http://lod.bco-dmo.org/id/dataset/904358
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-07-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nitrate plus nitrite isotopic compositions from Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov from December 2016 to January 2017
2023-07-18
publication
2023-07-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-07-24
publication
https://doi.org/10.26008/1912/bco-dmo.904358.1
Angela N. Knapp
Florida State University
principalInvestigator
Sarah E. Fawcett
University of Cape Town
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Thomas, R. K., Knapp, A. N., Fawcett, S. E. (2023) Nitrate plus nitrite isotopic compositions from Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov from December 2016 to January 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-07-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.904358.1 [access date]
ACE-Leg 1 NO3 Isotopes Dataset Description: Methods and Sampling: <p>Samples were collected during Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov, which took place in the Indian Sector of the Southern Ocean from December 2016 to January 2017. Water column samples were collected by Niskin bottle on a CTD rosette. Surface samples were collected from the ship's underway intake (at ~5 meters depth). Nitrate and nitrite (NO3- + NO2-) d15N and d18O analyses were by the "denitrifier method" and followed the methods described by Sigman et al. (2001), Casciotti et al. (2002), McIlvin and Casciotti (2011), and Weigand et al. (2016). Briefly, NO3-+NO2- was quantitatively reduced to N2O by <em>Pseudomonas aureofaciens</em> and <em>Pseudomonas chlororaphis</em>, which was then cryogenically focused and analyzed on an isotope ratio mass spectrometer. A volume of sample was added to each bacterial vial to achieve a final quantity of 10 or 20 nanomoles (nmols) N2O, which was then purged from the vial using a helium carrier gas. The d15N and d18O of samples were calibrated with the international isotopic reference materials as described below.</p>
<p>The average precision of nitrate+nitrite d15N measurements was &lt;0.2 per mil and for d18O was &lt;0.3 per mil. NO3-+NO2- d15N and d18O analyses were calibrated with IAEA N3 and USGS 34 NO3- d15N isotopic reference materials as described in McIlvin and Casciotti (2011). NO3-+NO2- d18O was also calibrated with the USGS 35 isotopic reference material as described in McIlvin and Casciotti (2011).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851113 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851113
completed
Angela N. Knapp
Florida State University
(979) 862-5235
797 Lamar St. Dept of Oceanography, Eller O&M Room 1204
College Station
TX
77843
USA
aknapp@tamu.edu
pointOfContact
Sarah E. Fawcett
University of Cape Town
021 650 5369
Department of Oceanography 134 R.W. James Building
sarah.fawcett@uct.ac.za
pointOfContact
asNeeded
Dataset Version: 1
Unknown
CruiseID
Sampling_Date
Time_UTC
ISO_DateTime_UTC
Latitude
Longitude
STN
Cast
CastType
Depth
PotentialDensity
NO3_NO2_d15N
NO3_NO2_d18O_corrected
Thermo Delta V isotope ratio mass spectrometer
Niskin bottle on a CTD rosette
theme
None, User defined
Cruise identfier
date
time of day
ISO_DateTime_UTC
latitude
longitude
station
cast
cast type
depth
density
d15N
oxygen 16 to oxygen 18 ratio
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
ACE_Leg1
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Quantifying the effects of variable light and iron on the nitrate assimilation isotope effect of phytoplankton
https://osprey.bco-dmo.org/project/788969
Collaborative Research: Quantifying the effects of variable light and iron on the nitrate assimilation isotope effect of phytoplankton
<p><em>NSF Award Abstract:</em><br />
Phytoplankton are microscopic, single-celled organisms that play an important role in the Earth's ecosystems, elemental cycles, and climate. These organisms, which live in surface ocean waters, require sunlight and nutrients to grow and reproduce. In the oceans around Antarctica, nitrate (NO3-) as a nutrient source of nitrogen (N) is usually abundant while the nutrient iron is often sparse. Light availability also changes from complete darkness to 24 hours of constant sunlight, as well as from low light deeper in the water column to high, stressful light at the ocean surface. As a consequence, the phytoplankton in the Southern Ocean often live in a suboptimal environment in which conditions for growth are frequently changing. Scientists understand that nutrient supply and light availability affect these organisms and that these organisms, in turn, can alter the chemical composition of the seawater. For example, nitrate can occur in different forms, including a lighter (14N) and heavier (15N) form of NO3-, depending on which stable isotope of N is present in the molecule. Phytoplankton prefer to use the lighter isotope during uptake and incorporation into biomass, though the ratio of 15N/14N used by phytoplankton has been shown to vary depending on environmental conditions. Notably, the isotope ratio used by phytoplankton is recorded in sediments and can be used to determine both the historic composition of ocean waters and the productivity of phytoplankton. This project will test the hypothesis that enhanced light and/or iron stress change the isotopic ratios of water column nitrate- in specific ways. A combination of laboratory culture and field experiments will be conducted. Cultures of important Southern Ocean phytoplankton species will be grown under environmentally-relevant light and iron conditions where ratio of 15N/14N used by phytoplankton, physiological changes, and molecular markers of iron and light stress and nitrate assimilation will be measured. Similar measurements will be done in shipboard experiments on a cruise in the Southern Ocean with South African colleagues. These data will increase our understanding of past and present productivity in the Southern Ocean, and how phytoplankton changed the chemical composition of the seawater. Undergraduates from underrepresented groups in the STEM field and graduate students from Florida State University and Old Dominion University as well as students from South Africa will collaborate on this project. The improved process understanding of the N isotope effect will be presented not only at scientific national and international conferences but also during local outreach events at local K12 schools.</p>
<p>Interpretation of both modern water column nitrate (NO3-) isotopic ratio (d15N) measurements generated by GEOTRACES and other cruises, as well as metrics of paleo-nutrient utilization, depend upon a mechanistic understanding of the degree to which NO3- assimilation by phytoplankton discriminates against the heavier isotope, 15NO3- (NO3- assimilation epsilon). We currently lack the ability to predict how iron and light stress impacts the NO3- assimilation epsilon. The proposed work will test the hypothesis that enhanced light and/or iron stress elevates the epsilon for NO3-assimilation. This hypothesis will be tested by a combination of laboratory culture work and field work on a cruise of opportunity in the Southern Ocean. Mesocosm experiments will include both increasing and alleviating light and/or iron stress on monoclonal phytoplankton cultures and in natural phytoplankton communities while measuring the response of the NO3- assimilation epsilon. Water column samples will be collected on the cruise for analysis of dissolved and size-fractionated particulate N concentration and d15N, as well as phytoplankton community composition, photophysiology and gene expression markers of iron and light stress and NO3- assimilation. In particular, the expression of iron and light stress markers will be used to evaluate the relative contribution of iron and light stress to field-based estimates of the NO3-- assimilation epsilon. The results from these field measurements, together with lab-based culture studies, will be used to constrain the range of the epsilon for NO3- assimilation under environmentally-relevant light and iron conditions, including the potential alleviation of iron stress as has been hypothesized to have occurred during the last glacial maximum (a.k.a. the Martin hypothesis).</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Nitrate Assimilation Phytoplankton
largerWorkCitation
project
eng; USA
oceans
37.63233
141.9254
-55.116
-46.0848
2016-12-26
2017-01-17
Southern Atlantic Ocean, Southern Ocean, laboratory experiments at FSU
0
BCO-DMO catalogue of parameters from Nitrate plus nitrite isotopic compositions from Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov from December 2016 to January 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/904400.rdf
Name: CruiseID
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/904401.rdf
Name: Sampling_Date
Units: unitless
Description: <p>Date on which the sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/904402.rdf
Name: Time_UTC
Units: unitless
Description: <p>Time (UTC) at which the sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/904403.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time (UTC) of sample collection in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/904404.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude at which the sample was collected; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/904405.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude at which the sample was collected; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/904406.rdf
Name: STN
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/904407.rdf
Name: Cast
Units: unitless
Description: <p>Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/904408.rdf
Name: CastType
Units: unitless
Description: <p>Type of cast: UDW (underway) or CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/904409.rdf
Name: Depth
Units: meters
Description: <p>Depth at which the sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/904410.rdf
Name: PotentialDensity
Units: kilograms per cubic meter, with reference to sea surface (kg m-3)
Description: <p>Potential density of depth at which sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/904411.rdf
Name: NO3_NO2_d15N
Units: per mil vs. N2 in air
Description: <p>Nitrogen isotopic composition of nitrate+nitrite in a sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/904412.rdf
Name: NO3_NO2_d18O_corrected
Units: per mil vs. VSMOW
Description: <p>Oxygen isotopic composition of nitrate+nitrite in a sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
18270
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ec4e2396-606f-4530-b68f-8c45ba8a6a57/content
download
https://doi.org/10.26008/1912/bco-dmo.904358.1
download
onLine
dataset
<p>Samples were collected during Leg 1 of the Antarctic Circumnavigation Expedition (ACE) research cruise onboard the R/V Akademik Treshnikov, which took place in the Indian Sector of the Southern Ocean from December 2016 to January 2017. Water column samples were collected by Niskin bottle on a CTD rosette. Surface samples were collected from the ship's underway intake (at ~5 meters depth). Nitrate and nitrite (NO3- + NO2-) d15N and d18O analyses were by the "denitrifier method" and followed the methods described by Sigman et al. (2001), Casciotti et al. (2002), McIlvin and Casciotti (2011), and Weigand et al. (2016). Briefly, NO3-+NO2- was quantitatively reduced to N2O by <em>Pseudomonas aureofaciens</em> and <em>Pseudomonas chlororaphis</em>, which was then cryogenically focused and analyzed on an isotope ratio mass spectrometer. A volume of sample was added to each bacterial vial to achieve a final quantity of 10 or 20 nanomoles (nmols) N2O, which was then purged from the vial using a helium carrier gas. The d15N and d18O of samples were calibrated with the international isotopic reference materials as described below.</p>
<p>The average precision of nitrate+nitrite d15N measurements was &lt;0.2 per mil and for d18O was &lt;0.3 per mil. NO3-+NO2- d15N and d18O analyses were calibrated with IAEA N3 and USGS 34 NO3- d15N isotopic reference materials as described in McIlvin and Casciotti (2011). NO3-+NO2- d18O was also calibrated with the USGS 35 isotopic reference material as described in McIlvin and Casciotti (2011).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- imported original file named "Thomas_ACE_NO3d15N_d18O_CTD_UDW.xlsx" into the BCO-DMO system;<br />
- marked "no data" as a missing data value (missing data are blank/empty in the final csv file);<br />
- renamed fields to comply with BCO-DMO naming conventions;<br />
- converted Sampling_Date column to %Y-%m-%d format;<br />
- added the ISO 8601 date-time column (UTC);<br />
- named the final file "904358_v1_ace_leg1_no3_isotopes.csv".</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Delta V isotope ratio mass spectrometer
Thermo Delta V isotope ratio mass spectrometer
PI Supplied Instrument Name: Thermo Delta V isotope ratio mass spectrometer PI Supplied Instrument Description:Nitrate+nitrite d15N and d18O was measured using a Thermo Delta V isotope ratio mass spectrometer. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Niskin bottle on a CTD rosette
Niskin bottle on a CTD rosette
PI Supplied Instrument Name: Niskin bottle on a CTD rosette Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: ACE_Leg1
ACE_Leg1
Community Standard Description
International Council for the Exploration of the Sea
R/V Akademik Tryoshnikov
vessel
ACE_Leg1
David Walton
Community Standard Description
International Council for the Exploration of the Sea
R/V Akademik Tryoshnikov
vessel