http://lod.bco-dmo.org/id/dataset/910948
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-10-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Concentrations of particulate organic matter (POC, PON, POP, PCOD), total oxygen demand, POM ratios, and respiration ratios from samples collected on R/V Atlantic Explorer BATS validation study #58 in the Sargasso Sea in October 2021
2023-10-10
publication
2023-10-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-10-10
publication
https://doi.org/10.26008/1912/bco-dmo.910948.1
Adam Martiny
University of California-Irvine
principalInvestigator
Rodney J. Johnson
Bermuda Institute of Ocean Sciences
principalInvestigator
Paul J. Lethaby
Bermuda Institute of Ocean Sciences
principalInvestigator
Allison R. Moreno
University of California-Los Angeles
principalInvestigator
Francois Primeau
University of California-Irvine
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gerace, S. D., Fagan, A. J., Primeau, F., Moreno, A. R., Lethaby, P. J., Johnson, R. J., Martiny, A. (2023) Concentrations of particulate organic matter (POC, PON, POP, PCOD), total oxygen demand, POM ratios, and respiration ratios from samples collected on R/V Atlantic Explorer BATS validation study #58 in the Sargasso Sea in October 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-10-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.910948.1 [access date]
Particulate Organic Matter from BVal58 Cruise Dataset Description: Methods and Sampling: <p>Samples were collected on the&amp; R/V Atlantic Explorer BATS Validation cruise #58 (BVal58) (cruise ID AE2121), which took place from October 11 to 25, 2021, and transited from St. George's, Bermuda to San Juan, Puerto Rico. The research group collecting these data disembarked on October 21, 2021.</p>
<p><strong>Suspended POM Sampling in the Euphotic Zone (Depths of 5 - 120 meters):</strong><br />
Seawater was collected at 5, 40, 80, and 120 meters (m) deep using a CTD rosette fitted with twenty-four 10-liter (L) Niskin bottles (OTE). All sampling carboys were rinsed 3 times with collected seawater before filling to 8 L. Particulate Organic Matter (POM) was collected on 25-millimeter (mm) GF/F filters (0.7-micrometer (µm) pore size, Whatman, GE Healthcare) pre-combusted at 500° Celsius (C) for 4 hours. The 6 filters collected at each depth of each station resulted in duplicate samples for the Particulate Organic Carbon and Nitrogen (POC/N), Particulate Organic Phosphorus (POP), and Particulate Chemical Oxygen Demand (PCOD) assays. After filtering 8 L, the filters for the POP assay were rinsed with 5 milliliters (mL) of 0.17 molar (M) NaSO₄ to remove dissolved inorganic phosphorus, and the filters for the PCOD assay were rinsed with 5 mL of deionized water to remove chloride ions. All filters were folded in half after filtration, sealed inside pre-combusted aluminum foil (500°C for 4 hours), and stored at -80°C. Post-assays, we accounted for POM on blank filters by subtracting the average value of 10 dry blanks. The dry blanks were pre-combusted filters that had not been used for filtering.</p>
<p><strong>Suspended POM Sampling in the Disphotic Zone (Depths of 150 - 1000 m):</strong><br />
McLane WTS-LV pumps were lowered to depths of 150, 200, 300, 400, 500, and 1000 m and each directly filtered 557 to 821 L of seawater through a 142 mm diameter pre-combusted GF/F filter (0.7 µm pore size, Whatman, GE Healthcare). After recovering the McLane pumps, the filters were similarly folded in half, sealed with pre-combusted aluminum foil, and stored at -80°C. Post-cruise, we hole-punched the 142 mm diameter filters in 15 places with a carbon-steel 18 mm hole-puncher that had been pre-combusted (500°C for 4 hours). Taking into account the O-ring, each hole punch equaled 3.8% of the total filter area containing POM. One to four chad pairs served as one replicate sample depending on the sensitivity of the assay. Two to four replicate samples were made for each assay. Dry blanks were prepared by hole-punching a pre-combusted, but unused 142 mm GF/F filter.</p>
<p><strong>Particulate Organic Carbon and Nitrogen (POC/N) Assay:</strong><br />
POC/N filters were processed using a JGOFS protocol (Ducklow &amp; Dickson, 1994). Filters for the POC/N assay were dried at 55°C for 24 hours. Filters were then placed in a desiccator with 12 M hydrochloric acid for 24 hours to remove inorganic carbonates. Filters were then re-dried for a minimum of 48 hours at 55°C. After drying, the filters were folded and pelletized into pre-combusted tin capsules (CE Elantech, Lakewood, NJ). Each tin-wrapped sample was analyzed in a FlashEA 1112 Elemental Analyzer using the NC Soils setup (Thermo Scientific, Waltham, MA). Known masses of atropine and acetanilide were used as standards for each run. The minimum detection limits for carbon and nitrogen were 2.4 micrograms (µg) and 3.0 µg respectively.</p>
