http://lod.bco-dmo.org/id/dataset/914146 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2023-10-26 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Quantification of ciliated band length per unit protein in early echinoderm larvae (protein data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. 2024-04-10 publication 2024-04-10 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2024-04-10 publication https://doi.org/10.26008/1912/bco-dmo.914146.1 Bruno Pernet California State University Long Beach principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Steiner, B., Pernet, B. (2024) Quantification of ciliated band length per unit protein in early echinoderm larvae (protein data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-04-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.914146.1 [access date] Protein content of early echinoderm larvae Dataset Description: &lt;p&gt;&lt;strong&gt;Study Summary: &lt;/strong&gt;The feeding larvae of echinoderms take two distinct forms: plutei (echinoids, ophiuroids), which have calcified skeletal rods supporting long, slender arms bearing the ciliated band, and non-plutei (asteroids, holothuroids), where the ciliated band is borne on rounded lobes of tissue that do not contain skeletal rods. Feeding larvae of all four classes of echinoderms are known to alter the length of their ciliated bands in response to food ration, with larvae fed low rations producing longer ciliated bands relative to body size than larvae fed high rations. Prior work suggests that the structural cost of adding a given length of ciliated band might be lower for plutei than for non-plutei, which might affect the scope for phenotypic plasticity in ciliated band length in the two types of larvae.&lt;/p&gt; &lt;p&gt;In this study we test the hypothesis that plutei and support a greater length of ciliated band per unit biomass than non-plutei by comparing ciliated band length and protein content of larvae of eight species (with at least one species from each echinoderm class that includes feeding larvae) at two timepoints in early development.&lt;/p&gt; Methods and Sampling: &lt;p&gt;&lt;strong&gt;Collection of adults and spawning:&amp;nbsp; &lt;/strong&gt;Adults of each species were collected from intertidal or shallow subtidal zones from various sites in Los Angeles County and transported to California State University Long Beach, where they were maintained in recirculating seawater tanks at 16 °C until their use in experiments. Experiments were carried out on one species at a time, depending on reproductive seasonality for that species.&lt;/p&gt; &lt;p&gt;Spawning was induced using standard methods (e.g., M. Strathmann, 1987). The echinoids &lt;em&gt;Dendraster excentricus&lt;/em&gt;, &lt;em&gt;Lytechnius pictus&lt;/em&gt;, &lt;em&gt;Strongylocentrotus purpuratus&lt;/em&gt; and &lt;em&gt;S. fragilis&lt;/em&gt; were induced to spawn via injection of 0.2-1.0 mL (depending on adult size) 0.53 M KCL into the perivisceral coelom. The asteroids &lt;em&gt;Patiria miniata&lt;/em&gt; and &lt;em&gt;Astropecten armatus&lt;/em&gt; were induced to spawn by injection of 1-3 mL 100 µM 1-methyladenine. The holothuroid &lt;em&gt;Apostichopus parvimensis&lt;/em&gt; was injected with 3 mL of 200 µM NGLWY-amide (Kato et al., 2009). The ophiuroid &lt;em&gt;Ophiothrix spiculata&lt;/em&gt; was exposed to 4 °C water in the dark for 15 minutes, then to room temperature water and sunlight for 15 minutes; this treatment was repeated for up to two hours (Selvakumaraswamy &amp;amp; Byrne, 2000). For all species, adults were each induced to spawn in their own isolated containers, allowing us to control subsequent fertilizations. Spawning continued until a minimum of three parents of each sex were obtained. Sperm from each spawning male was combined with eggs from each spawning female. Once the larvae reached the swimming stage (~24 hours), the offspring of all parents were combined to produce a genetically diverse population.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Culturing: &lt;/strong&gt;Larvae were distributed into seven replicate 2 l beakers, with a total of 500 larvae per beaker (for &lt;em&gt;S. purpuratus&lt;/em&gt;, 14 beakers of 500 larvae each were produced since greater numbers were needed for their protein analysis due to their small size). Larvae were fed 6000 cells ml&lt;sup&gt;−1&lt;/sup&gt; &lt;em&gt;Rhodomonas lens&lt;/em&gt; which were isolated from their growth medium via centrifugation, resuspended in FSW, and counted using a BD Accuri C6 flow cytometer. Cultures were maintained in the 16 °C environmental chamber and continuously stirred by a paddle system (Strathmann, 1987). Daily water changes began on the third day post fertilization (dpf), allowing larvae to develop without disturbance for a day while still in or just completing the pre-feeding period. To change the water, cultures were filtered through a 60 µm sieve to capture larvae. The sieve was submerged in shallow water while filtering so larvae were not exposed to air. Larvae were then gently rinsed from the filter with fresh FSW back into their cleaned beakers and fed.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sampling:&lt;/strong&gt; For all species except &lt;em&gt;D. excentricus&lt;/em&gt;, sampling for images and protein analysis occurred at 5 (“early-development”) and 10 dpf (“mid-development”). Sampling for &lt;em&gt;D. excentricus&lt;/em&gt; occurred at 3 and 5 dpf due to their more rapid development and early formation of the rudiment. While larvae were concentrated during the water change, we collected larvae using a pulled pipette. Depending on larval size and age, three samples of 30-80 larvae each were collected from each beaker and placed in 1.5 ml microcentrifuge tubes on ice. The remaining larvae after the early-development sampling were returned to their beaker and FSW was added to approximate a larval concentration of 0.25 larvae ml&lt;sup&gt;−1&lt;/sup&gt; based on an estimation of remaining larvae. Both &lt;em&gt;Strongylocentrotus &lt;/em&gt;species introduced small variations to these methods, since their small size necessitated experiments with greater numbers. Thus, for &lt;em&gt;S. purpuratus&lt;/em&gt;, 14 replicate beakers were made on 1 dpf so three samples of 150 larvae each could be taken on 5 dpf from seven of the beakers (and the remaining larvae in those beakers discarded), and the other seven beakers were used for the mid-development samples of 80 larvae each. &lt;em&gt;S. fragilis &lt;/em&gt;was included in this study opportunistically, so we were not able to repeat the experiment with greater numbers and instead had fewer replicates for &lt;em&gt;S. fragilis &lt;/em&gt;than for the other species. &lt;em&gt;S. fragilis&lt;/em&gt; had a total of 6 replicate cultures instead of 7, and the 10 dpf timepoint consisted of only two protein samples rather than three due to a shortage of larvae.&lt;br /&gt; &lt;br /&gt; The tubes containing larvae sampled for protein analysis were centrifuged to pellet the larvae. Water was aspirated gently using a pipette pulled to a tip size of 0.2 mm. Once aspiration was complete, the samples were stored at -80 °C for up to three months.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Protein Quantification: &lt;/strong&gt;To process the protein samples, we added 100 µl of 4 °C Nanopure water to each sample, and placed them on ice. The larval cells were then lysed using Fisherbrand polypropylene pestles designed to fit 1.5 ml microcentrifuge tubes. A Thermo Scientific Micro BCA Protein Assay Kit was used to determine the protein content of samples against a BSA (Bovine Serum Albumin) standard curve. 376 µl of 4 °C Nanopure water to each to provide adequate volume for the assay. The samples were vortexed for 10-15 seconds. 150 µl from the homogenized sample was then placed into three experimental wells of a 96-well plate to produce three technical replicates per sample. After addition of reagents and incubation according to the manufacturer’s instructions, the plates were read using a Biotek Synergy H1 Hybrid Microplate Reader. To calculate protein content per individual, the number of larvae per technical replicate was calculated as: larvae in technical replicate = larvae in original sample *&amp;nbsp; (150 µl technical replicate volume)/(476 µl sample volume).&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756531 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756531 completed Bruno Pernet California State University Long Beach 562-985-5378 Biological Sciences, CSU Long Beach 1250 Bellflower Blvd. Long Beach CA 90840 USA bruno.pernet@csulb.edu pointOfContact asNeeded Dataset Version: 1 Unknown Species Age Dev Beaker Sample Average sd geospatial_bound_N geospatial_bound_S geospatial_bound_E geospatial_bound_W experiment_location_lat experiment_location_long experiment_start experiment_end BD Accuri C6 Flow Cytometer (BD Biosciences) Biotek Synergy H1 Hybrid Microplate Reader Thermo Scientific Micro BCA Protein Assay Kit theme None, User defined species age stage sample description sample identification mean standard deviation latitude longitude date featureType BCO-DMO Standard Parameters Flow Cytometer plate reader Protein Assay Kit instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. RUI: Effects of large inedible particles on larval feeding, planktonic larval duration, and juvenile quality in marine invertebrates https://osprey.bco-dmo.org/project/727167 RUI: Effects of large inedible particles on larval feeding, planktonic larval duration, and juvenile quality in marine invertebrates <p><em>NSF award abstract:</em><br /> Many ecologically and economically important marine invertebrates (e.g., oysters, crabs, and sea urchins) have life cycles that include feeding larval stages that live drifting in the water as part of the plankton. These larvae spend days or weeks feeding on tiny algal particles to fuel their development until they can metamorphose into juveniles. In nature, however, the plankton includes not only edible particles, but also many particles that are too large to be eaten but which may interfere with feeding on edible particles. These include, for example, large algal particles, eggs and embryos of other invertebrates, re-suspended sediment, and anthropogenic nano- and micro-plastics. When larvae encounter large inedible particles, they may respond by altering their swimming behavior to avoid them, or by capturing and then rejecting them. Such interactions reduce the rate at which larvae can capture edible particles, which forces them to either spend more time feeding before metamorphosis (increasing their overall risk of dying due to planktonic predators), or to metamorphose with less energy, producing juveniles in relatively poor condition. This project examines how large inedible particles affect feeding, time to metamorphosis, and juvenile condition in the larvae of diverse marine invertebrates. The project has the potential to dramatically change our understanding of how larvae feed and survive in natural communities, and thus our understanding of the population dynamics of these important organisms. The project will support research training opportunities for undergraduate and graduate students at California State University Long Beach, a primarily undergraduate institution, as well as summer research internships for students at two local community colleges. Project data will be integrated into laboratory modules in undergraduate courses. Finally, data on the reproductive biology of diverse California marine invertebrates will be added to a public website that is widely used by members of the public, students, and biologists interested in the development, life histories, ecology, and evolution of these common animals.</p> <p>The factors that control planktonic duration and juvenile condition in marine invertebrates with feeding larvae have long been recognized as critical to understanding their ecology and evolution. Larval feeding environment is clearly one of those factors, but previous work has focused almost exclusively on one feature of that environment, the abundance of food. This project will evaluate the importance of another potentially critical dimension of the larval feeding environment: the presence of large inedible particles, which are frequently abundant in natural plankonic communities. It takes a comparative approach to address two key questions about the effects of large inedible particles on larvae (including those of echinoderms, annelids, and molluscs) that feed using several different particle capture mechanisms. First, do large inedible particles present in natural plankton reduce larval feeding rates? And second, does the presence of large inedible particles extend larval planktonic duration or result in the production of lower quality juveniles? Feeding rates of larvae will be measured in short-term experiments in which larvae are exposed to both food and to natural or artificial large inedible particles over a range of concentrations. Effects of large inedible particles on planktonic duration and juvenile quality will be measured by culturing larvae through their entire life cycles in the presence of large inedible particles at various concentrations. Because feeding performance is an important determinant of planktonic duration, larval survival, and juvenile condition, the project will add greatly to our understanding of how conditions in the plankton affect the population dynamics of the many marine invertebrates with feeding larvae.</p> LIPs on Larval Feeding largerWorkCitation project eng; USA oceans -118.427 -118.091 33.6808 33.766 2020-01-01 2022-06-30 Southern California Bight 0 BCO-DMO catalogue of parameters from Quantification of ciliated band length per unit protein in early echinoderm larvae (protein data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/914280.rdf Name: Species Units: unitless Description: <p>Echinoderm species</p> http://lod.bco-dmo.org/id/dataset-parameter/914281.rdf Name: Age Units: days Description: <p>Age of larva in days post fertilization</p> http://lod.bco-dmo.org/id/dataset-parameter/914282.rdf Name: Dev Units: unitless Description: <p>Relative developmental stage of larva</p> http://lod.bco-dmo.org/id/dataset-parameter/914283.rdf Name: Beaker Units: unitless Description: <p>Beaker that larva was reared in unique for each species</p> http://lod.bco-dmo.org/id/dataset-parameter/914284.rdf Name: Sample Units: unitless Description: <p>Identifying number for protein sample. Unique for each Species age and beaker combination</p> http://lod.bco-dmo.org/id/dataset-parameter/914285.rdf Name: Average Units: nanograms (ng) Description: <p>Average protein content per larva in that sample</p> http://lod.bco-dmo.org/id/dataset-parameter/914286.rdf Name: sd Units: unitless Description: <p>Standard deviation for the protein content estimate for each sample based on three technical replicates</p> http://lod.bco-dmo.org/id/dataset-parameter/914287.rdf Name: geospatial_bound_N Units: decimal degrees Description: <p>The northern latitude of the bounding box that includes the collection site of all adult specimens</p> http://lod.bco-dmo.org/id/dataset-parameter/914288.rdf