http://lod.bco-dmo.org/id/dataset/906780
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-08-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Acartia tonsa egg production rate and egg hatching success for transgenerational exposure to ocean warming and ocean acidification
2023-09-07
publication
2023-09-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-09-07
publication
https://doi.org/10.26008/1912/bco-dmo.906780.1
Hans G. Dam
University of Connecticut
principalInvestigator
Hannes Baumann
University of Connecticut
principalInvestigator
Michael Finiguerra
University of Connecticut
principalInvestigator
Melissa Pespeni
University of Vermont
principalInvestigator
Reid Brennan
University of Vermont
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: deMayo, J., Dam, H. G., Park, G., Norton, L., Finiguerra, M., Baumann, H., Brennan, R., Pespeni, M. (2023) Acartia tonsa egg production rate and egg hatching success for transgenerational exposure to ocean warming and ocean acidification. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.906780.1 [access date]
Ocean warming & acidification experiment: Acartia tonsa egg production and hatching success Dataset Description: <p>These data are part of at multigenerational experiment of Arcatia tonsa exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments.</p> Methods and Sampling: <p>For each replicate culture within a treatment, 10 pairs of newly developed males and females were placed into 20 mL petri dishes for 48 h (n = 280 per treatment). The dishes were housed in custom-made, airtight, plexiglass enclosures whose atmosphere was controlled to the appropriate CO<sub>2</sub> concentration. There was one enclosure per temperature-controlled incubator. After the 48-h egg laying period, adults were checked for survival and removed from the petri dishes. Eggs were left in the dishes for an additional 72 h to allow for egg hatching and their contents preserved with non-acid Lugol’s solution. Dishes with dead males were used for EPR, but not HS, since fertilization could not be assumed.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559075 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559075
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559180 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559180
completed
Hans G. Dam
University of Connecticut
860-405-9098
Dept. of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hans.dam@uconn.edu
pointOfContact
Hannes Baumann
University of Connecticut
860-405-9297
Department of Marine Sciences 1080 Shennecossett Road
Groton
CT
06340-6048
USA
hannes.baumann@uconn.edu
pointOfContact
Michael Finiguerra
University of Connecticut
860-405-9082
Department of Marine Sciences
Groton
CT
06340-6048
USA
michael.finiguerra@uconn.edu
pointOfContact
Melissa Pespeni
University of Vermont
802-656-0628
109 Carrigan Dr. Marsh Life Sciences 120A
Burlington
VT
05405
US
melissa.pespeni@uvm.edu
pointOfContact
Reid Brennan
University of Vermont
reid.brennan@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Generation
Rep
Treatment
Temp
pH
Hatched
Unhatched
Total
EPRtot
HFtot
Olympus SZH-ILLD stereo microscope
theme
None, User defined
stage
replicate
treatment
water temperature at measurement depth
pH
count
ratio
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
https://osprey.bco-dmo.org/project/706023
Collaborative Research: Response of marine copepods to warming temperature and ocean acidification
<p><strong><em>NSF Award Abstract:</em></strong><br />
Over time, our oceans are becoming both warmer and higher dissolved carbon dioxide. The latter condition is called ocean acidification. The consequences of these simultaneous changes for populations of marine organisms are not well understood. For this project, the investigators will conduct a series of laboratory experiments to determine how two closely-related, common species of Acartia copepods will respond to the interactive effects of warming and acidification and also how well these species can adapt over multiple generations to changing ocean conditions. Since these copepods are key species in coastal food webs, results will have important implications for understanding and predicting how marine ecosystems may respond to future climate change. The investigators will share results from the research through traditional print media, case studies, and video mini lectures. The goal will be for educators of all levels to easily access material on climate change and ocean acidification to include in teaching curricula, in alignment with recommendations for universal design for learning. The project is a collaborative effort between an established professor at the University of Connecticut and an early-career female scientist at the University of Vermont. It will provide training and opportunities for collaborative, interdisciplinary research for two postdoctoral investigators, two graduate students and an undergraduate student.</p>
<p>The project's main goals are: 1) to test the simultaneous effects of temperature and carbon dioxide under current and future conditions on life history traits throughout the life cycle for two key copepod species, warm-adapted Acartia tonsa and cold-adapted Acartia hudsonica; 2) to test for adaptive capacity of both copepod species to a warmer and carbon-dioxide-enriched ocean; 3) to measure the genetic and maternally-induced changes across multiple generations of experimental selection in future conditions in both copepod species, and to identify the genes and pathways responding to selection. The investigators will use experiments encompassing current and projected temperature and carbon-dioxide conditions, will determine the roles of each variable and their interaction on traits that affect the fitness of both copepod species. They will also determine which life stages are most sensitive to individual or simultaneous stress conditions. Through multigenerational selection experiments, the investigators will identify and characterize the mechanisms of copepod evolutionary adaptation. Finally, they will measure genomic changes across the generations under all four experimental conditions to quantify the relative contributions of genetic and maternally-induced change in the physiological and life history traits of copepods in response to near-future climate conditions.</p>
Copepod Response to Warming Temp and OA
largerWorkCitation
project
eng; USA
oceans
2023-09-07
North western Atlantic ocean; Gulf of Maine, coastal and estuarine habitats
0
BCO-DMO catalogue of parameters from Acartia tonsa egg production rate and egg hatching success for transgenerational exposure to ocean warming and ocean acidification
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/907074.rdf
Name: Generation
Units: unitless
Description: <p>The corresponding generation when survival was measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/907075.rdf
Name: Rep
Units: unitless
Description: <p>The corresponding treatment evaluated</p>
http://lod.bco-dmo.org/id/dataset-parameter/907076.rdf
Name: Treatment
Units: unitless
Description: <p>Target temperature for each treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/907077.rdf
Name: Temp
Units: degrees Celsius (°C)
Description: <p>Target pH for each treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/907078.rdf
Name: pH
Units: unitless
Description: <p>Biological culture replicate for each treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/907079.rdf
Name: Hatched
Units: unitless
Description: <p>Number of hatched eggs observed</p>
http://lod.bco-dmo.org/id/dataset-parameter/907080.rdf
Name: Unhatched
Units: unitless
Description: <p>Number of unhatched eggs observed</p>
http://lod.bco-dmo.org/id/dataset-parameter/907081.rdf
Name: Total
Units: unitless
Description: <p>Sum of "Hatched" and "Unhatched" columns</p>
http://lod.bco-dmo.org/id/dataset-parameter/907082.rdf
Name: EPRtot
Units: unitless
Description: <p>Rate of eggs produced per female per day</p>
http://lod.bco-dmo.org/id/dataset-parameter/907083.rdf
Name: HFtot
Units: unitless
Description: <p>Frequency of hatching success</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
32268
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/36fca9d2-3900-4680-94ea-43c99080571b/content
download
32268
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/36fca9d2-3900-4680-94ea-43c99080571b/content
download
https://doi.org/10.26008/1912/bco-dmo.906780.1
download
onLine
dataset
<p>For each replicate culture within a treatment, 10 pairs of newly developed males and females were placed into 20 mL petri dishes for 48 h (n = 280 per treatment). The dishes were housed in custom-made, airtight, plexiglass enclosures whose atmosphere was controlled to the appropriate CO<sub>2</sub> concentration. There was one enclosure per temperature-controlled incubator. After the 48-h egg laying period, adults were checked for survival and removed from the petri dishes. Eggs were left in the dishes for an additional 72 h to allow for egg hatching and their contents preserved with non-acid Lugol’s solution. Dishes with dead males were used for EPR, but not HS, since fertilization could not be assumed.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Dishes with dead females were discarded. EPR was calculated as (E<sub>u</sub>&nbsp;+ E<sub>h</sub>)/t<sub>&nbsp;</sub>where&nbsp;<em>E<sub>u</sub>&nbsp;</em>represents unhatched eggs,&nbsp;<em>E<sub>h</sub>&nbsp;</em>represents hatched eggs (nauplii), and&nbsp;<em>t</em>&nbsp;represents egg laying time. Hatching success was calculated as&nbsp;E<sub>h</sub>. All data was processed in R v4.0.2</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus SZH-ILLD stereo microscope
Olympus SZH-ILLD stereo microscope
PI Supplied Instrument Name: Olympus SZH-ILLD stereo microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/