http://lod.bco-dmo.org/id/dataset/904962 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-07-21 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻi 2023-07-21 publication 2023-07-21 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-11-28 publication https://doi.org/10.26008/1912/bco-dmo.904962.1 Katie Barott University of Pennsylvania principalInvestigator Hollie Putnam University of Rhode Island principalInvestigator Kristen Brown University of Pennsylvania principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Barott, K., Putnam, H., Brown, K. T. (2023) Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻi. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-07-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.904962.1 [access date] Dataset Description: &lt;p&gt;This dataset and other data from this study will be published in the results paper &quot;Divergent bleaching and recovery trajectories in reef-building corals following a decade of successive marine heatwaves.&quot; (see pre-print Brown, et al. (2023), doi: 10.1101/2023.07.16.549193).&amp;nbsp;&lt;br /&gt; &lt;br /&gt; All BCO-DMO datasets related to this publication can be found on the page https://www.bco-dmo.org/related-resource/915300.&lt;/p&gt; Methods and Sampling: &lt;p&gt;Location:&amp;nbsp;&lt;br /&gt; patch reef 13 in Kāne'ohe Bay, O'ahu, Hawai'i (21.4509, -157.7954)&amp;nbsp;&lt;br /&gt; &lt;br /&gt; Physiological analyses:&lt;/p&gt; &lt;p&gt;A total of 276 fragments were collected from colonies of bleaching-resistant and bleaching-susceptible M. capitata and P. compressa (n= 7–10 pairs per phenotype per species) across eight timepoints from October 2019 to September 2022, incorporating the peak of the 2019 heatwave and three years (35 months) of recovery during non-heatwave years (Fig. S1,&amp;nbsp;Brown et al. (2023)). Fragments (4–5 cm in length) were collected by hand using bone cutters, transported to the Hawai’i Institute of Marine Biology (HIMB) in ambient seawater and held in flow-through seawater aquaria for 24–72 hours until measurements of coral performance. For four timepoints (October 2019, October 2021, March 2022, and September 2022), metabolic rates were assessed via changes in oxygen evolution using oxygen optodes (PSt7, PreSens) connected to an optical analyser (OXY-10, PreSens) (Innis et al., 2021). Oxygen optodes were calibrated on each day with a 0% oxygen solution (0.01 g ml−1 NaSO3) and 100% air saturated seawater. Coral fragments were analyzed between 08:00 and 17:00 within 250 ml clear acrylic chambers on top of a magnetic stirrer to allow for continuous mixing. Seawater temperatures were replicated to those experienced on the reef by using ambient seawater and a water jacket to maintain temperatures within the incubation chambers. Temperature and dissolved oxygen concentrations were recorded every 3 seconds at increasing increments of light over a total of 45–50 minutes to determine maximum net photosynthesis (Pmax). Light levels ranged from 112–726 µmol m-2 sec-1 in October 2019 &amp;nbsp;(Innis et al., 2021) (step size ~100 µmol m-2 sec-1; ~10 minutes step-1), and from 387–1800 µmol m-2 sec-1 in 2020–2021 (step size ~300 µmol m-2 sec-1; ~10 minutes step-1). After measurements were completed at the maximum light levels, the lights were turned off (0 µmol m-2 sec-1) to measure light-enhanced dark respiration (LEDR) as defined by (Edmunds and&amp;nbsp;Davies, 1988). Photosynthesis-irradiance curves were fitted using the Platt model to extract Pmax and LEDR (Innis et al., 2021; Platt et al., 1981 ), 3). Upon completion of these in vivo analyses, coral fragments were flash frozen in liquid nitrogen and stored at −80°C until further processing.&lt;/p&gt; &lt;p&gt;Coral tissues containing symbionts were removed from skeletons with a waterpik using 50 mL of 0.1 M phosphate buffered saline (PBS) solution. The resulting holobiont homogenate was centrifuged at 4°C for 5 minutes at 2500 x g to separate the coral host fraction (supernatant) from the intracellular endosymbiont cells (family Symbiodiniaceae; pellet). Symbiodiniaceae were resuspended in PBS and cell abundances were quantified using a Millipore Guava flow-cytometer (Guava easyCyte 5HT) following the methodology of (Innis et al., 2021). Symbiodiniaceae cells were excited with a blue laser (488 nm) and identified by analyzing forward scatter and red autofluorescence in GuavaSoft 3.4 with the same gating for all samples. Symbiont densities were standardized to skeletal surface area (cm2), which was determined using the wax-dipping technique (Holmes, 2008;&amp;nbsp;Stimson and&amp;nbsp;Kinzie, 1991). Endosymbiont photopigments were extracted in 100% acetone for 24 hours and the concentration of chlorophyll-a was determined via absorbance at 630, 663, and 750 nm using the equation in (Jeffrey and Humphrey, 1975) and subtracting the absorbance at 750 nm from both A663 and A630:&amp;nbsp;&lt;/p&gt; &lt;p&gt;Chlorophyll a =(11.43 (A663-A750)) -(0.64 (A630-A750))&lt;/p&gt; &lt;p&gt;Photopigment concentrations were standardized to both skeletal surface area and symbiont densities. Endosymbiont community composition (proportion Durusdinium vs. Cladocopium) of M. capitata colonies was determined by Dilworth et al. 2021 from samples collected in July 2019 using quantitative PCR.&amp;nbsp;&lt;/p&gt; &lt;p&gt;Host tissue biomass was determined as ash-free dry weight (AFDW) from the coral fraction (i.e. symbionts removed as described above). First, 1 mL of the coral fraction was dried at 60°C for 24 hours until a constant weight was achieved. After the dry weight was recorded, the samples were then burned in a muffle furnace at 450°C for 6 hours. The samples were allowed to cool in the furnace before being weighed and the weight of the resulting ash was recorded. The difference between the ash weight and the dry weight was calculated to determine the AFDW of each sample. Host soluble protein content of each sample was determined via the Bradford method. Specifically, 10 μL of the coral fraction was pipetted into each of three wells of a 96-well plate (technical replicates), followed by the addition of 300 μL of Coomassie Plus Bradford reagent (Thermo Fisher Scientific) to each well. The plate was incubated for 10 minutes at room temperature followed by the collection of an absorbance reading at 595 nm on a spectrophotometer (BioTek PowerWave XS2). Each plate contained a set of internal standards with known concentrations of bovine serum albumin (0–2000 μg mL-1), which were used to generate a second-degree polynomial standard curve relating absorbance (x) to protein concentration (y). The standard curve equation was used to calculate the protein concentrations of the samples. The concentration of lipids in each sample was determined via a modified method of (Dunn et al., 2012). First, ~45 mL of the coral fraction was lyophilized to produce dry tissue. A subsample of the lyophilized tissue was weighed (~90 mg), and 2 mL of chloroform-methanol (2:1) was added. The sample was then mixed using a homogenizer at 15000 rpm for 5 seconds, and an additional 2 mL of chloroform-methanol was used to rinse the homogenizer into the sample, and the tubes were then vigorously vortexed before being placed at 4°C for 2 hours in the dark. After incubation, tubes were again vortexed and the chilled homogenates were then passed through 0.22 μm syringe filters into new pre-weighed tubes. An additional 1 mL of chloroform-methanol was passed through the filters into each tube to ensure the full passage of sample material. Next, 1 mL of 0.1 M KCl was added to the samples, which were then vortexed and placed at 4°C for at least 1 hour in the dark until two phases formed. The aqueous (top) phase was discarded, and 5 mL of 50% methanol was added to the organic (bottom) phase. The samples were then placed at 4°C for 1 hour in the dark, followed by removal of the aqueous (top) phase. Washes with 50% methanol were repeated twice more, and the remaining organic phases were dried under a fume hood until the solvent had completely evaporated, at which point the lipid pellet was weighed. Coral host biomass, protein and lipid content were standardized to skeletal surface area.&amp;nbsp;&lt;/p&gt; &lt;p&gt;The total antioxidant capacity of the coral homogenate was assessed using a kit from Cell Biolabs (STA-360) according to the manufacturer’s instructions. First, 20 µL of the coral fraction was transferred to each of two wells of a 96-well plate (technical replicates), followed by the addition of 1x reaction buffer (20 µL) from the kit. An initial absorbance reading was collected with a spectrophotometer at 490 nm. Next, 50 µL of 1x copper ion reagent were added to each well, followed by incubation for 5 minutes with gentle shaking. Following shaking, 50 µL of 1x stop solution was added to each well, and a second absorbance reading was collected at 490 nm. Each plate contained a set of standards with known concentrations (0–0.1 mM) of uric acid. For analysis, the initial absorbance readings were subtracted from the final absorbance readings for each standard well, and the resulting values were used to generate a linear standard curve relating the absorbance (x) to the concentration of uric acid (y). The standard curve equation was used with the average change in absorbance across the two technical replicates for each sample to first determine the concentration of uric acid equivalents in the samples, which was then converted to a concentration of copper reducing equivalents (CRE) using the equivalence of 1 mM of uric acid to 2189 μM CREs. Finally, the CRE values were normalized to the amount of protein in each well, for a final value of TAC expressed as µM CRE mg protein-1.&lt;/p&gt; &lt;p&gt;To assess the melanin content of the coral homogenate, a protocol was adapted from (Wall et al., 2021). First, 1.5 mL tubes were filled with approximately 20 mg of the dry coral fraction and the weight recorded. Next, 300 µL of 10 M NaOH was added to each tube, followed by vortexing for 20 seconds. Samples were left overnight at room temperature in the dark, then vortexed again for 10 seconds before being centrifuged at 7000 x g for 5 minutes. Following centrifugation, 100 µL of supernatant was transferred to each of two wells of a 96-well plate (technical replicates), which was then read for absorbance on a spectrophotometer at 490 nm. Each plate contained a set of internal standards with known concentrations (0–0.01 mg mL-1) of synthetic melanin (M8631, Sigma Aldrich) in 10 M NaOH, which were used to generate a linear standard curve relating absorbance (x) to melanin concentration (y). The standard curve equation was then used to calculate the concentration of melanin in each sample, which was standardized to the original weight of the dry coral host tissue used in the assay.&lt;/p&gt; &lt;p&gt;A protocol was adapted from (Wall et al., 2021; Mydlarz and Palmer, 2011; Fuess et al., 2018) to assess the content of prophenoloxidase (PPO) in each coral sample. First, 150 µL of the coral fraction was transferred to each of two wells of a 96-well plate (technical replicates). Next, 23 µL of 0.2 mg mL-1 trypsin was added to each well, and the plate was incubated for 5 minutes at room temperature with shaking. Following incubation, 60 µL of 10 mM L-1,3- dihydroxyphenylalanine (L-DOPA) was added to all wells, and the plate was again incubated at room temperature for 5 minutes with shaking. Next, the plate was read for absorbance on a spectrophotometer at 490 nm every minute for 15 minutes. The absorbance of each well at the start of the 15 minutes was subtracted from the absorbance of the same well at the end, and values were divided by 15 to determine the change in absorbance per minute, which were then averaged over the two technical replicates. Finally, these values were standardized to the amount of protein in each well for a final quantification of PPO activity expressed as the change in absorbance minute-1 mg protein-1&lt;/p&gt; &lt;p&gt;Wax-dipping was used to determine calcium carbonate (CaCO3) bulk density, where the skeleton was cleaned, dried to a constant mass and weighed, sealed with a coat of wax, dry weighed with the wax and then buoyant weighed in DI water at 20°C (Tambutté, et al. 2015; Brown and Mello-Athayde, 2022). The difference between dry weight (with wax) and buoyant weight (divided by the density of the DI water medium of 1 mg cm-3) was calculated to determine the total volume enclosed. The dry skeletal mass (wax free) was then divided by the total volume enclosed to yield bulk density (g cm-3).&lt;/p&gt; &lt;p&gt;Taxonomic Identifiers:&lt;br /&gt; Montipora capitata, LSID&amp;nbsp;(urn:lsid:marinespecies.org:taxname:287697)&lt;br /&gt; Porites compressa, LSID&amp;nbsp;(urn:lsid:marinespecies.org:taxname:207236)&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2102989 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2102989 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2103067 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2103067 completed Katie Barott University of Pennsylvania 215-746-6911 433 South University Avenue Leidy Laboratories 326 Philadelphia PA 19104 USA kbarott@sas.upenn.edu pointOfContact Hollie Putnam University of Rhode Island 808-554-1462 120 Flagg Rd. Kingston RI 02881 USA hputnam@uri.edu pointOfContact Kristen Brown University of Pennsylvania 433 S University Ave Philadelphia PA 19104-4544 USA ktbrown@sas.upenn.edu pointOfContact asNeeded Dataset Version: 1 Unknown Coral_ID Species Bleach Date Period Months Protein Sym_density Chl CaCO3 internal_volume_percent TAC_CRE ug_Chl_a_per_zoox pg_Chl_a_per_zoox Citrate_synthase AFDW Endolithic PPO Melanin_per_tissue Lipid Pmax R PR Guava easyCyte 5HT Diving-PAM (Walz GmbH) OXY-10 (PreSens) optical analyser BioTek PowerWave XS2 theme None, User defined sample identification species scientific name (binomial) sample description date time_elapsed protein concentration density chlorophyll a Calcium Carbonate No BCO-DMO term dry_wgt_globec lipids in biota max photosynthesis photosynthetic rate featureType BCO-DMO Standard Parameters Flow Cytometer Fluorometer PreSens OXY-10 Mini oxygen meter Spectrophotometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance https://osprey.bco-dmo.org/project/868513 RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance <p><em>NSF Award Abstract:</em><br /> Coral bleaching has become increasingly common on reefs worldwide as rising sea surface temperatures associated with climate change disrupt the coral-algal symbiosis. This dramatic heat stress response turns the normally colorful corals bright white, and yet during these heat stress events not all corals undergo bleaching. This project focuses on assessing the effects of bleaching by comparing pairs of corals side-byside on the reef during an ongoing heat wave, where one has bleached and the other has not, despite experiencing the same temperatures. These coral pairs have been monitored throughout three bleaching events in the past five years, providing a unique resource to address whether corals with consistently different bleaching susceptibilities have the capacity to acclimate in response to disturbances through epigenetic changes, or changes in gene expression not due to change in DNA bases. To address this, the project will characterize the impacts of bleaching or not bleaching on coral physiology, gene expression, and epigenetic patterns using coral pairs in their natural habitat during a marine heatwave. This project also provides research support for graduate student trainees, as well as data and materials for the research and training of undergraduate and high school students. This project will recruit underrepresented minority students from URI and UPenn area high schools and university undergraduates for work on computer analysis of images (benthic and colony photographs, brightfield and confocal micrographs) and sequencing data. It will also support the training of an undergraduate student at the University of Hawaiʻi in coral ecology and physiology, and the development of her senior thesis.</p> <p>This project will investigate the effects of repeated heat stress events on the performance of Montipora capitata, a dominant reef builder throughout Hawaiʻi. It utilizes the timely context of paired colonies of M. capitata with bleached vs. unbleached histories that have been monitored through two past bleaching events in Hawaiʻi (2015 and 2019) and the currently ongoing 2020 event. This system allows for the unique opportunity to disentangle the consequences of heat stress versus bleaching on coral performance through time, an essential feature of reef resilience. The contrasting physiological and energetic processes these two phenotypes undergo during a heatwave are likely to result in alterations to the cellular environment within the animal that impacts epigenetic transcriptional regulation. These regulatory and energetic changes, if persistent over time, have the potential to alter coral fitness beyond the duration of the heatwave differentially between corals with contrasting bleaching phenotypes. Specifically, the project will: 1) quantify the effect of the 2020 heatwave on coral physiology during bleaching and recovery, 2) generate a corresponding archive of coral tissues and nucleic acids as a resource for future work characterizing how bleaching phenotype alters energetics and non-genetic inheritance, and 3) characterize how bleaching phenotype alters intra-generational inheritance of epigenetic marks (i.e., DNA methylation) and gene expression, and the duration of these marks and expression patterns following heat stress. This project represents an urgent assessment of an ideal system to test the legacy of coral bleaching phenotype on coral fitness. The results of this project will therefore lay the foundation for intra and cross-generational effects of bleaching vs. heat stress, which is essential for understanding coral resilience to climate change.