<p><strong>Particulate Organic Phosphorus (POP) Assay:</strong><br />
We quantified particulate organic phosphorus using an ash-hydrolysis method presented by Lomas et al. (2010). Filters were placed in autoclaved glass vials with 2 mL of 0.017 M MgSO₄, covered with pre-combusted aluminum foil (500°C for 4 hours), and then combusted at 500°C for 2 hours. 5 mL 0.2 M HCl was added and incubated at 80° to 90°C for 30 minutes. After cooling, the solution was poured into a glass centrifuge tube. The glass vial was rinsed with 5 mL of deionized water, which was then poured into the same centrifuge tube. A mixed reagent of 0.0243 M ammonium molybdate tetrahydrate, 5 N sulfuric acid, 0.004 M potassium antimonyl tartrate, and 0.3 M ascorbic acid (2:5:1:2) was added to each tube before being set in the dark for 30 minutes. Tubes were then centrifuged at 4000 rotations per minute (rpm) and quantified at 885 nanometers (nm) with a spectrophotometer using a potassium monobasic phosphate standard (1.0 mM-P). The minimum detection limit was 0.3 µg for phosphorus.</p>
<p><strong>Particulate Chemical Oxygen Demand (PCOD) Assay:</strong><br />
The PCOD assay is a wastewater assay that has been modified by Moreno et al. (2020) to accurately quantify oxygen needed to fully oxidize organic carbon on GF/F filters. Note that because dichromate does not oxidize ammonium, this assay does not quantify oxygen demand for nitrification. Prior to the assay, the filters were dried at 55°C for at least 24 hours. For analysis, filters were added into HACH COD HR+ reagent vials (Product no. 2415915 containing mercuric sulfate) with 2 mL of milli-Q water. Vials were digested at 150°C for 2 hours, then 92.1 μL of 0.163 M NaCl was added to induce even precipitation of silver chloride. Vials were then inverted twice and centrifuged for 30 minutes at 2500 rpm. The absorbance of each vial was measured at 600 nm using a spectrophotometer. The oxygen demand was quantified using a standard curve made from HACH certified phthalate-based standards. The minimum detection limit was 3.1 µg for oxygen demand.</p>
<p><strong>Calculating Ratios and Σ-O₂</strong>:<br />
Elemental ratios (micromolar:micromolar (µM:µM)) were quantified using mean concentrations from the same depth and station. We quantified the total oxygen demand for complete remineralization (Σ-O₂) as PCOD plus double PON in units of micromolar.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1948842 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1948842
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Rodney J. Johnson
Bermuda Institute of Ocean Sciences
441-297-1880
7 Biological Station
St. Georges
GE 01
Bermuda
rod.johnson@bios.edu
pointOfContact
Paul J. Lethaby
Bermuda Institute of Ocean Sciences
Bermuda
paul.lethaby@bios.edu
pointOfContact
Allison R. Moreno
University of California-Los Angeles
UCLA Atmospheric & Oceanic Sciences
Los Angeles
CA
USA
allimoreno@atmos.ucla.edu
pointOfContact
Francois Primeau
University of California-Irvine
949-824-9435
Earth System Science & Ecology and Evolutionary Biology
Irvine
CA
92697
USA
fprimeau@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
McLane_Cast
ISO_DateTime_UTC
Latitude
Longitude
Depth_m
Volume_L
Filter_Num
Duplicate_Filter_Num
POP1
POP2
POP3
POP4
POP5
POP6
POP_Avg_nM
POP_Avg_microM
POC1
POC2
POC3
POC4
POC_Avg_microM
PON1
PON2
PON3
PON4
PON_Avg_mircoM
PCOD1
PCOD2
PCOD3
PCOD4
PCOD5
PCOD_Avg_microM
C_N
C_P
N_P
PCOD_POC
Sigma_O2_C
Sigma_O2_N
Sigma_O2_P
Sea-bird 911 CTD fitted with twenty-four 10 Niskin (OTE) bottles
Thermo Scientific FlashEA 1112
McLane Large Volume - Water Transfer System (WTS-LV) pumps
10 L Niskin bottles (OTE)
theme
None, User defined
station
cast
ISO_DateTime_UTC
latitude
longitude
depth
volume
sample identification
particulate organic phosphorus
particulate organic Carbon (POC)
particulate organic nitrogen
PCOD
ratio
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
Elemental Analyzer
McLane Large Volume Pumping System WTS-LV
Niskin bottle
instrument
BCO-DMO Standard Instruments
AE2121
service
Deployment Activity
Subtropical North Atlantic (Sargasso Sea). From St. George's, Bermuda to San Juan, Puerto Rico.
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Quantifying ocean oxygen-to-carbon demand by chemical analyses and inverse models
https://osprey.bco-dmo.org/project/908982
Quantifying ocean oxygen-to-carbon demand by chemical analyses and inverse models
<p><em>NSF Award Abstract:</em><br />
The marine carbon and oxygen cycles are intimately linked through the production and consumption of oxygen during photosynthesis and respiration. The amount of oxygen consumed per mole of respired organic carbon, the respiration quotient, is key for accurately predicting ocean oxygen concentrations and how ocean oxygen levels will respond to climate change. Despite this importance, the respiration quotient has rarely been measured directly. The aim of this research is to estimate the respiration quotient and its variability across ocean regions and depth. This will be done through an integration of direct chemical measurements of particulate organic matter collected from multiple ocean regions and indirectly by using an ocean circulation model to analyze a global database of dissolved oxygen and carbon concentration with a circulation model. In addition to advancing the understanding of the ocean oxygen cycle in the scientific community, this project will provide research training for researchers and students. The project will also improve awareness and understanding in the general public about possible future changes to marine oxygen concentrations.</p>
<p>One of the main uncertainties in predicting current and future oxygen levels is the regulation of the biological respiration demand. The respiration quotient describes the amount of oxygen needed during the consumption of one mole of organic carbon and thus is a key link between the carbon and oxygen cycles. Here, we propose to measure the amount of oxygen consumed per mole of respired organic carbon, the respiration quotient, in particulate organic matter (POM) from the surface and thermocline using a newly developed chemical technique. Thus, we aim to obtain new direct chemical measurements of the respiration quotient across distinct oceanic regimes and depths. Subsequently, we propose to incorporate these observations into a seasonally-resolved inverse model using global hydrographic tracer concentration data. This combined approach will provide the first global-scale estimates (and uncertainties) of the mean and regional variability of the respiration quotient in the POM stock and export flux. Different parameterizations linking the respiration quotient to regions or environmental variables will be applied to test how they impact and replicate global oxygen concentrations and the extent of the oxygen minimum zones.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
oxygen-to-carbon demand
largerWorkCitation
project
eng; USA
oceans
Subtropical North Atlantic (Sargasso Sea). From St. George's, Bermuda to San Juan, Puerto Rico.
-65.967
-64.168
19.667
31.667
2021-10-11
2021-10-19
Subtropical North Atlantic (Sargasso Sea) from St. George's, Bermuda to San Juan, Puerto Rico
0
BCO-DMO catalogue of parameters from Concentrations of particulate organic matter (POC, PON, POP, PCOD), total oxygen demand, POM ratios, and respiration ratios from samples collected on R/V Atlantic Explorer BATS validation study #58 in the Sargasso Sea in October 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/911029.rdf
Name: Station
Units: unitless
Description: <p>station number where sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/911030.rdf
Name: McLane_Cast
Units: unitless
Description: <p>cast number for McLane pump deployments</p>
http://lod.bco-dmo.org/id/dataset-parameter/911031.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>date and time (UTC) of sample collection in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/911032.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/911033.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude; negative values = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/911034.rdf
Name: Depth_m
Units: meters (m)
Description: <p>depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/911035.rdf
Name: Volume_L
Units: liters (L)
Description: <p>sample volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/911036.rdf
Name: Filter_Num
Units: unitless
Description: <p>number for GF/F used</p>
http://lod.bco-dmo.org/id/dataset-parameter/911037.rdf
Name: Duplicate_Filter_Num
Units: unitless
Description: <p>number for GF/F used if a duplicate filter was used</p>
http://lod.bco-dmo.org/id/dataset-parameter/911038.rdf
Name: POP1
Units: nanomolar (nM)
Description: <p>POP sample replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/911039.rdf
Name: POP2
Units: nanomolar (nM)
Description: <p>POP sample replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/911040.rdf
Name: POP3
Units: nanomolar (nM)
Description: <p>POP sample replicate #3</p>
http://lod.bco-dmo.org/id/dataset-parameter/911041.rdf
Name: POP4
Units: nanomolar (nM)
Description: <p>POP sample replicate #4</p>
http://lod.bco-dmo.org/id/dataset-parameter/911042.rdf
Name: POP5
Units: nanomolar (nM)
Description: <p>POP sample replicate #5</p>
http://lod.bco-dmo.org/id/dataset-parameter/911043.rdf
Name: POP6
Units: nanomolar (nM)
Description: <p>POP sample replicate #6</p>
http://lod.bco-dmo.org/id/dataset-parameter/911044.rdf
Name: POP_Avg_nM
Units: nanomolar (nM)
Description: <p>average of POP sample replicates in nanomolar</p>
http://lod.bco-dmo.org/id/dataset-parameter/911045.rdf
Name: POP_Avg_microM
Units: micromolar (uM)
Description: <p>average of POP sample replicates in micromolar</p>
http://lod.bco-dmo.org/id/dataset-parameter/911046.rdf
Name: POC1
Units: micromolar (uM)
Description: <p>POC sample replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/911047.rdf
Name: POC2
Units: micromolar (uM)
Description: <p>POC sample replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/911048.rdf
Name: POC3
Units: micromolar (uM)
Description: <p>POC sample replicate #3</p>
http://lod.bco-dmo.org/id/dataset-parameter/911049.rdf
Name: POC4
Units: micromolar (uM)
Description: <p>POC sample replicate #4</p>
http://lod.bco-dmo.org/id/dataset-parameter/911050.rdf
Name: POC_Avg_microM
Units: micromolar (uM)
Description: <p>average of POC sample replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/911051.rdf
Name: PON1
Units: micromolar (uM)
Description: <p>PON sample replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/911052.rdf
Name: PON2
Units: micromolar (uM)
Description: <p>PON sample replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/911053.rdf
Name: PON3
Units: micromolar (uM)
Description: <p>PON sample replicate #3</p>
http://lod.bco-dmo.org/id/dataset-parameter/911054.rdf
Name: PON4
Units: micromolar (uM)
Description: <p>PON sample replicate #4</p>
http://lod.bco-dmo.org/id/dataset-parameter/911055.rdf
Name: PON_Avg_mircoM
Units: micromolar (uM)
Description: <p>average of PON sample replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/911056.rdf
Name: PCOD1
Units: micromolar (uM)
Description: <p>PCOD sample replicate #1</p>
http://lod.bco-dmo.org/id/dataset-parameter/911057.rdf
Name: PCOD2
Units: micromolar (uM)
Description: <p>PCOD sample replicate #2</p>
http://lod.bco-dmo.org/id/dataset-parameter/911058.rdf
Name: PCOD3
Units: micromolar (uM)
Description: <p>PCOD sample replicate #3</p>
http://lod.bco-dmo.org/id/dataset-parameter/911059.rdf
Name: PCOD4
Units: micromolar (uM)
Description: <p>PCOD sample replicate #4</p>
http://lod.bco-dmo.org/id/dataset-parameter/911060.rdf
Name: PCOD5
Units: micromolar (uM)
Description: <p>PCOD sample replicate #5</p>
