http://lod.bco-dmo.org/id/dataset/908698
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-09-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022 as well as of red drum eggs spawned from captive adults from 2020 to 2022
2023-09-19
publication
2023-09-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-01-17
publication
https://doi.org/10.26008/1912/bco-dmo.908698.1
Lee A. Fuiman
University of Texas - Marine Science Institute
principalInvestigator
Parvathi Nair
University of Texas - Marine Science Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuiman, L. A., Nair, P. (2024) Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022 as well as of red drum eggs spawned from captive adults from 2020 to 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-09-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.908698.1 [access date]
Methods and Sampling: <p>Location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777); TPWD: Texas Parks and Wildlife Department, Corpus Christi, TX, United States (lat. 27.646877, lon. -97.3150222))<br />
<br />
Methods and Sampling:</p>
<p>A sampling of likely egg consumers, egg non-consumers, pelagic plankton, and benthic sediments began in July 2020 and ended in March 2022. Samples were collected from two field sites, one in the vicinity of the Red Drum spawning aggregation (Aransas Pass Inlet) and the other outside the extent of the egg boon (Mud Island in Aransas Bay) by deploying a 500-micron (µm) mesh plankton net.&nbsp; Animals in the plankton net were sorted immediately after collection and transferred to holding tanks to evacuate their guts for 3-4 hours. Each sample was then rinsed twice in distilled water and frozen at -80°C for subsequent fatty acid analysis.</p>
<p>Basal resources were collected by pumping seawater from the Aransas Pass Channel adjacent to the University of Texas Marine Science Institute’s Fisheries and Mariculture Laboratory and filtering the seawater through sieves of three different mesh sizes to obtain three size fractions of plankton samples (10-60 µm, 100-200 µm, and 200-500 µm). Bottom sediment was collected using a grab sampler and passed through a 60‑µm sieve to obtain benthic particulate organic matter. Samples of jellyfish and small fishes were taken with dip nets, cast nets, or seines and processed as described above for analysis.</p>
<p>Fatty acid profiles&nbsp;were measured for samples of fish eggs, marine animals, and basal resources collected from the field as well as eggs and marine animals used in laboratory experiments. The time between collection and analysis ranged from 2 months to a year.</p>
<p>For fatty acid analysis, each sample was lyophilized, homogenized, and weighed. Lipids were cold extracted from a known subsample (by weight) of the homogenized and lyophilized tissue using chloroform/methanol/water (8:4:3 v/v) following the method of Folch et al. (1957. Journal of Biological Chemistry 226:497–507) with 0.01% (w/v) butylated hydroxytoluene as an antioxidant. For small taxa or taxa with low lipid content (e.g., ctenophores), several individuals were pooled into one sample. Fatty acid methyl esters (FAME) were prepared by transesterification with 14% boron trifluoride in methanol (Morrison and Smith 1964. Journal of Lipid Research 5:600–608) following saponification of the total lipid using 0.5M potassium hydroxide in methanol. FAMEs were measured by gas chromatography with flame ionization detector following procedures described by Faulk and Holt (2005. Aquaculture 249:231–243). Measurements were expressed in terms of concentration (mg per g dry weight) and composition (% of total fatty acids). Fatty acids were identified by comparison with commercial standards.</p>
<p>Known Issues:<br />
Quality control procedure&nbsp;&nbsp;<br />
Principal components analysis for each taxon was performed on the mg g-1 dw and percent total fatty acids data, separately.&nbsp;&nbsp; Individual samples for which the score on the first or second principal component axis were greater than 4 standard deviations from the taxon mean were removed from the data set.&nbsp;</p>
<p>A primary check value was assigned as follows:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Perfectly fine</p>
<p>2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Data not evaluated because of too few data points for principal components analysis</p>
<p>The quantification limit for individual fatty acids is 0.013 mg g-1 dry weight</p>
<p>Measured values of any fatty acid less than 0.013 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%.</p>
<p>FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0727222);&nbsp;</p>
<p>MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2023618 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2023618
completed
Lee A. Fuiman
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
lee.fuiman@utexas.edu
pointOfContact
Parvathi Nair
University of Texas - Marine Science Institute
361-749-6795
University of Texas Marine Science Institute 750 Channel View Drive
Port Aransas
TX
78373
parvathinair@utexas.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Taxon
Common_name
Sample_ID
Year
Month
Date_collected
Date_analyzed
Classification
Notes
Site
lat
lon
Length
Primary_check
C14_pt_0_mg
C15_pt_0_mg
C16_pt_0_mg
C16_pt_1n7_mg
C16_pt_2n4_mg
C17_pt_0_mg
C16_pt_3n4_mg
C17_pt_1_mg
C18_pt_0_mg
C18_pt_1n9_mg
C18_pt_1n7_mg
C18_pt_2n6_mg
C18_pt_3n6_mg
C18_pt_3n4_mg
C18_pt_3n3_mg
C18_pt_4n3_mg
C20_pt_1n9_mg
C20_pt_2n6_mg
C20_pt_3n6_mg
C20_pt_4n6_mg
C20_pt_3n3_mg
C20_pt_4n3_mg
C20_pt_5n3_mg
C22_pt_1n11_mg
C22_pt_4n6_mg
C22_pt_5n6_mg
C22_pt_5n3_mg
C22_pt_6n3_mg
C20_pt_0_mg
Ci_15_pt_0_mg
C14_pt_1_mg
Ci_17_pt_0_mg
Ca_15_pt_0_mg
Ci_16_pt_0_mg
C14_pt_0_pct
C15_pt_0_pct
C16_pt_0_pct
C16_pt_1n7_pct
C16_pt_2n4_pct
C17_pt_0_pct
C16_pt_3n4_pct
C17_pt_1_pct
C18_pt_0_pct
C18_pt_1n9_pct
C18_pt_1n7_pct
C18_pt_2n6_pct
C18_pt_3n6_pct
C18_pt_3n4_pct
C18_pt_3n3_pct
C18_pt_4n3_pct
C20_pt_1n9_pct
C20_pt_2n6_pct
C20_pt_3n6_pct
C20_pt_4n6_pct
C20_pt_3n3_pct
C20_pt_4n3_pct
C20_pt_5n3_pct
C22_pt_1n11_pct
C22_pt_4n6_pct
C22_pt_5n6_pct
C22_pt_5n3_pct
C22_pt_6n3_pct
C20_pt_0_pct
Ci_15_pt_0_pct
C14_pt_1_pct
Ci_17_pt_0_pct
Ca_15_pt_0_pct
Ci_16_pt_0_pct
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
theme
None, User defined
taxon
common_name
sample identification
year
month of year
date
animal group (category)
comments
site
latitude
longitude
length
quality flag
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
https://osprey.bco-dmo.org/project/817943
Counter-gradient Flow of Fatty Acids in Marine Food Webs Through Egg Boons
<p><strong>NSF Award Abstract:</strong><br />
<span style="font-size:14px"><span style="background-color:rgb(255, 255, 255)">Marine animals release extremely large numbers of eggs when they spawn. Most of these eggs are eaten by animals ranging from microscopic plankton to fish. Many egg consumers are smaller than the animals that released the eggs, representing a reversal of the usual food web. The consumption of eggs provides animals with highly nutritious molecules called essential fatty acids which are very concentrated in eggs. These essential fatty acids are important for the health of animals and the health of the whole ecosystem. When marine fishes form spawning aggregations to coordinate the timing and location of spawning, they release trillions of eggs. This results in an "egg boon" an immense but temporary concentration of highly nutritious fatty acids. This project combines field-based sampling with laboratory experiments to assess how fatty acids in the egg boons affect food webs. The project is determining whether consumption of eggs is beneficial to the condition of the egg consumers. New findings from this project are advancing the understanding of aquatic food webs and contributing to improved management of marine resources. For example, commercial harvest of fish can remove tons of fatty acids from an ecosystem by reducing egg boons and leading to cascading and unforeseen effects on those biological communities. The project is fostering the participation of women in science by substantially advancing the professional training of a female postdoctoral fellow. The project is supporting K-12 STEM education through inquiry-based and place-based programs for teachers and youth. Findings are being communicated to the public locally and nationally through participation in public lectures and contributions to the Science and the SeaTM radio program, podcast, and website.</span></span></p>
<p><span style="background-color:rgb(255, 255, 255)">Super-abundances of eggs released in temporally and spatially discrete patches create pulsed nutritional resources for egg consumers, called "egg boons", which are potentially important components of marine food webs. While various marine animals have been shown to consume eggs, the role of egg boons in energy transfer through food webs has received little attention. Three hypotheses are being tested: 1) egg boons provide a pathway through which essential fatty acids (EFAs) are redistributed counter to the main direction of trophic flow; 2) stores of EFAs in egg consumers increase during egg boons and remain elevated after the spawning season; and 3) egg boons are beneficial to the condition of egg consumers. The proposed research takes advantage of an annual egg boon produced by a spawning aggregation of the marine fish, red drum (Sciaenops ocellatus) near Port Aransas, Texas. In a combination of field sampling and laboratory experiments, fatty acid profiles, lipid content, and bulk stable isotope ratios are measures used to define trophic links between the egg boon and a selection of lower-trophic-level taxa. Egg boons are simulated in laboratory feeding experiments that are designed to enhance interpretation of data collected from field based sampling by comparing taxa that consume fish eggs with those that do not. A nucleic acid biomarker (RNA/DNA ratios) is being used to assess changes in condition that can be attributed to egg consumption in target taxa. In the environment, the importance and persistence of counter-gradient flow of fatty acids in the food web is being gauged through comparisons of samples taken inside and outside the spatial and temporal extent of the egg boon. The effects of egg consumption on consumers is being quantified in controlled experiments to identify dietary biomarkers of egg consumption in consumer tissues that can be applied to field samples. The proposed research examines how egg consumption redistributes EFAs within food webs and provides a context for considering potential controls and trophic bottlenecks that cannot be explained from the traditional element-limitation models. The integration of fatty acid and stable isotope approaches is expected to provide greater resolution for tracking organic matter through food webs and to advance the application of multi-tracer techniques in trophic investigations. Further, if egg boons are indeed an important nutritional subsidy to select groups of consumers, then subsequent studies investigating the energetic contribution of egg boons to secondary production in marine food webs are warranted. An analysis of how reduction or removal of egg resources through the harvest of fishes in spawning aggregations changes nutrient flow in food webs could have implications for ecosystem-based fisheries management.</span></p>
Egg Boon Food Webs
largerWorkCitation
project
eng; USA
oceans
-97.3150222
-97.0218
27.6469
27.9362
2020-07-01
2023-03-21
Gulf of Mexico estuary at Port Aransas, Texas
0
BCO-DMO catalogue of parameters from Fatty acid measurements for various marine samples collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022 as well as of red drum eggs spawned from captive adults from 2020 to 2022
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/916158.rdf
Name: Taxon
Units: unitless
Description: <p>Taxonomic grouping of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/916159.rdf
Name: Common_name
Units: unitless
Description: <p>Common name of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/916160.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identifier for a taxon on a sampling date</p>
http://lod.bco-dmo.org/id/dataset-parameter/916161.rdf
Name: Year
Units: unitless
Description: <p>Year sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/916162.rdf
Name: Month
Units: unitless
Description: <p>Month sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/916163.rdf
Name: Date_collected
Units: unitless
Description: <p>Date sample was collected. ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/916164.rdf
Name: Date_analyzed
Units: unitless
Description: <p>Date sample was collected. ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/916165.rdf
Name: Classification
Units: unitless
Description: <p>Taxonomic group to which sample belongs</p>
http://lod.bco-dmo.org/id/dataset-parameter/916166.rdf
Name: Notes
Units: unitless
Description: <p>Notes about sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/916167.rdf
Name: Site
Units: unitless
Description: <p>Location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute; MI: Mud Island in Aransas Bay, TX, United States; TPWD: Texas Parks and Wildlife Department, Corpus Christi, TX, United States.</p>
http://lod.bco-dmo.org/id/dataset-parameter/916168.rdf
Name: lat
Units: decimal degrees
Description: <p>Site latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/916169.rdf
Name: lon
Units: decimal degrees
Description: <p>Site longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/916170.rdf
Name: Length
Units: centimeters (cm)
Description: <p>Bell diameter in centimeter for cnidarians; total length in centimeter for fish; carapace length in centimeter for crabs</p>
http://lod.bco-dmo.org/id/dataset-parameter/916171.rdf
Name: Primary_check
Units: unitless
Description: <p>Primary QC check</p>
http://lod.bco-dmo.org/id/dataset-parameter/916172.rdf
Name: C14_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 14:0 ; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916173.rdf
Name: C15_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916174.rdf
Name: C16_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916175.rdf
Name: C16_pt_1n7_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916176.rdf
Name: C16_pt_2n4_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:2n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916177.rdf
Name: C17_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916178.rdf
Name: C16_pt_3n4_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 16:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916179.rdf
Name: C17_pt_1_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 17:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916180.rdf
Name: C18_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916181.rdf
Name: C18_pt_1n9_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916182.rdf
Name: C18_pt_1n7_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916183.rdf
Name: C18_pt_2n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916184.rdf
Name: C18_pt_3n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916185.rdf
Name: C18_pt_3n4_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916186.rdf
Name: C18_pt_3n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916187.rdf
Name: C18_pt_4n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 18:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916188.rdf
Name: C20_pt_1n9_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916189.rdf
Name: C20_pt_2n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916190.rdf
Name: C20_pt_3n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916191.rdf
Name: C20_pt_4n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916192.rdf
Name: C20_pt_3n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916193.rdf
Name: C20_pt_4n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916194.rdf
Name: C20_pt_5n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916195.rdf
Name: C22_pt_1n11_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:1n-11; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916196.rdf
Name: C22_pt_4n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916197.rdf
