http://lod.bco-dmo.org/id/dataset/906617 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-08-22 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia 2023-09-27 publication 2023-09-27 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-10-02 publication https://doi.org/10.26008/1912/bco-dmo.906617.1 Adrienne M.S. Correa Rice University principalInvestigator Rebecca Vega Thurber Oregon State University principalInvestigator Andrew Thurber Oregon State University principalInvestigator Lauren I. Howe-Kerr Rice University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Correa, A. M., Vega Thurber, R., Thurber, A., Howe-Kerr, L. I. (2023) A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral &lt;i&gt;Porites c.f. lobata&lt;/i&gt; at three reef zones around Moorea, French Polynesia. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.906617.1 [access date] dinoRNAV dynamics in the reef-building coral Porites lobata Dataset Description: &lt;p&gt;This dataset is comprised of three components:&lt;/p&gt; &lt;p&gt;(1) &lt;strong&gt;temperature data measured at each reef site&lt;/strong&gt; - available in the csv data file &quot;906617_v1_temperature_moorea_reefs.csv&quot;&lt;/p&gt; &lt;p&gt;(2) &lt;strong&gt;images of Porites lobata colonies taken at each site&lt;/strong&gt; - available in the PDF &quot;POR colony health images.pdf&quot;&lt;/p&gt; &lt;p&gt;(3) &lt;strong&gt;dinoRNAV mcp gene amplicon libraries and Symbiodiniaceae LSU gene amplicon libraries&lt;/strong&gt; - available in the National Center for Biotechnology Information Sequence Read Archive under accession numbers &lt;a href=&quot;https://www.ncbi.nlm.nih.gov/bioproject/PRJNA928208&quot; target=&quot;_blank&quot;&gt;PRJNA928208&lt;/a&gt; and &lt;a href=&quot;https://www.ncbi.nlm.nih.gov/bioproject/PRJNA930706&quot; target=&quot;_blank&quot;&gt;PRJNA930706&lt;/a&gt;, respectively. Parameters used to analyze the viral data are described in Zenodo at &lt;a href=&quot;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&quot; target=&quot;_blank&quot;&gt;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&lt;/a&gt; (doi: &lt;a href=&quot;http://dx.doi.org/10.5281/zenodo.7552892&quot; target=&quot;_blank&quot;&gt;10.5281/zenodo.7552892&lt;/a&gt;)&lt;/p&gt; &lt;p&gt;Results of this study have been published in Howe-Kerr et al., 2023. (doi: &lt;a href=&quot;http://dx.doi.org/10.1038/s43705-023-00227-7&quot; target=&quot;_blank&quot;&gt;10.1038/s43705-023-00227-7&lt;/a&gt;).&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;strong&gt;Sample collection for 'omics analyses (both Symbiodiniaceae LSU and dinoRNAV MCP):&lt;/strong&gt;&lt;br /&gt; Fifty-four colonies of &lt;em&gt;Porites lobata&lt;/em&gt; were tagged on the north shore of Moorea, French Polynesia, spanning nine sites that encompassed three reef zones (fringing, back, and forereef; n = 6 colonies/site, n = 3 sites/reef zone). Each tagged colony was sampled in August 2018 (dry season), March 2019 (wet season), August 2019 (dry), and October 2020 (dry). Samples could not be collected in March 2020 due to the COVID-19 pandemic. Tissue samples were collected for amplicon sequencing; for each &lt;em&gt;P. lobata&lt;/em&gt; colony, ~3 to 6 small fragments (1 square centimeter (cm²)) of skeleton/tissue were sampled across the colony surface using bone cutters, placed in a sterile Whirl-Pak® (Nasco), and then preserved in DNA/RNA shield (ZymoResearch, Irvine, California, USA). Samples in the DNA/RNA shield were kept on ice until returning to shore, at which point they were vortexed for 25 minutes at full speed with 5 ceramic beads and 1.35 grams (g) garnet matrix (MP Biomedicals) and then frozen at -40° Celsius (C). These samples were wrapped in aluminum foil and stored at -40°C until further processing. DNA and RNA were extracted from the coral samples, which included mucus, tissue, and skeleton, using enzyme digestions and a ZymoBIOMICs DNA/RNA Kit (ZymoResearch, Irvine, California, USA, following Grupstra et al., 2022).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Symbiodiniaceae LSU:&lt;/strong&gt;&lt;br /&gt; To identify dominant Symbiodiniaceae lineages, the D1-D2 region of the 28S large subunit (LSU) nuclear ribosomal RNA gene was amplified from coral holobiont DNA. At the Oregon State University Center for Qualitative Life Sciences (CQLS), PCR reactions were conducted using the primers LSU1F_illu and LSU1R_illu with attached Miseq Adapters; cycles were as follows: 95°C for 3 minutes, and then 15 cycles of 95°C for 30 seconds, 56°C for 30 seconds, and 72°C for 30 seconds, and finally, 72°C for 4 minutes. PCR clean-up was completed using Agencourt AMPure XP Magnetic Beads. PCR reactions to incorporate Illumina indexing primers were conducted as follows: 95°C for 3 minutes, and then 20 cycles of 95°C for 30 seconds, 56°C for 30 seconds, and 72°C for 30 seconds, and finally 72°C for 4 minutes. The resulting PCR product was purified with Agencourt AMPure XP Magnetic Beads, quantified via qPCR using the KAPA library quantification kit (Roche Sequencing Solutions, Pleasanton, CA), pooled in equal molar amounts, and sequenced on the Illumina MiSeq platform with PE300 chemistry.&lt;/p&gt; &lt;p&gt;Amplicons of the LSU gene were processed in RStudio (version 1.1.456) through the DADA2 pipeline (version 1.11.0), which generates unique sequences at single-nucleotide resolution (amplicon sequence variants or ‘ASVs’) (Grupstra et al., 2022). Forward and reverse paired reads were truncated at 210 and 160 base pairs, respectively, based on quality plots. Reads with a total expected error of one or more, or contained Ns, were discarded. ASVs were inferred from unique reads, paired reads were merged, ASVs that did not match a target length of 286 ± 5 were removed, and chimeras were detected and removed. This DADA2 curated table of ASVs were then further collapsed using the LULU package (Frøslev et al., 2017), which merges potentially erroneous ASVs based on sequence similarity and co-occurrence patterns (default parameters of 84% similarity, 90% co-occurrence used here; see &lt;a href=&quot;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&quot; target=&quot;_blank&quot;&gt;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&lt;/a&gt;). Taxonomy was assigned to curated ASVs based on BLAST (blastn) searches to NCBI’s nr/nt database, and ASVs that had best matches to non-Symbiodiniaceae were discarded.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;dinoRNAV MCP:&lt;/strong&gt;&lt;br /&gt; The dinoRNAV major capsid protein (mcp) gene was amplified from cDNA (generated from coral holobiont RNA), using a nested PCR protocol with primers from Montalvo-Proaño et al. (2017). Cleaned and normalized libraries were sequenced on the Illumina MiSeq platform using PE300 v3 chemistry at the Oregon State University Center for Qualitative Life Sciences (CQLS). Sequencing and bioinformatic analyses were conducted following Grupstra et al., (2022) using the program vAMPirus (v1.0.1, Veglia et al. 2021). Briefly, after adapter removal, quality filtering, primer removal, read merging, and length filtering, amplicon sequence variants (ASVs) were generated and chimeras removed using VSEARCH with the UNOISE3 algorithm (Rognes et al., 2016; Edgar et al., 2016). Parameters and program information for each of these steps can be found in the vAMPirus config file included as a Supplementary File (&quot;HoweKerr_etal_2023_vAMPirus.config&quot;) in Howe-Kerr et al. (2023) and all non-read files used to run the analyses and generate the results can be found at the vAMPirus Zenodo (&lt;a href=&quot;https://doi.org/10.5281/zenodo.7552892&quot; target=&quot;_blank&quot;&gt;https://doi.org/10.5281/zenodo.7552892&lt;/a&gt;). To collapse some of the diversity associated with the high mutation rate of ssRNA viruses, ASVs were then translated and aligned into unique amino acid types ('aminotypes') using VirtualRibosome (v2.0, Wernersson et al., 2006) and CD-HIT (v.4.8.1, Fu et al., 2012).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Temperature:&lt;/strong&gt;&lt;br /&gt; Water temperatures were measured every two hours year-round at each site for the duration of the study using a HOBO® temperature logger.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Colony images:&lt;/strong&gt;&lt;br /&gt; Photographs of each colony were taken at each sampling point and used in visual assessments to determine if a colony remained apparently healthy or experienced partial mortality over the course of the study; a third category ('ambiguous') was used to describe colonies for which health trajectory could not be determined based on the images available. Colonies were considered to have experienced partial mortality if there was a clear loss of live tissue surface area between August 2018 and October 2020; colonies were considered to have remained apparently healthy if there was an increase in live tissue in images taken over the course of the assessed period or if there was no clear change in live tissue surface area. Final health determination was based on assessments by three separate observers, who assessed the images separately and blindly without knowing what reef zone or site a set of images was collected from. Differences in coral health among reef types and sites were assessed with Chi-squared tests.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Known issues or problems:&lt;/strong&gt;&lt;br /&gt; Due to logistical issues, HOBO® temperature logger data are not available for October 2020.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635798 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635798 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635913 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635913 Funding provided by Gulf Research Program of the National Academies of Sciences, Engineering, and Medicine (GRP) Award Number: Early-Career Research Fellowship #2000009651 completed Adrienne M.S. Correa Rice University amsc@berkeley.edu pointOfContact Rebecca Vega Thurber Oregon State University rvegathurber@gmail.com pointOfContact Andrew Thurber Oregon State University 541-737-4500 athurber@ucsb.edu pointOfContact Lauren I. Howe-Kerr Rice University 1-719-252-4909 6100 Main Street MS-140 Houston TX 77005 USA lih2@rice.edu pointOfContact asNeeded Dataset Version: 1 Unknown site_number reef_zone Latitude Longitude temperature_reading_number ISO_DateTime_Local ISO_DateTime_UTC temp_celsius Illumina MiSeq GoPro and Olympus Tough camera HOBO® temperature logger (Onset brands, # UA-002-64) theme None, User defined site site description latitude longitude sample identification ISO_DateTime_Local ISO_DateTime_UTC water temperature at measurement depth featureType BCO-DMO Standard Parameters Automated DNA Sequencer Camera Temperature Logger instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling https://osprey.bco-dmo.org/project/713328 Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling <p>Ecologically and economically, coral reefs are among the most valuable ecosystems on Earth. These habitats are estimated to harbor up to nine million species, contribute ~30 billion US dollars annually to the global economy, and are tropical epicenters of biogeochemical cycling. Global (climate change) and local (nutrient pollution and overfishing) stressors are drivers of coral reef decline that can disrupt the symbiotic associations among corals and resident microbial communities, including dinoflagellate algae, bacteria, and viruses. Viruses interact with all living cellular organisms, are abundant in oceans, and integral to marine ecosystem functioning. This project will be the first to quantify the variability of viral infection in corals across different reef habitats and across time. This will increase our understanding of the total diversity of coral viruses and illuminate the full suite of factors that trigger viral outbreaks on reefs. At the same time the project will evaluate how carbon and nitrogen cycling are altered on coral reefs as a result of global and local stressors that trigger viral infection. This project will ultimately broaden our understanding of the impacts of viruses on reefs beyond their role as putative disease agents. Results of the project will be communicated broadly in scientific arenas, in K-12, undergraduate, and graduate education and training programs, and to the general public through video and multimedia productions, as well as outreach events. 2-D Reef Replicas from our field sites across Moorea will be constructed, allowing children and adults in the US and French Polynesia to 'become' marine scientists and use quadrats, transect tapes, and identification guides to quantify metrics of reef change. Three graduate students will be involved in all aspects of the research and an effort will be made to recruit and support minority students. All datasets will be made freely available to the public and newly developed methods from this project will serve as an important set of springboard tools and baselines for future lines of inquiry into the processes that influence reef health.</p> <p>Coral reefs, found in nutrient-poor shallow waters, are biodiversity and productivity hotspots that provide substantial ecological and societal benefits. Corals energetically subsidize these oligotrophic ecosystems by releasing significant amounts of mucus (an organic carbon and nitrogen-rich matrix) into the surrounding seawater. Viral production in reef waters can be a significant portion of total reef carbon cycling, accounting for ~10% of gross benthic carbon fixation in reef ecosystems. Viruses are also ~10 times more abundant on coral surfaces than in the water column meaning that viral infection experienced by corals during stress likely results is an increase in carbon and perhaps nitrogen flux to the water column. Thus phages and eukaryotic viruses may be responsible for shifting reef health and function directly via coral and symbiont infection and by altering biogeochemical cycling in host colonies and the adjacent reef system. The main goal of this project is to experimentally interrogate and then model the links among viral infections, declines in coral and reef health, and associated shifts in biogeochemical cycling in reef ecosystems. Lab and field experiments will be conducted at the Moorea Coral Reef LTER to characterize the spatiotemporal dynamics of viruses within two dominant reef-building coral species that differ in their susceptibility to abiotic stress. A novel viral infection and induction approach will be coupled with stable isotopic pulse-chase experiments to quantify and track carbon and nitrogen flux out of coral holobionts (host and microbial symbionts) and into dissolved and particulate pools. In these experiments, virus, bacteria, and symbiont abundance, diversity, and function will be measured simultaneously with the health and activity of the host. Pulse-chase techniques, as well as flux- and niche-based modeling, will result in a holistic understanding of how corals and associated viruses impact reef energy budgets and the ramifications of carbon and nitrogen flux for reef communities. Ultimately, this project will quantify and describe an integrated mechanism by which environmental stressors alter viral, microbial, and coral diversity and, consequently, ecosystem function.</p> Moorea Virus Project largerWorkCitation project eng; USA oceans -149.853 -149.8 -17.5084 -17.4721 2017-09-12 2020-10-28 Moorea, French Polynesia, Pacific 17 S 150 W 0 BCO-DMO catalogue of parameters from A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/906631.rdf Name: site_number Units: unitless Description: <p>Site ID number, refers to the site location within reef zone</p> http://lod.bco-dmo.org/id/dataset-parameter/906632.rdf Name: reef_zone Units: unitless Description: <p>Reef type: &quot;fringe&quot; = Fringing reef (closest to shore) ;&quot;back&quot; = Back reef (lagoon); &quot;fore&quot; = Forereef (furthest from shore)</p> http://lod.bco-dmo.org/id/dataset-parameter/906633.rdf Name: Latitude Units: decimal degrees Description: <p>Latitude of the reef site; negative values = South</p> http://lod.bco-dmo.org/id/dataset-parameter/906634.rdf Name: Longitude Units: decimal degrees Description: <p>Longitude of the reef site; negative values = West</p> http://lod.bco-dmo.org/id/dataset-parameter/906635.rdf Name: temperature_reading_number Units: unitless Description: <p>ID number that indicates a unique reading; readings occurred every two hours</p> http://lod.bco-dmo.org/id/dataset-parameter/906636.rdf Name: ISO_DateTime_Local Units: unitless Description: <p>Date and time of the temperature reading in the local time zone (GMT-10); in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/906637.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Date and time of the temperature reading in UTC; in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/906638.rdf Name: temp_celsius Units: degrees Celsius Description: <p>Temperature reading</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 7934407 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/48b239ed-8a1e-4554-8269-9927db802ff4/content download https://doi.org/10.26008/1912/bco-dmo.906617.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Sample collection for 'omics analyses (both Symbiodiniaceae LSU and dinoRNAV MCP):&lt;/strong&gt;&lt;br /&gt; Fifty-four colonies of &lt;em&gt;Porites lobata&lt;/em&gt; were tagged on the north shore of Moorea, French Polynesia, spanning nine sites that encompassed three reef zones (fringing, back, and forereef; n = 6 colonies/site, n = 3 sites/reef zone). Each tagged colony was sampled in August 2018 (dry season), March 2019 (wet season), August 2019 (dry), and October 2020 (dry). Samples could not be collected in March 2020 due to the COVID-19 pandemic. Tissue samples were collected for amplicon sequencing; for each &lt;em&gt;P. lobata&lt;/em&gt; colony, ~3 to 6 small fragments (1 square centimeter (cm²)) of skeleton/tissue were sampled across the colony surface using bone cutters, placed in a sterile Whirl-Pak® (Nasco), and then preserved in DNA/RNA shield (ZymoResearch, Irvine, California, USA). Samples in the DNA/RNA shield were kept on ice until returning to shore, at which point they were vortexed for 25 minutes at full speed with 5 ceramic beads and 1.35 grams (g) garnet matrix (MP Biomedicals) and then frozen at -40° Celsius (C). These samples were wrapped in aluminum foil and stored at -40°C until further processing. DNA and RNA were extracted from the coral samples, which included mucus, tissue, and skeleton, using enzyme digestions and a ZymoBIOMICs DNA/RNA Kit (ZymoResearch, Irvine, California, USA, following Grupstra et al., 2022).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Symbiodiniaceae LSU:&lt;/strong&gt;&lt;br /&gt; To identify dominant Symbiodiniaceae lineages, the D1-D2 region of the 28S large subunit (LSU) nuclear ribosomal RNA gene was amplified from coral holobiont DNA. At the Oregon State University Center for Qualitative Life Sciences (CQLS), PCR reactions were conducted using the primers LSU1F_illu and LSU1R_illu with attached Miseq Adapters; cycles were as follows: 95°C for 3 minutes, and then 15 cycles of 95°C for 30 seconds, 56°C for 30 seconds, and 72°C for 30 seconds, and finally, 72°C for 4 minutes. PCR clean-up was completed using Agencourt AMPure XP Magnetic Beads. PCR reactions to incorporate Illumina indexing primers were conducted as follows: 95°C for 3 minutes, and then 20 cycles of 95°C for 30 seconds, 56°C for 30 seconds, and 72°C for 30 seconds, and finally 72°C for 4 minutes. The resulting PCR product was purified with Agencourt AMPure XP Magnetic Beads, quantified via qPCR using the KAPA library quantification kit (Roche Sequencing Solutions, Pleasanton, CA), pooled in equal molar amounts, and sequenced on the Illumina MiSeq platform with PE300 chemistry.&lt;/p&gt; &lt;p&gt;Amplicons of the LSU gene were processed in RStudio (version 1.1.456) through the DADA2 pipeline (version 1.11.0), which generates unique sequences at single-nucleotide resolution (amplicon sequence variants or ‘ASVs’) (Grupstra et al., 2022). Forward and reverse paired reads were truncated at 210 and 160 base pairs, respectively, based on quality plots. Reads with a total expected error of one or more, or contained Ns, were discarded. ASVs were inferred from unique reads, paired reads were merged, ASVs that did not match a target length of 286 ± 5 were removed, and chimeras were detected and removed. This DADA2 curated table of ASVs were then further collapsed using the LULU package (Frøslev et al., 2017), which merges potentially erroneous ASVs based on sequence similarity and co-occurrence patterns (default parameters of 84% similarity, 90% co-occurrence used here; see &lt;a href=&quot;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&quot; target=&quot;_blank&quot;&gt;https://zenodo.org/record/7552892#.Y_PAa-zMK3I&lt;/a&gt;). Taxonomy was assigned to curated ASVs based on BLAST (blastn) searches to NCBI’s nr/nt database, and ASVs that had best matches to non-Symbiodiniaceae were discarded.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;dinoRNAV MCP:&lt;/strong&gt;&lt;br /&gt; The dinoRNAV major capsid protein (mcp) gene was amplified from cDNA (generated from coral holobiont RNA), using a nested PCR protocol with primers from Montalvo-Proaño et al. (2017). Cleaned and normalized libraries were sequenced on the Illumina MiSeq platform using PE300 v3 chemistry at the Oregon State University Center for Qualitative Life Sciences (CQLS). Sequencing and bioinformatic analyses were conducted following Grupstra et al., (2022) using the program vAMPirus (v1.0.1, Veglia et al. 2021). Briefly, after adapter removal, quality filtering, primer removal, read merging, and length filtering, amplicon sequence variants (ASVs) were generated and chimeras removed using VSEARCH with the UNOISE3 algorithm (Rognes et al., 2016; Edgar et al., 2016). Parameters and program information for each of these steps can be found in the vAMPirus config file included as a Supplementary File (&quot;HoweKerr_etal_2023_vAMPirus.config&quot;) in Howe-Kerr et al. (2023) and all non-read files used to run the analyses and generate the results can be found at the vAMPirus Zenodo (&lt;a href=&quot;https://doi.org/10.5281/zenodo.7552892&quot; target=&quot;_blank&quot;&gt;https://doi.org/10.5281/zenodo.7552892&lt;/a&gt;). To collapse some of the diversity associated with the high mutation rate of ssRNA viruses, ASVs were then translated and aligned into unique amino acid types ('aminotypes') using VirtualRibosome (v2.0, Wernersson et al., 2006) and CD-HIT (v.4.8.1, Fu et al., 2012).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Temperature:&lt;/strong&gt;&lt;br /&gt; Water temperatures were measured every two hours year-round at each site for the duration of the study using a HOBO® temperature logger.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Colony images:&lt;/strong&gt;&lt;br /&gt; Photographs of each colony were taken at each sampling point and used in visual assessments to determine if a colony remained apparently healthy or experienced partial mortality over the course of the study; a third category ('ambiguous') was used to describe colonies for which health trajectory could not be determined based on the images available. Colonies were considered to have experienced partial mortality if there was a clear loss of live tissue surface area between August 2018 and October 2020; colonies were considered to have remained apparently healthy if there was an increase in live tissue in images taken over the course of the assessed period or if there was no clear change in live tissue surface area. Final health determination was based on assessments by three separate observers, who assessed the images separately and blindly without knowing what reef zone or site a set of images was collected from. Differences in coral health among reef types and sites were assessed with Chi-squared tests.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Known issues or problems:&lt;/strong&gt;&lt;br /&gt; Due to logistical issues, HOBO® temperature logger data are not available for October 2020.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Temperature data processing:&lt;/strong&gt;&lt;br /&gt; Raw temperature values at the start and end of each HOBO® logger launch were removed (since these were temperature recordings that occurred before or after deployment to the reef).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Colony image processing:&lt;/strong&gt;&lt;br /&gt; Colony images are grouped by Reef Type, Site, and Colony ID; all images of the same colony over time are depicted on the same page.&lt;/p&gt; Specified by the Principal Investigator(s) - Created a table out of the site locations (lats/lons) provided in the parameters section of the metadata; saved as a CSV file named &quot;site_coordinates.csv&quot;. - Imported original temperature file &quot;Hobodata_temperature_filt.xlsx&quot; into the BCO-DMO system, along with &quot;site_coordinates.csv&quot;. - Joined latitude and longitude columns from the site coordinates file to the temperature data file by matching on site_number and reef_zone. - Converted the date/time column to ISO 8601 format in the local time zone (GMT-10). - Created a date/time column in ISO 8601 format in the UTC time zone. - Removed the &quot;row_number&quot; column (unnecessary for data reuse). - Saved the final temperature file as &quot;906617_v1_temperature_moorea_reefs.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Illumina MiSeq Illumina MiSeq PI Supplied Instrument Name: Illumina MiSeq Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences. GoPro and Olympus Tough camera GoPro and Olympus Tough camera PI Supplied Instrument Name: GoPro and Olympus Tough camera Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/ HOBO® temperature logger (Onset brands, # UA-002-64) HOBO® temperature logger (Onset brands, # UA-002-64) PI Supplied Instrument Name: HOBO® temperature logger (Onset brands, # UA-002-64) Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.