http://lod.bco-dmo.org/id/dataset-parameter/911061.rdf
Name: PCOD_Avg_microM
Units: micromolar (uM)
Description: <p>average of PCOD sample replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/911062.rdf
Name: C_N
Units: unitless (ratio of uM:uM)
Description: <p>average POC/PON for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911063.rdf
Name: C_P
Units: unitless (ratio of uM:uM)
Description: <p>average POC/POP for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911064.rdf
Name: N_P
Units: unitless (ratio of uM:uM)
Description: <p>average PON/POP for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911065.rdf
Name: PCOD_POC
Units: unitless (ratio of uM:uM)
Description: <p>average respiration quotient, PCOD/POC, for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911066.rdf
Name: Sigma_O2_C
Units: unitless (ratio of uM:uM)
Description: <p>average total respiraiton quotient, (PCOD + 2PON) /POC, for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911067.rdf
Name: Sigma_O2_N
Units: unitless (ratio of uM:uM)
Description: <p>average (PCOD + 2PON)/PON for depth and station</p>
http://lod.bco-dmo.org/id/dataset-parameter/911068.rdf
Name: Sigma_O2_P
Units: unitless (ratio of uM:uM)
Description: <p>average (PCOD + 2PON)/POP for depth and station</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
16197
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/19e5eada-12a7-4194-b82c-00037f482721/content
download
https://doi.org/10.26008/1912/bco-dmo.910948.1
download
onLine
dataset
<p>Samples were collected on the&amp; R/V Atlantic Explorer BATS Validation cruise #58 (BVal58) (cruise ID AE2121), which took place from October 11 to 25, 2021, and transited from St. George's, Bermuda to San Juan, Puerto Rico. The research group collecting these data disembarked on October 21, 2021.</p>
<p><strong>Suspended POM Sampling in the Euphotic Zone (Depths of 5 - 120 meters):</strong><br />
Seawater was collected at 5, 40, 80, and 120 meters (m) deep using a CTD rosette fitted with twenty-four 10-liter (L) Niskin bottles (OTE). All sampling carboys were rinsed 3 times with collected seawater before filling to 8 L. Particulate Organic Matter (POM) was collected on 25-millimeter (mm) GF/F filters (0.7-micrometer (µm) pore size, Whatman, GE Healthcare) pre-combusted at 500° Celsius (C) for 4 hours. The 6 filters collected at each depth of each station resulted in duplicate samples for the Particulate Organic Carbon and Nitrogen (POC/N), Particulate Organic Phosphorus (POP), and Particulate Chemical Oxygen Demand (PCOD) assays. After filtering 8 L, the filters for the POP assay were rinsed with 5 milliliters (mL) of 0.17 molar (M) NaSO₄ to remove dissolved inorganic phosphorus, and the filters for the PCOD assay were rinsed with 5 mL of deionized water to remove chloride ions. All filters were folded in half after filtration, sealed inside pre-combusted aluminum foil (500°C for 4 hours), and stored at -80°C. Post-assays, we accounted for POM on blank filters by subtracting the average value of 10 dry blanks. The dry blanks were pre-combusted filters that had not been used for filtering.</p>
<p><strong>Suspended POM Sampling in the Disphotic Zone (Depths of 150 - 1000 m):</strong><br />
McLane WTS-LV pumps were lowered to depths of 150, 200, 300, 400, 500, and 1000 m and each directly filtered 557 to 821 L of seawater through a 142 mm diameter pre-combusted GF/F filter (0.7 µm pore size, Whatman, GE Healthcare). After recovering the McLane pumps, the filters were similarly folded in half, sealed with pre-combusted aluminum foil, and stored at -80°C. Post-cruise, we hole-punched the 142 mm diameter filters in 15 places with a carbon-steel 18 mm hole-puncher that had been pre-combusted (500°C for 4 hours). Taking into account the O-ring, each hole punch equaled 3.8% of the total filter area containing POM. One to four chad pairs served as one replicate sample depending on the sensitivity of the assay. Two to four replicate samples were made for each assay. Dry blanks were prepared by hole-punching a pre-combusted, but unused 142 mm GF/F filter.</p>
<p><strong>Particulate Organic Carbon and Nitrogen (POC/N) Assay:</strong><br />
POC/N filters were processed using a JGOFS protocol (Ducklow &amp; Dickson, 1994). Filters for the POC/N assay were dried at 55°C for 24 hours. Filters were then placed in a desiccator with 12 M hydrochloric acid for 24 hours to remove inorganic carbonates. Filters were then re-dried for a minimum of 48 hours at 55°C. After drying, the filters were folded and pelletized into pre-combusted tin capsules (CE Elantech, Lakewood, NJ). Each tin-wrapped sample was analyzed in a FlashEA 1112 Elemental Analyzer using the NC Soils setup (Thermo Scientific, Waltham, MA). Known masses of atropine and acetanilide were used as standards for each run. The minimum detection limits for carbon and nitrogen were 2.4 micrograms (µg) and 3.0 µg respectively.</p>
