http://lod.bco-dmo.org/id/dataset/908189 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-09-12 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Total lipid measurements for field-collected animals from the Gulf of Mexico Estuary near Port Aransas and Mud Island, Texas from 2020 to 2021 2023-09-12 publication 2023-09-12 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-01-17 publication https://doi.org/10.26008/1912/bco-dmo.908189.1 Lee A. Fuiman University of Texas - Marine Science Institute principalInvestigator Parvathi Nair University of Texas - Marine Science Institute principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Fuiman, L. A., Nair, P. (2024) Total lipid measurements for field-collected animals from the Gulf of Mexico Estuary near Port Aransas and Mud Island, Texas from 2020 to 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.908189.1 [access date] Methods and Sampling: &lt;p&gt;Location:&amp;nbsp;Gulf of Mexico Estuary near Port Aransas, Texas. FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).&lt;br /&gt; &lt;br /&gt; Methods &amp;amp; Sampling&lt;/p&gt; &lt;p&gt;Total lipids&amp;nbsp;were measured for samples of marine animals and fish eggs collected from the field. Plankton net (500-µm mesh) samples were sorted immediately after collection (live material).&amp;nbsp; Samples of jellyfish were collected using dip net. Animals were transferred to holding tanks to evacuate their guts for 4-5 hours. Fish were collected using cast net and seines. Fishes were immediately euthanized with lethal dose of MS-222. The euthanized fish were placed on ice and liver was collected. Each sample was rinsed twice in distilled water and frozen at -80°C for subsequent analysis. The time between collection and analysis ranged from 2 months to a year.&lt;/p&gt; &lt;p&gt;For total lipids, each sample was lyophilized, homogenized, and weighed. Total lipid was measured by the phosphosulphovanillin method (Barnes and Blackstock, 1973). Briefly, lipids were cold extracted from lyophilized and homogenized samples with 2:1 chloroform: methanol (v/v). A calibration curve was prepared by performing 1:2 serial dilutions on a cholesterol standard (Millipore-Sigma, Burlington, MA, USA) dissolved in 2:1 chloroform:methanol (v/v). Blank, standards, and extracted lipid samples were reacted with concentrated&amp;nbsp;sulphuric acid and vanillin&amp;nbsp;(vanillin in 4:1 85% phosphoric acid: water v/v) and were run in duplicate. Absorbance was measured using a Spectramax 190 Microplate Reader (Molecular Devices, San Jose, CA, USA) at a wavelength of 520&amp;nbsp;nm.&amp;nbsp;Total lipids were expressed as mg g−1&amp;nbsp;dry weight.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023618 completed Lee A. Fuiman University of Texas - Marine Science Institute 361-749-6795 University of Texas Marine Science Institute 750 Channel View Drive Port Aransas TX 78373 lee.fuiman@utexas.edu pointOfContact Parvathi Nair University of Texas - Marine Science Institute 361-749-6795 University of Texas Marine Science Institute 750 Channel View Drive Port Aransas TX 78373 parvathinair@utexas.edu pointOfContact asNeeded Dataset Version: 1 Unknown Taxon Common_name Sample_ID Site lat lon Date_collected Date_analyzed Tissue Primary_check Total_lipids Spectramax 190 Microplate Reader theme None, User defined taxon common_name sample identification site latitude longitude date sample description quality flag total lipids featureType BCO-DMO Standard Parameters microplate instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons https://osprey.bco-dmo.org/project/817943 Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons <p><strong>NSF Award Abstract:</strong><br /> <span style="font-size:14px"><span style="background-color:rgb(255, 255, 255)">Marine animals release extremely large numbers of eggs when they spawn. Most of these eggs are eaten by animals ranging from microscopic plankton to fish. Many egg consumers are smaller than the animals that released the eggs, representing a reversal of the usual food web. The consumption of eggs provides animals with highly nutritious molecules called essential fatty acids which are very concentrated in eggs. These essential fatty acids are important for the health of animals and the health of the whole ecosystem. When marine fishes form spawning aggregations to coordinate the timing and location of spawning, they release trillions of eggs. This results in an "egg boon" an immense but temporary concentration of highly nutritious fatty acids. This project combines field-based sampling with laboratory experiments to assess how fatty acids in the egg boons affect food webs. The project is determining whether consumption of eggs is beneficial to the condition of the egg consumers. New findings from this project are advancing the understanding of aquatic food webs and contributing to improved management of marine resources. For example, commercial harvest of fish can remove tons of fatty acids from an ecosystem by reducing egg boons and leading to cascading and unforeseen effects on those biological communities. The project is fostering the participation of women in science by substantially advancing the professional training of a female postdoctoral fellow. The project is supporting K-12 STEM education through inquiry-based and place-based programs for teachers and youth. Findings are being communicated to the public locally and nationally through participation in public lectures and contributions to the Science and the SeaTM radio program, podcast, and website.</span></span></p> <p><span style="background-color:rgb(255, 255, 255)">Super-abundances of eggs released in temporally and spatially discrete patches create pulsed nutritional resources for egg consumers, called "egg boons", which are potentially important components of marine food webs. While various marine animals have been shown to consume eggs, the role of egg boons in energy transfer through food webs has received little attention. Three hypotheses are being tested: 1) egg boons provide a pathway through which essential fatty acids (EFAs) are redistributed counter to the main direction of trophic flow; 2) stores of EFAs in egg consumers increase during egg boons and remain elevated after the spawning season; and 3) egg boons are beneficial to the condition of egg consumers. The proposed research takes advantage of an annual egg boon produced by a spawning aggregation of the marine fish, red drum (Sciaenops ocellatus) near Port Aransas, Texas. In a combination of field sampling and laboratory experiments, fatty acid profiles, lipid content, and bulk stable isotope ratios are measures used to define trophic links between the egg boon and a selection of lower-trophic-level taxa. Egg boons are simulated in laboratory feeding experiments that are designed to enhance interpretation of data collected from field based sampling by comparing taxa that consume fish eggs with those that do not. A nucleic acid biomarker (RNA/DNA ratios) is being used to assess changes in condition that can be attributed to egg consumption in target taxa. In the environment, the importance and persistence of counter-gradient flow of fatty acids in the food web is being gauged through comparisons of samples taken inside and outside the spatial and temporal extent of the egg boon. The effects of egg consumption on consumers is being quantified in controlled experiments to identify dietary biomarkers of egg consumption in consumer tissues that can be applied to field samples. The proposed research examines how egg consumption redistributes EFAs within food webs and provides a context for considering potential controls and trophic bottlenecks that cannot be explained from the traditional element-limitation models. The integration of fatty acid and stable isotope approaches is expected to provide greater resolution for tracking organic matter through food webs and to advance the application of multi-tracer techniques in trophic investigations. Further, if egg boons are indeed an important nutritional subsidy to select groups of consumers, then subsequent studies investigating the energetic contribution of egg boons to secondary production in marine food webs are warranted. An analysis of how reduction or removal of egg resources through the harvest of fishes in spawning aggregations changes nutrient flow in food webs could have implications for ecosystem-based fisheries management.</span></p> Egg Boon Food Webs largerWorkCitation project eng; USA oceans -97.0827 -97.0218 27.8396 27.9362 2020-07-04 2021-09-10 Gulf of Mexico estuary at Port Aransas, Texas 0 BCO-DMO catalogue of parameters from Total lipid measurements for field-collected animals from the Gulf of Mexico Estuary near Port Aransas and Mud Island, Texas from 2020 to 2021 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/916145.rdf Name: Taxon Units: unitless Description: <p>Taxonomic grouping of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/916146.rdf Name: Common_name Units: unitless Description: <p>Common name of sample</p> http://lod.bco-dmo.org/id/dataset-parameter/916147.rdf Name: Sample_ID Units: unitless Description: <p>Sample identifier for a taxon on a sampling date</p> http://lod.bco-dmo.org/id/dataset-parameter/916148.rdf Name: Site Units: unitless Description: <p>location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute; MI: Mud Island in Aransas Bay, TX, United States.</p> http://lod.bco-dmo.org/id/dataset-parameter/916149.rdf Name: lat Units: decimal degrees Description: <p>site latitude</p> http://lod.bco-dmo.org/id/dataset-parameter/916150.rdf Name: lon Units: decimal degrees Description: <p>site longitude</p> http://lod.bco-dmo.org/id/dataset-parameter/916151.rdf Name: Date_collected Units: unitless Description: <p>Date sample was collected. ISO 8601 format.</p> http://lod.bco-dmo.org/id/dataset-parameter/916152.rdf Name: Date_analyzed Units: unitless Description: <p>Date sample was analyzed. ISO 8601 format.</p> http://lod.bco-dmo.org/id/dataset-parameter/916153.rdf Name: Tissue Units: unitless Description: <p>Tissue sampled</p> http://lod.bco-dmo.org/id/dataset-parameter/916154.rdf Name: Primary_check Units: unitless Description: <p>Primany QC check</p> http://lod.bco-dmo.org/id/dataset-parameter/916155.rdf Name: Total_lipids Units: milligrams per gram dry weight (mg g-1 dw) Description: <p>Total lipids</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 35659 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/0cbef56d-614f-425f-8869-c616e691fb1d/content download https://doi.org/10.26008/1912/bco-dmo.908189.1 download onLine dataset &lt;p&gt;Location:&amp;nbsp;Gulf of Mexico Estuary near Port Aransas, Texas. FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).&lt;br /&gt; &lt;br /&gt; Methods &amp;amp; Sampling&lt;/p&gt; &lt;p&gt;Total lipids&amp;nbsp;were measured for samples of marine animals and fish eggs collected from the field. Plankton net (500-µm mesh) samples were sorted immediately after collection (live material).&amp;nbsp; Samples of jellyfish were collected using dip net. Animals were transferred to holding tanks to evacuate their guts for 4-5 hours. Fish were collected using cast net and seines. Fishes were immediately euthanized with lethal dose of MS-222. The euthanized fish were placed on ice and liver was collected. Each sample was rinsed twice in distilled water and frozen at -80°C for subsequent analysis. The time between collection and analysis ranged from 2 months to a year.&lt;/p&gt; &lt;p&gt;For total lipids, each sample was lyophilized, homogenized, and weighed. Total lipid was measured by the phosphosulphovanillin method (Barnes and Blackstock, 1973). Briefly, lipids were cold extracted from lyophilized and homogenized samples with 2:1 chloroform: methanol (v/v). A calibration curve was prepared by performing 1:2 serial dilutions on a cholesterol standard (Millipore-Sigma, Burlington, MA, USA) dissolved in 2:1 chloroform:methanol (v/v). Blank, standards, and extracted lipid samples were reacted with concentrated&amp;nbsp;sulphuric acid and vanillin&amp;nbsp;(vanillin in 4:1 85% phosphoric acid: water v/v) and were run in duplicate. Absorbance was measured using a Spectramax 190 Microplate Reader (Molecular Devices, San Jose, CA, USA) at a wavelength of 520&amp;nbsp;nm.&amp;nbsp;Total lipids were expressed as mg g−1&amp;nbsp;dry weight.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;A&amp;nbsp;calibration curve&amp;nbsp;was obtained by performing 7, 1:2 dilutions on standard stock solution. Standard stock solution was made by dissolving approximately 8 mg cholesterol in approximately 4 ml 2:1 chloroform:methanol. The measured absorbance was subtracted from the blank (2:1 chloroform:methanol), and the calibration curve was used to&amp;nbsp;calculate total lipids in&amp;nbsp;mg g−1dry weight.&lt;/p&gt; Specified by the Principal Investigator(s) BCO-DMO Data Manager Processing Notes: * Sheet 1 of file &quot;Total lipid field data.xlsx&quot; (submitted in our online submission system 2023-06-23) was imported into the BCO-DMO data system with values &quot;NA&quot; as missing data values.  ** Missing data values are displayed differently based on the file format you download.  They are blank in csv files, &quot;NaN&quot; in MatLab files, etc. * Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores.  Can not start with a number] * Taxon name and associated LSID for names in this dataset as of 2023-12-01 (source: World Register of Marine Species). Added this list to Methods and Sampling section. * lat and lon columns were added for sites from locations provided in metadata. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Spectramax 190 Microplate Reader Spectramax 190 Microplate Reader PI Supplied Instrument Name: Spectramax 190 Microplate Reader Instrument Name: microplate Instrument Short Name: Instrument Description: A flat dish with multiple individual wells that are arrayed in a standardized number, size, and arrangement.