Name: geospatial_bound_S Units: decimal degrees Description: <p>The southern latitude of the bounding box that includes the collection site of all adult specimens</p> http://lod.bco-dmo.org/id/dataset-parameter/914289.rdf Name: geospatial_bound_E Units: decimal degrees Description: <p>The eastern longitude of the bounding box that includes the collection site of all adult specimens</p> http://lod.bco-dmo.org/id/dataset-parameter/914290.rdf Name: geospatial_bound_W Units: decimal degrees Description: <p>The western longitude of the bounding box that includes the collection site of all adult specimens</p> http://lod.bco-dmo.org/id/dataset-parameter/914291.rdf Name: experiment_location_lat Units: decimal degrees Description: <p>The latitude of the laboratory location where experiments took place</p> http://lod.bco-dmo.org/id/dataset-parameter/914292.rdf Name: experiment_location_long Units: decimal degrees Description: <p>The longitude of the laboratory location where experiments took place</p> http://lod.bco-dmo.org/id/dataset-parameter/914293.rdf Name: experiment_start Units: unitless Description: <p>The year and month (ISO format, yyyy-mm) when the first larval experiment began</p> http://lod.bco-dmo.org/id/dataset-parameter/914294.rdf Name: experiment_end Units: unitless Description: <p>The year and month (ISO format, yyyy-mm) when the final larval experiment concluded</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 44781 https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/a0a8b7df-848b-4918-aa90-5143b79351b3/content download https://doi.org/10.26008/1912/bco-dmo.914146.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Collection of adults and spawning:&amp;nbsp; &lt;/strong&gt;Adults of each species were collected from intertidal or shallow subtidal zones from various sites in Los Angeles County and transported to California State University Long Beach, where they were maintained in recirculating seawater tanks at 16 °C until their use in experiments. Experiments were carried out on one species at a time, depending on reproductive seasonality for that species.&lt;/p&gt; &lt;p&gt;Spawning was induced using standard methods (e.g., M. Strathmann, 1987). The echinoids &lt;em&gt;Dendraster excentricus&lt;/em&gt;, &lt;em&gt;Lytechnius pictus&lt;/em&gt;, &lt;em&gt;Strongylocentrotus purpuratus&lt;/em&gt; and &lt;em&gt;S. fragilis&lt;/em&gt; were induced to spawn via injection of 0.2-1.0 mL (depending on adult size) 0.53 M KCL into the perivisceral coelom. The asteroids &lt;em&gt;Patiria miniata&lt;/em&gt; and &lt;em&gt;Astropecten armatus&lt;/em&gt; were induced to spawn by injection of 1-3 mL 100 µM 1-methyladenine. The holothuroid &lt;em&gt;Apostichopus parvimensis&lt;/em&gt; was injected with 3 mL of 200 µM NGLWY-amide (Kato et al., 2009). The ophiuroid &lt;em&gt;Ophiothrix spiculata&lt;/em&gt; was exposed to 4 °C water in the dark for 15 minutes, then to room temperature water and sunlight for 15 minutes; this treatment was repeated for up to two hours (Selvakumaraswamy &amp;amp; Byrne, 2000). For all species, adults were each induced to spawn in their own isolated containers, allowing us to control subsequent fertilizations. Spawning continued until a minimum of three parents of each sex were obtained. Sperm from each spawning male was combined with eggs from each spawning female. Once the larvae reached the swimming stage (~24 hours), the offspring of all parents were combined to produce a genetically diverse population.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Culturing: &lt;/strong&gt;Larvae were distributed into seven replicate 2 l beakers, with a total of 500 larvae per beaker (for &lt;em&gt;S. purpuratus&lt;/em&gt;, 14 beakers of 500 larvae each were produced since greater numbers were needed for their protein analysis due to their small size). Larvae were fed 6000 cells ml&lt;sup&gt;−1&lt;/sup&gt; &lt;em&gt;Rhodomonas lens&lt;/em&gt; which were isolated from their growth medium via centrifugation, resuspended in FSW, and counted using a BD Accuri C6 flow cytometer. Cultures were maintained in the 16 °C environmental chamber and continuously stirred by a paddle system (Strathmann, 1987). Daily water changes began on the third day post fertilization (dpf), allowing larvae to develop without disturbance for a day while still in or just completing the pre-feeding period. To change the water, cultures were filtered through a 60 µm sieve to capture larvae. The sieve was submerged in shallow water while filtering so larvae were not exposed to air. Larvae were then gently rinsed from the filter with fresh FSW back into their cleaned beakers and fed.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sampling:&lt;/strong&gt; For all species except &lt;em&gt;D. excentricus&lt;/em&gt;, sampling for images and protein analysis occurred at 5 (“early-development”) and 10 dpf (“mid-development”). Sampling for &lt;em&gt;D. excentricus&lt;/em&gt; occurred at 3 and 5 dpf due to their more rapid development and early formation of the rudiment. While larvae were concentrated during the water change, we collected larvae using a pulled pipette. Depending on larval size and age, three samples of 30-80 larvae each were collected from each beaker and placed in 1.5 ml microcentrifuge tubes on ice. The remaining larvae after the early-development sampling were returned to their beaker and FSW was added to approximate a larval concentration of 0.25 larvae ml&lt;sup&gt;−1&lt;/sup&gt; based on an estimation of remaining larvae. Both &lt;em&gt;Strongylocentrotus &lt;/em&gt;species introduced small variations to these methods, since their small size necessitated experiments with greater numbers. Thus, for &lt;em&gt;S. purpuratus&lt;/em&gt;, 14 replicate beakers were made on 1 dpf so three samples of 150 larvae each could be taken on 5 dpf from seven of the beakers (and the remaining larvae in those beakers discarded), and the other seven beakers were used for the mid-development samples of 80 larvae each. &lt;em&gt;S. fragilis &lt;/em&gt;was included in this study opportunistically, so we were not able to repeat the experiment with greater numbers and instead had fewer replicates for &lt;em&gt;S. fragilis &lt;/em&gt;than for the other species. &lt;em&gt;S. fragilis&lt;/em&gt; had a total of 6 replicate cultures instead of 7, and the 10 dpf timepoint consisted of only two protein samples rather than three due to a shortage of larvae.&lt;br /&gt; &lt;br /&gt; The tubes containing larvae sampled for protein analysis were centrifuged to pellet the larvae. Water was aspirated gently using a pipette pulled to a tip size of 0.2 mm. Once aspiration was complete, the samples were stored at -80 °C for up to three months.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Protein Quantification: &lt;/strong&gt;To process the protein samples, we added 100 µl of 4 °C Nanopure water to each sample, and placed them on ice. The larval cells were then lysed using Fisherbrand polypropylene pestles designed to fit 1.5 ml microcentrifuge tubes. A Thermo Scientific Micro BCA Protein Assay Kit was used to determine the protein content of samples against a BSA (Bovine Serum Albumin) standard curve. 376 µl of 4 °C Nanopure water to each to provide adequate volume for the assay. The samples were vortexed for 10-15 seconds. 150 µl from the homogenized sample was then placed into three experimental wells of a 96-well plate to produce three technical replicates per sample. After addition of reagents and incubation according to the manufacturer’s instructions, the plates were read using a Biotek Synergy H1 Hybrid Microplate Reader. To calculate protein content per individual, the number of larvae per technical replicate was calculated as: larvae in technical replicate = larvae in original sample *&amp;nbsp; (150 µl technical replicate volume)/(476 µl sample volume).&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Protein&lt;/strong&gt;: Excel was used to generate the BSA standard curve for conversion of spectrophotometric absorption values to protein content. Two BSA standard ladders were produced per plate. The R&lt;sup&gt;2&lt;/sup&gt; value of the standard curve exceeded 0.98 each time the assay was performed. The sample readings were then calculated as ng protein per individual for each technical replicate, which were then averaged to yield a single value per sample. This resulted in three larval protein content estimates for each beaker at each developmental timepoint.&lt;br /&gt; &amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) * Changed species names to full species names: Astropecten armatus, Apostichopus parvimensis, Dendraster excentricus, Lytechinus pictus, Ophiothrix spiculata, Patiria miniata, Strongylocentrotus purpuratus * Adjusted parameter names to comply with database requirements Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact BD Accuri C6 Flow Cytometer (BD Biosciences) BD Accuri C6 Flow Cytometer (BD Biosciences) PI Supplied Instrument Name: BD Accuri C6 Flow Cytometer (BD Biosciences) PI Supplied Instrument Description:BD Accuri C6 Flow Cytometer (BD Biosciences) was used to determine concentration of Rhodomonas lens for larval feeding. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Biotek Synergy H1 Hybrid Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader PI Supplied Instrument Name: Biotek Synergy H1 Hybrid Microplate Reader PI Supplied Instrument Description:Biotek Synergy H1 Hybrid Microplate Reader, to measure spectrophotometric absorption values of protein samples and BSA standards. Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23. Thermo Scientific Micro BCA Protein Assay Kit Thermo Scientific Micro BCA Protein Assay Kit PI Supplied Instrument Name: Thermo Scientific Micro BCA Protein Assay Kit PI Supplied Instrument Description:Thermo Scientific Micro BCA Protein Assay Kit used to determine protein content of samples against a bovine serum albumin (BSA) standard curve. Instrument Name: Protein Assay Kit Instrument Short Name: Instrument Description: A protein assay kit is a collection of reagents and materials used to measure the concentration of protein in a solution.