http://lod.bco-dmo.org/id/dataset/898012
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-06-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Temperature-dependence of juvenile Black sea bass growth and lipid accumulation determined through lab experiments conducted from September 2021 to February 2022 at UConn Avery Point
2023-07-18
publication
2023-07-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-07-18
publication
https://doi.org/10.26008/1912/bco-dmo.898012.1
Hannes Baumann
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Zavell, M. D., Baumann, H. (2023) Temperature-dependence of juvenile Black sea bass growth and lipid accumulation determined through lab experiments conducted from September 2021 to February 2022 at UConn Avery Point. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-07-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.898012.1 [access date]
Temperature-dependence of juvenile Black sea bass growth and lipid accumulation - Experiment 2 Dataset Description: Methods and Sampling: <p>Juvenile Black sea bass (BSB) were caught on September 3rd (collection 1) and 21st 2021 (collection 2) via beach seine (30 × 2 meters) in Mumford Cove (41° 19' 25"N, 72° 01 '07"W), a shallow protected bay with extensive eelgrass cover in eastern Long Island Sound, USA.</p>
<p><strong>Experiment 2</strong> quantified juvenile winter growth and lipid accumulation under seasonally varying food and temperature conditions over the course of 182 days (October 1, 2021 to April 1, 2022). Initial total length (TL, mean ± SD = 70.9 ± 5.4 millimeters (mm)) and wet weight (wW, mean ± SD = 4.4 ± 0.9 grams (g)) were determined on briefly anesthetized specimens, followed by randomly distributing 48 juveniles into individual 20-liter (L) rearing containers with mesh-screened holes, an air stone, and a PVC hide. An additional baseline sample (n = 25, mean ± SD TL = 71.4 ± 9.4 mm, wW = 4.7 ± 1.8g) was euthanized and stored at -20° Celsius (C). We then used six 700 L recirculating tanks to each house eight individual BSB rearing containers (mean maximum stocking density = 0.13 kilograms per cubic meter (kg m⁻³). Tanks (n = 2 tanks per treatment; n = 8 containers per tank) were randomly assigned to one of three seasonally varying food treatments (Otohime C1 pellets) designed to simulate three phases of contrasting overwinter food availability for BSB juveniles. The 'Winter dip' treatment was fed 5% body wW 3× weekly during Phase 1 ("Fall": October, November), 2% wW 3× weekly during Phase 2 ("Winter": December – February) and again 5% body wW 3× weekly during Phase 3 ("Spring": March). This was to simulate a scenario of good onshore feeding conditions for juveniles in fall and spring, but poorer feeding conditions at their offshore overwintering habitats. Conversely, our 'Winter pulse' treatment was to simulate poor fall and spring feeding conditions for juveniles onshore by feeding 1% wW 3× weekly during Phase 1 and 3, but 2% wW during Phase 2, thereby simulating the same offshore winter feeding conditions (Fig.2A of Zavell et al. (in review)). Last, we included a 'Constant' treatment as control, which fed 2% wW 3× weekly throughout the entire six months of rearing (Phases 1-3). Food rations were based on both Cotton (2002), who showed no difference in growth at rations of 5 - 7.5% wW, but reduced growth at 2.5% wW and Watanabe et al. (2021) who report ideal feeding rates between 3 – 5% wW. For all treatments, rations were individually recalculated after the 1ˢᵗ of each month, based on re-measured wW and TL on briefly anesthetized fish.</p>
<p>Temperature conditions in Exp2 were recorded in 30 minute intervals using HOBO temperature loggers (Onset MX<sup>®</sup>).</p>
<p><strong>Response traits.</strong> Initial, monthly (Exp2 only), and final measurements of individual fish (TL, wW) were used to calculate total (final – initial), cumulative (end of month – initial), and/or serial (end of month – start of month) growth (e.g., cumulative and serial DTL for month 2 = TL<sub>d61</sub> – TL<sub>d0 </sub>and TL<sub>d61</sub> – TL<sub>d31</sub>), respectively) and average daily growth rates (growth/days in growth interval). Specific growth rates (SGR; % wW per day) were calculated similarly but used ln(wW) at each time period (e.g., 100*[ln(wW<sub>d61</sub>)-ln(wW<sub>d31</sub>)]). Growth efficiency (GE, %) was calculated cumulatively (Exp1, Exp2) and monthly (Exp2 only) as the change in body dry weight (DdW<sub>b</sub>, g) divided by total food consumed (DF, g) during a given time interval. (In Exp2, three GE values &gt; 100% were excluded as outliers). Q₁₀ values (Exp1) were calculated for GR, SGR, and GE between 6-12°C and 12-19°C.</p>
<p>For each experiment, we also determined the lipid, lean, and ash dry weights of each surviving BSB juvenile and those of the baseline samples. Whole specimens were first transferred to -80°C for one week, then freeze-dried at -50°C for one week and re-measured for whole body dry weight (dW<sub>b</sub>, 0.001 g). Dried specimens were then loaded into pre-weighed Alundum medium-porosity extraction thimbles and transferred into a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for a total of 3.5 hours to extract all metabolically accessible lipids (15-minute cycles of bathing, flushing, and ether replacement). After extraction, thimbles were dried overnight at 60°C and re-weighed to determine DdW, which equaled the total lipid content (dW<sub>Lipid</sub>, milligrams (mg)) of each specimen after accounting for any tissue loss during transfer from vial into thimble. Thimbles were then placed in a muffle furnace for 4 hours at 550°C and re-weighed, with DdW during this second step corresponding to a fish's lean mass (dW<sub>Lean</sub>, mg), again after accounting for any tissue loss during transfer from vial into thimble. The difference between the final weight and the pre-weighed empty thimble equaled ash (dW<sub>Ash</sub>, mg), i.e., the inorganic fraction of each individual.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536336 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536336
completed
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Site
Longitude
Latitude
Date_col
Species
Tank
BSB_ID
Group
Treat
Food
Date_sam
Exp_Phase
Month
TP
TL
TL0
DTL
wW
wW0
DwW
dW_est
dW0
DdW
GR_TP
GR_wW
GR_dW
Time
GRTL
SGRwW
SGRdW
Temp
TempSD
DD
Consum
GE
dW_final
TL_final
Lipid
Lean
Ash
Prop_Lipid
Prop_Lean
Prop_Ash
Automated Larval Fish Rearing System (ALFiRiS)
Custom-designed Soxhlet apparatus for Lipid/Lean analyses
HOBO temperature loggers (Onset)
theme
None, User defined
site
longitude
latitude
date
species scientific name (binomial)
tank
sample identification
sample description
treatment
month of year
time_point
length
weight
growth
days
water temperature at measurement depth
food consumption
lipids in biota
protein concentration
ash
featureType
BCO-DMO Standard Parameters
Automated Larval Fish Rearing System
Soxhlet extractor
Temperature Logger
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
https://osprey.bco-dmo.org/project/650184
Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
<p><em>Description from NSF award abstract:</em><br />
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.</p>
<p>This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.</p>
<p><strong>Related references:</strong><br />
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:<a href="http://dx.doi.org/10.1098/rsbl.2015.0976" target="_blank">10.1098/rsbl.2015.0976</a></p>
<p>Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:<a href="http://dx.doi.org/10.1002/loe2.10002" target="_blank">10.1002/loe2.10002</a></p>
<p>Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:<a href="http://dx.doi.org/10.3354/meps11142" target="_blank">10.3354/meps11142</a></p>
HYPOA
largerWorkCitation
project
eng; USA
oceans
-72.0019
-72.0019
41.3236
41.3236
2021-09-03
2022-04-01
Eastern Long Island Sound, CT, USA
0
BCO-DMO catalogue of parameters from Temperature-dependence of juvenile Black sea bass growth and lipid accumulation determined through lab experiments conducted from September 2021 to February 2022 at UConn Avery Point
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/904308.rdf
Name: Site
Units: unitless
Description: <p>Site name; Mumford Cove, CT - all fish</p>
http://lod.bco-dmo.org/id/dataset-parameter/904310.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of collection site in decimal degrees (negative values = West)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904312.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of collection site in decimal degrees (positive values = North)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904313.rdf
Name: Date_col
Units: unitless
Description: <p>Date of juvenile collection in the wild</p>
http://lod.bco-dmo.org/id/dataset-parameter/904314.rdf
Name: Species
Units: unitless
Description: <p>Name of species (Black Sea Bass - Centropristis striata)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904315.rdf
Name: Tank
Units: unitless
Description: <p>Experiment Tank (A - F), G represents Baseline Samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/904316.rdf
Name: BSB_ID
Units: unitless
Description: <p>ID of each BSB individual</p>
http://lod.bco-dmo.org/id/dataset-parameter/904317.rdf
Name: Group
Units: unitless
Description: <p>Experiment 2 OR Baseline (sacrificed at the beginning of the experiment)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904318.rdf
Name: Treat
Units: unitless
Description: <p>Seasonally varying food treatment, either 'Winter dip', 'Winter pulse', or 'Constant' | 'Baseline' denotes fish sampled at the beginning of the experiment (10/1/21; n=25) for initial lipid analyses. They were NOT reared and therefore have EMPTY fields for experimental parameters</p>
http://lod.bco-dmo.org/id/dataset-parameter/904319.rdf
Name: Food
Units: percent (%) wW per feeding
Description: <p>Mean realized food ration per feeding, based on offered food, consumed food per week, averaged over whole experiment, values of 3.8% feeding-1 ('Winter dip'), 1.7% feeding-1 ('Winter pulse'), and 2.2% feeding-1 ('Constant')</p>
http://lod.bco-dmo.org/id/dataset-parameter/904320.rdf
Name: Date_sam
Units: unitless
Description: <p>Date of repeated length and weight measurments</p>
http://lod.bco-dmo.org/id/dataset-parameter/904321.rdf
Name: Exp_Phase
Units: unitless
Description: <p>Phase i - iii of the experiment. Phase i was 'Fall' (Oct,Nov), Phase 'ii' was 'Winter' (Dec,Jan,Feb), Phase 'iii' was "Spring (Mar)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904322.rdf
Name: Month
Units: unitless
Description: <p>Month of length and weight measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/904323.rdf
Name: TP
Units: unitless
Description: <p>Time Point (values of 1 - 6)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904324.rdf
Name: TL
Units: millimeters (mm)
Description: <p>Total length at the end of the month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904325.rdf
Name: TL0
Units: millimeters (mm)
Description: <p>Total length at experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904326.rdf
Name: DTL
Units: millimeters (mm)
Description: <p>Change in TL from experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904327.rdf
Name: wW
Units: grams (g)
Description: <p>Wet Weight at the end of the month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904328.rdf
Name: wW0
Units: grams (g)
Description: <p>Wet Weight at experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904329.rdf
Name: DwW
Units: grams (g)
Description: <p>Change in wet Weight at experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904330.rdf
Name: dW_est
Units: grams (g)
Description: <p>Dry weight (estimated from post-mortem wet/dry weight relationship)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904331.rdf
Name: dW0
Units: grams (g)
Description: <p>Dry weight at experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904332.rdf
Name: DdW
Units: grams (g)
Description: <p>Change in dry weight from experiment start</p>
http://lod.bco-dmo.org/id/dataset-parameter/904333.rdf
Name: GR_TP
Units: millimeters (mm)
Description: <p>Growth in TL during month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904334.rdf
Name: GR_wW
Units: grams (g)
Description: <p>Growth wW during month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904335.rdf
Name: GR_dW
Units: grams (g)
Description: <p>Growth in dW during month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904336.rdf
Name: Time
Units: Days
Description: <p>Days of the month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904337.rdf
Name: GRTL
Units: millimeters per day (mm d-1)
Description: <p>Growth in TL per day</p>
http://lod.bco-dmo.org/id/dataset-parameter/904338.rdf
Name: SGRwW
Units: percent (%) wW per day
Description: <p>Weight-specific growth per day</p>
http://lod.bco-dmo.org/id/dataset-parameter/904339.rdf
Name: SGRdW
Units: percent (%) dW per day
Description: <p>Dry weight-specific growth per day</p>
http://lod.bco-dmo.org/id/dataset-parameter/904340.rdf
Name: Temp
Units: Degrees Celsius °C
Description: <p>Mean monthly temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/904341.rdf
Name: TempSD
Units: Degrees Celsius °C
Description: <p>Standard deviation of mean monthly temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/904342.rdf
Name: DD
Units: degree-days (°C × days)
Description: <p>Degree days this month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904343.rdf
Name: Consum
Units: grams (g)
Description: <p>Food consumed this month</p>
http://lod.bco-dmo.org/id/dataset-parameter/904344.rdf
Name: GE
Units: percent (%)
Description: <p>Gross growth efficiency (dryweight-based)</p>
http://lod.bco-dmo.org/id/dataset-parameter/904345.rdf
Name: dW_final
Units: milligrams (mg)
Description: <p>Dry weight measured after freeze-drying the specimen post-experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/904346.rdf
Name: TL_final
Units: millimeters (mm)
Description: <p>Final total length at the end of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/904347.rdf
Name: Lipid
Units: milligrams (mg)
Description: <p>Lipid content of the specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/904348.rdf
Name: Lean
Units: milligrams (mg)
Description: <p>Muscle/lean = protein content of the specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/904349.rdf
Name: Ash
Units: milligrams (mg)
Description: <p>Inorganic = ash content of the specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/904350.rdf
Name: Prop_Lipid
Units: percent (%)
Description: <p>Lipid / dW_final</p>
http://lod.bco-dmo.org/id/dataset-parameter/904351.rdf
Name: Prop_Lean
Units: percent (%)
Description: <p>Lean / dW_final</p>
http://lod.bco-dmo.org/id/dataset-parameter/904352.rdf
Name: Prop_Ash
Units: percent (%)
Description: <p>Ash / dW_final</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
70796
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/32a2b942-5b3e-4afb-8a63-606dc15439c5/content
download
https://doi.org/10.26008/1912/bco-dmo.898012.1
download
onLine
dataset
<p>Juvenile Black sea bass (BSB) were caught on September 3rd (collection 1) and 21st 2021 (collection 2) via beach seine (30 × 2 meters) in Mumford Cove (41° 19' 25"N, 72° 01 '07"W), a shallow protected bay with extensive eelgrass cover in eastern Long Island Sound, USA.</p>
<p><strong>Experiment 2</strong> quantified juvenile winter growth and lipid accumulation under seasonally varying food and temperature conditions over the course of 182 days (October 1, 2021 to April 1, 2022). Initial total length (TL, mean ± SD = 70.9 ± 5.4 millimeters (mm)) and wet weight (wW, mean ± SD = 4.4 ± 0.9 grams (g)) were determined on briefly anesthetized specimens, followed by randomly distributing 48 juveniles into individual 20-liter (L) rearing containers with mesh-screened holes, an air stone, and a PVC hide. An additional baseline sample (n = 25, mean ± SD TL = 71.4 ± 9.4 mm, wW = 4.7 ± 1.8g) was euthanized and stored at -20° Celsius (C). We then used six 700 L recirculating tanks to each house eight individual BSB rearing containers (mean maximum stocking density = 0.13 kilograms per cubic meter (kg m⁻³). Tanks (n = 2 tanks per treatment; n = 8 containers per tank) were randomly assigned to one of three seasonally varying food treatments (Otohime C1 pellets) designed to simulate three phases of contrasting overwinter food availability for BSB juveniles. The 'Winter dip' treatment was fed 5% body wW 3× weekly during Phase 1 ("Fall": October, November), 2% wW 3× weekly during Phase 2 ("Winter": December – February) and again 5% body wW 3× weekly during Phase 3 ("Spring": March). This was to simulate a scenario of good onshore feeding conditions for juveniles in fall and spring, but poorer feeding conditions at their offshore overwintering habitats. Conversely, our 'Winter pulse' treatment was to simulate poor fall and spring feeding conditions for juveniles onshore by feeding 1% wW 3× weekly during Phase 1 and 3, but 2% wW during Phase 2, thereby simulating the same offshore winter feeding conditions (Fig.2A of Zavell et al. (in review)). Last, we included a 'Constant' treatment as control, which fed 2% wW 3× weekly throughout the entire six months of rearing (Phases 1-3). Food rations were based on both Cotton (2002), who showed no difference in growth at rations of 5 - 7.5% wW, but reduced growth at 2.5% wW and Watanabe et al. (2021) who report ideal feeding rates between 3 – 5% wW. For all treatments, rations were individually recalculated after the 1ˢᵗ of each month, based on re-measured wW and TL on briefly anesthetized fish.</p>
<p>Temperature conditions in Exp2 were recorded in 30 minute intervals using HOBO temperature loggers (Onset MX<sup>®</sup>).</p>
<p><strong>Response traits.</strong> Initial, monthly (Exp2 only), and final measurements of individual fish (TL, wW) were used to calculate total (final – initial), cumulative (end of month – initial), and/or serial (end of month – start of month) growth (e.g., cumulative and serial DTL for month 2 = TL<sub>d61</sub> – TL<sub>d0 </sub>and TL<sub>d61</sub> – TL<sub>d31</sub>), respectively) and average daily growth rates (growth/days in growth interval). Specific growth rates (SGR; % wW per day) were calculated similarly but used ln(wW) at each time period (e.g., 100*[ln(wW<sub>d61</sub>)-ln(wW<sub>d31</sub>)]). Growth efficiency (GE, %) was calculated cumulatively (Exp1, Exp2) and monthly (Exp2 only) as the change in body dry weight (DdW<sub>b</sub>, g) divided by total food consumed (DF, g) during a given time interval. (In Exp2, three GE values &gt; 100% were excluded as outliers). Q₁₀ values (Exp1) were calculated for GR, SGR, and GE between 6-12°C and 12-19°C.</p>
<p>For each experiment, we also determined the lipid, lean, and ash dry weights of each surviving BSB juvenile and those of the baseline samples. Whole specimens were first transferred to -80°C for one week, then freeze-dried at -50°C for one week and re-measured for whole body dry weight (dW<sub>b</sub>, 0.001 g). Dried specimens were then loaded into pre-weighed Alundum medium-porosity extraction thimbles and transferred into a custom-designed Soxhlet apparatus, where they were bathed in petroleum ether for a total of 3.5 hours to extract all metabolically accessible lipids (15-minute cycles of bathing, flushing, and ether replacement). After extraction, thimbles were dried overnight at 60°C and re-weighed to determine DdW, which equaled the total lipid content (dW<sub>Lipid</sub>, milligrams (mg)) of each specimen after accounting for any tissue loss during transfer from vial into thimble. Thimbles were then placed in a muffle furnace for 4 hours at 550°C and re-weighed, with DdW during this second step corresponding to a fish's lean mass (dW<sub>Lean</sub>, mg), again after accounting for any tissue loss during transfer from vial into thimble. The difference between the final weight and the pre-weighed empty thimble equaled ash (dW<sub>Ash</sub>, mg), i.e., the inorganic fraction of each individual.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- Imported original file named "BCO-DMO-Source-BSB-Juvenile-Overwintering-V2.xlsx" sheet 3 into the BCO-DMO data system;<br />
- renamed fields to comply with BCO-DMO naming conventions (replaced spaces with underscores);<br />
- converted dates to YYYY-MM-DD format;<br />
- added columns for latitude and longitude in decimal degrees;<br />
- rounded values to 3 decimal places;<br />
- named the final file "898012_v1_bsb_experiment2.csv".</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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http://www.bco-dmo.org
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For questions regarding this resource, please contact BCO-DMO via the email address provided.
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Automated Larval Fish Rearing System (ALFiRiS)
Automated Larval Fish Rearing System (ALFiRiS)
PI Supplied Instrument Name: Automated Larval Fish Rearing System (ALFiRiS) Instrument Name: Automated Larval Fish Rearing System Instrument Short Name:ALFiRiS Instrument Description: The Automated Larval Fish Rearing System (ALFiRiS) was self-designed and assembled in the Rankin Lab at the University of Connecticut Avery Point. It consists of a 3 x 3 array of recirculating units (600 liters/150 gallons) that have independent computer-control over their temperature, oxygen, and pH conditions.
The system was designed to sequentially monitor tank conditions via industrial-grade oxygen and pH sensors (Hach) and then control gas solenoids (air, N2, CO2) to maintain and modulate environmental conditions. The system can apply static as well as fluctuating conditions on diel and tidal scales. Computerized temperature control allows simulating heatwaves and other non-static thermal regimes.
More information can be found at https://befel.marinesciences.uconn.edu/alfiris/
Custom-designed Soxhlet apparatus for Lipid/Lean analyses
Custom-designed Soxhlet apparatus for Lipid/Lean analyses
PI Supplied Instrument Name: Custom-designed Soxhlet apparatus for Lipid/Lean analyses Instrument Name: Soxhlet extractor Instrument Short Name: Instrument Description: A Soxhlet extractor is a piece of laboratory apparatus designed for the extraction of a lipid from a solid material. The solid is placed in a filter paper thimble which is then placed into the main chamber of the Soxhlet extractor. The solvent (heated to reflux) travels into the main chamber and the partially soluble components are slowly transferred to the solvent.
HOBO temperature loggers (Onset)
HOBO temperature loggers (Onset)
PI Supplied Instrument Name: HOBO temperature loggers (Onset) Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.