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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/896385.rdf" xlink:actuate="onRequest">Louisiana shelf water column production and respiration measured by change in oxygen in light and dark bottle incubations during four cruises in 2017</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Lehrter, J., Fung, M., Hagemeyer, A. (2023) Louisiana shelf water column production and respiration measured by change in oxygen in light and dark bottle incubations during four cruises in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-05-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.896385.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Samples for plankton community respiration (WR) measurements were collected from several depths in the water column including the surface layer, the bottom layer, the layer at depth of chlorophyll maximum, midwater oxygen maximum, and midwater oxygen minimum. For each sample collected, three transparent (light) glass bottles and three dark glass bottles were filled directly from the ship's Niskin bottle array (Murrell and Lehrter, 2011). Aliquots were put into acid-cleaned 300 milliliter BOD (biochemical oxygen demand) bottles for light-dark incubation experiments. After initial O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;measurements were taken (T0), samples were placed in a darkened incubator that maintained surface water temperatures using water supplied continuously from the ship’s hull pump.&amp;amp;nbsp;Light bottle treatments were incubated in an outdoor incubator with screening that reduced ambient light by 50%. Dark bottle treatments were also placed in the outdoor incubator but were kept dark by their black liners and caps.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;For more detailed methods see Murrell et al. (2013) and Murrell and Lehrter (2011).&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Concentrations of O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; were measured initially and after 24 hours using a Hach or YSI oxygen sensor and temperature probe. Salinity&amp;amp;nbsp;values for each sample were obtained from the ship's CTD (SeaBird 911). Net primary production rates were calculated as the change in O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; over time in light bottles screened at 50% of ambient sunlight and incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; m&amp;lt;sup&amp;gt;-3&amp;lt;/sup&amp;gt; d&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;). Respiration rates were calculated as the change in O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; over time in dark bottles incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;m&amp;lt;sup&amp;gt;-3&amp;lt;/sup&amp;gt;&amp;amp;nbsp;d&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Problem report&amp;lt;/u&amp;gt;: Some of the incubation bottles had their caps come off during incubations. These values are labeled as NAN (not a number) in the dataset.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Note&amp;lt;/u&amp;gt;:&amp;amp;nbsp;Dissolved oxygen data were recorded in&amp;amp;nbsp;mg/L for the April 2017 cruise and in percent saturation for the July and&amp;amp;nbsp;September cruises.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/781475.rdf" xlink:title="OCE-1760747" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1760747 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1760747</gmx:Anchor>
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	Name: Cruise
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http://lod.bco-dmo.org/id/dataset-parameter/896562.rdf
	Name: Station
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http://lod.bco-dmo.org/id/dataset-parameter/896563.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/896564.rdf
	Name: Latitude
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http://lod.bco-dmo.org/id/dataset-parameter/896565.rdf
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	Units: decimal degrees
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http://lod.bco-dmo.org/id/dataset-parameter/896566.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/896567.rdf
	Name: Depth_Bottom
	Units: meters
	Description: &lt;p&gt;Depth of the bottom&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896568.rdf
	Name: Bottle_Number
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/896569.rdf
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	Units: meters
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http://lod.bco-dmo.org/id/dataset-parameter/896570.rdf
	Name: Salinity
	Units: psu
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http://lod.bco-dmo.org/id/dataset-parameter/896571.rdf
	Name: DO_Light_1_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Light bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896572.rdf
	Name: DO_Light_2_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Light bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896573.rdf
	Name: DO_Light_3_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Light bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896574.rdf
	Name: DO_Dark_1_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Dark bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896575.rdf
	Name: DO_Dark_2_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Dark bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896576.rdf
	Name: DO_Dark_3_T0
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Initial dissolved oxygen concentration in Dark bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896577.rdf
	Name: T_Light_1_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Light bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896578.rdf
	Name: T_Light_2_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Light bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896579.rdf
	Name: T_Light_3_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Light bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896580.rdf
	Name: T_Dark_1_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Dark bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896581.rdf
	Name: T_Dark_2_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Dark bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896582.rdf
	Name: T_Dark_3_T0
	Units: degrees Celsius
	Description: &lt;p&gt;Initial temperature of Dark bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896583.rdf
	Name: DO_Light_1_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Light bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896584.rdf
	Name: DO_Light_2_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Light bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896585.rdf
	Name: DO_Light_3_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Light bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896586.rdf
	Name: DO_Dark_1_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Dark bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896587.rdf
	Name: DO_Dark_2_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Dark bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896588.rdf
	Name: DO_Dark_3_TF
	Units: milligram per liter (mg/L) or percent saturation
	Description: &lt;p&gt;Final dissolved oxygen concentration in Dark bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896589.rdf
	Name: T_Light_1_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Light bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896590.rdf
	Name: T_Light_2_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Light bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896591.rdf
	Name: T_Light_3_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Light bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896592.rdf
	Name: T_Dark_1_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Dark bottle replicate 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896593.rdf
	Name: T_Dark_2_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Dark bottle replicate 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896594.rdf
	Name: T_Dark_3_TF
	Units: degrees Celsius
	Description: &lt;p&gt;Final temperature of Dark bottle replicate 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896595.rdf
	Name: CruiseStation
	Units: unitless
	Description: &lt;p&gt;Concatenated Cruise and Station&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896596.rdf
	Name: Time_in_local
	Units: unitless
	Description: &lt;p&gt;Incubation start time (local) when bottles were placed in the on-deck incubator&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/896597.rdf
	Name: Time_out_local
	Units: unitless
	Description: &lt;p&gt;Incubation end time (local) when bottles were taken out of the on-deck incubator&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;Samples for plankton community respiration (WR) measurements were collected from several depths in the water column including the surface layer, the bottom layer, the layer at depth of chlorophyll maximum, midwater oxygen maximum, and midwater oxygen minimum. For each sample collected, three transparent (light) glass bottles and three dark glass bottles were filled directly from the ship's Niskin bottle array (Murrell and Lehrter, 2011). Aliquots were put into acid-cleaned 300 milliliter BOD (biochemical oxygen demand) bottles for light-dark incubation experiments. After initial O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;measurements were taken (T0), samples were placed in a darkened incubator that maintained surface water temperatures using water supplied continuously from the ship’s hull pump.&amp;amp;nbsp;Light bottle treatments were incubated in an outdoor incubator with screening that reduced ambient light by 50%. Dark bottle treatments were also placed in the outdoor incubator but were kept dark by their black liners and caps.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;For more detailed methods see Murrell et al. (2013) and Murrell and Lehrter (2011).&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Concentrations of O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; were measured initially and after 24 hours using a Hach or YSI oxygen sensor and temperature probe. Salinity&amp;amp;nbsp;values for each sample were obtained from the ship's CTD (SeaBird 911). Net primary production rates were calculated as the change in O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; over time in light bottles screened at 50% of ambient sunlight and incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; m&amp;lt;sup&amp;gt;-3&amp;lt;/sup&amp;gt; d&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;). Respiration rates were calculated as the change in O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; over time in dark bottles incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;&amp;amp;nbsp;m&amp;lt;sup&amp;gt;-3&amp;lt;/sup&amp;gt;&amp;amp;nbsp;d&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt;).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Problem report&amp;lt;/u&amp;gt;: Some of the incubation bottles had their caps come off during incubations. These values are labeled as NAN (not a number) in the dataset.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Note&amp;lt;/u&amp;gt;:&amp;amp;nbsp;Dissolved oxygen data were recorded in&amp;amp;nbsp;mg/L for the April 2017 cruise and in percent saturation for the July and&amp;amp;nbsp;September cruises.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
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              <gmd:description>
                <gco:CharacterString>&amp;lt;p&amp;gt;&amp;lt;em&amp;gt;Please see the Supplemental Files section for equations in more easily readable format, along with a brief summary of the method for light and dark bottle oxygen assay to measure primary production and respiration&amp;lt;/em&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Net primary production, respiration, and gross primary production calculations&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Rates&amp;amp;nbsp;were adjusted to initial temperatures using the Arrhenius equation and initial and final temperatures.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Variables&amp;lt;/u&amp;gt;:&amp;lt;/p&amp;gt;

&amp;lt;ul&amp;gt;
&amp;lt;li&amp;gt;NPP is Net Primary Production&amp;amp;nbsp;&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;R is Respiration&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;GPP is&amp;amp;nbsp;Gross Primary Production&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;NPP_T is&amp;amp;nbsp;Temperature adjusted net primary production&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;R_T is&amp;amp;nbsp;Temperature adjusted respiration&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;GPP_T is&amp;amp;nbsp;Temperature adjusted gross primary production&amp;amp;nbsp;&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;DO_Light is the dissolved oxygen concentration in the light bottles&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;DO_Dark is the&amp;amp;nbsp;dissolved oxygen concentration in the dark bottles&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;TF = time final&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;T0 = time initial&amp;lt;/li&amp;gt;
&amp;lt;/ul&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;u&amp;gt;Equations&amp;lt;/u&amp;gt;:&amp;amp;nbsp; &amp;lt;em&amp;gt;(see Supplemental Files section)&amp;lt;/em&amp;gt;&amp;lt;br /&amp;gt;
NPP = (DO_Light_TF - DO_Light_T0)/(TF - T0)&amp;lt;br /&amp;gt;
R = (DO_Dark_TF - DO_Dark_T0)/(TF - T0)&amp;lt;br /&amp;gt;
GPP = NPP - R&amp;lt;br /&amp;gt;
NPP_T = NPP*2^((T_T0 - T_TF)./10)&amp;lt;br /&amp;gt;
R_T = R*2^((T_T0 - T_TF)./10)&amp;lt;br /&amp;gt;
GPP_T = GPP*2^((T_T0 - T_TF)./10)&amp;lt;br /&amp;gt;
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