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            <gco:CharacterString>Cite this dataset as: Lenz, P. H., Hartline, D. K., Monell, K. J. (2024) Post-diapause Neocalanus flemingeri females morphometric measurements and calculations of lipid fullness and lipid volume taken from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-06-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.907880.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sample collection and sorting&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Copepods were collected in Prince William Sound, Alaska in the summer and fall of 2019 during the Northern Gulf of Alaska Long Term Ecological Research (NGA LTER) cruises (&amp;lt;a href=&amp;quot;https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fnga.lternet.edu%2F&amp;amp;amp;data=05%7C02%7Clynne.merchant%40whoi.edu%7C57c61cd1b2744b33b18808dc4a1ee1a6%7Cd44c5cc6d18c46cc8abd4fdf5b6e5944%7C0%7C0%7C638466743718801708%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&amp;amp;amp;sdata=1DlNvufyEZ0jTuKJOBITrPrzzKroM2%2BLA0uHNcM0di4%3D&amp;amp;amp;reserved=0&amp;quot;&amp;gt;https://nga.lternet.edu/&amp;lt;/a&amp;gt;). Females “PWS2/June” were collected on June 30th, 2019 at the sampling site PWS2 (Latitude 60° 32.1’N; Longitude 147° 48.2’W) (R/V Sikuliaq, cruise number: SKQ201915S), and the “Pleiades/September” females were collected on September 12th&amp;amp;nbsp;&amp;amp;nbsp;and 13th, 2019 at PWS2 and near the Pleiades Islands (Latitude 60° 16.7’N; Longitude 147° 59.2’W) (M/V Tiglax, cruise number: TGX201909). Copepods were collected with a Midi MultiNet (0.25 m2&amp;amp;nbsp;mouth area; 150 μm mesh nets) towed vertically from near the bottom to the &amp;lt;s&amp;gt;163 &amp;lt;/s&amp;gt;surface at 0.5 m/sec (PWS2: 798 m; KIP2: 588 m). Upon retrieval, net samples were immediately diluted using filtered seawater collected from depth and kept between 4-6°C to minimize thermal stress. All females selected for the experiments were sorted under a dissecting microscope. Females were placed in groups of three into 750 mL Falcon tissue-culture flasks and incubated under dim light in an incubator for up to 4.5 weeks. Experimental emperatures were at or below deep-water temperatures in Prince William Sound (temperature settings: 4°C for June and 6°C for September). A subset of females was used in the DNA replication experiments; the remaining females were imaged for measurements of prosome length and lipid sac area (see the&amp;amp;nbsp;related dataset &amp;amp;nbsp;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/908514&amp;quot;&amp;gt;https://www.bco-dmo.org/dataset/908514&amp;lt;/a&amp;gt;).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Experimental design&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the timeline, sets of females were removed from the incubation flasks for imaging and/or incubations in low concentrations of 5-Ethynyl-2’-deoxyuridine (EdU). Individuals that were imaged as well as incubated in EdU are identified in the dataset and provide identifications used in the related dataset. &amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/542191.rdf" xlink:title="OCE-1459235" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459235 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459235</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/751106.rdf" xlink:title="OCE-1756767" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756767 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756767</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/751107.rdf" xlink:title="OCE-1756859" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756859 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756859</gmx:Anchor>
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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/562100.rdf" xlink:title="ORCID" xlink:actuate="onRequest">Daniel K. Hartline</gmx:Anchor>
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&lt;p&gt;The Gulf of Alaska supports a diverse and productive marine community that includes many commercially important fishes. Toward the base of this food web are small planktonic crustaceans that serve as the primary food source for many of these fish, as well as seabirds and marine mammals. The copepod Neocalanus flemingeri is one of these crustaceans, and it experiences rapid population growth during each spring's algal, or phytoplankton, bloom. An apparent mismatch between the presence of the youngest stages of the copepod, or nauplii, in early winter and the unpredictable timing of the spring phytoplankton bloom several months later raises important questions about when females reproduce and how this relates to survival and growth of nauplii. Two types of dormancy, diapause in adult females and physiological quiescence in nauplii, may be the key to the success of this copepod species. Timing and duration of the egg-laying period by adult females is linked to emergence from diapause. In addition, nauplii may enter a state of physiological quiescence while food resources are low, resuming growth after phytoplankton levels increase. This research will address a long-standing goal of biological oceanographers to understand dormancy and its role in controlling population cycles in marine copepods. It will use new technologies in molecular biology called transcriptomics to catalog the messages used by the cells to control copepod life processes, in this case those related to dormancy in adults and nauplii. Undergraduate students and a postdoctoral investigator will be trained in interdisciplinary research, and students from Native Hawaiian and Native Alaskan groups will be targeted for participation. Fishing is a major industry in the Gulf of Alaska, and outreach will focus on communicating the role copepods play in marine ecosystems. New content, including images, will be generated for existing websites: the Seward Line long-term observation program, the Alaska Ocean Observing System and the Gulf Watch Alaska Program.&lt;/p&gt;
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The sub-arctic Pacific sustains major fisheries with nearly all commercially important species depending either directly or indirectly on lipid-rich copepods (Neocalanus flemingeri, Neocalanus plumchrus, Neocalanus cristatus and Calanus marshallae). In turn, these species depend on a short-lived spring algal bloom for growth and the accumulation of lipid stores in order to complete an annual life cycle that includes a period of dormancy. The intellectual thrust of this project measures how the timing and magnitude of algal blooms affect preparation for dormancy using a combination of field and experimental observations. The Northern Gulf of Alaska - with four calanid species that experience dormancy, steep environmental gradients, well-described phytoplankton bloom dynamics, and a concurrent NSF-LTER program - provides an unusual opportunity to identify the factors that affect dormancy preparation. Education and outreach plans are integrated with the research. Educational efforts focus on interdisciplinary opportunities for undergraduate, graduate and post-doctoral trainees. The project will generate content for existing graduate and undergraduate courses. U. of Alaska Fairbanks and U. Hawaii at Manoa are Alaska Native and Native Hawaiian Serving Institutions, and students from these groups will be recruited to participate in the project. Because fishing is a major industry in the Gulf of Alaska, outreach will communicate the role copepods play in marine ecosystems using the concept of a dynamic food web tied to production cycles.&lt;/p&gt;
&lt;p&gt;Diapause (dormancy) and the accompanying accumulation of lipids in copepods have been identified as key drivers in high latitude ecosystems that support economically important fisheries, including those of the Gulf of Alaska. While the disappearance of lipid-rich copepods has been linked to severe declines in fish stocks, little is known about the environmental conditions that are required for the successful completion of the copepod's life cycle. A physiological profiling approach that measures relative gene expression will be used to test two alternative hypotheses: the lipid accumulation window hypothesis, which holds that individuals enter diapause only after they have accumulated sufficient lipid stores, and the developmental program hypothesis, which holds that once the diapause program is activated, progression occurs independent of lipid accumulation. The specific objectives are: 1) determine the effect of food levels during N. flemingeri copepodite stages on progression towards diapause using multiple physiological and developmental markers; 2) characterize the seasonal changes in the physiological profile of N. flemingeri across environmental gradients and across years; 3) compare physiological profiles across co-occurring calanid species (N. flemingeri, Neocalanus plumchrus, Neocalanus cristatus and Calanus marshallae); and 4) estimate the reproductive potential of the overwintering populations of N. flemingeri. The broader scientific significance includes the acquisition of new genomic data and molecular resources that will be made publicly available through established data repositories, and the development of new tools for routinely obtaining physiological profiles of copepods.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;
&lt;p&gt; &lt;/p&gt;
&lt;p&gt;&lt;strong&gt;NOTE:&lt;/strong&gt; Petra Lenz is a former Principal Investigator (PI) and Andrew Christie is a former Co-Principal Investigator (Co-PI) on this project (award #1756767). Daniel Hartline is the PI listed for the award #1756767 and is now a former Co-PI on this project.&lt;/p&gt;</gco:CharacterString>
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http://lod.bco-dmo.org/id/dataset-parameter/928928.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/928929.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/928931.rdf
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&amp;lt;p&amp;gt;Copepods were collected in Prince William Sound, Alaska in the summer and fall of 2019 during the Northern Gulf of Alaska Long Term Ecological Research (NGA LTER) cruises (&amp;lt;a href=&amp;quot;https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fnga.lternet.edu%2F&amp;amp;amp;data=05%7C02%7Clynne.merchant%40whoi.edu%7C57c61cd1b2744b33b18808dc4a1ee1a6%7Cd44c5cc6d18c46cc8abd4fdf5b6e5944%7C0%7C0%7C638466743718801708%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&amp;amp;amp;sdata=1DlNvufyEZ0jTuKJOBITrPrzzKroM2%2BLA0uHNcM0di4%3D&amp;amp;amp;reserved=0&amp;quot;&amp;gt;https://nga.lternet.edu/&amp;lt;/a&amp;gt;). Females “PWS2/June” were collected on June 30th, 2019 at the sampling site PWS2 (Latitude 60° 32.1’N; Longitude 147° 48.2’W) (R/V Sikuliaq, cruise number: SKQ201915S), and the “Pleiades/September” females were collected on September 12th&amp;amp;nbsp;&amp;amp;nbsp;and 13th, 2019 at PWS2 and near the Pleiades Islands (Latitude 60° 16.7’N; Longitude 147° 59.2’W) (M/V Tiglax, cruise number: TGX201909). Copepods were collected with a Midi MultiNet (0.25 m2&amp;amp;nbsp;mouth area; 150 μm mesh nets) towed vertically from near the bottom to the &amp;lt;s&amp;gt;163 &amp;lt;/s&amp;gt;surface at 0.5 m/sec (PWS2: 798 m; KIP2: 588 m). Upon retrieval, net samples were immediately diluted using filtered seawater collected from depth and kept between 4-6°C to minimize thermal stress. All females selected for the experiments were sorted under a dissecting microscope. Females were placed in groups of three into 750 mL Falcon tissue-culture flasks and incubated under dim light in an incubator for up to 4.5 weeks. Experimental emperatures were at or below deep-water temperatures in Prince William Sound (temperature settings: 4°C for June and 6°C for September). A subset of females was used in the DNA replication experiments; the remaining females were imaged for measurements of prosome length and lipid sac area (see the&amp;amp;nbsp;related dataset &amp;amp;nbsp;&amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/908514&amp;quot;&amp;gt;https://www.bco-dmo.org/dataset/908514&amp;lt;/a&amp;gt;).&amp;lt;/p&amp;gt;

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2. Renamed fields to follow BCO-DMO parameter naming conventions by replacing spaces with underscores and removing punctuation marks and parenthesis. 
3. Removed units in the parameter names which will be noted in parameter definitions on the dataset page.
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8. Added a parameter column named Sex with the value 'female'.
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