Units: micrograms per liter (ug/L)
Description: <p>One standard deviation of Chl_a_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896353.rdf
Name: Phaeo_gt_3tenths_um
Units: micrograms per liter (ug/L)
Description: <p>Phaeopigment in micrograms per liter in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896354.rdf
Name: Phaeo_gt_3tenths_um_Std
Units: micrograms per liter (ug/L)
Description: <p>One standard deviation of Phaeo_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896355.rdf
Name: Creatine_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate of creatine per hour in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896356.rdf
Name: Creatine_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of Creatine_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896357.rdf
Name: Creatine_V_3tenths_to_3um
Units: per hour
Description: <p>Specific uptake rate of creatine per hour in the size fraction representing the community between 0.3 and 3.0 micrometers (0.3-3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896358.rdf
Name: Creatine_V_3tenths_to_3um_Std
Units: per hour
Description: <p>One standard deviation of Creatine_V_3tenths_to_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896359.rdf
Name: Creatine_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate of creatine per hour in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896360.rdf
Name: Creatine_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of Creatine_V_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896361.rdf
Name: Isotope_Dilution_Corrected_Ammonium_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate per hour for ammonium, corrected for isotope dilution, in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896362.rdf
Name: Isotope_Dilution_Corrected_Ammonium_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of Isotope_Dilution_Corrected_Ammonium_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896363.rdf
Name: Isotope_Dilution_Corrected_Ammonium_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate per hour for ammonium, corrected for isotope dilution, in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896364.rdf
Name: Isotope_Dilution_Corrected_Ammonium_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of Isotope_Dilution_Corrected_Ammonium_V_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896365.rdf
Name: Ammonium_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate of ammonium per hour in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896366.rdf
Name: Ammonium_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of Ammonium_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896367.rdf
Name: Ammonium_V_3tenths_to_3um
Units: per hour
Description: <p>Specific uptake rate of ammonium per hour in the size fraction representing the community between 0.3 and 3.0 micrometers (0.3-3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896368.rdf
Name: Ammonium_V_3tenths_to_3um_Std
Units: per hour
Description: <p>One standard deviation of Ammonium_V_3tenths_to_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896369.rdf
Name: Ammonium_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate of ammonium per hour in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896370.rdf
Name: Ammonium_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of Ammonium_V_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896371.rdf
Name: Nitrate_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate of nitrate per hour in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896372.rdf
Name: Nitrate_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of Nitrate_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896373.rdf
Name: Nitrate_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate of nitrate per hour in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896374.rdf
Name: Nitrate_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of Nitrate_V_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896375.rdf
Name: Urea_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate of urea per hour in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896376.rdf
Name: Urea_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of Urea_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896377.rdf
Name: Urea_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate of urea per hour in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896378.rdf
Name: Urea_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of Urea_V_gt_3tenths_um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896379.rdf
Name: AA_V_gt_3um
Units: per hour
Description: <p>Specific uptake rate of amino acids per hour in the size fraction representing the community greater than 3.0 micrometers (>3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896380.rdf
Name: AA_V_gt_3um_Std
Units: per hour
Description: <p>One standard deviation of AA_V_gt_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896381.rdf
Name: AA_V_3tenths_to_3um
Units: per hour
Description: <p>Specific uptake rate of amino acids per hour in the size fraction representing the community between 0.3 and 3.0 micrometers (0.3-3.0um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896382.rdf
Name: AA_V_3tenths_to_3um_Std
Units: per hour
Description: <p>One standard deviation of AA_V_3tenths_to_3um</p>
http://lod.bco-dmo.org/id/dataset-parameter/896383.rdf
Name: AA_V_gt_3tenths_um
Units: per hour
Description: <p>Specific uptake rate of amino acids per hour in the size fraction representing the community greater than 0.3 micrometers (>0.3um)</p>
http://lod.bco-dmo.org/id/dataset-parameter/896384.rdf
Name: AA_V_gt_3tenths_um_Std
Units: per hour
Description: <p>One standard deviation of AA_V_gt_3tenths_um</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
26872
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/68797ddb-11e3-4ee7-a919-cb482933560c/content
download
https://doi.org/10.26008/1912/bco-dmo.896181.1
download
onLine
dataset
<p>Site water for incubation experiments and for the analysis of ambient nutrients was collected slightly differently for each of the sampling regions. In the Arctic, site water for incubation experiments was collected on the R/V Ukpik and R/V Sikuliaq (cruise ID number SK201712S) during the summer of 2017 using a submersible pump and CTD rosette, respectively. In Baja, sampling occurred twice on the R/V Robert Gordon Sproul during both May and October 2017 (cruise ID numbers SP1714 and SP1727), and site water was collected using a CTD rosette (Turk-Kubo et al. 2021). In Virginia, surface sites in the York River were sampled by directly filling an acid-washed PETG carboy over the side. Filtrations for sample filtrate and filters also varied. These York Rivers samples were obtained during day trips aboard a variety of small powerboats (&lt;21 feet), every other month from June 2018 to July 2019. Powerboats are housed out of the Virginia Institute of Marine Science.</p>