<p><strong>Particulate Organic Phosphorus (POP) Assay:</strong><br />
We quantified particulate organic phosphorus using an ash-hydrolysis method presented by Lomas et al. (2010). Filters were placed in autoclaved glass vials with 2 mL of 0.017 M MgSO₄, covered with pre-combusted aluminum foil (500°C for 4 hours), and then combusted at 500°C for 2 hours. 5 mL 0.2 M HCl was added and incubated at 80° to 90°C for 30 minutes. After cooling, the solution was poured into a glass centrifuge tube. The glass vial was rinsed with 5 mL of deionized water, which was then poured into the same centrifuge tube. A mixed reagent of 0.0243 M ammonium molybdate tetrahydrate, 5 N sulfuric acid, 0.004 M potassium antimonyl tartrate, and 0.3 M ascorbic acid (2:5:1:2) was added to each tube before being set in the dark for 30 minutes. Tubes were then centrifuged at 4000 rotations per minute (rpm) and quantified at 885 nanometers (nm) with a spectrophotometer using a potassium monobasic phosphate standard (1.0 mM-P). The minimum detection limit was 0.3 µg for phosphorus.</p>
<p><strong>Particulate Chemical Oxygen Demand (PCOD) Assay:</strong><br />
The PCOD assay is a wastewater assay that has been modified by Moreno et al. (2020) to accurately quantify oxygen needed to fully oxidize organic carbon on GF/F filters. Note that because dichromate does not oxidize ammonium, this assay does not quantify oxygen demand for nitrification. Prior to the assay, the filters were dried at 55°C for at least 24 hours. For analysis, filters were added into HACH COD HR+ reagent vials (Product no. 2415915 containing mercuric sulfate) with 2 mL of milli-Q water. Vials were digested at 150°C for 2 hours, then 92.1 μL of 0.163 M NaCl was added to induce even precipitation of silver chloride. Vials were then inverted twice and centrifuged for 30 minutes at 2500 rpm. The absorbance of each vial was measured at 600 nm using a spectrophotometer. The oxygen demand was quantified using a standard curve made from HACH certified phthalate-based standards. The minimum detection limit was 3.1 µg for oxygen demand.</p>
<p><strong>Calculating Ratios and Σ-O₂</strong>:<br />
Elemental ratios (micromolar:micromolar (µM:µM)) were quantified using mean concentrations from the same depth and station. We quantified the total oxygen demand for complete remineralization (Σ-O₂) as PCOD plus double PON in units of micromolar.</p>
Specified by the Principal Investigator(s)
<p>Data were processed using MATLAB R2021b.</p>
<p>Any gaps in sampling are marked as missing entries in the dataset.</p>
Specified by the Principal Investigator(s)
- Imported original file named "BVal58 POM Data Set S1.xlsx" into the BCO-DMO system.
- Renamed parameters/columns to comply with BCO-DMO naming conventions.
- Created ISO 8601 date/time (UTC) column by combining year, month, day, and time fields.
- Removed the original year, month, day, and time columns.
- Saved the final file as "910948_v1_bval58_pom.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sea-bird 911 CTD fitted with twenty-four 10 Niskin (OTE) bottles
Sea-bird 911 CTD fitted with twenty-four 10 Niskin (OTE) bottles
PI Supplied Instrument Name: Sea-bird 911 CTD fitted with twenty-four 10 Niskin (OTE) bottles Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
Thermo Scientific FlashEA 1112
Thermo Scientific FlashEA 1112
PI Supplied Instrument Name: Thermo Scientific FlashEA 1112 Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
McLane Large Volume - Water Transfer System (WTS-LV) pumps
McLane Large Volume - Water Transfer System (WTS-LV) pumps
PI Supplied Instrument Name: McLane Large Volume - Water Transfer System (WTS-LV) pumps Instrument Name: McLane Large Volume Pumping System WTS-LV Instrument Short Name:WTS-LV Instrument Description: The WTS-LV is a Water Transfer System (WTS) Large Volume (LV) pumping instrument designed and manufactured by McLane Research Labs (Falmouth, MA, USA). It is a large-volume, single-event sampler that collects suspended and dissolved particulate samples in situ.
Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.
The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.
10 L Niskin bottles (OTE)
10 L Niskin bottles (OTE)
PI Supplied Instrument Name: 10 L Niskin bottles (OTE) Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AE2121
AE2121
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE2121
Paul J. Lethaby
Bermuda Institute of Ocean Sciences
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel