http://lod.bco-dmo.org/id/dataset/908200
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-09-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and Nitrogen stable isotope measurements for experimental animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
2023-09-12
publication
2023-09-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-01-16
publication
https://doi.org/10.26008/1912/bco-dmo.908200.1
Lee A. Fuiman
University of Texas - Marine Science Institute
principalInvestigator
Parvathi Nair
University of Texas - Marine Science Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuiman, L. A., Nair, P. (2024) Carbon and Nitrogen stable isotope measurements for experimental animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.908200.1 [access date]
Dataset Description: <p>These data were published in&nbsp;Nair et al. (2023).</p> Methods and Sampling: Mnemiopsis leidyi and juvenile Callinectes similis were collected in March – April 2022, and Beroe ovata was collected in November 2022, using a plankton net (50 cm diameter, 500 μm mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W). Palaemon pugio was collected using a plankton net from Corpus Christi Bay (27.8035° N, 97.0898° W) in Port Aransas in July 2021. Opisthonema oglinum and Lagodon rhomboides were collected using a seine (6.4 m wide by 1.2 m high with 5 mm square mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W) in August 2021 and Redfish Bay at Aransas Pass (27.8611° N, 97.07632° W) in May 2022, respectively.
The live animals of each species were divided into two treatments (control and experimental). Both treatments were fed a common diet of either live Artemia sp. nauplii (enriched with Alga-Mac 3050; Aquafauna Bio-Marine, Inc.) or commercial fish food, Otohime (EP1, Reed Mariculture, Inc.) during the acclimation period of 10 – 45 days. After acclimation (Day 0), both treatments received a common diet of Artemia or Otohime, and the diet of experimental treatments was supplemented with red drum eggs for a period of 10 – 94 days. Controls did not receive eggs. Three to eight tanks of study species were sampled at the end of acclimation (day 0). Three to eight replicate tanks (N) were sampled from each treatment 24 h and 2 – 10 days after the experimental treatment received eggs.
Mnemiopsis leidyi, B. ovata, and C. similis were held in rectangular tanks (26.7 cm long x 16.5 cm high x 16.5 cm wide), and P. pugio and fishes were held in circular tanks (12 cm in diameter, 6.4 cm deep) with recirculating filtered water. Within each rectangular tank, individuals of C. similis were held separately in round plastic containers (106.7 cm in diameter, 43.2 cm deep) with perforated lids to prevent aggressive contact. For the same reason, individuals of L. rhomboides were kept in separate perforated cylindrical enclosures (30 cm in diameter, 45 cm high) within each circular tank. Excess food and solid waste were siphoned daily from all tanks, and complete water changes were performed in rectangular tanks every 2 – 4 days. Environmental conditions were measured daily and were constant throughout the experiment (temperature: 21 – 24°C, salinity: 28 – 35 ppt, and photoperiod: 12-h light and 12-h dark).
Invertebrates removed from both treatments on sampling days were kept in clean sea water overnight to evacuate their guts and were sacrificed the following morning. For taxa with low dry weight, i.e., ctenophores, 3 – 4 individuals from each tank were pooled together to make a replicate. A single individual per tank of C. similis, and three individuals of P. pugio (subsamples, n=3) per tank were removed at each sampling day. Invertebrates were analyzed whole, except for C. similis, for which the exoskeleton was excluded. On each sampling day, one fish per tank was removed and immediately euthanized with tricaine methanesulfonate (MS-222). Euthanized fish were placed on ice where a fillet of dorsal white muscle tissue were collected. Subsamples (n = 5 – 21) of diets provided to both treatments (i.e., red drum eggs, Artemia, and Otohime) were collected throughout the experiments for each taxon. All samples were rinsed twice in distilled water and frozen at -80°C until analysis.
A known subsample (0.5 – 0.7 mg of crustacean and fishes, 4.9 – 5.2 mg of M. leidyi, 1.0 – 1.2 mg of B. ovata) of lyophilized and homogenized tissue was weighed into a tin capsule at the UTMSI Core Isotope Facility. A Thermo Fisher Scientific Flash Elemental Analyzer-Isolink coupled to a Thermo Fisher Scientific Delta V Plus Isotope Ratio Mass Spectrometry (Thermo Fisher Scientific, Waltham, MA USA) in continuous-flow (Helium) mode was used to determine carbon and nitrogen isotopic compositions. Isotope analysis produced δ13C and δ15N values in permil or parts per thousand as well as percent carbon and percent nitrogen in the sample.
Experiments took place at the Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222) ; University of Texas Marine Science Institute Core Isotope facility (lat. 27.83669, lon. -97.05250)
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023618
completed
Lee A. Fuiman
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
lee.fuiman@utexas.edu
pointOfContact
Parvathi Nair
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
parvathinair@utexas.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Taxon
Tissue_sampled
Length
Tank_number
Acclimation_days
Days_after_acclimation
Treatment
Diet_fed
Notes
Primary_check
d13C
percent_C
d15N
percent_N
ThermoFisher Scientific EA-Isolink CNSOH element analyzer
ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS)
theme
None, User defined
taxon
sample description
length
tank
time_elapsed
treatment
comments
quality flag
d13C
Elemental carbon (C)
d15N
Nitrogen
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
https://osprey.bco-dmo.org/project/817943
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
<p><strong>NSF Award Abstract:</strong><br />
<span style="font-size:14px"><span style="background-color:rgb(255, 255, 255)">Marine animals release extremely large numbers of eggs when they spawn. Most of these eggs are eaten by animals ranging from microscopic plankton to fish. Many egg consumers are smaller than the animals that released the eggs, representing a reversal of the usual food web. The consumption of eggs provides animals with highly nutritious molecules called essential fatty acids which are very concentrated in eggs. These essential fatty acids are important for the health of animals and the health of the whole ecosystem. When marine fishes form spawning aggregations to coordinate the timing and location of spawning, they release trillions of eggs. This results in an "egg boon" an immense but temporary concentration of highly nutritious fatty acids. This project combines field-based sampling with laboratory experiments to assess how fatty acids in the egg boons affect food webs. The project is determining whether consumption of eggs is beneficial to the condition of the egg consumers. New findings from this project are advancing the understanding of aquatic food webs and contributing to improved management of marine resources. For example, commercial harvest of fish can remove tons of fatty acids from an ecosystem by reducing egg boons and leading to cascading and unforeseen effects on those biological communities. The project is fostering the participation of women in science by substantially advancing the professional training of a female postdoctoral fellow. The project is supporting K-12 STEM education through inquiry-based and place-based programs for teachers and youth. Findings are being communicated to the public locally and nationally through participation in public lectures and contributions to the Science and the SeaTM radio program, podcast, and website.</span></span></p>
<p><span style="background-color:rgb(255, 255, 255)">Super-abundances of eggs released in temporally and spatially discrete patches create pulsed nutritional resources for egg consumers, called "egg boons", which are potentially important components of marine food webs. While various marine animals have been shown to consume eggs, the role of egg boons in energy transfer through food webs has received little attention. Three hypotheses are being tested: 1) egg boons provide a pathway through which essential fatty acids (EFAs) are redistributed counter to the main direction of trophic flow; 2) stores of EFAs in egg consumers increase during egg boons and remain elevated after the spawning season; and 3) egg boons are beneficial to the condition of egg consumers. The proposed research takes advantage of an annual egg boon produced by a spawning aggregation of the marine fish, red drum (Sciaenops ocellatus) near Port Aransas, Texas. In a combination of field sampling and laboratory experiments, fatty acid profiles, lipid content, and bulk stable isotope ratios are measures used to define trophic links between the egg boon and a selection of lower-trophic-level taxa. Egg boons are simulated in laboratory feeding experiments that are designed to enhance interpretation of data collected from field based sampling by comparing taxa that consume fish eggs with those that do not. A nucleic acid biomarker (RNA/DNA ratios) is being used to assess changes in condition that can be attributed to egg consumption in target taxa. In the environment, the importance and persistence of counter-gradient flow of fatty acids in the food web is being gauged through comparisons of samples taken inside and outside the spatial and temporal extent of the egg boon. The effects of egg consumption on consumers is being quantified in controlled experiments to identify dietary biomarkers of egg consumption in consumer tissues that can be applied to field samples. The proposed research examines how egg consumption redistributes EFAs within food webs and provides a context for considering potential controls and trophic bottlenecks that cannot be explained from the traditional element-limitation models. The integration of fatty acid and stable isotope approaches is expected to provide greater resolution for tracking organic matter through food webs and to advance the application of multi-tracer techniques in trophic investigations. Further, if egg boons are indeed an important nutritional subsidy to select groups of consumers, then subsequent studies investigating the energetic contribution of egg boons to secondary production in marine food webs are warranted. An analysis of how reduction or removal of egg resources through the harvest of fishes in spawning aggregations changes nutrient flow in food webs could have implications for ecosystem-based fisheries management.</span></p>
Egg Boon Food Webs
largerWorkCitation
project
eng; USA
oceans
-97.0898
-97.0726
27.8035
27.8611
2020-07-01
2022-11-01
Gulf of Mexico estuary at Port Aransas, Texas
0
BCO-DMO catalogue of parameters from Carbon and Nitrogen stable isotope measurements for experimental animals used in laboratory-based experiments collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/915652.rdf
Name: Taxon
Units: unitless
Description: <p>Taxonomic grouping of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/915653.rdf
Name: Tissue_sampled
Units: unitless
Description: <p>Animal tissue sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/915654.rdf
Name: Length
Units: centimeters (cm)
Description: <p>Total length of fish; carapace length of crabs</p>
http://lod.bco-dmo.org/id/dataset-parameter/915655.rdf
Name: Tank_number
Units: unitless
Description: <p>Tank that animal was assigned to</p>
http://lod.bco-dmo.org/id/dataset-parameter/915656.rdf
Name: Acclimation_days
Units: days
Description: <p>Acclimation period began soon after animals were collected from the wild. During acclimation animals were fed Artemia or Otohime</p>
http://lod.bco-dmo.org/id/dataset-parameter/915657.rdf
Name: Days_after_acclimation
Units: days
Description: <p>Days in control or experimental treatment after acclimation. End of Acclimation marked by Day 0</p>
http://lod.bco-dmo.org/id/dataset-parameter/915658.rdf
Name: Treatment
Units: unitless
Description: <p>Control or Experimental</p>
http://lod.bco-dmo.org/id/dataset-parameter/915659.rdf
Name: Diet_fed
Units: unitless
Description: <p>Diets provided to control and experimental treatment. Controls were fed Artemia/Otohime only. Experimentals were fed Artemia/Otohime supplemented with red drum eggs</p>
http://lod.bco-dmo.org/id/dataset-parameter/915660.rdf
Name: Notes
Units: unitless
Description: <p>Notes about sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/915661.rdf
Name: Primary_check
Units: unitless
Description: <p>QC check</p>
http://lod.bco-dmo.org/id/dataset-parameter/915662.rdf
Name: d13C
Units: per mil
Description: <p>Stable isotope of carbon; values in permil or parts per thousand</p>
http://lod.bco-dmo.org/id/dataset-parameter/915663.rdf
Name: percent_C
Units: percentage
Description: <p>Percent carbon in the sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/915664.rdf
Name: d15N
Units: per mil
Description: <p>Stable isotope of nitrogen; values in permil or parts per thousand</p>
http://lod.bco-dmo.org/id/dataset-parameter/915665.rdf
Name: percent_N
Units: percentage
Description: <p>Percent nitrogen in the sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
23822
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4432a27f-e6f5-42c8-a014-5e9cc538cb64/content
download
https://doi.org/10.26008/1912/bco-dmo.908200.1
download
onLine
dataset
Mnemiopsis leidyi and juvenile Callinectes similis were collected in March – April 2022, and Beroe ovata was collected in November 2022, using a plankton net (50 cm diameter, 500 μm mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W). Palaemon pugio was collected using a plankton net from Corpus Christi Bay (27.8035° N, 97.0898° W) in Port Aransas in July 2021. Opisthonema oglinum and Lagodon rhomboides were collected using a seine (6.4 m wide by 1.2 m high with 5 mm square mesh) from Aransas Pass inlet at Port Aransas (27.8396° N, 97.0726° W) in August 2021 and Redfish Bay at Aransas Pass (27.8611° N, 97.07632° W) in May 2022, respectively.
The live animals of each species were divided into two treatments (control and experimental). Both treatments were fed a common diet of either live Artemia sp. nauplii (enriched with Alga-Mac 3050; Aquafauna Bio-Marine, Inc.) or commercial fish food, Otohime (EP1, Reed Mariculture, Inc.) during the acclimation period of 10 – 45 days. After acclimation (Day 0), both treatments received a common diet of Artemia or Otohime, and the diet of experimental treatments was supplemented with red drum eggs for a period of 10 – 94 days. Controls did not receive eggs. Three to eight tanks of study species were sampled at the end of acclimation (day 0). Three to eight replicate tanks (N) were sampled from each treatment 24 h and 2 – 10 days after the experimental treatment received eggs.
Mnemiopsis leidyi, B. ovata, and C. similis were held in rectangular tanks (26.7 cm long x 16.5 cm high x 16.5 cm wide), and P. pugio and fishes were held in circular tanks (12 cm in diameter, 6.4 cm deep) with recirculating filtered water. Within each rectangular tank, individuals of C. similis were held separately in round plastic containers (106.7 cm in diameter, 43.2 cm deep) with perforated lids to prevent aggressive contact. For the same reason, individuals of L. rhomboides were kept in separate perforated cylindrical enclosures (30 cm in diameter, 45 cm high) within each circular tank. Excess food and solid waste were siphoned daily from all tanks, and complete water changes were performed in rectangular tanks every 2 – 4 days. Environmental conditions were measured daily and were constant throughout the experiment (temperature: 21 – 24°C, salinity: 28 – 35 ppt, and photoperiod: 12-h light and 12-h dark).
Invertebrates removed from both treatments on sampling days were kept in clean sea water overnight to evacuate their guts and were sacrificed the following morning. For taxa with low dry weight, i.e., ctenophores, 3 – 4 individuals from each tank were pooled together to make a replicate. A single individual per tank of C. similis, and three individuals of P. pugio (subsamples, n=3) per tank were removed at each sampling day. Invertebrates were analyzed whole, except for C. similis, for which the exoskeleton was excluded. On each sampling day, one fish per tank was removed and immediately euthanized with tricaine methanesulfonate (MS-222). Euthanized fish were placed on ice where a fillet of dorsal white muscle tissue were collected. Subsamples (n = 5 – 21) of diets provided to both treatments (i.e., red drum eggs, Artemia, and Otohime) were collected throughout the experiments for each taxon. All samples were rinsed twice in distilled water and frozen at -80°C until analysis.
A known subsample (0.5 – 0.7 mg of crustacean and fishes, 4.9 – 5.2 mg of M. leidyi, 1.0 – 1.2 mg of B. ovata) of lyophilized and homogenized tissue was weighed into a tin capsule at the UTMSI Core Isotope Facility. A Thermo Fisher Scientific Flash Elemental Analyzer-Isolink coupled to a Thermo Fisher Scientific Delta V Plus Isotope Ratio Mass Spectrometry (Thermo Fisher Scientific, Waltham, MA USA) in continuous-flow (Helium) mode was used to determine carbon and nitrogen isotopic compositions. Isotope analysis produced δ13C and δ15N values in permil or parts per thousand as well as percent carbon and percent nitrogen in the sample.
Experiments took place at the Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222) ; University of Texas Marine Science Institute Core Isotope facility (lat. 27.83669, lon. -97.05250)
Specified by the Principal Investigator(s)
<p>ISODAT software (version 3.0) was used to collect the isotope trace chromatography and calculate raw isotopic ratios. Isotope results are presented using the conventional δ-notation:</p>
<p>δ<sup>13</sup>C (or δ<sup>15</sup>N)&nbsp; = [(R<sub>sample</sub>/R<sub>standard</sub>)-1] (in ‰)</p>
<p>where, R<sub>sample</sub>&nbsp;and R<sub>standard</sub>&nbsp;=&nbsp;<sup>13</sup>C/<sup>12</sup>C (or&nbsp;<sup>15</sup>N/<sup>14</sup>N). All δ-values are reported relative to VPDB for carbon and AIR for nitrogen, unless otherwise stated (Coplen, 1996). A two-point calibration of δ<sup>13</sup>C to VPDB&nbsp;and to δ15N to AIR was achieved using USGS-40 (−26.39‰,&nbsp;−4.52‰) and USGS-41a (+36.55‰, +47.55‰), respectively. Internal laboratory standards; casein (protein), was used to evaluate the carbon and nitrogen accuracy and precision during analytical sessions. Overall, carbon and nitrogen isotope results had a standard deviation of less than 0.2‰.</p>
Specified by the Principal Investigator(s)
BCO-DMO Data Manager Processing Notes:
* Sheet 1 of file "Stable isotope experimentals.xlsx" (submitted in our online submission system 2023-06-23) was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Taxon name and associated LSID for names in this dataset as of 2023-06-23 (source: World Register of Marine Species). Added this list to Methods and Sampling section.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ThermoFisher Scientific EA-Isolink CNSOH element analyzer
ThermoFisher Scientific EA-Isolink CNSOH element analyzer
PI Supplied Instrument Name: ThermoFisher Scientific EA-Isolink CNSOH element analyzer PI Supplied Instrument Description:ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS) coupled to a ThermoFisher Scientific EA-Isolink CNSOH element analyzer. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS)
ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS)
PI Supplied Instrument Name: ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Description:ThermoFisher Scientific Delta V isotope ratio mass spectrometer (IRMS) coupled to a ThermoFisher Scientific EA-Isolink CNSOH element analyzer. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/