<p>In the Arctic, filtrations were conducted in parallel, which separately passed site water through a larger Nucleopore Membrane filter (3.0 micrometer (μm) nominal pore size) and a smaller Whatman GF-75 filter (nominal pore size 0.3 μm). This same combination of filters was used in Baja in October 2017. In May 2017, Baja sampling used the same filters, but filtered sequentially, meaning that site water was first passed through the Nucleopore filter before passing through the GF-75. The York River site samples were only filtered using the GF-75. Filtrate for all regions was collected from the GF-75 filtration and kept for later nutrient analyses and filters were used for chlorophyll <em>a </em>analysis.</p>
<p>Pigments (chlorophyll <em>a</em> and phaeopigments) were measured after extraction with 90% acetone overnight (Parsons et al. 1984; Arar and Collins 1997). Concentrations of ammonium were analyzed using the Koroleff (1983) method and amino acids were measured as dissolved primary amines (DPA; Parsons et al. 1984). Concentrations of nitrate, nitrite, phosphate, and silica were measured using a Lachat 8500 Quickchem autoanalyzer. Urea was analyzed using the monoxime method (Price and Harrison 1987). Concentrations of total dissolved nitrogen and dissolved organic carbon were assessed using a Shimadzu TOC-V TNM (Hansell 1993). Dissolved organic nitrogen is calculated as the difference between total dissolved nitrogen and inorganic nitrogen.&nbsp;</p>
<p>Nitrogen uptakes were measured after incubation for a set time (1 to 24 hours) in either 0.5 or 1-liter PETG bottles. Stable isotope tracer methods were used according to those described in Baer et al (2017). Uptake rates for inorganic and organic nitrogen were measured by incubating water with 15N- ammonium, nitrate, creatine, urea, and/or an amino acid mixture under <em>in situ</em> light and temperature conditions in a flow-through system on deck or in a cold room set to ambient site water temperatures. Rate incubation experiments were terminated with the same filtration methods as collection of site water ambients with the exception that a Sterlitech silver filter was used in lieu of the Nucleopore Membrane filter. The exact combination of nitrogen substrates varied between sites and regions. All nitrogen uptake samples were analyzed on a Sercon Integra 2 isotope ratio mass spectrometer.</p>
<p><strong>Known Issues/Problems:</strong><br />
Note that for the October sampling in the Baja region, chlorophyll <em>a</em> concentrations for the nutrient size fractions are unavailable. Instead, chlorophyll <em>a</em> concentrations for the &gt;0.7 μm size fraction are available thanks to the Arrigo and Zehr dataset (listed under "Related Datasets").</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
It is important to note that potential specific uptake rates are reported based on sampled size fraction (i.e. &gt;3.0 or &gt;0.3 μm). When applicable, the rates of different size fractions were combined for the final reported specific uptake rate (i.e &gt;3.0 and 0.3 - 3.0 μm fractions added to report a &gt;0.3 μm rate). Rates of ammonium-specific uptake were corrected for isotope dilution when possible. These rates were corrected according to Gilbert et al. (1982).</p>
<p><strong>BCO-DMO Processing:</strong><br />
- removed "NA" and "ND" (missing data values are blank/empty in the final .csv data file);<br />
- converted the Date column to YYYY-mm-dd format;<br />
- converted the Time column to HH:MM format;<br />
- created the ISO_DateTime_UTC column, which presents date and time in UTC in ISO 8601 format;<br />
- renamed fields to comply with BCO-DMO naming conventions in which the only allowed characters are letters (A-Z, a-z), numbers (0-9), and underscores.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sercon Integra2 Mass Spectrometer
Sercon Integra2 Mass Spectrometer
PI Supplied Instrument Name: Sercon Integra2 Mass Spectrometer PI Supplied Instrument Description:Used to measure isotope ratios and particulate masses Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
8-liter Niskin bottles
8-liter Niskin bottles
PI Supplied Instrument Name: 8-liter Niskin bottles PI Supplied Instrument Description:Used for site water sampling on R/V Sikuliaq and R/V Robert Gordon Sproul cruises Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Lachat QuickChem 8500 autoanalyzer
Lachat QuickChem 8500 autoanalyzer
PI Supplied Instrument Name: Lachat QuickChem 8500 autoanalyzer PI Supplied Instrument Description:Used to measure nitrate, nitrite, phosphate, and silicate concentrations Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Johnson Pump model # 16004
Johnson Pump model # 16004
PI Supplied Instrument Name: Johnson Pump model # 16004 PI Supplied Instrument Description:Used to collect site water samples on R/V Ukpik cruise Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Shimadzu 5000A TOC-V/TNM
Shimadzu 5000A TOC-V/TNM
PI Supplied Instrument Name: Shimadzu 5000A TOC-V/TNM PI Supplied Instrument Description:Used to measure dissolved organic carbon and total dissolved nitrogen concentrations Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
Shimadzu RF-600 Spectrofluorophotometer
Shimadzu RF-600 Spectrofluorophotometer
PI Supplied Instrument Name: Shimadzu RF-600 Spectrofluorophotometer PI Supplied Instrument Description:Used to measure concentrations of dissolved primary amines Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Turner Design Model 10-AU fluorometer
Turner Design Model 10-AU fluorometer
PI Supplied Instrument Name: Turner Design Model 10-AU fluorometer PI Supplied Instrument Description:Used to measure cholrophyll a and phaeopigement concentrations Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Shimadzu UV-1800 spectrophotometer
Shimadzu UV-1800 spectrophotometer
PI Supplied Instrument Name: Shimadzu UV-1800 spectrophotometer PI Supplied Instrument Description:Used to measure urea and ammonium concentrations Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: Ukpik_July-August_2017
Ukpik_July-August_2017
R/V Ukpik
R/V Ukpik
vessel
Ukpik_July-August_2017
Rachel E. Sipler
Cruise: SKQ201712S
SKQ201712S
R/V Sikuliaq
Community Standard Description
International Council for the Exploration of the Sea
R/V Sikuliaq
vessel
SKQ201712S
Laurie Juranek
Cruise: SP1714
SP1714
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel
SP1714
Matthew M. Mills
Stanford University
Cruise: SP1727
SP1727
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel
SP1727
Matthew M. Mills
Stanford University
R/V Ukpik
R/V Ukpik
vessel
R/V Sikuliaq
Community Standard Description
International Council for the Exploration of the Sea
R/V Sikuliaq
vessel
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel