http://lod.bco-dmo.org/id/dataset/908026 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-09-12 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Reef seawater biogeochemistry data from samples collected in the Jardines de la Reina reef-system, Cuba in November of 2017 2023-10-26 publication 2023-10-26 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2023-10-31 publication https://doi.org/10.26008/1912/bco-dmo.908026.1 Amy Apprill Woods Hole Oceanographic Institution principalInvestigator Elizabeth Kujawinski Woods Hole Oceanographic Institution principalInvestigator Laura Gray Woods Hole Oceanographic Institution principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Apprill, A., Kujawinski, E., Gray, L. (2023) Reef seawater biogeochemistry data from samples collected in the Jardines de la Reina reef-system, Cuba in November of 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-10-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.908026.1 [access date] Dataset Description: &lt;p&gt;Funding Description:&lt;/p&gt; &lt;p&gt;Dalio Foundation (now ‘OceanX’) (awarded to Amy Apprill)&lt;br /&gt; National Science Foundation (OCE-1736288) (awarded to Amy Apprill)&lt;/p&gt; &lt;p&gt;Mass spectrometry samples were analyzed at the WHOI FT-MS Users’ Facility using instruments funded by the National Science Foundation (grant OCE-1058448 awarded to Elizabeth B. Kujawinski and Melissa Kido Soule) and the Simons Foundation (Award ID #509042, awarded to Elizabeth B. Kujawinski)&lt;/p&gt; Methods and Sampling: &lt;p&gt;Location:&amp;nbsp;Jardines de la Reina reef system south of the island of Cuba; General location: 20.8333° N, 78.9167° W&lt;/p&gt; &lt;div&gt;Sampling and analytical procedures:&amp;nbsp;&lt;/div&gt; &lt;div&gt;&amp;nbsp;&lt;/div&gt; &lt;div&gt;Reef seawater microbial biogeochemistry and extracellular metabolite compositions (see &quot;Related Datasets&quot; section) were surveyed at nine, shallow (6 – 14 m in depth) forereef sites during a cruise to Jardines de la Reina (JR), Cuba in November of 2017. Seawater was also collected from two surface ‘off reef’ sites (800 – 1600 m depth).&amp;nbsp;&lt;/div&gt; &lt;div&gt;At each reef, CTD (YSI Exo Sonde, Xylem Inc., Yellow Springs, OH, USA) casts were completed to measure the physicochemical properties of the water column.&amp;nbsp;Seawater samples were collected from surface and reef depth to enumerate the number of microbial cells (1 mL samples) and assess macronutrient concentrations (30 mL for inorganic macronutrients and 40 mL for organic carbon and total nitrogen samples) using a submersible groundwater pump.&amp;nbsp;Seawater samples (4L) were collected from reef depth and off reef samples for chlorophyll and phaeophytin analysis.&amp;nbsp;&lt;/div&gt; &lt;div&gt;Samples collected for total organic carbon and nitrogen analyses were acidified with 75 µL of concentrated phosphoric acid, capped, and stored at room temperature. Surface and reef depth seawater samples collected for analyses of inorganic macronutrient concentrations (30 mL) were immediately frozen. Seawater, collected for enumeration of &lt;em&gt;Prochlorococcus&lt;/em&gt;, &lt;em&gt;Synechococcus&lt;/em&gt;, picoeukaryotic cells, and unpigmented cells (heterotrophic bacteria and archaea) was fixed with paraformaldehyde (1% final volume), incubated at 4 °C in the dark for 30 minutes, frozen at -50 °C on the research vessel, and then stored at -80 °C prior to analysis.&lt;/div&gt; &lt;div&gt; &lt;p&gt;Non-purgeable total organic carbon (TOC, unfiltered), dissolved organic carbon (DOC, 0.2 µm filtered), total nitrogen (TN, unfiltered), and total dissolved nitrogen (TDN, 0.2 µm filtered) concentrations were analyzed using a Shimadzu TOC-VCSH total organic carbon analyzer (Hansell &amp;amp; Carlson, 2001) with a TNM-1 module. Inorganic macronutrient (phosphate, nitrite + nitrate, nitrite, ammonium, silicate) concentrations were measured with a continuous segmented flow system (as used in Apprill &amp;amp; Rappé, 2011). Nitrite was subtracted from the nitrite + nitrate concentrations to obtain the nitrate concentrations. Concentrations of total organic nitrogen were obtained by subtracting the sum of the inorganic nitrogen species (nitrite + nitrate and ammonium) from the total nitrogen concentrations per sample. If the measured concentrations fell beneath the detection limits of the instrument (ammonium = 0.02 M, phosphate = 0.01 M, nitrite + nitrate = 0.07 M, nitrite = 0.01 M), these measurements were removed from the analysis.&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;em&gt;A few erroneous data values related to bottle/sample misidentification were included in the publication Weber et al. (2020). The differences between values are minor. The original values in Weber et al. (2020) are listed below and the corrected values are included within the dataset published here.&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;Reef name: JR4B_surf&amp;nbsp;&lt;br /&gt; The value for PO4 was 0.18&amp;nbsp;&lt;br /&gt; The value for SI was 1.6&amp;nbsp;&lt;br /&gt; The value for NO2 was 0.04&amp;nbsp;&lt;br /&gt; The value for NH4 was 0.03&amp;nbsp;&lt;br /&gt; The value for TON was 2.83&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;Reef name: OR2_surf&amp;nbsp;&lt;br /&gt; The value for TN was 0.18&amp;nbsp;&lt;br /&gt; The value for TON was -0.012&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;/div&gt; &lt;div&gt;Acetone (90%) was used to extract Chlorophyll a and phaeophytin and the optical density (OD) values were measured on a calibrated spectrophotometer using standard optics (Lambda 18, Perkin Elmer, Waltham, MA, USA).&amp;nbsp; The concentration ratios of chlorophyll a to phaeophytin were calculated and incorporated into the analyses. To obtain cell counts, flow cytometry was conducted using a collinear analysis method and a UV wavelength of 488 nm on an Altra flow cytometer at the University of Hawaii. Each sample was divided so that pigmented, fluorescent cells and unpigmented cells could be run separately. Analyzed volume was 100 ul. Unpigmented cells were stained with Hoechst stain at a final concentration of 1 µg mL-1. Abundances of each cell type were estimated by binning populations using FlowJo (v. 6.4.7) software.&lt;/div&gt; &lt;div&gt;Surface and reef depth seawater samples were also collected to quantify the concentrations of known metabolites and survey trends in untargeted metabolite feature composition using liquid chromatography mass spectrometry. Detailed methods are included on the MetaboLights project page (Project MTBLS1820) and within the open access paper.&lt;/div&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736288 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736288 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1058448 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1058448 completed Amy Apprill Woods Hole Oceanographic Institution 508-289-2649 Marine Chemistry and Geochemistry Department 266 Woods Hole Road, MS# 4 Woods Hole MA 02543 USA aapprill@whoi.edu pointOfContact Elizabeth Kujawinski Woods Hole Oceanographic Institution 508-289-3493 266 Woods Hole Rd. MS #4 Woods Hole MA 02543 USA ekujawinski@whoi.edu pointOfContact Laura Gray Woods Hole Oceanographic Institution 508-289-2981; 301-602-1540 Marine Chemistry and Geochemistry Department 266 Woods Hole Road, Mailstop 4 Woods Hole MA 02543 USA laura.gray@whoi.edu pointOfContact asNeeded Dataset Version: 1 Unknown reef site date latitude longitude depth biome sampling_depth grouping DOC DN TOC TN Pro Syn Pico Hbact Totalcells PO4 SI NO2 NO3 NH4 DON TON Temp pH Sal DO Tchl TchltoPhaeo Altra flow cytometer Shimadzu TOC-VCSH total organic carbon analyzer (Hansell & Carlson, 2001) Lambda 18, Perkin Elmer, Waltham, MA, USA YSI Exo Sonde (Xylem Inc., Yellow Springs, OH, USA) theme None, User defined Site_ID site date latitude longitude depth site description dissolved organic Carbon Total Dissolved Nitrogen total organic Carbon Nitrogen prochlorococcus abundance synechococcus abundance pico_euks cell_concentration reactive phosphorus (PO4) Silicate, Si(OH)4, silicic acid Nitrite Nitrate Ammonium Dissolved Organic Nitrogen total organic Nitrogen water temperature at measurement depth pH salinity dissolved Oxygen chlorophyll a No BCO-DMO term featureType BCO-DMO Standard Parameters Flow Cytometer Shimadzu Total Organic Carbon Analyzer TOC-VCPH Spectrophotometer YSI EXO multiparameter water quality sondes instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Signature exometabolomes of Caribbean corals and influences on reef picoplankton https://osprey.bco-dmo.org/project/746196 Signature exometabolomes of Caribbean corals and influences on reef picoplankton <p><em>NSF Award Abstract:</em><br /> Coral reefs are some of the most diverse and productive ecosystems in the ocean. Globally, reefs have declined in stony (reef-building) coral abundance due to environmental variations, and in the Caribbean this decline has coincided with an increase in octocoral (soft coral) abundance. This phase shift occurring on Caribbean reefs may be impacting the interactions between the sea floor and water column and particularly between corals and picoplankton. Picoplankton are the microorganisms in the water column that utilize organic matter released from corals to support their growth. These coral-picoplankton interactions are relatively unstudied, but could have major implications for reef ecology and coral health. This project will take place in the U.S. territory of the Virgin Islands (USVI) and will produce the first detailed knowledge about the chemical diversity and composition of organic matter released from diverse stony coral and octocoral species. This project will advance our understanding of coral reef microbial ecology by allowing us to understand how different coral metabolites impact picoplankton growth and dynamics over time. The results from this project will be made publically accessible in a freely available online magazine, and USVI minority middle and high school students will be exposed to a lesson about chemical-biological interactions on coral reefs through established summer camps. This project will also contribute to the training of USVI minority undergraduates as well as a graduate student.</p> <p>Coral exometabolomes, which are the sum of metabolic products of the coral together with its microbiome, are thought to structure picoplankton communities in a species-specific manner. However, a detailed understanding of coral exometabolomes, and their influences on reef picoplankton, has not yet been obtained. This project will utilize controlled aquaria-based experiments with stony corals and octocorals, foundational species of Caribbean reef ecosystems, to examine how the exometabolomes of diverse coral species differentially influence the reef picoplankton community. Specifically, this project will capitalize on recent developments in mass spectrometry-based metabolomics to define the signature exometabolomes of ecologically important and diverse stony corals and octocorals. Secondly, this project will determine how the exometabolomes of these corals vary with factors linked to coral taxonomy as well as the coral-associated microbiome (Symbiodinium algae, bacteria and archaea). With this new understanding of coral exometabolomes, the project will then apply a stable isotope probe labeling approach to the coral exometabolome and will examine if and how (through changes in growth and activity) the seawater picoplankton community incorporates coral exometabolomes from different coral species over time. This project will advance our ability to evaluate the role that coral exometabolomes play in contributing to benthic-picoplankton interactions on changing Caribbean reefs.</p> Coral Exometabolomes largerWorkCitation project RAPID/MRI: Acquisition of a Triple-Quad Mass Spectrometer for Quantitative Identification of Dispersants and Water-Soluble Oil in the Gulf of Mexico https://osprey.bco-dmo.org/project/843277 RAPID/MRI: Acquisition of a Triple-Quad Mass Spectrometer for Quantitative Identification of Dispersants and Water-Soluble Oil in the Gulf of Mexico <p>The PI's request MRI RAPID funding to acquire a triple-quad Mass Spectrometer for quantitative identification of dispersants and water-soluble oil in the Gulf of Mexico. Dispersants were applied to the leak at the bottom of the ocean. Preliminary results using the PI's Fourier-transform ion cyclotron resonance (FT) mass spectrometer show that is possible identify the active ingredient of this dispersant in samples collected during research cruises in the Gulf of Mexico. Components of the dispersant have even been found in samples taken from within the underwater oil plume deep below the ocean's surface (~1100 m). Now the PI's would like to quantify this compound in order to assess its environmental fate in this environment.</p> <p>In order to quantify these marker compounds, a mass spectrometer designed for sensitive and accurate quantification of targeted compounds is required. The PI's have identified a triple-quadrupole mass spectrometer (triple-Q-MS) as the most appropriate instrument for their needs. With the help of the EPA, the PI's now have the appropriate method ready and have been running samples on a triple-Q-MS in a colleague's lab. The increased sensitivity and quantitative accuracy of the triple-Q-MS will allow them to quantify dispersant components and other target compounds at lower concentrations, thus providing important constraints on modeling and predictive efforts underway in other research groups.</p> <p>Broader Impacts</p> <p>This research has the potential to provided unprecedented data on the environmental fate of both petroleum and dispersant components as they interact with the extant biological, chemical, and physical processes of the Gulf of Mexico. Beyond the immediate needs of the Gulf oil spill the development of the methods described in the proposal will have broad applications not only in oil spill research but also in marine organic matter characterization and its interactions with biological, chemical and physical processes. The instrument will be available for Gulf oil spill related research in a timeframe consistent with the intent of the RAPID funding mechanism.</p> RAPID Mass Spec for Dispersants largerWorkCitation project eng; USA oceans -79.5911 -78.3811 20.5065 21.3033 2017-11-05 2017-11-20 From projects that focused on the following 2 locations: 1. U.S. Virgin Islands 2. Gulf of Mexico 0 BCO-DMO catalogue of parameters from Reef seawater biogeochemistry data from samples collected in the Jardines de la Reina reef-system, Cuba in November of 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/913816.rdf Name: reef Units: unitless Description: <p>sample name of reef (site identifier and sample depth; "surf" for surface or "benthic" for reef depth).</p> http://lod.bco-dmo.org/id/dataset-parameter/913817.rdf Name: site Units: unitless Description: <p>Site identifier</p> http://lod.bco-dmo.org/id/dataset-parameter/913818.rdf Name: date Units: unitless Description: <p>Date of water sample collection in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/913819.rdf Name: latitude Units: decimal degrees Description: <p>sample site latitude</p> http://lod.bco-dmo.org/id/dataset-parameter/913820.rdf Name: longitude Units: decimal degrees Description: <p>sample site longitude</p> http://lod.bco-dmo.org/id/dataset-parameter/913821.rdf Name: depth Units: meters (m) Description: <p>depth of sampling</p> http://lod.bco-dmo.org/id/dataset-parameter/913822.rdf Name: biome Units: unitless Description: <p>general habitat description</p> http://lod.bco-dmo.org/id/dataset-parameter/913823.rdf Name: sampling_depth Units: unitless Description: <p>Descriptive term for sampling depth; "surf" for surface or "benthic" for reef depth.</p> http://lod.bco-dmo.org/id/dataset-parameter/913824.rdf Name: grouping Units: unitless Description: <p>general geographic grouping of reef sites</p> http://lod.bco-dmo.org/id/dataset-parameter/913825.rdf Name: DOC Units: micro molar (uM) Description: <p>Dissolved organic carbon</p> http://lod.bco-dmo.org/id/dataset-parameter/913826.rdf Name: DN Units: micro molar (uM) Description: <p>Dissolved nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/913827.rdf Name: TOC Units: micro molar (uM) Description: <p>Total organic carbon</p> http://lod.bco-dmo.org/id/dataset-parameter/913828.rdf Name: TN Units: micro molar (uM) Description: <p>Total nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/913829.rdf Name: Pro Units: cells per mL Description: <p>Prochlorococcus cell abundance</p> http://lod.bco-dmo.org/id/dataset-parameter/913830.rdf Name: Syn Units: cells per mL Description: <p>Synechococcus cell abundance</p> http://lod.bco-dmo.org/id/dataset-parameter/913831.rdf Name: Pico Units: cells per mL Description: <p>Picoeukaryote cell abundance</p> http://lod.bco-dmo.org/id/dataset-parameter/913832.rdf Name: Hbact Units: cells per mL Description: <p>Unpigmented cell abundance</p> http://lod.bco-dmo.org/id/dataset-parameter/913833.rdf Name: Totalcells Units: cells per mL Description: <p>Summation of all cell types</p> http://lod.bco-dmo.org/id/dataset-parameter/913834.rdf Name: PO4 Units: micro molar (uM) Description: <p>Phosphate</p> http://lod.bco-dmo.org/id/dataset-parameter/913835.rdf Name: SI Units: micro molar (uM) Description: <p>Silicate</p> http://lod.bco-dmo.org/id/dataset-parameter/913836.rdf Name: NO2 Units: micro molar (uM) Description: <p>Nitrite</p> http://lod.bco-dmo.org/id/dataset-parameter/913837.rdf Name: NO3 Units: micro molar (uM) Description: <p>Nitrate</p> http://lod.bco-dmo.org/id/dataset-parameter/913838.rdf Name: NH4 Units: micro molar (uM) Description: <p>Ammonium</p> http://lod.bco-dmo.org/id/dataset-parameter/913839.rdf Name: DON Units: micro molar (uM) Description: <p>Dissolved organic nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/913840.rdf Name: TON Units: micro molar (uM) Description: <p>Total organic nitrogen</p> http://lod.bco-dmo.org/id/dataset-parameter/913841.rdf Name: Temp Units: degrees Celsius Description: <p>Seawater temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/913842.rdf Name: pH Units: pH scale Description: <p>pH</p> http://lod.bco-dmo.org/id/dataset-parameter/913843.rdf Name: Sal Units: parts per thousand (ppt) Description: <p>salinity</p> http://lod.bco-dmo.org/id/dataset-parameter/913844.rdf Name: DO Units: mg per L Description: <p>Dissolved oxygen</p> http://lod.bco-dmo.org/id/dataset-parameter/913845.rdf Name: Tchl Units: micrograms per L Description: <p>Total chlorophyll a</p> http://lod.bco-dmo.org/id/dataset-parameter/913846.rdf Name: TchltoPhaeo Units: unitless Description: <p>Ratio of total chlorophyll a to phaeophytin concentrations</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 3747 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/96d56933-3809-4064-8ddf-66df77179a61/content download https://doi.org/10.26008/1912/bco-dmo.908026.1 download onLine dataset &lt;p&gt;Location:&amp;nbsp;Jardines de la Reina reef system south of the island of Cuba; General location: 20.8333° N, 78.9167° W&lt;/p&gt; &lt;div&gt;Sampling and analytical procedures:&amp;nbsp;&lt;/div&gt; &lt;div&gt;&amp;nbsp;&lt;/div&gt; &lt;div&gt;Reef seawater microbial biogeochemistry and extracellular metabolite compositions (see &quot;Related Datasets&quot; section) were surveyed at nine, shallow (6 – 14 m in depth) forereef sites during a cruise to Jardines de la Reina (JR), Cuba in November of 2017. Seawater was also collected from two surface ‘off reef’ sites (800 – 1600 m depth).&amp;nbsp;&lt;/div&gt; &lt;div&gt;At each reef, CTD (YSI Exo Sonde, Xylem Inc., Yellow Springs, OH, USA) casts were completed to measure the physicochemical properties of the water column.&amp;nbsp;Seawater samples were collected from surface and reef depth to enumerate the number of microbial cells (1 mL samples) and assess macronutrient concentrations (30 mL for inorganic macronutrients and 40 mL for organic carbon and total nitrogen samples) using a submersible groundwater pump.&amp;nbsp;Seawater samples (4L) were collected from reef depth and off reef samples for chlorophyll and phaeophytin analysis.&amp;nbsp;&lt;/div&gt; &lt;div&gt;Samples collected for total organic carbon and nitrogen analyses were acidified with 75 µL of concentrated phosphoric acid, capped, and stored at room temperature. Surface and reef depth seawater samples collected for analyses of inorganic macronutrient concentrations (30 mL) were immediately frozen. Seawater, collected for enumeration of &lt;em&gt;Prochlorococcus&lt;/em&gt;, &lt;em&gt;Synechococcus&lt;/em&gt;, picoeukaryotic cells, and unpigmented cells (heterotrophic bacteria and archaea) was fixed with paraformaldehyde (1% final volume), incubated at 4 °C in the dark for 30 minutes, frozen at -50 °C on the research vessel, and then stored at -80 °C prior to analysis.&lt;/div&gt; &lt;div&gt; &lt;p&gt;Non-purgeable total organic carbon (TOC, unfiltered), dissolved organic carbon (DOC, 0.2 µm filtered), total nitrogen (TN, unfiltered), and total dissolved nitrogen (TDN, 0.2 µm filtered) concentrations were analyzed using a Shimadzu TOC-VCSH total organic carbon analyzer (Hansell &amp;amp; Carlson, 2001) with a TNM-1 module. Inorganic macronutrient (phosphate, nitrite + nitrate, nitrite, ammonium, silicate) concentrations were measured with a continuous segmented flow system (as used in Apprill &amp;amp; Rappé, 2011). Nitrite was subtracted from the nitrite + nitrate concentrations to obtain the nitrate concentrations. Concentrations of total organic nitrogen were obtained by subtracting the sum of the inorganic nitrogen species (nitrite + nitrate and ammonium) from the total nitrogen concentrations per sample. If the measured concentrations fell beneath the detection limits of the instrument (ammonium = 0.02 M, phosphate = 0.01 M, nitrite + nitrate = 0.07 M, nitrite = 0.01 M), these measurements were removed from the analysis.&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;em&gt;A few erroneous data values related to bottle/sample misidentification were included in the publication Weber et al. (2020). The differences between values are minor. The original values in Weber et al. (2020) are listed below and the corrected values are included within the dataset published here.&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;Reef name: JR4B_surf&amp;nbsp;&lt;br /&gt; The value for PO4 was 0.18&amp;nbsp;&lt;br /&gt; The value for SI was 1.6&amp;nbsp;&lt;br /&gt; The value for NO2 was 0.04&amp;nbsp;&lt;br /&gt; The value for NH4 was 0.03&amp;nbsp;&lt;br /&gt; The value for TON was 2.83&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;Reef name: OR2_surf&amp;nbsp;&lt;br /&gt; The value for TN was 0.18&amp;nbsp;&lt;br /&gt; The value for TON was -0.012&amp;nbsp;&lt;/em&gt;&lt;/p&gt; &lt;/div&gt; &lt;div&gt;Acetone (90%) was used to extract Chlorophyll a and phaeophytin and the optical density (OD) values were measured on a calibrated spectrophotometer using standard optics (Lambda 18, Perkin Elmer, Waltham, MA, USA).&amp;nbsp; The concentration ratios of chlorophyll a to phaeophytin were calculated and incorporated into the analyses. To obtain cell counts, flow cytometry was conducted using a collinear analysis method and a UV wavelength of 488 nm on an Altra flow cytometer at the University of Hawaii. Each sample was divided so that pigmented, fluorescent cells and unpigmented cells could be run separately. Analyzed volume was 100 ul. Unpigmented cells were stained with Hoechst stain at a final concentration of 1 µg mL-1. Abundances of each cell type were estimated by binning populations using FlowJo (v. 6.4.7) software.&lt;/div&gt; &lt;div&gt;Surface and reef depth seawater samples were also collected to quantify the concentrations of known metabolites and survey trends in untargeted metabolite feature composition using liquid chromatography mass spectrometry. Detailed methods are included on the MetaboLights project page (Project MTBLS1820) and within the open access paper.&lt;/div&gt; Specified by the Principal Investigator(s) &lt;p&gt;Abundances of each cell type were estimated by binning populations using FlowJo (v. 6.4.7) software.&lt;/p&gt; Specified by the Principal Investigator(s) Version 1: * 908026_v2_biogeochemistry.csv was imported into the BCO-DMO data system with values &quot;NaN&quot; as missing data values. This file version was submitted to BCO-DMO 2023-10-26. * The table was renamed 908026_v1_biogeochemistry in the BCO-DMO system since it will be the first published version of this dataset. ** Missing data values are displayed differently based on the file format you download.  They are blank in csv files, &quot;NaN&quot; in MatLab files, etc. * date format changed to ISO format Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Altra flow cytometer Altra flow cytometer PI Supplied Instrument Name: Altra flow cytometer PI Supplied Instrument Description:To obtain cell counts, flow cytometry was conducted using a collinear analyses method and a UV wavelength of 488 nm on an Altra flow cytometer at the University of Hawaii. Each sample was divided so that pigmented, fluorescent cells and unpigmented cells could be run separately.  Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Shimadzu TOC-VCSH total organic carbon analyzer (Hansell & Carlson, 2001) Shimadzu TOC-VCSH total organic carbon analyzer (Hansell & Carlson, 2001) PI Supplied Instrument Name: Shimadzu TOC-VCSH total organic carbon analyzer (Hansell & Carlson, 2001) PI Supplied Instrument Description:Non-purgeable total organic carbon (TOC, unfiltered), dissolved organic carbon (DOC, 0.2 µm filtered), total nitrogen (TN, unfiltered), and total dissolved nitrogen (TDN, 0.2 µm filtered) concentrations were analyzed using a Shimadzu TOC-VCSH total organic carbon analyzer (Hansell & Carlson, 2001) with a TNM-1 module.  Instrument Name: Shimadzu Total Organic Carbon Analyzer TOC-VCPH Instrument Short Name:TOC-VCPH Instrument Description: The Shimadzu Total Organic Carbon Analyzer TOC-VCPH is a PC-controlled, total organic carbon analyzer (high-sensitivity model), designed to measure total carbon (TC), inorganic carbon (IC), total organic carbon (TOC), and non-purgeable organic carbon (NPOC); an optional accessory enables the measurement of particulate organic carbon (POC) and total nitrogen (TN) as well. The instrument uses the 680 degrees Celsius combustion catalytic oxidation method to analyze aqueous samples, and optionally solid and gas samples. Lambda 18, Perkin Elmer, Waltham, MA, USA Lambda 18, Perkin Elmer, Waltham, MA, USA PI Supplied Instrument Name: Lambda 18, Perkin Elmer, Waltham, MA, USA PI Supplied Instrument Description:Acetone (90%) was used to extract Chlorophyll a and phaeophytin and the optical density (OD) values were measured on a calibrated spectrophotometer using standard optics (Lambda 18, Perkin Elmer, Waltham, MA, USA).   Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/ YSI Exo Sonde (Xylem Inc., Yellow Springs, OH, USA) YSI Exo Sonde (Xylem Inc., Yellow Springs, OH, USA) PI Supplied Instrument Name: YSI Exo Sonde (Xylem Inc., Yellow Springs, OH, USA) PI Supplied Instrument Description:At each reef, CTD casts were completed (YSI Exo Sonde, Xylem Inc., Yellow Springs, OH, USA) to measure the physicochemical properties of the water column.  Instrument Name: YSI EXO multiparameter water quality sondes Instrument Short Name:YSI EXO Instrument Description: Comprehensive multi-parameter, water-quality monitoring sondes designed for long-term monitoring, profiling and spot sampling. The EXO sondes are split into several categories: EXO1 Sonde, EXO2 Sonde, EXO3 Sonde. Each category has a slightly different design purpose with the EXO2 and EXO3 containing more sensor ports than the EXO1. Data are collected using up to four user-replaceable sensors and an integral pressure transducer. Users communicate with the sonde via a field cable to an EXO Handheld, via Bluetooth wireless connection to a PC, or a USB connection to a PC. Typical parameter specifications for relevant sensors include dissolved oxygen with ranges of 0-50 mg/l, with a resolution of +/- 0.1 mg/l, an accuracy of 1 percent of reading for values between 0-20 mg/l and an accuracy of +/- 5 percent of reading for values 20-50 mg/l. Temp ranges are from-5 to +50 degC, with an accuracy of +/- 0.001 degC. Conductivity has a range of 0-200 mS/cm, with an accuracy of +/-0.5 percent of reading + 0.001 mS/cm and a resolution of 0.0001 - 0.01 mS/cm.