http://lod.bco-dmo.org/id/dataset/904895
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-07-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Element quotas of individual phytoplankton cells from samples collected on the US GEOTRACES Arctic cruise GN01 (HLY1502) on USCGC Healy in August-October 2015
2025-06-09
publication
2025-06-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-09-05
publication
https://doi.org/10.26008/1912/bco-dmo.904895.1
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Twining, B. (2025) Element quotas of individual phytoplankton cells from samples collected on the US GEOTRACES Arctic cruise GN01 (HLY1502) on USCGC Healy in August-October 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2025-06-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.904895.2 [access date]
GN01 Element quotas of individual phytoplankton cells Dataset Description: Methods and Sampling: <p>Single-cell SXRF samples were collected from the surface mixed layer using the GEOTRACES rosette. Whole water samples were preserved with 0.25% trace-metal clean buffered glutaraldehyde (Twining et al., 2003) and centrifuged onto Au or SiN TEM windows. Grids were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011). Each cell was raster scanned with a focused 10 keV x-ray beam with a diameter of approximately 0.5 micrometers (µm).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1435862 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1435862
completed
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive PO Box 380
East Boothbay
ME
04544-0380
USA
btwining@bigelow.org
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Run
Cruise
Station
Lat_N
Lon_E
Date
Start_time
ISO_DateTime_UTC
Depth
DepthDescr
GEOTRC_SAMPNO
CellType
MDA
UniqueCell
GridType
Volume
cellC
cellSi
cellP
cellS
cellMn
cellFe
cellCo
cellNi
cellCu
cellZn
cell_image_file
spectral_file
maps_file
Synchrotron X-ray Fluorescence microscope
theme
None, User defined
experiment id
Cruise identfier
station
latitude
longitude
date
time of day
ISO_DateTime_UTC
depth
depth nominal
sample identification
sample description
sampling_method
volume
Elemental carbon (C)
Silicon
Phosphorus
Sulfur (S)
Manganese
Iron
Cobalt
Nickel
Copper
Zinc
file_name
featureType
BCO-DMO Standard Parameters
X-ray fluorescence analyzer
instrument
BCO-DMO Standard Instruments
HLY1502
service
Deployment Activity
Western Arctic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
U.S. Arctic GEOTRACES Study (GN01)
https://www.geotraces.org/
U.S. Arctic GEOTRACES Study (GN01)
<p><em>Description from NSF award abstract:</em><br />
In pursuit of its goal "to identify processes and quantify fluxes that control the distributions of key trace elements and isotopes in the ocean, and to establish the sensitivity of these distributions to changing environmental conditions", in 2015 the International GEOTRACES Program will embark on several years of research in the Arctic Ocean. In a region where climate warming and general environmental change are occurring at amazing speed, research such as this is important for understanding the current state of Arctic Ocean geochemistry and for developing predictive capability as the regional ecosystem continues to warm and influence global oceanic and climatic conditions. The three investigators funded on this award, will manage a large team of U.S.scientists who will compete through the regular NSF proposal process to contribute their own unique expertise in marine trace metal, isotopic, and carbon cycle geochemistry to the U.S. effort. The three managers will be responsible for arranging and overseeing at-sea technical services such as hydrographic measurements, nutrient analyses, and around-the-clock management of on-deck sampling activites upon which all participants depend, and for organizing all pre- and post-cruise technical support and scientific meetings. The management team will also lead educational outreach activities for the general public in Nome and Barrow, Alaska, to explain the significance of the study to these communities and to learn from residents' insights on observed changes in the marine system. The project itself will provide for the support and training of a number of pre-doctoral students and post-doctoral researchers. Inasmuch as the Arctic Ocean is an epicenter of global climate change, findings of this study are expected to advance present capability to forecast changes in regional and globlal ecosystem and climate system functioning.</p>
<p>As the United States' contribution to the International GEOTRACES Arctic Ocean initiative, this project will be part of an ongoing multi-national effort to further scientific knowledge about trace elements and isotopes in the world ocean. This U.S. expedition will focus on the western Arctic Ocean in the boreal summer of 2015. The scientific team will consist of the management team funded through this award plus a team of scientists from U.S. academic institutions who will have successfully competed for and received NSF funds for specific science projects in time to participate in the final stages of cruise planning. The cruise track segments will include the Bering Strait, Chukchi shelf, and the deep Canada Basin. Several stations will be designated as so-called super stations for intense study of atmospheric aerosols, sea ice, and sediment chemistry as well as water-column processes. In total, the set of coordinated international expeditions will involve the deployment of ice-capable research ships from 6 nations (US, Canada, Germany, Sweden, UK, and Russia) across different parts of the Arctic Ocean, and application of state-of-the-art methods to unravel the complex dynamics of trace metals and isotopes that are important as oceanographic and biogeochemical tracers in the sea.</p>
U.S. GEOTRACES Arctic
largerWorkCitation
project
GEOTRACES Arctic Section: Collaborative Research: Biogeochemical cycling of particulate trace elements in the western Arctic basin
https://osprey.bco-dmo.org/project/653218
GEOTRACES Arctic Section: Collaborative Research: Biogeochemical cycling of particulate trace elements in the western Arctic basin
<p><em>NSF Award Abstract:</em><br />
As part of this project, two investigators will participate in the 2015 U.S. GEOTRACES Arctic expedition to determine the trace element composition of different suspended particles in the water column of the Arctic Ocean to identify the sources and scavenging capabilities of these particles. In common with other multinational initiatives in the International GEOTRACES Program, the goals of the U.S. Arctic expedition are to identify processes and quantify fluxes that control the distributions of key trace elements and isotopes in the ocean, and to establish the sensitivity of these distributions to changing environmental conditions. Some trace elements are essential to life, others are known biological toxins, and still others are important because they can be used as tracers of a variety of physical, chemical, and biological processes in the sea. This team of trace element scientists will focus on the measurement of particulate trace elements, which will enable scientists to better estimate the sources of particulate metals to the Arctic Ocean and to better understand their contribution to biological processes. This project will also provide educational opportunities for undergraduate students, K-12 teachers, and the general public.</p>
<p>Particulate trace element distributions, sources, sinks and cycling are predicted to be controlled by physical, biological, anthropogenic and geochemical processes in the Arctic. Furthermore, many trace elements are required nutrients for marine phytoplankton, playing a key role in oceanic primary productivity. However, few integrated oceanographic studies have been conducted to specifically investigate these relationships in this region, despite the changing conditions and global significance of the Arctic. This project will significantly advance understanding of the complete geochemical cycles of a number of trace elements in the Arctic Ocean basin by measuring the concentrations of particulate trace elements in bulk particles collected through the water column and in sediments, as well as in phytoplankton from the upper water column. These measurements will enable scientists to constrain the inputs of particulate elements from rivers, shelves and ice, and to assess the removal of dissolved trace elements via passive scavenging and biological uptake.</p>
Arctic GEOTRACES bottle particles
largerWorkCitation
project
eng; USA
oceans
Western Arctic Ocean
-149.43
89.25
60.26
89.99
2015-08-12
2015-10-05
From projects that focused on the following 2 locations: 1. Arctic Ocean; Sailing from Dutch Harbor to Dutch Harbor (GN01) 2. Arctic Ocean
0
BCO-DMO catalogue of parameters from Element quotas of individual phytoplankton cells from samples collected on the US GEOTRACES Arctic cruise GN01 (HLY1502) on USCGC Healy in August-October 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/907753.rdf
Name: Run
Units: unitless
Description: <p>APS run during which each cell was analyzed: 2016r1 or 2016r3 (runs 1 and 3, respectively)</p>
http://lod.bco-dmo.org/id/dataset-parameter/907754.rdf
Name: Cruise
Units: unitless
Description: <p>GEOTRACES cruise the sample was collected from: Arctic (GN01, HLY02)</p>
http://lod.bco-dmo.org/id/dataset-parameter/907755.rdf
Name: Station
Units: unitless
Description: <p>Station sample was collected from</p>
http://lod.bco-dmo.org/id/dataset-parameter/907756.rdf
Name: Lat_N
Units: degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/907757.rdf
Name: Lon_E
Units: degrees East
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/907758.rdf
Name: Date
Units: unitless
Description: <p>Date of cast (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/907759.rdf
Name: Start_time
Units: unitless
Description: <p>Time of cast start (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/907760.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of cast start (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/907761.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/907762.rdf
Name: DepthDescr
Units: unitless
Description: <p>Descriptive depth designation: ML (mixed layer) or in ice</p>
http://lod.bco-dmo.org/id/dataset-parameter/907763.rdf
Name: GEOTRC_SAMPNO
Units: unitless
Description: <p>GEOTRACES sample ID for particulate trace metal sample from which phytoplankton were sampled; event numbers are given if no sample number was assigned.</p>
http://lod.bco-dmo.org/id/dataset-parameter/907764.rdf
Name: CellType
Units: unitless
Description: <p>Taxonomic classification of cell: Chaet = chaetoceros, centric = centric diatom, Cosco = coscinodiscus, Dino = dinoflagellage, flag = flagellate, Melosira, Oval diatom, pennate = pennate diatom, Silicaflag = silicaflagellate</p>
http://lod.bco-dmo.org/id/dataset-parameter/907765.rdf
Name: MDA
Units: unitless
Description: <p>SXRF scan number</p>
http://lod.bco-dmo.org/id/dataset-parameter/907766.rdf
Name: UniqueCell
Units: unitless
Description: <p>Unique cell ID, formatted as {RUN}_{MDA}_{ROI}</p>
http://lod.bco-dmo.org/id/dataset-parameter/907767.rdf
Name: GridType
Units: unitless
Description: <p>Material of TEM grid used for analysis: SiN (silicon nitride) or Au (gold)</p>
http://lod.bco-dmo.org/id/dataset-parameter/907768.rdf
Name: Volume
Units: cubic microns
Description: <p>Cell volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/907769.rdf
Name: cellC
Units: moles
Description: <p>cellular carbon contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907770.rdf
Name: cellSi
Units: moles
Description: <p>cellular silicon contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907771.rdf
Name: cellP
Units: moles
Description: <p>cellular phosphorus contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907772.rdf
Name: cellS
Units: moles
Description: <p>cellular sulfur contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907773.rdf
Name: cellMn
Units: moles
Description: <p>cellular manganese contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907774.rdf
Name: cellFe
Units: moles
Description: <p>cellular iron contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907775.rdf
Name: cellCo
Units: moles
Description: <p>cellular cobalt contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907776.rdf
Name: cellNi
Units: moles
Description: <p>cellular nickel contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907777.rdf
Name: cellCu
Units: moles
Description: <p>cellular copper contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907778.rdf
Name: cellZn
Units: moles
Description: <p>cellular zinc contents</p>
http://lod.bco-dmo.org/id/dataset-parameter/907779.rdf
Name: cell_image_file
Units: unitless
Description: <p>filename of light microscope image of cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/907780.rdf
Name: spectral_file
Units: unitless
Description: <p>filename of SXRF spectrum of cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/907781.rdf
Name: maps_file
Units: unitless
Description: <p>filename of 2D heatmap of elemental contents of cell</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
50420
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4fc0de59-7340-4cdd-9472-ebbcbf1ab08a/content
download
74485
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/2ece06ca-284d-4e03-a1b1-d2c85e5d5a0a/content
download
https://doi.org/10.26008/1912/bco-dmo.904895.1
download
onLine
dataset
<p>Single-cell SXRF samples were collected from the surface mixed layer using the GEOTRACES rosette. Whole water samples were preserved with 0.25% trace-metal clean buffered glutaraldehyde (Twining et al., 2003) and centrifuged onto Au or SiN TEM windows. Grids were briefly rinsed with a drop of ultrapure water and dried in a Class-100 cabinet. SXRF analysis was performed using the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory) following the protocols of Twining et al. (2011). Each cell was raster scanned with a focused 10 keV x-ray beam with a diameter of approximately 0.5 micrometers (µm).</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
Fluorescence spectra from the pixels covering the cell were averaged to calculate whole-cell quotas, and a fluorescence spectrum from a neighboring empty section of the grid was subtracted. Cellular elemental fluorescence intensities were fit with a modified-Gaussian model using custom software and peak areas converted to areal element concentrations using NBS-certified standard reference materials (Núnez-Milland et al., 2010; Twining et al., 2011). Spatial regions of interest (ROI) representing the whole cell (including any adsorbed elements, if present) were prepared for each cell and used to calculate element quotas. Cellular C quotas were calculated from cell biovolume using the equations of Menden-Deuer and Lessard (2000). Cell biovolume was calculated for each cell from measurements of cell diameter, length, and height using digital image processing software Image J. Shape and volume equations were taken from Hillebrand et al. (1999).</p>
Specified by the Principal Investigator(s)
Version 1:
Version date: 2023-09-05
Description: The original version of the dataset published at BCO-DMO.
- Imported original file named "Arctic SXRF.csv" into the BCO-DMO system;
- Removed "NA" as a missing data value (missing data is blank/empty in the final CSV file);
- Replaced spaces with underscores in column names to comply with BCO-DMO naming conventions;
- Changed Date format to YYYY-mm-dd;
- Added the date-time field in ISO 8601 format;
- Named the final file "904895_v1_gn01_phytoplankton_element_quotas.csv".
Version 2:
Version date: 2025-06-09
Description: This version includes corrections to station, lat, long, and times for event 6196; corrected depth for sample number 10490; and the removal of duplicate rows.
- Imported PI-modified file named "904895_v1_gn01_phytoplankton_element_quotas_5June2025update.xlsx" into the BCO-DMO system.
- Converted Date format to %Y-%m-%d.
- Saved the final file as "904895_v2_gn01_phytoplankton_element_quotas.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Synchrotron X-ray Fluorescence microscope
Synchrotron X-ray Fluorescence microscope
PI Supplied Instrument Name: Synchrotron X-ray Fluorescence microscope PI Supplied Instrument Description:SXRF analysis was performed on the 2-ID-E beamline at the Advanced Photon source (Argonne National Laboratory). The synchrotron consists of a storage ring which produces high energy electromagnetic radiation. X-rays diverted to the 2-ID-E beamline are used for x-ray fluorescence mapping of biological samples. X-rays were tuned to an energy of 10 keV to enable the excition of K-alpha fluorescence for the elements reported. The beam is focused using Fresnel zoneplates to achieve high spatial resolution; for our application a focused spot size of 0.5um was used. A multi-element germanium energy dispersive detector is used to record the X-ray fluorescence spectrum. Instrument Name: X-ray fluorescence analyzer Instrument Short Name:XRF analyzer Instrument Description: Instruments that identify and quantify the elemental constituents of a sample from the spectrum of electromagnetic radiation emitted by the atoms in the sample when excited by X-ray radiation.
Cruise: HLY1502
HLY1502
USCGC Healy
Community Standard Description
International Council for the Exploration of the Sea
USCGC Healy
vessel
HLY1502
David C. Kadko
Florida International University
https://datadocs.bco-dmo.org/docs/302/geotraces/GEOTRACES_ARCTIC/data_docs/cruise_reports/healy1502.pdf
Report describing HLY1502
USCGC Healy
Community Standard Description
International Council for the Exploration of the Sea
USCGC Healy
vessel