http://lod.bco-dmo.org/id/dataset/913222
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2023-10-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Photosynthetic pigments from sea ice samples collected on R/V Nathaniel B. Palmer cruise NBP1910 along the Western Antarctic Peninsula from November to December 2019
2023-10-17
publication
2023-10-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-10-26
publication
https://doi.org/10.26008/1912/bco-dmo.913222.1
Jodi N. Young
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Young, J. N. (2023) Photosynthetic pigments from sea ice samples collected on R/V Nathaniel B. Palmer cruise NBP1910 along the Western Antarctic Peninsula from November to December 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-10-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.913222.1 [access date]
Photosynthetic Pigments Dataset Description: Methods and Sampling: <p><strong>Field sampling:</strong><br />
Ice samples for primary production measurements were collected mid-morning from 6 stations along the western Antarctic Peninsula in November and December of 2019 on board the R/V Nathaniel B. Palmer along a north-south transect from 64.8°S to 67.8°S. For Stations (Stns) 2 and 3, the ice was "rotten" (sufficiently melted to be disintegrating structurally, present only in small pieces) and collected as an ice-seawater slurry. Small ice chunks were collected directly from the sea surface via a crane-suspended "personnel basket". At the additional 4 stations, sea ice was collected by coring the ice. At Stns 4 and 7, algal samples were collected from internal ice-core layers. Stns 4 and 7 were rafted floes with a flooded internal layer, with Stn 7 &gt; 2500 square meters (m²) in size. Stns 5 and 6 were on landfast sea ice, where the algae were collected from the bottom 10 centimeters (cm) of the ice. At these 4 stations (Stns 4 through 7), ice cores were taken with a 7.5 cm Kovacs corer separated by at least 1 m horizontally. Cores were shaded from direct sunlight, while 5 to 10 cm of the visible algal band at the bottom or middle was sectioned with an ethanol-cleaned saw and placed into acid-washed (10% HCl) containers. Each replicate consisted of either a single core (for 10 cm sections) or pools of two cores (for 5 cm sections).</p>
<p>All ice samples were stored in dark, insulated containers for a maximum of 4 hours. On the ship, all ice samples used for primary production measurements were melted in a 3:1 volumetric ratio of melting solution to ice to minimize the effect of the melt on sea-ice communities. The melting solution was a 0.2 micrometer (μm) filtered artificial salt mixture containing three main sea salts plus bicarbonate according to ESAW artificial seawater (3.63 x 10⁻¹ molar (M) NaCl, 4.71 x 10⁻² M MgCl₂.6H₂O, 2.5 x 10⁻² M Na₂SO₄, and 2 x 10⁻³ M NaHCO₃⁻, salinity 35). Additional ice cores collected for ancillary biological measurements were melted in a 1:1 volumetric ratio of melting solution to ice. Melts were conducted in the dark at approximately 20° Celsius (C). To speed the melting process, ice samples were further broken into pieces with acid-washed pickaxes with most ice completely melted within 5 hours. Volume and salinity were measured as soon as the ice was completely melted, with sample temperatures remaining below 0°C. All reported volumes were corrected to the original ice volume.</p>
<p><strong>Pigments:</strong><br />
A subsample of 1:1 melt volume was filtered on 25-millimeter (mm) GF/Fs for pigment analysis by high-performance liquid chromatography (HPLC). Filters were promptly flash-frozen in liquid nitrogen and stored at -80°C until analysis. Reverse-phase high-pressure liquid chromatography was conducted at the University of South Carolina after the method detailed by Pinckney et al. (1998). To estimate the relative abundance of diatoms, <em>Phaeocystis</em>, and cryptophytes (as contribution to Chl <em>a</em> in milligrams per liter (mg L⁻¹)), the respective diagnostic pigments of fucoxanthin, 19' hexanoyloxyfucoxanthin, and alloxanthin were used as in Everitt et al. (1990) and Arrigo et al. (2000). Pigment data was qualitatively confirmed via light microscopy.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1744645 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1744645
completed
Jodi N. Young
University of Washington
206-543-0744
School of Oceanography Box 357940
Seattle
WA
98195
USA
youngjn@uw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Latitude
Longitude
Description
Sample_id
Replicate
Chl_c3
Chl_c1c2
Perid
ButFuc19
Fuco
HexFuc19
Neo
Prasino
Viola
Diad
Anther
Allox
Diat
Lutein
Zeax
Gyro
Chl_b
Chla_Allomer
Chl_a
Chla_prime
alpha_Carotene
beta_Carotene
Chl_ide_a
Total_Chl_a
ALL_Chl_a
light microscopy
theme
None, User defined
date
latitude
longitude
sample description
sample identification
replicate
chlorophyll c3
chl_c1_c2
peridinin
19-prime-butanoyloxyfucoxanthin
fucoxanthin
19-prime-hexanoyloxyfucoxanthin
neoxanthin
prasinoxanthin
violaxanthin
diadinoxanthin
antherax
alloxanthin
diatoxanthin
lutein
zeaxanthin
Gyroxanthin-Diester
chlorophyll b
allomerized chlorophyll a
chlorophyll a
chlorophyll a prime
carotene-alpha
carotene-beta
chlorophyllide a
chl_tot
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
Ice Corer
Microscope - Optical
instrument
BCO-DMO Standard Instruments
NBP1910
service
Deployment Activity
Western Antarctic Peninsula
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Spring Blooms of Sea Ice Algae Along the Western Antarctic Peninsula: Effects of Warming and Freshening on Cell Physiology and Biogeochemical Cycles.