Name: C22_pt_5n6_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:5n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916198.rdf
Name: C22_pt_5n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916199.rdf
Name: C22_pt_6n3_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 22:6n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916200.rdf
Name: C20_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 20:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916201.rdf
Name: Ci_15_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916202.rdf
Name: C14_pt_1_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid 14:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916203.rdf
Name: Ci_17_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso-17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916204.rdf
Name: Ca_15_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid anteiso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916205.rdf
Name: Ci_16_pt_0_mg
Units: milligrams per gram dry weight (mg g-1 dw)
Description: <p>measured value of fatty acid iso-16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916206.rdf
Name: C14_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 14:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916207.rdf
Name: C15_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916208.rdf
Name: C16_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916209.rdf
Name: C16_pt_1n7_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 16:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916210.rdf
Name: C16_pt_2n4_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 16:2n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916211.rdf
Name: C17_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916212.rdf
Name: C16_pt_3n4_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 16:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916213.rdf
Name: C17_pt_1_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 17:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916214.rdf
Name: C18_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916215.rdf
Name: C18_pt_1n9_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916216.rdf
Name: C18_pt_1n7_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:1n-7; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916217.rdf
Name: C18_pt_2n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916218.rdf
Name: C18_pt_3n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916219.rdf
Name: C18_pt_3n4_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:3n-4; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916220.rdf
Name: C18_pt_3n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916221.rdf
Name: C18_pt_4n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 18:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916222.rdf
Name: C20_pt_1n9_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:1n-9; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916223.rdf
Name: C20_pt_2n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:2n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916224.rdf
Name: C20_pt_3n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:3n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916225.rdf
Name: C20_pt_4n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916226.rdf
Name: C20_pt_3n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:3n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916227.rdf
Name: C20_pt_4n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:4n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916228.rdf
Name: C20_pt_5n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916229.rdf
Name: C22_pt_1n11_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 22:1n-11; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916230.rdf
Name: C22_pt_4n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 22:4n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916231.rdf
Name: C22_pt_5n6_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 22:5n-6; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916232.rdf
Name: C22_pt_5n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 22:5n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916233.rdf
Name: C22_pt_6n3_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 22:6n-3; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916234.rdf
Name: C20_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 20:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916235.rdf
Name: Ci_15_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid iso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916236.rdf
Name: C14_pt_1_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid 14:1; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916237.rdf
Name: Ci_17_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid iso-17:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916238.rdf
Name: Ca_15_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid anteiso-15:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
http://lod.bco-dmo.org/id/dataset-parameter/916239.rdf
Name: Ci_16_pt_0_pct
Units: % total fatty acids
Description: <p>measured value of fatty acid iso-16:0; quantification limit is 0.00044 mg g-1 dry weight; Measured values less than 0.00044 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
568392
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/0da6aff5-4b82-425d-8504-f54e93065368/content
download
https://doi.org/10.26008/1912/bco-dmo.908698.1
download
onLine
dataset
<p>Location where sample was collected (FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0827222); MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777); TPWD: Texas Parks and Wildlife Department, Corpus Christi, TX, United States (lat. 27.646877, lon. -97.3150222))<br />
<br />
Methods and Sampling:</p>
<p>A sampling of likely egg consumers, egg non-consumers, pelagic plankton, and benthic sediments began in July 2020 and ended in March 2022. Samples were collected from two field sites, one in the vicinity of the Red Drum spawning aggregation (Aransas Pass Inlet) and the other outside the extent of the egg boon (Mud Island in Aransas Bay) by deploying a 500-micron (µm) mesh plankton net.&nbsp; Animals in the plankton net were sorted immediately after collection and transferred to holding tanks to evacuate their guts for 3-4 hours. Each sample was then rinsed twice in distilled water and frozen at -80°C for subsequent fatty acid analysis.</p>
<p>Basal resources were collected by pumping seawater from the Aransas Pass Channel adjacent to the University of Texas Marine Science Institute’s Fisheries and Mariculture Laboratory and filtering the seawater through sieves of three different mesh sizes to obtain three size fractions of plankton samples (10-60 µm, 100-200 µm, and 200-500 µm). Bottom sediment was collected using a grab sampler and passed through a 60‑µm sieve to obtain benthic particulate organic matter. Samples of jellyfish and small fishes were taken with dip nets, cast nets, or seines and processed as described above for analysis.</p>
<p>Fatty acid profiles&nbsp;were measured for samples of fish eggs, marine animals, and basal resources collected from the field as well as eggs and marine animals used in laboratory experiments. The time between collection and analysis ranged from 2 months to a year.</p>
<p>For fatty acid analysis, each sample was lyophilized, homogenized, and weighed. Lipids were cold extracted from a known subsample (by weight) of the homogenized and lyophilized tissue using chloroform/methanol/water (8:4:3 v/v) following the method of Folch et al. (1957. Journal of Biological Chemistry 226:497–507) with 0.01% (w/v) butylated hydroxytoluene as an antioxidant. For small taxa or taxa with low lipid content (e.g., ctenophores), several individuals were pooled into one sample. Fatty acid methyl esters (FAME) were prepared by transesterification with 14% boron trifluoride in methanol (Morrison and Smith 1964. Journal of Lipid Research 5:600–608) following saponification of the total lipid using 0.5M potassium hydroxide in methanol. FAMEs were measured by gas chromatography with flame ionization detector following procedures described by Faulk and Holt (2005. Aquaculture 249:231–243). Measurements were expressed in terms of concentration (mg per g dry weight) and composition (% of total fatty acids). Fatty acids were identified by comparison with commercial standards.</p>
<p>Known Issues:<br />
Quality control procedure&nbsp;&nbsp;<br />
Principal components analysis for each taxon was performed on the mg g-1 dw and percent total fatty acids data, separately.&nbsp;&nbsp; Individual samples for which the score on the first or second principal component axis were greater than 4 standard deviations from the taxon mean were removed from the data set.&nbsp;</p>
<p>A primary check value was assigned as follows:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Perfectly fine</p>
<p>2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Data not evaluated because of too few data points for principal components analysis</p>
<p>The quantification limit for individual fatty acids is 0.013 mg g-1 dry weight</p>
<p>Measured values of any fatty acid less than 0.013 mg g-1 dw were changed to 0.000 mg g-1 dw and its corresponding value for percentage of total fatty acids was changed to 0.0%.</p>
<p>FAML: pier at in Corpus Christi Channel, Port Aransas, TX, United States, Fisheries and Mariculture Laboratory of the University of Texas Marine Science Institute (lat. 27.8396111, lon. -97.0727222);&nbsp;</p>
<p>MI: Mud Island in Aransas Bay, TX, United States (lat. 27.9362222, lon. -97.0217777)).</p>
Specified by the Principal Investigator(s)
<p><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">Identified peaks from the gas chromatograms were quantified using commercial FAME standards, such as Supelco 37 Component FAME mix, polyunsaturated fatty acid mix no. 3 (PUFA-3), and bacterial acid methyl ester mix (BAME).</span></p>
Specified by the Principal Investigator(s)
BCO-DMO Data Manager Processing Notes:
* Sheet 1 of file "Field FA data 2020-2022.xlsx" (submitted jun 22, 2023 by email) was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.
* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]
* Dates converted to ISO 8601 format
* one instance of FAMl with lowercase l changed to FAML for consistency with the rest of the dataset and related datasets.
* lat lon columns added from locations in metadata
* updated longitude for site TPWD (update provided by submitter) lon. -97.3150222
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
Shimadzu GC-2014 gas chromatograph with a flame ionization detector
PI Supplied Instrument Name: Shimadzu GC-2014 gas chromatograph with a flame ionization detector Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/