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Mcap pairs time series largerWorkCitation project eng; USA oceans -157.7954 -157.7954 21.4509 21.4509 2019-10-01 2022-09-30 Coral reefs of Kaneohe Bay, Oahu, Hawaii 0 BCO-DMO catalogue of parameters from Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻi Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/915322.rdf Name: Coral_ID Units: unitless Description: <p>Coral_ID (genotype). numeric identifier</p> http://lod.bco-dmo.org/id/dataset-parameter/915323.rdf Name: Species Units: unitless Description: <p>Genus and species</p> http://lod.bco-dmo.org/id/dataset-parameter/915324.rdf Name: Bleach Units: unitless Description: <p>Bleaching phenotype (&quot;Bleach&quot; or &quot;Non-bleach&quot;)</p> http://lod.bco-dmo.org/id/dataset-parameter/915325.rdf Name: Date Units: unitless Description: <p>Date</p> http://lod.bco-dmo.org/id/dataset-parameter/915326.rdf Name: Period Units: unitless Description: <p>Period (heatwave or recovery)</p> http://lod.bco-dmo.org/id/dataset-parameter/915327.rdf Name: Months Units: unitless Description: <p>Months (post-heat stress). Numeric</p> http://lod.bco-dmo.org/id/dataset-parameter/915328.rdf Name: Protein Units: micrograms per centimeter squared (mg cm-2) Description: <p>Protein</p> http://lod.bco-dmo.org/id/dataset-parameter/915329.rdf Name: Sym_density Units: 10^-6 cells per centimeter squared (10-6 cells cm-2) Description: <p>Endosymbiont density</p> http://lod.bco-dmo.org/id/dataset-parameter/915330.rdf Name: Chl Units: micrograms per centimeter squared (ug cm-2) Description: <p>Chlorophyll a</p> http://lod.bco-dmo.org/id/dataset-parameter/915331.rdf Name: CaCO3 Units: grams ler milliliter (g mL-1) Description: <p>CaCO3 density</p> http://lod.bco-dmo.org/id/dataset-parameter/915332.rdf Name: internal_volume_percent Units: percent (%) Description: <p>Internal volume percent</p> http://lod.bco-dmo.org/id/dataset-parameter/915333.rdf Name: TAC_CRE Units: uM copper reducing equivalents (CRE) mg protein-1 Description: <p>Total antioxidant capacity; copper reducing equivalents (CRE)</p> http://lod.bco-dmo.org/id/dataset-parameter/915334.rdf Name: ug_Chl_a_per_zoox Units: micrograms per cell (ug cell-1) Description: <p>Chlorophyll a per zoox (µg cell-1)</p> http://lod.bco-dmo.org/id/dataset-parameter/915335.rdf Name: pg_Chl_a_per_zoox Units: picograms per cell (ug cell-1) Description: <p>Chlorophyll a per zoox (pg cell-1)</p> http://lod.bco-dmo.org/id/dataset-parameter/915336.rdf Name: Citrate_synthase Units: citrate synthase mg protein-1 Description: <p>Citrate synthase mU mg protein-1</p> http://lod.bco-dmo.org/id/dataset-parameter/915337.rdf Name: AFDW Units: milligrams per centimeter squared (mg cm-2) Description: <p>Ash-free dry weight</p> http://lod.bco-dmo.org/id/dataset-parameter/915338.rdf Name: Endolithic Units: unitless Description: <p>Endolithic(presence or absense of endolithic algae)</p> http://lod.bco-dmo.org/id/dataset-parameter/915339.rdf Name: PPO Units: change in absorbance minute-1 mg protein-1 Description: <p>PPO(prophenoloxidase)</p> http://lod.bco-dmo.org/id/dataset-parameter/915340.rdf Name: Melanin_per_tissue Units: unitless Description: <p>Melanin per tissue (see methodology for details of the melanin concentration calculation in each sample standardized to the original weight of the dry coral host tissue)</p> http://lod.bco-dmo.org/id/dataset-parameter/915341.rdf Name: Lipid Units: grams per cenitmeter squared ( g cm-2) Description: <p>Lipids</p> http://lod.bco-dmo.org/id/dataset-parameter/915342.rdf Name: Pmax Units: micromoles of oxygen per centimeter squared per hour (umol O2 cm2 hr-1) Description: <p>Max photosynthesis (Pmax)</p> http://lod.bco-dmo.org/id/dataset-parameter/915343.rdf Name: R Units: micromoles of oxygen per centimeter squared per hour (umol O2 cm2 hr-1) Description: <p>light-enhanced dark respiration (LEDR)</p> http://lod.bco-dmo.org/id/dataset-parameter/915344.rdf Name: PR Units: unitless Description: <p>PR (photosynthesis to respiration ratio)</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 61468 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c90bb03f-5d55-45ca-909a-662a6ea94a86/content download https://doi.org/10.26008/1912/bco-dmo.904962.1 download onLine dataset &lt;p&gt;Location:&amp;nbsp;&lt;br /&gt; patch reef 13 in Kāne'ohe Bay, O'ahu, Hawai'i (21.4509, -157.7954)&amp;nbsp;&lt;br /&gt; &lt;br /&gt; Physiological analyses:&lt;/p&gt; &lt;p&gt;A total of 276 fragments were collected from colonies of bleaching-resistant and bleaching-susceptible M. capitata and P. compressa (n= 7–10 pairs per phenotype per species) across eight timepoints from October 2019 to September 2022, incorporating the peak of the 2019 heatwave and three years (35 months) of recovery during non-heatwave years (Fig. S1,&amp;nbsp;Brown et al. (2023)). Fragments (4–5 cm in length) were collected by hand using bone cutters, transported to the Hawai’i Institute of Marine Biology (HIMB) in ambient seawater and held in flow-through seawater aquaria for 24–72 hours until measurements of coral performance. For four timepoints (October 2019, October 2021, March 2022, and September 2022), metabolic rates were assessed via changes in oxygen evolution using oxygen optodes (PSt7, PreSens) connected to an optical analyser (OXY-10, PreSens) (Innis et al., 2021). Oxygen optodes were calibrated on each day with a 0% oxygen solution (0.01 g ml−1 NaSO3) and 100% air saturated seawater. Coral fragments were analyzed between 08:00 and 17:00 within 250 ml clear acrylic chambers on top of a magnetic stirrer to allow for continuous mixing. Seawater temperatures were replicated to those experienced on the reef by using ambient seawater and a water jacket to maintain temperatures within the incubation chambers. Temperature and dissolved oxygen concentrations were recorded every 3 seconds at increasing increments of light over a total of 45–50 minutes to determine maximum net photosynthesis (Pmax). Light levels ranged from 112–726 µmol m-2 sec-1 in October 2019 &amp;nbsp;(Innis et al., 2021) (step size ~100 µmol m-2 sec-1; ~10 minutes step-1), and from 387–1800 µmol m-2 sec-1 in 2020–2021 (step size ~300 µmol m-2 sec-1; ~10 minutes step-1). After measurements were completed at the maximum light levels, the lights were turned off (0 µmol m-2 sec-1) to measure light-enhanced dark respiration (LEDR) as defined by (Edmunds and&amp;nbsp;Davies, 1988). Photosynthesis-irradiance curves were fitted using the Platt model to extract Pmax and LEDR (Innis et al., 2021; Platt et al., 1981 ), 3). Upon completion of these in vivo analyses, coral fragments were flash frozen in liquid nitrogen and stored at −80°C until further processing.&lt;/p&gt; &lt;p&gt;Coral tissues containing symbionts were removed from skeletons with a waterpik using 50 mL of 0.1 M phosphate buffered saline (PBS) solution. The resulting holobiont homogenate was centrifuged at 4°C for 5 minutes at 2500 x g to separate the coral host fraction (supernatant) from the intracellular endosymbiont cells (family Symbiodiniaceae; pellet). Symbiodiniaceae were resuspended in PBS and cell abundances were quantified using a Millipore Guava flow-cytometer (Guava easyCyte 5HT) following the methodology of (Innis et al., 2021). Symbiodiniaceae cells were excited with a blue laser (488 nm) and identified by analyzing forward scatter and red autofluorescence in GuavaSoft 3.4 with the same gating for all samples. Symbiont densities were standardized to skeletal surface area (cm2), which was determined using the wax-dipping technique (Holmes, 2008;&amp;nbsp;Stimson and&amp;nbsp;Kinzie, 1991). Endosymbiont photopigments were extracted in 100% acetone for 24 hours and the concentration of chlorophyll-a was determined via absorbance at 630, 663, and 750 nm using the equation in (Jeffrey and Humphrey, 1975) and subtracting the absorbance at 750 nm from both A663 and A630:&amp;nbsp;&lt;/p&gt; &lt;p&gt;Chlorophyll a =(11.43 (A663-A750)) -(0.64 (A630-A750))&lt;/p&gt; &lt;p&gt;Photopigment concentrations were standardized to both skeletal surface area and symbiont densities. Endosymbiont community composition (proportion Durusdinium vs. Cladocopium) of M. capitata colonies was determined by Dilworth et al. 2021 from samples collected in July 2019 using quantitative PCR.&amp;nbsp;&lt;/p&gt; &lt;p&gt;Host tissue biomass was determined as ash-free dry weight (AFDW) from the coral fraction (i.e. symbionts removed as described above). First, 1 mL of the coral fraction was dried at 60°C for 24 hours until a constant weight was achieved. After the dry weight was recorded, the samples were then burned in a muffle furnace at 450°C for 6 hours. The samples were allowed to cool in the furnace before being weighed and the weight of the resulting ash was recorded. The difference between the ash weight and the dry weight was calculated to determine the AFDW of each sample. Host soluble protein content of each sample was determined via the Bradford method. Specifically, 10 μL of the coral fraction was pipetted into each of three wells of a 96-well plate (technical replicates), followed by the addition of 300 μL of Coomassie Plus Bradford reagent (Thermo Fisher Scientific) to each well. The plate was incubated for 10 minutes at room temperature followed by the collection of an absorbance reading at 595 nm on a spectrophotometer (BioTek PowerWave XS2). Each plate contained a set of internal standards with known concentrations of bovine serum albumin (0–2000 μg mL-1), which were used to generate a second-degree polynomial standard curve relating absorbance (x) to protein concentration (y). The standard curve equation was used to calculate the protein concentrations of the samples. The concentration of lipids in each sample was determined via a modified method of (Dunn et al., 2012). First, ~45 mL of the coral fraction was lyophilized to produce dry tissue. A subsample of the lyophilized tissue was weighed (~90 mg), and 2 mL of chloroform-methanol (2:1) was added. The sample was then mixed using a homogenizer at 15000 rpm for 5 seconds, and an additional 2 mL of chloroform-methanol was used to rinse the homogenizer into the sample, and the tubes were then vigorously vortexed before being placed at 4°C for 2 hours in the dark. After incubation, tubes were again vortexed and the chilled homogenates were then passed through 0.22 μm syringe filters into new pre-weighed tubes. An additional 1 mL of chloroform-methanol was passed through the filters into each tube to ensure the full passage of sample material. Next, 1 mL of 0.1 M KCl was added to the samples, which were then vortexed and placed at 4°C for at least 1 hour in the dark until two phases formed. The aqueous (top) phase was discarded, and 5 mL of 50% methanol was added to the organic (bottom) phase. The samples were then placed at 4°C for 1 hour in the dark, followed by removal of the aqueous (top) phase. Washes with 50% methanol were repeated twice more, and the remaining organic phases were dried under a fume hood until the solvent had completely evaporated, at which point the lipid pellet was weighed. Coral host biomass, protein and lipid content were standardized to skeletal surface area.&amp;nbsp;&lt;/p&gt; &lt;p&gt;The total antioxidant capacity of the coral homogenate was assessed using a kit from Cell Biolabs (STA-360) according to the manufacturer’s instructions. First, 20 µL of the coral fraction was transferred to each of two wells of a 96-well plate (technical replicates), followed by the addition of 1x reaction buffer (20 µL) from the kit. An initial absorbance reading was collected with a spectrophotometer at 490 nm. Next, 50 µL of 1x copper ion reagent were added to each well, followed by incubation for 5 minutes with gentle shaking. Following shaking, 50 µL of 1x stop solution was added to each well, and a second absorbance reading was collected at 490 nm. Each plate contained a set of standards with known concentrations (0–0.1 mM) of uric acid. For analysis, the initial absorbance readings were subtracted from the final absorbance readings for each standard well, and the resulting values were used to generate a linear standard curve relating the absorbance (x) to the concentration of uric acid (y). The standard curve equation was used with the average change in absorbance across the two technical replicates for each sample to first determine the concentration of uric acid equivalents in the samples, which was then converted to a concentration of copper reducing equivalents (CRE) using the equivalence of 1 mM of uric acid to 2189 μM CREs. Finally, the CRE values were normalized to the amount of protein in each well, for a final value of TAC expressed as µM CRE mg protein-1.&lt;/p&gt; &lt;p&gt;To assess the melanin content of the coral homogenate, a protocol was adapted from (Wall et al., 2021). First, 1.5 mL tubes were filled with approximately 20 mg of the dry coral fraction and the weight recorded. Next, 300 µL of 10 M NaOH was added to each tube, followed by vortexing for 20 seconds. Samples were left overnight at room temperature in the dark, then vortexed again for 10 seconds before being centrifuged at 7000 x g for 5 minutes. Following centrifugation, 100 µL of supernatant was transferred to each of two wells of a 96-well plate (technical replicates), which was then read for absorbance on a spectrophotometer at 490 nm. Each plate contained a set of internal standards with known concentrations (0–0.01 mg mL-1) of synthetic melanin (M8631, Sigma Aldrich) in 10 M NaOH, which were used to generate a linear standard curve relating absorbance (x) to melanin concentration (y). The standard curve equation was then used to calculate the concentration of melanin in each sample, which was standardized to the original weight of the dry coral host tissue used in the assay.&lt;/p&gt; &lt;p&gt;A protocol was adapted from (Wall et al., 2021; Mydlarz and Palmer, 2011; Fuess et al., 2018) to assess the content of prophenoloxidase (PPO) in each coral sample. First, 150 µL of the coral fraction was transferred to each of two wells of a 96-well plate (technical replicates). Next, 23 µL of 0.2 mg mL-1 trypsin was added to each well, and the plate was incubated for 5 minutes at room temperature with shaking. Following incubation, 60 µL of 10 mM L-1,3- dihydroxyphenylalanine (L-DOPA) was added to all wells, and the plate was again incubated at room temperature for 5 minutes with shaking. Next, the plate was read for absorbance on a spectrophotometer at 490 nm every minute for 15 minutes. The absorbance of each well at the start of the 15 minutes was subtracted from the absorbance of the same well at the end, and values were divided by 15 to determine the change in absorbance per minute, which were then averaged over the two technical replicates. Finally, these values were standardized to the amount of protein in each well for a final quantification of PPO activity expressed as the change in absorbance minute-1 mg protein-1&lt;/p&gt; &lt;p&gt;Wax-dipping was used to determine calcium carbonate (CaCO3) bulk density, where the skeleton was cleaned, dried to a constant mass and weighed, sealed with a coat of wax, dry weighed with the wax and then buoyant weighed in DI water at 20°C (Tambutté, et al. 2015; Brown and Mello-Athayde, 2022). The difference between dry weight (with wax) and buoyant weight (divided by the density of the DI water medium of 1 mg cm-3) was calculated to determine the total volume enclosed. The dry skeletal mass (wax free) was then divided by the total volume enclosed to yield bulk density (g cm-3).&lt;/p&gt; &lt;p&gt;Taxonomic Identifiers:&lt;br /&gt; Montipora capitata, LSID&amp;nbsp;(urn:lsid:marinespecies.org:taxname:287697)&lt;br /&gt; Porites compressa, LSID&amp;nbsp;(urn:lsid:marinespecies.org:taxname:207236)&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;See results publication&amp;nbsp;Brown, et al. (2023) for&amp;nbsp;more detailed information on analysis and results.&amp;nbsp;&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) * table within file &quot;Phys_data_for_BCO-DMO.csv&quot; was imported into the BCO-DMO data system. ** Missing data values are displayed differently based on the file format you download.  They are blank in csv files, &quot;NaN&quot; in MatLab files, etc. * Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number] * Date converted to ISO 8601 format Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Guava easyCyte 5HT Guava easyCyte 5HT PI Supplied Instrument Name: Guava easyCyte 5HT PI Supplied Instrument Description:endosymbiont densities: Millipore Guava flow-cytometer (Guava easyCyte 5HT) Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Diving-PAM (Walz GmbH) Diving-PAM (Walz GmbH) PI Supplied Instrument Name: Diving-PAM (Walz GmbH) PI Supplied Instrument Description:photochemical yield (Fv/Fm): Diving-PAM (Walz GmbH) Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/ OXY-10 (PreSens) optical analyser OXY-10 (PreSens) optical analyser PI Supplied Instrument Name: OXY-10 (PreSens) optical analyser PI Supplied Instrument Description:net photosynthesis and light-enhanced dark respiration: oxygen optodes connected to an OXY-10 (PreSens) optical analyser Instrument Name: PreSens OXY-10 Mini oxygen meter Instrument Short Name: Instrument Description: The OXY-10 mini is a precise multi-channel oxygen meter for up to 10 'in-house' sensors, simultaneously controlling and reading them. The meter is used with oxygen sensors based on a 2mm optical fibre. The meter is compatible with sensors that are type PSt3 which has a detection limit 15 ppb, 0 - 100% oxygen. BioTek PowerWave XS2 BioTek PowerWave XS2 PI Supplied Instrument Name: BioTek PowerWave XS2 PI Supplied Instrument Description:host-soluble protein concentration, chlorophyll a concentrations, total antioxidant capacity, melanin content, prophenoloxidase content, citrate synthase content: spectrophotometer (BioTek PowerWave XS2) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/