https://osprey.bco-dmo.org/project/908709
Spring Blooms of Sea Ice Algae Along the Western Antarctic Peninsula: Effects of Warming and Freshening on Cell Physiology and Biogeochemical Cycles.
<p><strong>NSF Award Abstract</strong><br />
Rapid changes in the extent and thickness of sea ice during the austral spring subject microorganisms within or attached to the ice to large fluctuations in temperature, salinity, light and nutrients. This project aims to identify cellular responses in sea-ice algae to increasing temperature and decreasing salinity during the spring melt along the western Antarctic Peninsula and to determine how associated changes at the cellular level can potentially affect dynamic, biologically driven processes. Understanding how sea-ice algae cope with, and are adapted to, their environment will not only help predict how polar ecosystems may change as the extent and thickness of sea ice change, but will also provide a better understanding of the widespread success of photosynthetic life on Earth. The scientific context and resulting advances from the research will be communicated to the general public through outreach activities that includes work with Science Communication Fellows and the popular Polar Science Weekend at the Pacific Science Center in Seattle, Washington. The project will provide student training to college students as well as provide for educational experiences for K-12 school children.</p>
<p>
There is currently a poor understanding of feedback relationships that exist between the rapidly changing environment in the western Antarctic Peninsula region and sea-ice algal production. The large shifts in temperature and salinity that algae experience during the spring melt affect critical cellular processes, including rates of enzyme-catalyzed reactions involved in photosynthesis and respiration, and the production of stress-protective compounds. These changes in cellular processes are poorly constrained but can be large and may have impacts on local ecosystem productivity and biogeochemical cycles. In particular, this study will focus on the thermal sensitivity of enzymes and the cycling of compatible solutes and exopolymers used for halo- and cryo-protection, and how they influence primary production and the biogeochemical cycling of carbon and nitrogen. Approaches will include field sampling during spring melt, incubation experiments of natural sea-ice communities under variable temperature and salinity conditions, and controlled manipulation of sea-ice algal species in laboratory culture. Employment of a range of techniques, from fast repetition rate fluorometry and gross and net photosynthetic measurements to metabolomics and enzyme kinetics, will tease apart the mechanistic effects of temperature and salinity on cell metabolism and primary production with the goal of quantifying how these changes will impact biogeochemical processes along the western Antarctic Peninsula.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Controls on Sea-Ice Algae (COSA)
largerWorkCitation
project
eng; USA
oceans
Western Antarctic Peninsula
-68.196
-64.02
-67.774
-64.78
2019-11-08
2019-12-05
Western Antarctic Peninsula
0
BCO-DMO catalogue of parameters from Photosynthetic pigments from sea ice samples collected on R/V Nathaniel B. Palmer cruise NBP1910 along the Western Antarctic Peninsula from November to December 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/913242.rdf
Name: Date
Units: unitless
Description: <p>date sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/913243.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude where sample was collected; negative values = South</p>
http://lod.bco-dmo.org/id/dataset-parameter/913244.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude where sample was collected; negative values = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/913245.rdf
Name: Description
Units: unitless
Description: <p>description of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/913246.rdf
Name: Sample_id
Units: unitless
Description: <p>unique identifier of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/913247.rdf
Name: Replicate
Units: unitless
Description: <p>unique replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/913248.rdf
Name: Chl_c3
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll c3</p>
http://lod.bco-dmo.org/id/dataset-parameter/913249.rdf
Name: Chl_c1c2
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll c1 and C2</p>
http://lod.bco-dmo.org/id/dataset-parameter/913250.rdf
Name: Perid
Units: micrograms per liter (ug/L)
Description: <p>concentration of peridinin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913251.rdf
Name: ButFuc19
Units: micrograms per liter (ug/L)
Description: <p>concentration of 19'-butanoyloxyfucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913252.rdf
Name: Fuco
Units: micrograms per liter (ug/L)
Description: <p>concentration of Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913253.rdf
Name: HexFuc19
Units: micrograms per liter (ug/L)
Description: <p>concentration of 19'-hexanoyloxyfucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913254.rdf
Name: Neo
Units: micrograms per liter (ug/L)
Description: <p>concentration of cis-neoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913255.rdf
Name: Prasino
Units: micrograms per liter (ug/L)
Description: <p>concentration of prasinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913256.rdf
Name: Viola
Units: micrograms per liter (ug/L)
Description: <p>concentration of violaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913257.rdf
Name: Diad
Units: micrograms per liter (ug/L)
Description: <p>concentration of diadinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913258.rdf
Name: Anther
Units: micrograms per liter (ug/L)
Description: <p>concentration of antheraxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913259.rdf
Name: Allox
Units: micrograms per liter (ug/L)
Description: <p>concentration of alloxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913260.rdf
Name: Diat
Units: micrograms per liter (ug/L)
Description: <p>concentration of diatoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913261.rdf
Name: Lutein
Units: micrograms per liter (ug/L)
Description: <p>concentration of Lutein</p>
http://lod.bco-dmo.org/id/dataset-parameter/913262.rdf
Name: Zeax
Units: micrograms per liter (ug/L)
Description: <p>concentration of Zeaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/913263.rdf
Name: Gyro
Units: micrograms per liter (ug/L)
Description: <p>concentration of gryoxanthin-diester</p>
http://lod.bco-dmo.org/id/dataset-parameter/913264.rdf
Name: Chl_b
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll b</p>
http://lod.bco-dmo.org/id/dataset-parameter/913265.rdf
Name: Chla_Allomer
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll a allomer</p>
http://lod.bco-dmo.org/id/dataset-parameter/913266.rdf
Name: Chl_a
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll a + divinyl chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/913267.rdf
Name: Chla_prime
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyll a'</p>
http://lod.bco-dmo.org/id/dataset-parameter/913268.rdf
Name: alpha_Carotene
Units: micrograms per liter (ug/L)
Description: <p>concentration of alpha Carotene</p>
http://lod.bco-dmo.org/id/dataset-parameter/913269.rdf
Name: beta_Carotene
Units: micrograms per liter (ug/L)
Description: <p>concentration of beta Carotene</p>
http://lod.bco-dmo.org/id/dataset-parameter/913270.rdf
Name: Chl_ide_a
Units: micrograms per liter (ug/L)
Description: <p>concentration of chlorophyllide a</p>
http://lod.bco-dmo.org/id/dataset-parameter/913271.rdf
Name: Total_Chl_a
Units: micrograms per liter (ug/L)
Description: <p>concentration of chl a + chl-ide a + divinyl chl a </p>
http://lod.bco-dmo.org/id/dataset-parameter/913272.rdf
Name: ALL_Chl_a
Units: micrograms per liter (ug/L)
Description: <p>concentration of chla + chla isomers</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4954
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/07b20292-a9e1-45ee-abba-628e6ca496d1/content
download
https://doi.org/10.26008/1912/bco-dmo.913222.1
download
onLine
dataset
<p><strong>Field sampling:</strong><br />
Ice samples for primary production measurements were collected mid-morning from 6 stations along the western Antarctic Peninsula in November and December of 2019 on board the R/V Nathaniel B. Palmer along a north-south transect from 64.8°S to 67.8°S. For Stations (Stns) 2 and 3, the ice was "rotten" (sufficiently melted to be disintegrating structurally, present only in small pieces) and collected as an ice-seawater slurry. Small ice chunks were collected directly from the sea surface via a crane-suspended "personnel basket". At the additional 4 stations, sea ice was collected by coring the ice. At Stns 4 and 7, algal samples were collected from internal ice-core layers. Stns 4 and 7 were rafted floes with a flooded internal layer, with Stn 7 &gt; 2500 square meters (m²) in size. Stns 5 and 6 were on landfast sea ice, where the algae were collected from the bottom 10 centimeters (cm) of the ice. At these 4 stations (Stns 4 through 7), ice cores were taken with a 7.5 cm Kovacs corer separated by at least 1 m horizontally. Cores were shaded from direct sunlight, while 5 to 10 cm of the visible algal band at the bottom or middle was sectioned with an ethanol-cleaned saw and placed into acid-washed (10% HCl) containers. Each replicate consisted of either a single core (for 10 cm sections) or pools of two cores (for 5 cm sections).</p>
<p>All ice samples were stored in dark, insulated containers for a maximum of 4 hours. On the ship, all ice samples used for primary production measurements were melted in a 3:1 volumetric ratio of melting solution to ice to minimize the effect of the melt on sea-ice communities. The melting solution was a 0.2 micrometer (μm) filtered artificial salt mixture containing three main sea salts plus bicarbonate according to ESAW artificial seawater (3.63 x 10⁻¹ molar (M) NaCl, 4.71 x 10⁻² M MgCl₂.6H₂O, 2.5 x 10⁻² M Na₂SO₄, and 2 x 10⁻³ M NaHCO₃⁻, salinity 35). Additional ice cores collected for ancillary biological measurements were melted in a 1:1 volumetric ratio of melting solution to ice. Melts were conducted in the dark at approximately 20° Celsius (C). To speed the melting process, ice samples were further broken into pieces with acid-washed pickaxes with most ice completely melted within 5 hours. Volume and salinity were measured as soon as the ice was completely melted, with sample temperatures remaining below 0°C. All reported volumes were corrected to the original ice volume.</p>
<p><strong>Pigments:</strong><br />
A subsample of 1:1 melt volume was filtered on 25-millimeter (mm) GF/Fs for pigment analysis by high-performance liquid chromatography (HPLC). Filters were promptly flash-frozen in liquid nitrogen and stored at -80°C until analysis. Reverse-phase high-pressure liquid chromatography was conducted at the University of South Carolina after the method detailed by Pinckney et al. (1998). To estimate the relative abundance of diatoms, <em>Phaeocystis</em>, and cryptophytes (as contribution to Chl <em>a</em> in milligrams per liter (mg L⁻¹)), the respective diagnostic pigments of fucoxanthin, 19' hexanoyloxyfucoxanthin, and alloxanthin were used as in Everitt et al. (1990) and Arrigo et al. (2000). Pigment data was qualitatively confirmed via light microscopy.</p>
Specified by the Principal Investigator(s)
<p>Analysis was carried out at the University of South Carolina Photopigment Analysis Facility. Details of instrumentation and protocols can be found at <a href="https://phytoninja.com/lab-protocols/" target="_blank">https://phytoninja.com/lab-protocols/</a> (see "HPLC Photopigment Analysis Method (technical version)") or in the attached PDF "Long_HPLC_Method.pdf".</p>
Specified by the Principal Investigator(s)
- Imported original file "phspigments.csv" into the BCO-DMO system.
- Converted the Date field to YYYY-MM-DD format.
- Split the Location column into two separate columns for Latitude and Longitude and made the values negative (for South and West directions).
- Renamed fields to comply with BCO-DMO naming conventions.
- Corrected a typo in the description column (replaced '0' with a closing parens ')' symbol in the last 5 rows).
- Saved the final file as "913222_v1_photosynthetic_pigments.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
PI Supplied Instrument Name: Instrument Name: Ice Corer Instrument Short Name:Ice Corer Instrument Description: An ice corer is used to drill into deep ice and remove long cylinders of ice from which information about the past and present can be inferred. Polar ice cores contain a record of the past atmosphere - temperature, precipitation, gas content, chemical composition, and other properties. This can reveal a broad spectrum of information on past environmental, and particularly climatic, changes. They can also be used to study bacteria and chlorophyll production in the waters from which the ice core was extracted. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/56/
light microscopy
light microscopy
PI Supplied Instrument Name: light microscopy Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Cruise: NBP1910
NBP1910
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel