http://lod.bco-dmo.org/id/dataset/905077
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-07-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Ultrahigh resolution Mass Spectrometry data from a depth profile collected at 200 m depth intervals at the Bermuda Atlantic Time Series Station (BATS) in August 2019 and at the Hawaii Ocean Time Series station (HOTS) Aloha in July 2021
2023-09-18
publication
2023-09-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-09-18
publication
https://doi.org/10.26008/1912/bco-dmo.905077.1
Michael Gonsior
University of Maryland Center for Environmental Science
principalInvestigator
Feng Chen
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gonsior, M., Chen, F. (2023) Ultrahigh resolution Mass Spectrometry data from a depth profile collected at 200 m depth intervals at the Bermuda Atlantic Time Series Station (BATS) in August 2019 and at the Hawaii Ocean Time Series station (HOTS) Aloha in July 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.905077.1 [access date]
Ultrahigh Resolution Mass Spectrometry of a Depth Profile Dataset Description: Methods and Sampling: <p>Water samples were collected using the ship's CTD profiler and 12 L Nisking bottles. 10 L water samples from each depth were then transferred into 5 gallon polycarbonate water bottles and extractrated. 10 L seawater samples were collected at 200 m depth intervals and then solid-phase extracted suing Agilent Bond Elut PPL cartridges (1 g, 6 ml). The organic matter was then eluted with 10 mL methanol. The methanolic extract was diluted 1:20 and directly infused into a 12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer. 500 scans were averaged and mass lists were calibrated at a mass accuracy of greater than 0.2 ppm.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829888 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829888
completed
Michael Gonsior
University of Maryland Center for Environmental Science
410-326-7245
146 Williams Street
Solomons
MD
20688-3204
USA
gonsior.michael@gmail.com
pointOfContact
Feng Chen
University of Maryland Center for Environmental Science
410-234-8866
chenf@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station_Location
Latitude
Longitude
Date
Replicate
Depth
Mass
Intensity
12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer
theme
None, User defined
station
latitude
longitude
date
replicate
depth
mass-to-charge ratio (m/z)
Intensity (relative ion abundance)
featureType
BCO-DMO Standard Parameters
Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
instrument
BCO-DMO Standard Instruments
AE1920
KM2110
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
https://osprey.bco-dmo.org/project/806071
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
<p><em>NSF Award Abstract:</em><br />
This study focuses on the sources and composition of colored dissolved organic matter (CDOM) in the ocean. CDOM is a part of water that absorbs sunlight. This material is important because it filters out harmful ultraviolet radiation. Scientists use it to track the movement of carbon and other important biological and chemical processes in the ocean. Organisms such as algae living in the open ocean have been shown to be sources of CDOM, but the chemical composition of these algal natural products remains to be discovered. Recent results from studying common algae show that viruses may break down algal cells and release material that looks like CDOM. This study will use new tools to find out if viruses and algae are creating this material and study its chemical makeup. This project will support two graduate students and provide summer internships for undergraduates through the NSF Research Experiences for Undergraduates (REU) program. The investigators will participate in a range of education and outreach activities.</p>
<p>The sources and structural nature of marine CDOM within the oceans remain unclear and continue to be a subject of debate. Marine in situ sources of CDOM have been suggested and some have been confirmed, but thus far none could explain the ubiquitous appearance of the so called "humic-like" CDOM component. Unique features of this component include its unusual exponential behavior in ultraviolet-visible (UV-Vis) absorbance with the absorbance extending well above 400 nm, and the large Stoke's shift in fluorescence spectroscopy. Picocyanobacteria are ubiquitous in the World's Oceans and make up 50 % of the autotrophic marine primary production. Preliminary results showed that the picocyanobacteria Synechococcus and Prochlorococcus release CDOM that matched the "humic-like" appearance of globally observed marine CDOM after virus-induced lysis. The main focus of this study is the characterization of the optical properties and molecular composition of viral-lysed DOM (VDOM) from different strains of Synechococcus and Prochlorococcus and additionally Trichodesmium which was shown in a previous study to also release CDOM. Associations between the chemical characterization information and metagenomics and transcriptomics data will be investigated for picocyanobacteria in the Pacific and Atlantic Oceans. This study includes long-term incubation experiments to determine the persistence of picocyanobacteria-derived CDOM as well as changes in microbial communities and processes (gene expression) that are related to the degradation of VDOM during the incubation period.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Picocyanobacteria CDOM
largerWorkCitation
project
eng; USA
oceans
-158
-64.166667
22.75
31.833333
2019-08-09
2021-07-10
Bermuda Atlantic Time Series Station (BATS) and station Aloha, Hawaii
0
BCO-DMO catalogue of parameters from Ultrahigh resolution Mass Spectrometry data from a depth profile collected at 200 m depth intervals at the Bermuda Atlantic Time Series Station (BATS) in August 2019 and at the Hawaii Ocean Time Series station (HOTS) Aloha in July 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/908450.rdf
Name: Station_Location
Units: unitless
Description: <p>Sampling station (HOT or BATS)</p>
http://lod.bco-dmo.org/id/dataset-parameter/908451.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude sampling location, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/908452.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude sampling location, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/908453.rdf
Name: Date
Units: unitless
Description: <p>Sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/908454.rdf
Name: Replicate
Units: unitless
Description: <p>Replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/908455.rdf
Name: Depth
Units: meters (m)
Description: <p>Sampling Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/908456.rdf
Name: Mass
Units: unitless
Description: <p>Mass to charge ratio (m/z)</p>
http://lod.bco-dmo.org/id/dataset-parameter/908457.rdf
Name: Intensity
Units: unitless
Description: <p>Ion intensity (arbitrary unit)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
86620988
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/bff0b2be-a0a9-4b3e-8e61-3069223b7d38/content
download
https://doi.org/10.26008/1912/bco-dmo.905077.1
download
onLine
dataset
<p>Water samples were collected using the ship's CTD profiler and 12 L Nisking bottles. 10 L water samples from each depth were then transferred into 5 gallon polycarbonate water bottles and extractrated. 10 L seawater samples were collected at 200 m depth intervals and then solid-phase extracted suing Agilent Bond Elut PPL cartridges (1 g, 6 ml). The organic matter was then eluted with 10 mL methanol. The methanolic extract was diluted 1:20 and directly infused into a 12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer. 500 scans were averaged and mass lists were calibrated at a mass accuracy of greater than 0.2 ppm.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data were calibrated using known DOM signatures and a matrix was generated aligning all spectra. See Timko <em>et al., </em>2015 Reference for the calibration of FT-ICR MS data.</p>
<p>BATS depth 120m = deep chlorophyll maximum (DCM)<br />
BATS depth 820m = oxygen minimum zone (OMZ)</p>
Specified by the Principal Investigator(s)
* unpivot data: transform data from multiple intensity columns with location, depth and replicate in the header and changed it to one intensity column and added columns to capture the location, depth and replicate.
* added sampling date, latitude and longitude
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer
12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer
PI Supplied Instrument Name: 12 Tesla Bruker Solarix Fourier transform ion cyclotron resonance mass spectrometer Instrument Name: Fourier Transform Ion Cyclotron Resonance Mass Spectrometer Instrument Short Name:FTICR MS Instrument Description: In Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, the mass-to-charge ratio (m/z) of an ion is experimentally determined by measuring the frequency at which the ion processes in a magnetic field. These frequencies, which are typically in the 100 KHz to MHz regime, can be measured with modern electronics making it possible to determine the mass of an ion to within +/- 0.000005 amu or 5 ppm.
Cruise: AE1920
AE1920
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1920
Michael Gonsior
University of Maryland Center for Environmental Science
Cruise: KM2110
KM2110
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2110
Michael Gonsior
University of Maryland Center for Environmental Science
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel